SSGCID: Target Page

StmaA.01059.a

Methionyl-tRNA formyltransferase (EC 2.1.2.9) (fmt)

CENTER ID:	StmaA.01059.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.01059.a.B1.GE43283	Full length(StmaA.01059.a)		1	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3947

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSLRAAARH HEVVAVYTQP DRPAGRGRGL APSPVKLEAV ARGIPVYQPE SLKDAAAQQQ LRDLQPDLMV VVAYGLILPK AVLAIPTHGC WNVHASLLPR WRGAAPIQRA IQAGDAKTGV CLMQMEAGLD TGPVLLHQEL PIASTDTGGQ LHDKLAELGA QVLSDGLGLL RAGIKPIARP QPEQGVTYAH KLDKAEARLD WAQDADALAR TVRAFNPWPI AEATLAGERV RIHGAVALEA DHGQAPGTVL AAGRDGIDIA CGQGALRLRV LQREGGKAIT AADYLNARRD LRVGA

NT Sequence

GTGTCGTCGC TGCGCGCGGC TGCGCGCCAC CATGAGGTCG TGGCCGTCTA TACCCAGCCT GATCGCCCGG CCGGCCGAGG CCGTGGCCTG GCGCCTTCGC CGGTCAAGCT CGAAGCCGTG GCCCGTGGCA TTCCGGTCTA CCAGCCGGAG TCGCTGAAGG ACGCGGCCGC CCAGCAGCAG TTGCGCGACC TGCAGCCGGA CCTGATGGTG GTGGTGGCCT ATGGCCTGAT CCTGCCCAAG GCAGTACTGG CCATCCCGAC CCATGGTTGC TGGAACGTGC ATGCCTCGCT GCTGCCGCGC TGGCGCGGCG CTGCGCCGAT CCAGCGTGCG ATCCAGGCTG GTGACGCGAA GACCGGGGTG TGCCTGATGC AGATGGAGGC TGGCCTGGAT ACCGGCCCGG TGCTGCTGCA CCAGGAGCTG CCGATCGCCT CCACCGATAC CGGTGGCCAG CTGCACGACA AGCTGGCCGA GCTTGGTGCC CAGGTGTTGT CCGATGGCCT GGGCCTGCTG CGCGCGGGCA TCAAGCCGAT CGCGCGACCG CAGCCGGAAC AGGGCGTGAC GTACGCGCAC AAGCTGGACA AGGCCGAAGC CAGGCTGGAC TGGGCGCAGG ACGCCGACGC GCTGGCGCGT ACCGTGCGCG CGTTCAATCC GTGGCCGATC GCCGAGGCAA CGCTGGCTGG CGAACGCGTG CGCATCCATG GTGCGGTGGC ACTGGAGGCA GACCACGGCC AGGCGCCGGG CACCGTGCTG GCCGCCGGCC GCGACGGCAT CGACATTGCC TGTGGCCAGG GCGCGCTGCG CCTGCGCGTG CTGCAGCGTG AAGGCGGCAA GGCGATCACC GCTGCCGATT ACCTCAACGC TCGCCGCGAC CTGCGCGTGG GGGCA

Details for StmaA.01059.a.B1.GE43283

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	32kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHHM VSSLRAAARH HEVVAVYTQP DRPAGRGRGL APSPVKLEAV ARGIPVYQPE SLKDAAAQQQ LRDLQPDLMV VVAYGLILPK AVLAIPTHGC WNVHASLLPR WRGAAPIQRA IQAGDAKTGV CLMQMEAGLD TGPVLLHQEL PIASTDTGGQ LHDKLAELGA QVLSDGLGLL RAGIKPIARP QPEQGVTYAH KLDKAEARLD WAQDADALAR TVRAFNPWPI AEATLAGERV RIHGAVALEA DHGQAPGTVL AAGRDGIDIA CGQGALRLRV LQREGGKAIT AADYLNARRD LRVGA

Validated NT Sequence

atcttactca cttatgcccc cacgcgcagg tcgcggcgag cgttgaggta atcggcagcg gtgatcgcct tgccgccttc acgctgcagc acgcgcaggc gcagcgcgcc ctggccacag gcaatgtcga tgccgtcgcg gccggcggcc agcacggtgc ccggcgcctg gccgtggtct gcctccagtg ccaccgcacc atggatgcgc acgcgttcgc cagccagcgt tgcctcggcg atcggccacg gattgaacgc gcgcacggta cgcgccagcg cgtcggcgtc ctgcgcccag tccagcctgg cttcggcctt gtccagcttg tgcgcgtacg tcacgccctg ttccggctgc ggtcgcgcga tcggcttgat gcccgcgcgc agcaggccca ggccatcgga caacacctgg gcaccaagct cggccagctt gtcgtgcagc tggccaccgg tatcggtgga ggcgatcggc agctcctggt gcagcagcac cgggccggta tccaggccag cctccatctg catcaggcac accccggtct tcgcgtcacc agcctggatc gcacgctgga tcggcgcagc gccgcgccag cgcggcagca gcgaggcatg cacgttccag caaccatggg tcgggatggc cagtactgcc ttgggcagga tcaggccata ggccaccacc accatcaggt ccggctgcag gtcgcgcaac tgctgctggg cggccgcgtc cttcagcgac tccggctggt agaccggaat gccacgggcc acggcttcga gcttgaccgg cgaaggcgcc aggccacggc ctcggccggc cgggcgatca ggctgggtat agacggccac gacctcatgg tggcgcgcag ccgcgcgcag cgacgacacc atatggtggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMS SLRAAARHHE VVAVYTQPDR PAGRGRGLAP SPVKLEAVAR GIPVYQPESL KDAAAQQQLR DLQPDLMVVV AYGLILPKAV LAIPTHGCWN VHASLLPRWR GAAPIQRAIQ AGDAKTGVCL MQMEAGLDTG PVLLHQELPI ASTDTGGQLH DKLAELGAQV LSDGLGLLRA GIKPIARPQP EQGVTYAHKL DKAEARLDWA QDADALARTV RAFNPWPIAE ATLAGERVRI HGAVALEADH GQAPGTVLAA GRDGIDIACG QGALRLRVLQ REGGKAITAA DYLNARRDLR VGA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgtcgt cgctgcgcgc ggctgcgcgc caccatgagg tcgtggccgt ctatacccag cctgatcgcc cggccggccg aggccgtggc ctggcgcctt cgccggtcaa gctcgaagcc gtggcccgtg gcattccggt ctaccagccg gagtcgctga aggacgcggc cgcccagcag cagttgcgcg acctgcagcc ggacctgatg gtggtggtgg cctatggcct gatcctgccc aaggcagtac tggccatccc gacccatggt tgctggaacg tgcatgcctc gctgctgccg cgctggcgcg gcgctgcgcc gatccagcgt gcgatccagg ctggtgacgc gaagaccggg gtgtgcctga tgcagatgga ggctggcctg gataccggcc cggtgctgct gcaccaggag ctgccgatcg cctccaccga taccggtggc cagctgcacg acaagctggc cgagcttggt gcccaggtgt tgtccgatgg cctgggcctg ctgcgcgcgg gcatcaagcc gatcgcgcga ccgcagccgg aacagggcgt gacgtacgcg cacaagctgg acaaggccga agccaggctg gactgggcgc aggacgccga cgcgctggcg cgtaccgtgc gcgcgttcaa tccgtggccg atcgccgagg caacgctggc tggcgaacgc gtgcgcatcc atggtgcggt ggcactggag gcagaccacg gccaggcgcc gggcaccgtg ctggccgccg gccgcgacgg catcgacatt gcctgtggcc agggcgcgct gcgcctgcgc gtgctgcagc gtgaaggcgg caaggcgatc accgctgccg attacctcaa cgctcgccgc gacctgcgcg tgggggcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat