StmaA.01095.b
(2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) (ispA)
| CENTER ID: | StmaA.01095.b |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01095.b.B1.GE43287 | Full length(StmaA.01095.b) | 1 | 292 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01095.b.B1.PW38725 | Full length(StmaA.01095.b) | 1 | 292 |
Structures
7LBJ
DEPOSITED: 1/8/2021
DETERMINATION: XRay
CLONE: StmaA.01095.b.B1.GE43287
PROTEIN: StmaA.01095.b.B1.PW38725
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3252 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAEVLFARW RDRIESQLDA ALPSPAEAPQ RLHQAMRYSV LGGGKRMRPL LVYASGHLFG TQPESLDAAA MSVELIHAYS LVHDDLPAMD DDALRRGKPT THVAFDEATA ILAGDALQTR AFGLLADAPL PATLRVACLQ TLAHASGASG MCGGQALDID ATGQQQTLAA LTRMHALKTG ALIRAAVRMG ALCGQAHEPQ LAQLDSFADA LGLAFQVRDD ILDVEASSEQ LGKTAGKDQA QDKSTFPALL GMDGAKAQLH ELAARMQSIL ARYGEEADAL RALATLAVER DH
NT Sequence
ATGACGGCTG AGGTTCTGTT CGCGCGCTGG CGTGACCGTA TCGAAAGTCA GCTCGATGCC GCCCTGCCCT CGCCTGCCGA GGCGCCGCAG CGCCTGCATC AGGCCATGCG CTATTCGGTG CTTGGTGGCG GCAAGCGCAT GCGGCCCCTG CTGGTCTACG CCAGTGGTCA CCTGTTCGGT ACGCAGCCGG AAAGCCTGGA TGCGGCGGCC ATGTCCGTCG AGCTGATCCA TGCCTATTCG CTGGTGCATG ACGACCTTCC GGCGATGGAT GACGACGCCC TGCGTCGCGG CAAGCCCACC ACGCACGTCG CCTTCGACGA AGCCACCGCG ATCCTTGCTG GCGACGCCCT GCAGACCCGC GCCTTCGGCC TGCTGGCCGA TGCGCCGCTG CCGGCCACGT TGCGCGTGGC CTGCCTGCAG ACCCTGGCCC ACGCCTCAGG CGCGTCCGGC ATGTGTGGCG GCCAGGCGCT GGACATCGAT GCCACCGGCC AGCAGCAGAC GCTGGCGGCG CTGACCCGCA TGCACGCGCT GAAGACCGGC GCGCTGATCC GCGCGGCAGT GCGCATGGGC GCGCTGTGCG GCCAGGCCCA CGAACCGCAG CTGGCCCAGC TCGACAGTTT CGCCGATGCA CTCGGCCTGG CCTTCCAGGT CCGCGATGAC ATCCTCGATG TCGAAGCCAG TTCCGAACAG CTGGGCAAGA CTGCCGGCAA GGACCAGGCG CAGGACAAGA GCACCTTCCC CGCCCTGCTT GGCATGGACG GCGCAAAGGC CCAGCTGCAC GAGCTCGCAG CGCGCATGCA GTCGATCCTG GCCAGGTATG GCGAGGAAGC CGACGCGCTG CGCGCGCTGG CGACGCTGGC GGTGGAACGC GATCAC
Details for StmaA.01095.b.B1.GE43287
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 32kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT AEVLFARWRD RIESQLDAAL PSPAEAPQRL HQAMRYSVLG GGKRMRPLLV YASGHLFGTQ PESLDAAAMS VELIHAYSLV HDDLPAMDDD ALRRGKPTTH VAFDEATAIL AGDALQTRAF GLLADAPLPA TLRVACLQTL AHASGASGMC GGQALDIDAT GQQQTLAALT RMHALKTGAL IRAAVRMGAL CGQAHEPQLA QLDSFADALG LAFQVRDDIL DVEASSEQLG KTAGKDQAQD KSTFPALLGM DGAKAQLHEL AARMQSILAR YGEEADALRA LAXXAVERDH
Validated NT Sequence
tactcactta gtgatcgcgt tccaccgcnn nnnncgccag cgcgcgcagc gcgtcggctt cctcgccata cctggccagg atcgactgca tgcgcgctgc gagctcgtgc agctgggcct ttgcgccgtc catgccaagc agggcgggga aggtgctctt gtcctgcgcc tggtccttgc cggcagtctt gcccagctgt tcggaactgg cttcgacatc gaggatgtca tcgcggacct ggaaggccag gccgagtgca tcggcgaaac tgtcgagctg ggccagctgc ggttcgtggg cctggccgca cagcgcgccc atgcgcactg ccgcgcggat cagcgcgccg gtcttcagcg cgtgcatgcg ggtcagcgcc gccagcgtct gctgctggcc ggtggcatcg atgtccagcg cctggccgcc acacatgccg gacgcgcctg aggcgtgggc cagggtctgc aggcaggcca cgcgcaacgt ggccggcagc ggcgcatcgg ccagcaggcc gaaggcgcgg gtctgcaggg cgtcgccagc aaggatcgcg gtggcttcgt cgaaggcgac gtgcgtggtg ggcttgccgc gacgcagggc gtcgtcatcc atcgccggaa ggtcgtcatg caccagcgaa taggcatgga tcagctcgac ggacatggcc gccgcatcca ggctttccgg ctgcgtaccg aacaggtgac cactggcgta gaccagcagg ggccgcatgc gcttgccgcc accaagcacc gaatagcgca tggcctgatg caggcgctgc ggcgcctcgg caggcgaggg cagggcggca tcgagctgac tttcgatacg gtcacgccag cgcgcgaaca gaacctcagc cgtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT AEVLFARWRD RIESQLDAAL PSPAEAPQRL HQAMRYSVLG GGKRMRPLLV YASGHLFGTQ PESLDAAAMS VELIHAYSLV HDDLPAMDDD ALRRGKPTTH VAFDEATAIL AGDALQTRAF GLLADAPLPA TLRVACLQTL AHASGASGMC GGQALDIDAT GQQQTLAALT RMHALKTGAL IRAAVRMGAL CGQAHEPQLA QLDSFADALG LAFQVRDDIL DVEASSEQLG KTAGKDQAQD KSTFPALLGM DGAKAQLHEL AARMQSILAR YGEEADALRA LATLAVERDH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ctgaggttct gttcgcgcgc tggcgtgacc gtatcgaaag tcagctcgat gccgccctgc cctcgcctgc cgaggcgccg cagcgcctgc atcaggccat gcgctattcg gtgcttggtg gcggcaagcg catgcggccc ctgctggtct acgccagtgg tcacctgttc ggtacgcagc cggaaagcct ggatgcggcg gccatgtccg tcgagctgat ccatgcctat tcgctggtgc atgacgacct tccggcgatg gatgacgacg ccctgcgtcg cggcaagccc accacgcacg tcgccttcga cgaagccacc gcgatccttg ctggcgacgc cctgcagacc cgcgccttcg gcctgctggc cgatgcgccg ctgccggcca cgttgcgcgt ggcctgcctg cagaccctgg cccacgcctc aggcgcgtcc ggcatgtgtg gcggccaggc gctggacatc gatgccaccg gccagcagca gacgctggcg gcgctgaccc gcatgcacgc gctgaagacc ggcgcgctga tccgcgcggc agtgcgcatg ggcgcgctgt gcggccaggc ccacgaaccg cagctggccc agctcgacag tttcgccgat gcactcggcc tggccttcca ggtccgcgat gacatcctcg atgtcgaagc cagttccgaa cagctgggca agactgccgg caaggaccag gcgcaggaca agagcacctt ccccgccctg cttggcatgg acggcgcaaa ggcccagctg cacgagctcg cagcgcgcat gcagtcgatc ctggccaggt atggcgagga agccgacgcg ctgcgcgcgc tggcgacgct ggcggtggaa cgcgatcact gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for StmaA.01095.b.B1.PW38725
| PURIFICATION DATe: | 10/18/2019 |
| CONCENTRATION: | 18.4mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT AEVLFARWRD RIESQLDAAL PSPAEAPQRL HQAMRYSVLG GGKRMRPLLV YASGHLFGTQ PESLDAAAMS VELIHAYSLV HDDLPAMDDD ALRRGKPTTH VAFDEATAIL AGDALQTRAF GLLADAPLPA TLRVACLQTL AHASGASGMC GGQALDIDAT GQQQTLAALT RMHALKTGAL IRAAVRMGAL CGQAHEPQLA QLDSFADALG LAFQVRDDIL DVEASSEQLG KTAGKDQAQD KSTFPALLGM DGAKAQLHEL AARMQSILAR YGEEADALRA LAXXAVERDH
Validated NT Sequence
tactcactta gtgatcgcgt tccaccgcnn nnnncgccag cgcgcgcagc gcgtcggctt cctcgccata cctggccagg atcgactgca tgcgcgctgc gagctcgtgc agctgggcct ttgcgccgtc catgccaagc agggcgggga aggtgctctt gtcctgcgcc tggtccttgc cggcagtctt gcccagctgt tcggaactgg cttcgacatc gaggatgtca tcgcggacct ggaaggccag gccgagtgca tcggcgaaac tgtcgagctg ggccagctgc ggttcgtggg cctggccgca cagcgcgccc atgcgcactg ccgcgcggat cagcgcgccg gtcttcagcg cgtgcatgcg ggtcagcgcc gccagcgtct gctgctggcc ggtggcatcg atgtccagcg cctggccgcc acacatgccg gacgcgcctg aggcgtgggc cagggtctgc aggcaggcca cgcgcaacgt ggccggcagc ggcgcatcgg ccagcaggcc gaaggcgcgg gtctgcaggg cgtcgccagc aaggatcgcg gtggcttcgt cgaaggcgac gtgcgtggtg ggcttgccgc gacgcagggc gtcgtcatcc atcgccggaa ggtcgtcatg caccagcgaa taggcatgga tcagctcgac ggacatggcc gccgcatcca ggctttccgg ctgcgtaccg aacaggtgac cactggcgta gaccagcagg ggccgcatgc gcttgccgcc accaagcacc gaatagcgca tggcctgatg caggcgctgc ggcgcctcgg caggcgaggg cagggcggca tcgagctgac tttcgatacg gtcacgccag cgcgcgaaca gaacctcagc cgtcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMT AEVLFARWRD RIESQLDAAL PSPAEAPQRL HQAMRYSVLG GGKRMRPLLV YASGHLFGTQ PESLDAAAMS VELIHAYSLV HDDLPAMDDD ALRRGKPTTH VAFDEATAIL AGDALQTRAF GLLADAPLPA TLRVACLQTL AHASGASGMC GGQALDIDAT GQQQTLAALT RMHALKTGAL IRAAVRMGAL CGQAHEPQLA QLDSFADALG LAFQVRDDIL DVEASSEQLG KTAGKDQAQD KSTFPALLGM DGAKAQLHEL AARMQSILAR YGEEADALRA LATLAVERDH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGG CTGAGGTTCT GTTCGCGCGC TGGCGTGACC GTATCGAAAG TCAGCTCGAT GCCGCCCTGC CCTCGCCTGC CGAGGCGCCG CAGCGCCTGC ATCAGGCCAT GCGCTATTCG GTGCTTGGTG GCGGCAAGCG CATGCGGCCC CTGCTGGTCT ACGCCAGTGG TCACCTGTTC GGTACGCAGC CGGAAAGCCT GGATGCGGCG GCCATGTCCG TCGAGCTGAT CCATGCCTAT TCGCTGGTGC ATGACGACCT TCCGGCGATG GATGACGACG CCCTGCGTCG CGGCAAGCCC ACCACGCACG TCGCCTTCGA CGAAGCCACC GCGATCCTTG CTGGCGACGC CCTGCAGACC CGCGCCTTCG GCCTGCTGGC CGATGCGCCG CTGCCGGCCA CGTTGCGCGT GGCCTGCCTG CAGACCCTGG CCCACGCCTC AGGCGCGTCC GGCATGTGTG GCGGCCAGGC GCTGGACATC GATGCCACCG GCCAGCAGCA GACGCTGGCG GCGCTGACCC GCATGCACGC GCTGAAGACC GGCGCGCTGA TCCGCGCGGC AGTGCGCATG GGCGCGCTGT GCGGCCAGGC CCACGAACCG CAGCTGGCCC AGCTCGACAG TTTCGCCGAT GCACTCGGCC TGGCCTTCCA GGTCCGCGAT GACATCCTCG ATGTCGAAGC CAGTTCCGAA CAGCTGGGCA AGACTGCCGG CAAGGACCAG GCGCAGGACA AGAGCACCTT CCCCGCCCTG CTTGGCATGG ACGGCGCAAA GGCCCAGCTG CACGAGCTCG CAGCGCGCAT GCAGTCGATC CTGGCCAGGT ATGGCGAGGA AGCCGACGCG CTGCGCGCGC TGGCGACGCT GGCGGTGGAA CGCGATCACT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT