StmaA.01152.a
Uroporphyrinogen III decarboxylase (EC 4.1.1.37) (hemE)
| CENTER ID: | StmaA.01152.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01152.a.B1.GE43290 | Full length(StmaA.01152.a) | 1 | 357 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01152.a.B1.PW38744 | Full length(StmaA.01152.a) | 1 | 357 |
Structures
6W2O
DEPOSITED: 3/6/2020
DETERMINATION: XRay
CLONE: StmaA.01152.a.B1.GE43290
PROTEIN: StmaA.01152.a.B1.PW38744
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3402 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSPLRNDRL LRALRREPVD CTPVWLMRQA GRYLPEYRAT RAKAGSFLAM AKNPEIACEV TLQPLRRFPL DAAILFSDIL TIPDAMGLEL YFVEGEGPKF RHPVRDEAAI ARLAVPDMEQ DLGYVMDAVR LIRRELDGQV PLIGFSGSPW TLACYMVEGG GSKDFARIKA MALNHPQALH RLLEVTTDAV IAYLGAQRAA GAQALQVFDT WGGVLSPAMY REFSLRYLQR IAEGLERGEG SERTPLILFG KGTGLHLEAL SQTGADALGL DWTLDLDEAM RRTGGRVALQ GNLDPTTLYA SPDAIAAAAA RVLDTYAAGN GGSREGHVFN LGHGMSPDMD PAHVQVLVDA VHAHSQR
NT Sequence
GTGACCAGCC CTCTCCGCAA CGATCGCCTG CTGCGCGCCC TGCGCCGCGA ACCGGTGGAC TGCACCCCCG TCTGGCTGAT GCGCCAGGCC GGCCGCTACC TGCCGGAGTA CCGCGCAACC CGGGCCAAGG CCGGCAGCTT CCTGGCCATG GCCAAGAACC CGGAGATCGC CTGCGAAGTC ACGCTGCAGC CGCTCCGCCG ATTCCCGCTG GACGCAGCCA TCCTGTTCTC GGACATCCTC ACCATTCCCG ATGCGATGGG CCTGGAGCTC TACTTTGTTG AAGGCGAAGG CCCGAAATTC CGTCATCCGG TGCGTGACGA AGCGGCCATC GCCCGGCTGG CGGTACCGGA CATGGAGCAG GACCTGGGCT ACGTGATGGA CGCGGTACGC CTGATCCGCC GCGAGCTGGA CGGCCAGGTA CCGTTGATCG GCTTCTCCGG CAGCCCCTGG ACACTGGCCT GCTACATGGT GGAAGGCGGC GGCAGCAAGG ATTTCGCGCG CATCAAGGCA ATGGCGCTGA ACCATCCGCA GGCCCTGCAC CGCCTGCTGG AGGTCACCAC CGACGCGGTG ATTGCGTACC TGGGCGCGCA GCGCGCGGCC GGTGCGCAGG CGCTGCAGGT GTTCGACACC TGGGGCGGCG TGCTGTCGCC GGCCATGTAC CGCGAGTTCT CGCTGCGCTA CCTGCAGCGC ATCGCCGAGG GCCTGGAGCG CGGCGAAGGC AGCGAGCGCA CGCCGCTGAT CCTGTTCGGC AAGGGTACCG GCCTGCACCT GGAAGCGCTG TCGCAGACCG GCGCCGATGC ACTGGGCCTG GACTGGACGC TGGACCTGGA CGAGGCGATG CGCCGCACCG GTGGCCGCGT CGCGCTGCAG GGCAACCTCG ACCCGACCAC GCTGTACGCC TCGCCGGATG CGATCGCCGC AGCCGCAGCG CGGGTGCTCG ACACCTACGC CGCCGGCAAT GGCGGTTCGC GCGAGGGCCA CGTGTTCAAC CTCGGCCACG GCATGTCGCC GGACATGGAC CCGGCCCACG TGCAGGTGCT GGTCGACGCG GTGCACGCGC ACAGCCAGCG C
Details for StmaA.01152.a.B1.GE43290
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMV TSPLRNDRLL RALRREPVDC TPVWLMRQAG RYLPEYRATR AKAGSFLAMA KNPEIACEVT LQPLRRFPLD AAILFSDILT IPDAMGLELY FVEGEGPKFR HPVRDEAAIA RLAVPDMEQD LGYVMDAVRL IRRELDGQVP LIGFSGSPWT LACYMVEGGG SKDFARIKAM ALNHPQALHR LLEVTTDAVI AYLGAQRAAG AQALQVFDTW GGVLSPAMYR EFSLRYLQRI AEGLERGEGS ERTPLILFGK GTGLHLEALS QTGADALGLD WTLDLDEAMR RTGGRVALQG NLDPTTLYAS PDAIAAAAAR VLDTYAAGNG GSREGHVFNL GHGMSPDMDP AHVQVLV
Validated NT Sequence
tcgaccagca cctgcacgtg ggccgggtcc atgtccggcg acatgccgtg gccgaggttg aacacgtggc cctcgcgcga accgccattg ccggcggcgt aggtgtcgag cacccgcgct gcggctgcgg cgatcgcatc cggcgaggcg tacagcgtgg tcgggtcgag gttgccctgc agcgcgacgc ggccaccggt gcggcgcatc gcctcgtcca ggtccagcgt ccagtccagg cccagtgcat cggcgccggt ctgcgacagc gcttccaggt gcaggccggt acccttgccg aacaggatca gcggcgtgcg ctcgctgcct tcgccgcgct ccaggccctc ggcgatgcgc tgcaggtagc gcagcgagaa ctcgcggtac atggccggcg acagcacgcc gccccaggtg tcgaacacct gcagcgcctg cgcaccggcc gcgcgctgcg cgcccaggta cgcaatcacc gcgtcggtgg tgacctccag caggcggtgc agggcctgcg gatggttcag cgccattgcc ttgatgcgcg cgaaatcctt gctgccgccg ccttccacca tgtagcaggc cagtgtccag gggctgccgg agaagccgat caacggtacc tggccgtcca gctcgcggcg gatcaggcgt accgcgtcca tcacgtagcc caggtcctgc tccatgtccg gtaccgccag ccgggcgatg gccgcttcgt cacgcaccgg atgacggaat ttcgggcctt cgccttcaac aaagtagagc tccaggccca tcgcatcggg aatggtgagg atgtccgaga acaggatggc tgcgtccagc gggaatcggc ggagcggctg cagcgtgact tcgcaggcga tctccgggtt cttggccatg gccaggaagc tgccggcctt ggcccgggtt gcgcggtact ccggcaggta gcggccggcc tggcgcatca gccagacggg ggtgcagtcc accggttcgc ggcgcagggc gcgcagcagg cgatcgttgc ggagagggct ggtcaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT SPLRNDRLLR ALRREPVDCT PVWLMRQAGR YLPEYRATRA KAGSFLAMAK NPEIACEVTL QPLRRFPLDA AILFSDILTI PDAMGLELYF VEGEGPKFRH PVRDEAAIAR LAVPDMEQDL GYVMDAVRLI RRELDGQVPL IGFSGSPWTL ACYMVEGGGS KDFARIKAMA LNHPQALHRL LEVTTDAVIA YLGAQRAAGA QALQVFDTWG GVLSPAMYRE FSLRYLQRIA EGLERGEGSE RTPLILFGKG TGLHLEALSQ TGADALGLDW TLDLDEAMRR TGGRVALQGN LDPTTLYASP DAIAAAAARV LDTYAAGNGG SREGHVFNLG HGMSPDMDPA HVQVLVDAVH AHSQR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacca gccctctccg caacgatcgc ctgctgcgcg ccctgcgccg cgaaccggtg gactgcaccc ccgtctggct gatgcgccag gccggccgct acctgccgga gtaccgcgca acccgggcca aggccggcag cttcctggcc atggccaaga acccggagat cgcctgcgaa gtcacgctgc agccgctccg ccgattcccg ctggacgcag ccatcctgtt ctcggacatc ctcaccattc ccgatgcgat gggcctggag ctctactttg ttgaaggcga aggcccgaaa ttccgtcatc cggtgcgtga cgaagcggcc atcgcccggc tggcggtacc ggacatggag caggacctgg gctacgtgat ggacgcggta cgcctgatcc gccgcgagct ggacggccag gtaccgttga tcggcttctc cggcagcccc tggacactgg cctgctacat ggtggaaggc ggcggcagca aggatttcgc gcgcatcaag gcaatggcgc tgaaccatcc gcaggccctg caccgcctgc tggaggtcac caccgacgcg gtgattgcgt acctgggcgc gcagcgcgcg gccggtgcgc aggcgctgca ggtgttcgac acctggggcg gcgtgctgtc gccggccatg taccgcgagt tctcgctgcg ctacctgcag cgcatcgccg agggcctgga gcgcggcgaa ggcagcgagc gcacgccgct gatcctgttc ggcaagggta ccggcctgca cctggaagcg ctgtcgcaga ccggcgccga tgcactgggc ctggactgga cgctggacct ggacgaggcg atgcgccgca ccggtggccg cgtcgcgctg cagggcaacc tcgacccgac cacgctgtac gcctcgccgg atgcgatcgc cgcagccgca gcgcgggtgc tcgacaccta cgccgccggc aatggcggtt cgcgcgaggg ccacgtgttc aacctcggcc acggcatgtc gccggacatg gacccggccc acgtgcaggt gctggtcgac gcggtgcacg cgcacagcca gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for StmaA.01152.a.B1.PW38744
| PURIFICATION DATe: | 10/25/2019 |
| CONCENTRATION: | 20.4mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV TSPLRNDRLL RALRREPVDC TPVWLMRQAG RYLPEYRATR AKAGSFLAMA KNPEIACEVT LQPLRRFPLD AAILFSDILT IPDAMGLELY FVEGEGPKFR HPVRDEAAIA RLAVPDMEQD LGYVMDAVRL IRRELDGQVP LIGFSGSPWT LACYMVEGGG SKDFARIKAM ALNHPQALHR LLEVTTDAVI AYLGAQRAAG AQALQVFDTW GGVLSPAMYR EFSLRYLQRI AEGLERGEGS ERTPLILFGK GTGLHLEALS QTGADALGLD WTLDLDEAMR RTGGRVALQG NLDPTTLYAS PDAIAAAAAR VLDTYAAGNG GSREGHVFNL GHGMSPDMDP AHVQVLV
Validated NT Sequence
tcgaccagca cctgcacgtg ggccgggtcc atgtccggcg acatgccgtg gccgaggttg aacacgtggc cctcgcgcga accgccattg ccggcggcgt aggtgtcgag cacccgcgct gcggctgcgg cgatcgcatc cggcgaggcg tacagcgtgg tcgggtcgag gttgccctgc agcgcgacgc ggccaccggt gcggcgcatc gcctcgtcca ggtccagcgt ccagtccagg cccagtgcat cggcgccggt ctgcgacagc gcttccaggt gcaggccggt acccttgccg aacaggatca gcggcgtgcg ctcgctgcct tcgccgcgct ccaggccctc ggcgatgcgc tgcaggtagc gcagcgagaa ctcgcggtac atggccggcg acagcacgcc gccccaggtg tcgaacacct gcagcgcctg cgcaccggcc gcgcgctgcg cgcccaggta cgcaatcacc gcgtcggtgg tgacctccag caggcggtgc agggcctgcg gatggttcag cgccattgcc ttgatgcgcg cgaaatcctt gctgccgccg ccttccacca tgtagcaggc cagtgtccag gggctgccgg agaagccgat caacggtacc tggccgtcca gctcgcggcg gatcaggcgt accgcgtcca tcacgtagcc caggtcctgc tccatgtccg gtaccgccag ccgggcgatg gccgcttcgt cacgcaccgg atgacggaat ttcgggcctt cgccttcaac aaagtagagc tccaggccca tcgcatcggg aatggtgagg atgtccgaga acaggatggc tgcgtccagc gggaatcggc ggagcggctg cagcgtgact tcgcaggcga tctccgggtt cttggccatg gccaggaagc tgccggcctt ggcccgggtt gcgcggtact ccggcaggta gcggccggcc tggcgcatca gccagacggg ggtgcagtcc accggttcgc ggcgcagggc gcgcagcagg cgatcgttgc ggagagggct ggtcaccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT SPLRNDRLLR ALRREPVDCT PVWLMRQAGR YLPEYRATRA KAGSFLAMAK NPEIACEVTL QPLRRFPLDA AILFSDILTI PDAMGLELYF VEGEGPKFRH PVRDEAAIAR LAVPDMEQDL GYVMDAVRLI RRELDGQVPL IGFSGSPWTL ACYMVEGGGS KDFARIKAMA LNHPQALHRL LEVTTDAVIA YLGAQRAAGA QALQVFDTWG GVLSPAMYRE FSLRYLQRIA EGLERGEGSE RTPLILFGKG TGLHLEALSQ TGADALGLDW TLDLDEAMRR TGGRVALQGN LDPTTLYASP DAIAAAAARV LDTYAAGNGG SREGHVFNLG HGMSPDMDPA HVQVLVDAVH AHSQR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACCA GCCCTCTCCG CAACGATCGC CTGCTGCGCG CCCTGCGCCG CGAACCGGTG GACTGCACCC CCGTCTGGCT GATGCGCCAG GCCGGCCGCT ACCTGCCGGA GTACCGCGCA ACCCGGGCCA AGGCCGGCAG CTTCCTGGCC ATGGCCAAGA ACCCGGAGAT CGCCTGCGAA GTCACGCTGC AGCCGCTCCG CCGATTCCCG CTGGACGCAG CCATCCTGTT CTCGGACATC CTCACCATTC CCGATGCGAT GGGCCTGGAG CTCTACTTTG TTGAAGGCGA AGGCCCGAAA TTCCGTCATC CGGTGCGTGA CGAAGCGGCC ATCGCCCGGC TGGCGGTACC GGACATGGAG CAGGACCTGG GCTACGTGAT GGACGCGGTA CGCCTGATCC GCCGCGAGCT GGACGGCCAG GTACCGTTGA TCGGCTTCTC CGGCAGCCCC TGGACACTGG CCTGCTACAT GGTGGAAGGC GGCGGCAGCA AGGATTTCGC GCGCATCAAG GCAATGGCGC TGAACCATCC GCAGGCCCTG CACCGCCTGC TGGAGGTCAC CACCGACGCG GTGATTGCGT ACCTGGGCGC GCAGCGCGCG GCCGGTGCGC AGGCGCTGCA GGTGTTCGAC ACCTGGGGCG GCGTGCTGTC GCCGGCCATG TACCGCGAGT TCTCGCTGCG CTACCTGCAG CGCATCGCCG AGGGCCTGGA GCGCGGCGAA GGCAGCGAGC GCACGCCGCT GATCCTGTTC GGCAAGGGTA CCGGCCTGCA CCTGGAAGCG CTGTCGCAGA CCGGCGCCGA TGCACTGGGC CTGGACTGGA CGCTGGACCT GGACGAGGCG ATGCGCCGCA CCGGTGGCCG CGTCGCGCTG CAGGGCAACC TCGACCCGAC CACGCTGTAC GCCTCGCCGG ATGCGATCGC CGCAGCCGCA GCGCGGGTGC TCGACACCTA CGCCGCCGGC AATGGCGGTT CGCGCGAGGG CCACGTGTTC AACCTCGGCC ACGGCATGTC GCCGGACATG GACCCGGCCC ACGTGCAGGT GCTGGTCGAC GCGGTGCACG CGCACAGCCA GCGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT