StmaA.01029.a

ATP-dependent hsl protease ATP-binding subunit HslU (hslU)

CENTER ID: StmaA.01029.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01029.a.B1.GE43282 Full length(StmaA.01029.a) 1 457
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.01029.a.B1.PW38723 Full length(StmaA.01029.a) 1 457

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3846

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSKIEVSSAT MTPREIVQEL DRHIVGQHDA KRAVAIALRN RWRRMQLVPE LRNEVMPKNI LMIGPTGVGK TEIARRLATL ANAPFVKVEA TRFTEVGYVG KDVEQIIRDL ADTAVKLYRE QAKVRVRTQA EERAEDRILD ALLPRRSGGI GFDPEVARNE PSAQDNETRI KFRKMLRNGE LDEREIELDL AANVSMDIMT PPGMEEMGQQ LKSMFANLGG GAKAHKRTLT IKAARPLLIE EEAGKLVNED DIRTAAIEAC EQHGIVFIDE IDKVAKRGDN VGGGDVSREG VQRDLLPLVE GSNVSTKYGT IKTDHILFIA SGAFHLAKPS DLIPELQGRF PIRVELGALS KGDFVRILTE PKAALTKQYE ALLATEGVKV SFTADAIERL AEIAFQVNER QENIGARRLH TVLERLLDSL SYEAPDRDGE SLAIDSAYVD KHLGELVQDP DLSRYIL
NT Sequence
ATGTCGAAGA TCGAAGTTTC CTCCGCCACC ATGACCCCGC GCGAGATCGT GCAGGAACTG GACCGGCATA TCGTGGGCCA GCACGACGCC AAGCGCGCCG TGGCCATCGC CCTGCGCAAC CGCTGGCGCC GCATGCAGCT GGTGCCTGAG CTGCGCAATG AAGTGATGCC GAAGAACATC CTGATGATCG GCCCGACCGG CGTCGGCAAG ACCGAGATCG CGCGTCGCCT GGCCACGCTG GCCAACGCGC CGTTCGTGAA GGTCGAAGCC ACCCGCTTCA CCGAAGTCGG CTACGTCGGC AAGGACGTCG AGCAGATCAT CCGCGACCTG GCCGATACCG CAGTGAAGCT GTACCGCGAA CAGGCCAAGG TGCGCGTGCG TACCCAGGCC GAGGAACGCG CCGAAGACCG CATCCTCGAT GCGCTGCTGC CGCGCCGCAG CGGTGGCATC GGCTTCGATC CGGAAGTCGC CCGCAACGAA CCGTCGGCGC AGGACAACGA GACCCGCATC AAGTTCCGCA AGATGCTGCG CAACGGTGAG CTGGACGAGC GCGAGATCGA ACTCGACCTG GCCGCCAATG TCAGCATGGA CATCATGACC CCGCCGGGCA TGGAGGAAAT GGGCCAGCAG CTGAAGTCGA TGTTCGCCAA CCTTGGCGGC GGCGCGAAGG CGCACAAGCG CACGCTGACC ATCAAGGCCG CGCGACCGCT GCTGATCGAG GAAGAGGCCG GCAAGCTGGT CAACGAGGAC GATATCCGCA CTGCGGCGAT CGAGGCCTGC GAGCAGCACG GCATCGTCTT CATCGACGAG ATCGACAAGG TCGCCAAGCG TGGCGATAAC GTGGGCGGCG GCGATGTCTC CCGCGAAGGC GTGCAGCGCG ACCTGCTGCC GCTGGTGGAA GGCTCCAACG TGTCCACCAA GTACGGCACG ATCAAGACCG ACCACATCCT GTTCATTGCA TCCGGTGCGT TCCACCTGGC CAAGCCCAGC GATCTGATTC CGGAGCTGCA GGGTCGCTTC CCGATCCGCG TTGAACTGGG TGCGCTGAGC AAGGGTGATT TCGTGCGCAT CCTCACCGAG CCGAAGGCCG CGCTGACCAA GCAGTACGAG GCGCTGCTGG CTACCGAGGG CGTCAAGGTC AGCTTCACCG CCGACGCCAT CGAGCGCCTG GCCGAAATCG CCTTCCAGGT GAACGAGCGC CAGGAGAACA TCGGTGCCCG TCGCCTGCAT ACGGTGCTGG AGCGCCTGCT GGATTCGCTC AGCTACGAGG CACCGGACCG CGATGGCGAA AGCCTGGCGA TCGACAGCGC CTACGTCGAC AAGCACCTGG GTGAGCTGGT GCAGGACCCG GACCTGAGCC GCTACATCCT G
Details for StmaA.01029.a.B1.GE43282
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 52kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS KIEVSSATMT PREIVQELDR HIVGQHDAKR AVAIALRNRW RRMQLVPELR NEVMPKNILM IGPTGVGKTE IARRLATLAN APFVKVEATR FTEVGYVGKD VEQIIRDLAD TAVKLYREQA KVRVRTQAEE RAEDRILDAL LPRRSGGIGF DPEVARNEPS AQDNETRIKF RKMLRNGELD EREIELDLAA NVSMDIMTPP GMEEMGQQLK SMFANLGGGA KAHKRTLTIK AARPLLIEEE AGKLVNEDDI RTAAIEACEQ HGIVFIDEID KVAKRGDNVG GGDVSREGVQ RDLLPLVEGS NVSTKYGTIK TDHILFIASG AFHLAKPSDL IPELQGRFPI RVELGALSKG DFVRILTEPK AALTKQYEAL LATEGVKVSF TADAIERLAE IAFQVNERQE NIGARRLHTV LERLLDSLSY EAPDRDGESL AIDSAYVDKH LGELVQ
Validated NT Sequence
tcctgcacca gctcacccag gtgcttgtcg acgtaggcgc tgtcgatcgc caggctttcg ccatcgcggt ccggtgcctc gtagctgagc gaatccagca ggcgctccag caccgtatgc aggcgacggg caccgatgtt ctcctggcgc tcgttcacct ggaaggcgat ttcggccagg cgctcgatgg cgtcggcggt gaagctgacc ttgacgccct cggtagccag cagcgcctcg tactgcttgg tcagcgcggc cttcggctcg gtgaggatgc gcacgaaatc acccttgctc agcgcaccca gttcaacgcg gatcgggaag cgaccctgca gctccggaat cagatcgctg ggcttggcca ggtggaacgc accggatgca atgaacagga tgtggtcggt cttgatcgtg ccgtacttgg tggacacgtt ggagccttcc accagcggca gcaggtcgcg ctgcacgcct tcgcgggaga catcgccgcc gcccacgtta tcgccacgct tggcgacctt gtcgatctcg tcgatgaaga cgatgccgtg ctgctcgcag gcctcgatcg ccgcagtgcg gatatcgtcc tcgttgacca gcttgccggc ctcttcctcg atcagcagcg gtcgcgcggc cttgatggtc agcgtgcgct tgtgcgcctt cgcgccgccg ccaaggttgg cgaacatcga cttcagctgc tggcccattt cctccatgcc cggcggggtc atgatgtcca tgctgacatt ggcggccagg tcgagttcga tctcgcgctc gtccagctca ccgttgcgca gcatcttgcg gaacttgatg cgggtctcgt tgtcctgcgc cgacggttcg ttgcgggcga cttccggatc gaagccgatg ccaccgctgc ggcgcggcag cagcgcatcg aggatgcggt cttcggcgcg ttcctcggcc tgggtacgca cgcgcacctt ggcctgttcg cggtacagct tcactgcggt atcggccagg tcgcggatga tctgctcgac gtccttgccg acgtagccga cttcggtgaa gcgggtggct tcgaccttca cgaacggcgc gttggccagc gtggccaggc gacgcgcgat ctcggtcttg ccgacgccgg tcgggccgat catcaggatg ttcttcggca tcacttcatt gcgcagctca ggcaccagct gcatgcggcg ccagcggttg cgcagggcga tggccacggc gcgcttggcg tcgtgctggc ccacgatatg ccggtccagt tcctgcacga tctcgcgcgg ggtcatggtg gcggaggaaa cttcgatctt cgacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMS KIEVSSATMT PREIVQELDR HIVGQHDAKR AVAIALRNRW RRMQLVPELR NEVMPKNILM IGPTGVGKTE IARRLATLAN APFVKVEATR FTEVGYVGKD VEQIIRDLAD TAVKLYREQA KVRVRTQAEE RAEDRILDAL LPRRSGGIGF DPEVARNEPS AQDNETRIKF RKMLRNGELD EREIELDLAA NVSMDIMTPP GMEEMGQQLK SMFANLGGGA KAHKRTLTIK AARPLLIEEE AGKLVNEDDI RTAAIEACEQ HGIVFIDEID KVAKRGDNVG GGDVSREGVQ RDLLPLVEGS NVSTKYGTIK TDHILFIASG AFHLAKPSDL IPELQGRFPI RVELGALSKG DFVRILTEPK AALTKQYEAL LATEGVKVSF TADAIERLAE IAFQVNERQE NIGARRLHTV LERLLDSLSY EAPDRDGESL AIDSAYVDKH LGELVQDPDL SRYIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga agatcgaagt ttcctccgcc accatgaccc cgcgcgagat cgtgcaggaa ctggaccggc atatcgtggg ccagcacgac gccaagcgcg ccgtggccat cgccctgcgc aaccgctggc gccgcatgca gctggtgcct gagctgcgca atgaagtgat gccgaagaac atcctgatga tcggcccgac cggcgtcggc aagaccgaga tcgcgcgtcg cctggccacg ctggccaacg cgccgttcgt gaaggtcgaa gccacccgct tcaccgaagt cggctacgtc ggcaaggacg tcgagcagat catccgcgac ctggccgata ccgcagtgaa gctgtaccgc gaacaggcca aggtgcgcgt gcgtacccag gccgaggaac gcgccgaaga ccgcatcctc gatgcgctgc tgccgcgccg cagcggtggc atcggcttcg atccggaagt cgcccgcaac gaaccgtcgg cgcaggacaa cgagacccgc atcaagttcc gcaagatgct gcgcaacggt gagctggacg agcgcgagat cgaactcgac ctggccgcca atgtcagcat ggacatcatg accccgccgg gcatggagga aatgggccag cagctgaagt cgatgttcgc caaccttggc ggcggcgcga aggcgcacaa gcgcacgctg accatcaagg ccgcgcgacc gctgctgatc gaggaagagg ccggcaagct ggtcaacgag gacgatatcc gcactgcggc gatcgaggcc tgcgagcagc acggcatcgt cttcatcgac gagatcgaca aggtcgccaa gcgtggcgat aacgtgggcg gcggcgatgt ctcccgcgaa ggcgtgcagc gcgacctgct gccgctggtg gaaggctcca acgtgtccac caagtacggc acgatcaaga ccgaccacat cctgttcatt gcatccggtg cgttccacct ggccaagccc agcgatctga ttccggagct gcagggtcgc ttcccgatcc gcgttgaact gggtgcgctg agcaagggtg atttcgtgcg catcctcacc gagccgaagg ccgcgctgac caagcagtac gaggcgctgc tggctaccga gggcgtcaag gtcagcttca ccgccgacgc catcgagcgc ctggccgaaa tcgccttcca ggtgaacgag cgccaggaga acatcggtgc ccgtcgcctg catacggtgc tggagcgcct gctggattcg ctcagctacg aggcaccgga ccgcgatggc gaaagcctgg cgatcgacag cgcctacgtc gacaagcacc tgggtgagct ggtgcaggac ccggacctga gccgctacat cctgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for StmaA.01029.a.B1.PW38723
PURIFICATION DATe: 10/17/2019
CONCENTRATION: 14mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS KIEVSSATMT PREIVQELDR HIVGQHDAKR AVAIALRNRW RRMQLVPELR NEVMPKNILM IGPTGVGKTE IARRLATLAN APFVKVEATR FTEVGYVGKD VEQIIRDLAD TAVKLYREQA KVRVRTQAEE RAEDRILDAL LPRRSGGIGF DPEVARNEPS AQDNETRIKF RKMLRNGELD EREIELDLAA NVSMDIMTPP GMEEMGQQLK SMFANLGGGA KAHKRTLTIK AARPLLIEEE AGKLVNEDDI RTAAIEACEQ HGIVFIDEID KVAKRGDNVG GGDVSREGVQ RDLLPLVEGS NVSTKYGTIK TDHILFIASG AFHLAKPSDL IPELQGRFPI RVELGALSKG DFVRILTEPK AALTKQYEAL LATEGVKVSF TADAIERLAE IAFQVNERQE NIGARRLHTV LERLLDSLSY EAPDRDGESL AIDSAYVDKH LGELVQ
Validated NT Sequence
tcctgcacca gctcacccag gtgcttgtcg acgtaggcgc tgtcgatcgc caggctttcg ccatcgcggt ccggtgcctc gtagctgagc gaatccagca ggcgctccag caccgtatgc aggcgacggg caccgatgtt ctcctggcgc tcgttcacct ggaaggcgat ttcggccagg cgctcgatgg cgtcggcggt gaagctgacc ttgacgccct cggtagccag cagcgcctcg tactgcttgg tcagcgcggc cttcggctcg gtgaggatgc gcacgaaatc acccttgctc agcgcaccca gttcaacgcg gatcgggaag cgaccctgca gctccggaat cagatcgctg ggcttggcca ggtggaacgc accggatgca atgaacagga tgtggtcggt cttgatcgtg ccgtacttgg tggacacgtt ggagccttcc accagcggca gcaggtcgcg ctgcacgcct tcgcgggaga catcgccgcc gcccacgtta tcgccacgct tggcgacctt gtcgatctcg tcgatgaaga cgatgccgtg ctgctcgcag gcctcgatcg ccgcagtgcg gatatcgtcc tcgttgacca gcttgccggc ctcttcctcg atcagcagcg gtcgcgcggc cttgatggtc agcgtgcgct tgtgcgcctt cgcgccgccg ccaaggttgg cgaacatcga cttcagctgc tggcccattt cctccatgcc cggcggggtc atgatgtcca tgctgacatt ggcggccagg tcgagttcga tctcgcgctc gtccagctca ccgttgcgca gcatcttgcg gaacttgatg cgggtctcgt tgtcctgcgc cgacggttcg ttgcgggcga cttccggatc gaagccgatg ccaccgctgc ggcgcggcag cagcgcatcg aggatgcggt cttcggcgcg ttcctcggcc tgggtacgca cgcgcacctt ggcctgttcg cggtacagct tcactgcggt atcggccagg tcgcggatga tctgctcgac gtccttgccg acgtagccga cttcggtgaa gcgggtggct tcgaccttca cgaacggcgc gttggccagc gtggccaggc gacgcgcgat ctcggtcttg ccgacgccgg tcgggccgat catcaggatg ttcttcggca tcacttcatt gcgcagctca ggcaccagct gcatgcggcg ccagcggttg cgcagggcga tggccacggc gcgcttggcg tcgtgctggc ccacgatatg ccggtccagt tcctgcacga tctcgcgcgg ggtcatggtg gcggaggaaa cttcgatctt cgacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMS KIEVSSATMT PREIVQELDR HIVGQHDAKR AVAIALRNRW RRMQLVPELR NEVMPKNILM IGPTGVGKTE IARRLATLAN APFVKVEATR FTEVGYVGKD VEQIIRDLAD TAVKLYREQA KVRVRTQAEE RAEDRILDAL LPRRSGGIGF DPEVARNEPS AQDNETRIKF RKMLRNGELD EREIELDLAA NVSMDIMTPP GMEEMGQQLK SMFANLGGGA KAHKRTLTIK AARPLLIEEE AGKLVNEDDI RTAAIEACEQ HGIVFIDEID KVAKRGDNVG GGDVSREGVQ RDLLPLVEGS NVSTKYGTIK TDHILFIASG AFHLAKPSDL IPELQGRFPI RVELGALSKG DFVRILTEPK AALTKQYEAL LATEGVKVSF TADAIERLAE IAFQVNERQE NIGARRLHTV LERLLDSLSY EAPDRDGESL AIDSAYVDKH LGELVQDPDL SRYIL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGA AGATCGAAGT TTCCTCCGCC ACCATGACCC CGCGCGAGAT CGTGCAGGAA CTGGACCGGC ATATCGTGGG CCAGCACGAC GCCAAGCGCG CCGTGGCCAT CGCCCTGCGC AACCGCTGGC GCCGCATGCA GCTGGTGCCT GAGCTGCGCA ATGAAGTGAT GCCGAAGAAC ATCCTGATGA TCGGCCCGAC CGGCGTCGGC AAGACCGAGA TCGCGCGTCG CCTGGCCACG CTGGCCAACG CGCCGTTCGT GAAGGTCGAA GCCACCCGCT TCACCGAAGT CGGCTACGTC GGCAAGGACG TCGAGCAGAT CATCCGCGAC CTGGCCGATA CCGCAGTGAA GCTGTACCGC GAACAGGCCA AGGTGCGCGT GCGTACCCAG GCCGAGGAAC GCGCCGAAGA CCGCATCCTC GATGCGCTGC TGCCGCGCCG CAGCGGTGGC ATCGGCTTCG ATCCGGAAGT CGCCCGCAAC GAACCGTCGG CGCAGGACAA CGAGACCCGC ATCAAGTTCC GCAAGATGCT GCGCAACGGT GAGCTGGACG AGCGCGAGAT CGAACTCGAC CTGGCCGCCA ATGTCAGCAT GGACATCATG ACCCCGCCGG GCATGGAGGA AATGGGCCAG CAGCTGAAGT CGATGTTCGC CAACCTTGGC GGCGGCGCGA AGGCGCACAA GCGCACGCTG ACCATCAAGG CCGCGCGACC GCTGCTGATC GAGGAAGAGG CCGGCAAGCT GGTCAACGAG GACGATATCC GCACTGCGGC GATCGAGGCC TGCGAGCAGC ACGGCATCGT CTTCATCGAC GAGATCGACA AGGTCGCCAA GCGTGGCGAT AACGTGGGCG GCGGCGATGT CTCCCGCGAA GGCGTGCAGC GCGACCTGCT GCCGCTGGTG GAAGGCTCCA ACGTGTCCAC CAAGTACGGC ACGATCAAGA CCGACCACAT CCTGTTCATT GCATCCGGTG CGTTCCACCT GGCCAAGCCC AGCGATCTGA TTCCGGAGCT GCAGGGTCGC TTCCCGATCC GCGTTGAACT GGGTGCGCTG AGCAAGGGTG ATTTCGTGCG CATCCTCACC GAGCCGAAGG CCGCGCTGAC CAAGCAGTAC GAGGCGCTGC TGGCTACCGA GGGCGTCAAG GTCAGCTTCA CCGCCGACGC CATCGAGCGC CTGGCCGAAA TCGCCTTCCA GGTGAACGAG CGCCAGGAGA ACATCGGTGC CCGTCGCCTG CATACGGTGC TGGAGCGCCT GCTGGATTCG CTCAGCTACG AGGCACCGGA CCGCGATGGC GAAAGCCTGG CGATCGACAG CGCCTACGTC GACAAGCACC TGGGTGAGCT GGTGCAGGAC CCGGACCTGA GCCGCTACAT CCTGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT