StmaA.01067.a

DNA-directed RNA polymerase alpha subunit (EC 2.7.7.6) (rpoA)

CENTER ID: StmaA.01067.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01067.a.B1.GE43285 Full length(StmaA.01067.a) 1 332
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.898

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVTANQVLR PRGPQIERLT DTRAKVVIEP LERGYGHTLG NALRRVLLSS IPGFAITEVE IDGVLHEYTT VEGLQEDVLE VLLNLKDVAI RMHSGDSATL SLSKQGPGVV TAADIKVDHN VEILNGDHVI CHLTKDTAIN MRLKIERGFG YQPAAARRRP DEETRAIGRL VLDASFSPVR RVAYAVEAAR VEQRTDLDKL VIDIETNGTI DAEEAVRTAA DILSDQLSVF GDFTHRDRGA AKPANNGVDP VLLRPIDDLE LTVRSANCLK AESIYYIGDL IQKTEVELLK TPNLGKKSLT EIKEVLAQRG LSLGMKLENW PPAGVASHGM LG
NT Sequence
ATGACGGTTA CCGCCAACCA GGTTCTGCGT CCTCGCGGTC CGCAGATCGA ACGCCTTACC GACACCCGTG CAAAGGTCGT TATCGAACCT TTGGAGCGGG GTTACGGGCA TACGCTGGGT AATGCCCTGC GTCGCGTGCT GCTGTCGTCC ATCCCGGGCT TCGCCATCAC GGAAGTCGAA ATCGACGGCG TGTTGCATGA GTACACCACG GTCGAAGGTC TGCAGGAGGA CGTCCTCGAA GTCCTGCTGA ACCTCAAGGA CGTGGCCATC CGTATGCACT CCGGCGACAG CGCCACCCTG TCCCTGTCCA AGCAGGGCCC GGGCGTTGTC ACCGCTGCCG ACATCAAGGT CGACCACAAT GTGGAGATCC TGAACGGCGA CCATGTGATC TGCCACCTGA CCAAGGACAC GGCAATCAAC ATGCGTCTGA AGATCGAACG TGGTTTCGGC TACCAGCCGG CTGCCGCGCG TCGTCGTCCG GACGAAGAAA CCCGTGCCAT CGGCCGCCTG GTCCTGGATG CCTCGTTCTC GCCGGTCCGC CGCGTCGCCT ATGCCGTGGA AGCGGCCCGC GTCGAACAGC GTACCGACCT GGACAAGCTG GTCATCGATA TCGAGACCAA CGGCACCATC GACGCCGAGG AAGCCGTGCG CACCGCCGCC GACATCCTCA GCGACCAGCT GTCGGTGTTC GGTGACTTCA CCCACCGCGA CCGCGGTGCG GCCAAGCCGG CAAACAACGG CGTGGATCCG GTGCTGCTGC GCCCGATCGA CGATCTGGAG CTGACCGTGC GTTCGGCCAA CTGCCTGAAG GCTGAAAGCA TCTACTACAT CGGCGATCTG ATCCAGAAGA CCGAAGTGGA GCTGCTGAAG ACCCCGAACC TGGGCAAGAA GTCGCTCACC GAGATCAAGG AAGTGCTGGC TCAGCGCGGC CTGTCGCTCG GCATGAAGCT GGAGAACTGG CCGCCGGCCG GTGTCGCCAG CCACGGCATG CTGGGC
Details for StmaA.01067.a.B1.GE43285
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 37kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT VTXNXVLRPR GPQIERLTDT RAKVVIEPLE RGYGHTLGNA LRRVLLSSIP GFAITEVEID GVLHEYTTVE GLQEDVLEVL LNLKDVAIRM HSGDSATLSL SKQGPGVVTA ADIKVDHNVE ILNGDHVICH LTKDTAINMR LKIERGFGYQ PAAARRRPDE ETRAIGRLVL DASFSPVRRV AYAVEAARVE QRTDLDKLVI DIETNGTIDA EEAVRTAADI LSDQLSVFGD FTHRDRGAAK PANNGVDPVL LRPIDDLELT VRSANCXKAE SIYYIGDLIQ KTEVELXKTP NLGKKSLTEI KEVLAQRGLX LGMXXENWPP AGXXSHGXLG
Validated NT Sequence
atggctcacc accaccacca ccatatgacs gttaccgmca accasgttct gcgtcctcgc ggtccgcaga tcgaacgcct taccgacacc cgtgcaaagg tcgttatcga acctttggag cggggttacg ggcatacgct gggtaatgcc ctgcgtcgcg tgctgctgtc gtccatcccg ggcttcgcca tcackgaagt sgaaatcgac ggcgtgttgc atgagtacac cacggtcgaa ggtctgcagg aggacgtcct cgaagtcctg ctgaacctca aggacgtggc catccgtatg cactccggcg acagcgccac cctgtccctg tccaagcagg gcccgggcgt tgtcaccgct gccgacatca aggtcgacca caatgtggag atcctgaacg gcgaccatgt gatctgccac ctgaccaagg acacggcaat caacatgcgt ctgaagatcg aacgtggttt cggctaccag ccggctgccg cgcgtcgtcg tccggacgaa gaaacccgtg ccatcggccg cctggtcctg gatgcctcgt tctcgccggt ccgccgcgtc gcctatgccg tggaagcggc ccgcgtcgaa cagcgtaccg acctggacaa gctggtcatc gatatcgaga ccaacggcac catcgacgcc gaggaagccg tgcgcaccgc cgccgacatc ctcagcgacc agctgtcggt gttcggtgac ttcacccacc gcgaccgcgg tgcggccaag ccggcaaaca acggcgtgga tccggtgctg ctgcgcccga tcgacgatct ggagctgacc gtgcgttcgg ccaactgcst gaaggctgaa agcatctact acatcggcga tctgatccag aagaccgaag tggagctgst gaagaccccg aacctgggca agaagtcgct caccgagatc aaggaagtgc tggctcagcg cggcctgtsg ctcggcatga asmtggagaa ctggccgccg gccggtgncn ncagccacgg caksctgggc taa
Expected Protein Sequence
MAHHHHHHMT VTANQVLRPR GPQIERLTDT RAKVVIEPLE RGYGHTLGNA LRRVLLSSIP GFAITEVEID GVLHEYTTVE GLQEDVLEVL LNLKDVAIRM HSGDSATLSL SKQGPGVVTA ADIKVDHNVE ILNGDHVICH LTKDTAINMR LKIERGFGYQ PAAARRRPDE ETRAIGRLVL DASFSPVRRV AYAVEAARVE QRTDLDKLVI DIETNGTIDA EEAVRTAADI LSDQLSVFGD FTHRDRGAAK PANNGVDPVL LRPIDDLELT VRSANCLKAE SIYYIGDLIQ KTEVELLKTP NLGKKSLTEI KEVLAQRGLS LGMKLENWPP AGVASHGMLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgg ttaccgccaa ccaggttctg cgtcctcgcg gtccgcagat cgaacgcctt accgacaccc gtgcaaaggt cgttatcgaa cctttggagc ggggttacgg gcatacgctg ggtaatgccc tgcgtcgcgt gctgctgtcg tccatcccgg gcttcgccat cacggaagtc gaaatcgacg gcgtgttgca tgagtacacc acggtcgaag gtctgcagga ggacgtcctc gaagtcctgc tgaacctcaa ggacgtggcc atccgtatgc actccggcga cagcgccacc ctgtccctgt ccaagcaggg cccgggcgtt gtcaccgctg ccgacatcaa ggtcgaccac aatgtggaga tcctgaacgg cgaccatgtg atctgccacc tgaccaagga cacggcaatc aacatgcgtc tgaagatcga acgtggtttc ggctaccagc cggctgccgc gcgtcgtcgt ccggacgaag aaacccgtgc catcggccgc ctggtcctgg atgcctcgtt ctcgccggtc cgccgcgtcg cctatgccgt ggaagcggcc cgcgtcgaac agcgtaccga cctggacaag ctggtcatcg atatcgagac caacggcacc atcgacgccg aggaagccgt gcgcaccgcc gccgacatcc tcagcgacca gctgtcggtg ttcggtgact tcacccaccg cgaccgcggt gcggccaagc cggcaaacaa cggcgtggat ccggtgctgc tgcgcccgat cgacgatctg gagctgaccg tgcgttcggc caactgcctg aaggctgaaa gcatctacta catcggcgat ctgatccaga agaccgaagt ggagctgctg aagaccccga acctgggcaa gaagtcgctc accgagatca aggaagtgct ggctcagcgc ggcctgtcgc tcggcatgaa gctggagaac tggccgccgg ccggtgtcgc cagccacggc atgctgggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat