StmaA.01026.c
Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) (hemL)
| CENTER ID: | StmaA.01026.c |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01026.c.B1.GE43281 | Full length(StmaA.01026.c) | 1 | 429 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01026.c.B1.PW38743 | Full length(StmaA.01026.c) | 1 | 429 |
Structures
6W80
DEPOSITED: 3/20/2020
DETERMINATION: XRay
CLONE: StmaA.01026.c.B1.GE43281
PROTEIN: StmaA.01026.c.B1.PW38743
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3648 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNHDQSHALF SRAQQLLPGG VNSPVRAFKS VGGEPFFVER ADGAYLYDVD GNRYIDYVGS WGPMIVGHNH PAVRQAVKKA IDNGLSFGAP CAGEVTMAET ITRLVPSCEM VRMVNSGTEA TLSAIRLARG ATGRNRIVKF EGCYHGHGDS FLVKAGSGML TLGVPTSPGV PAGLSELTLT LPYNDFEAAT ALFEQQGDDI AGLIIEPVVG NANCIPPREG YLQHLRALCT KHGALLIFDE VMTGFRVALG GAQAHYGITP DLTTFGKIIG GGMPVGAYGG RRELMQQIAP AGPIYQAGTL SGNPVAMAAG LAMLELVQQP GFHADLAERT ARLCAGLEAA AADAGVAVTT TRVGAMFGLF FTSEKVETYA QATACDIPAF NRFFHAMLEQ GVFLAPSAYE AGFLSSAHDD AVIEATLAAA RVAFRAAKG
NT Sequence
ATGAACCACG ACCAGTCCCA CGCCCTGTTC TCCCGTGCCC AGCAGCTGCT GCCGGGCGGC GTCAATTCGC CGGTACGTGC GTTCAAGTCG GTCGGCGGCG AGCCGTTCTT CGTTGAACGC GCCGATGGCG CCTACCTGTA CGACGTCGAT GGCAACCGCT ACATCGACTA CGTCGGTTCC TGGGGCCCGA TGATCGTCGG CCACAACCAC CCGGCCGTGC GCCAGGCGGT GAAGAAGGCG ATCGACAATG GCCTGTCCTT CGGTGCGCCG TGCGCGGGAG AAGTCACCAT GGCCGAGACC ATCACCCGCC TGGTGCCATC GTGCGAGATG GTGCGCATGG TCAACTCCGG CACCGAGGCC ACGCTGTCGG CGATCCGCCT GGCGCGTGGC GCCACCGGCC GCAACCGCAT CGTCAAGTTC GAAGGCTGCT ACCACGGCCA CGGCGACTCG TTCCTGGTCA AGGCCGGCAG CGGCATGCTG ACCCTGGGCG TGCCAACCTC GCCGGGCGTC CCGGCCGGCC TGAGCGAACT GACCCTGACC CTGCCCTACA ACGACTTCGA AGCGGCCACC GCCCTGTTCG AACAGCAGGG TGACGACATC GCCGGCCTGA TCATCGAACC GGTGGTCGGC AACGCCAACT GCATCCCGCC GCGCGAGGGC TACCTGCAAC ACCTGCGTGC GCTGTGCACG AAGCATGGCG CGCTGCTGAT CTTCGACGAA GTGATGACCG GCTTCCGTGT TGCCCTTGGC GGTGCGCAGG CGCATTACGG CATCACCCCG GACCTGACCA CTTTCGGCAA GATCATCGGC GGTGGCATGC CGGTGGGTGC CTACGGTGGT CGCCGCGAGC TGATGCAGCA GATCGCACCG GCCGGCCCGA TCTACCAGGC CGGCACGCTG AGCGGCAATC CGGTGGCAAT GGCTGCCGGC CTGGCGATGC TGGAGCTGGT CCAGCAGCCG GGTTTCCATG CCGACCTGGC CGAGCGCACC GCACGCCTGT GTGCGGGTCT GGAAGCGGCC GCCGCCGACG CCGGTGTGGC CGTGACCACC ACCCGCGTGG GTGCGATGTT CGGGCTGTTC TTCACCAGCG AGAAGGTGGA GACCTATGCG CAGGCCACCG CCTGTGACAT TCCGGCGTTC AACCGCTTCT TCCACGCGAT GCTGGAGCAG GGCGTGTTCC TGGCCCCGTC GGCGTACGAG GCCGGCTTCC TGTCCAGCGC CCACGACGAT GCCGTGATCG AGGCGACGCT GGCCGCAGCA CGCGTGGCGT TCAGGGCCGC CAAGGGC
Details for StmaA.01026.c.B1.GE43281
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 46kDa |
| OBSERVED MW: | 47kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMN HDQSHALFSR AQQLLPGGVN SPVRAFKSVG GEPFFVERAD GAYLYDVDGN RYIDYVGSWG PMIVGHNHPA VRQAVKKAID NGLSFGAPCA GEVTMAETIT RLVPSCEMVR MVNSGTEATL SAIRLARGAT GRNRIVKFEG CYHGHGDSFL VKAGSGMLTL GVPTSPGVPA GLSELTLTLP YNDFEAATAL FEQQGDDIAG LIIEPVVGNA NCIPPREGYL QHLRALCTKH GALLIFDEVM TGFRVALGGA QAHYGITPDL TTFGKIIGGG MPVGAYGGRR ELMQQIAPAG PIYQAGTLSG NPVAMAAGLA MLELVQQPGF HADLAERTAR LCAGLEAAAA DAGVAVTTTR VGAMFGLFFT SEKVETYAQA TACDIPAFNR FFHAMLEQGV FLAPSAYEAG FLSSAHDDAV IEATLAAA
Validated NT Sequence
gngctgcggc cagcgtcgcc tcgatcacgg catcgtcgtg ggcgctggac aggaagccgg cctcgtacgc cgacggggcc aggaacacgc cctgctccag catcgcgtgg aagaagcggt tgaacgccgg aatgtcacag gcggtggcct gcgcataggt ctccaccttc tcgctggtga agaacagccc gaacatcgca cccacgcggg tggtggtcac ggccacaccg gcgtcggcgg cggccgcttc cagacccgca cacaggcgtg cggtgcgctc ggccaggtcg gcatggaaac ccggctgctg gaccagctcc agcatcgcca ggccggcagc cattgccacc ggattgccgc tcagcgtgcc ggcctggtag atcgggccgg ccggtgcgat ctgctgcatc agctcgcggc gaccaccgta ggcacccacc ggcatgccac cgccgatgat cttgccgaaa gtggtcaggt ccggggtgat gccgtaatgc gcctgcgcac cgccaagggc aacacggaag ccggtcatca cttcgtcgaa gatcagcagc gcgccatgct tcgtgcacag cgcacgcagg tgttgcaggt agccctcgcg cggcgggatg cagttggcgt tgccgaccac cggttcgatg atcaggccgg cgatgtcgtc accctgctgt tcgaacaggg cggtggccgc ttcgaagtcg ttgtagggca gggtcagggt cagttcgctc aggccggccg ggacgcccgg cgaggttggc acgcccaggg tcagcatgcc gctgccggcc ttgaccagga acgagtcgcc gtggccgtgg tagcagcctt cgaacttgac gatgcggttg cggccggtgg cgccacgcgc caggcggatc gccgacagcg tggcctcggt gccggagttg accatgcgca ccatctcgca cgatggcacc aggcgggtga tggtctcggc catggtgact tctcccgcgc acggcgcacc gaaggacagg ccattgtcga tcgccttctt caccgcctgg cgcacggccg ggtggttgtg gccgacgatc atcgggcccc aggaaccgac gtagtcgatg tagcggttgc catcgacgtc gtacaggtag gcgccatcgg cgcgttcaac gaagaacggc tcgccgccga ccgacttgaa cgcacgtacc ggcgaattga cgccgcccgg cagcagctgc tgggcacggg agaacagggc gtgggactgg tcgtggttca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMN HDQSHALFSR AQQLLPGGVN SPVRAFKSVG GEPFFVERAD GAYLYDVDGN RYIDYVGSWG PMIVGHNHPA VRQAVKKAID NGLSFGAPCA GEVTMAETIT RLVPSCEMVR MVNSGTEATL SAIRLARGAT GRNRIVKFEG CYHGHGDSFL VKAGSGMLTL GVPTSPGVPA GLSELTLTLP YNDFEAATAL FEQQGDDIAG LIIEPVVGNA NCIPPREGYL QHLRALCTKH GALLIFDEVM TGFRVALGGA QAHYGITPDL TTFGKIIGGG MPVGAYGGRR ELMQQIAPAG PIYQAGTLSG NPVAMAAGLA MLELVQQPGF HADLAERTAR LCAGLEAAAA DAGVAVTTTR VGAMFGLFFT SEKVETYAQA TACDIPAFNR FFHAMLEQGV FLAPSAYEAG FLSSAHDDAV IEATLAAARV AFRAAKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacc acgaccagtc ccacgccctg ttctcccgtg cccagcagct gctgccgggc ggcgtcaatt cgccggtacg tgcgttcaag tcggtcggcg gcgagccgtt cttcgttgaa cgcgccgatg gcgcctacct gtacgacgtc gatggcaacc gctacatcga ctacgtcggt tcctggggcc cgatgatcgt cggccacaac cacccggccg tgcgccaggc ggtgaagaag gcgatcgaca atggcctgtc cttcggtgcg ccgtgcgcgg gagaagtcac catggccgag accatcaccc gcctggtgcc atcgtgcgag atggtgcgca tggtcaactc cggcaccgag gccacgctgt cggcgatccg cctggcgcgt ggcgccaccg gccgcaaccg catcgtcaag ttcgaaggct gctaccacgg ccacggcgac tcgttcctgg tcaaggccgg cagcggcatg ctgaccctgg gcgtgccaac ctcgccgggc gtcccggccg gcctgagcga actgaccctg accctgccct acaacgactt cgaagcggcc accgccctgt tcgaacagca gggtgacgac atcgccggcc tgatcatcga accggtggtc ggcaacgcca actgcatccc gccgcgcgag ggctacctgc aacacctgcg tgcgctgtgc acgaagcatg gcgcgctgct gatcttcgac gaagtgatga ccggcttccg tgttgccctt ggcggtgcgc aggcgcatta cggcatcacc ccggacctga ccactttcgg caagatcatc ggcggtggca tgccggtggg tgcctacggt ggtcgccgcg agctgatgca gcagatcgca ccggccggcc cgatctacca ggccggcacg ctgagcggca atccggtggc aatggctgcc ggcctggcga tgctggagct ggtccagcag ccgggtttcc atgccgacct ggccgagcgc accgcacgcc tgtgtgcggg tctggaagcg gccgccgccg acgccggtgt ggccgtgacc accacccgcg tgggtgcgat gttcgggctg ttcttcacca gcgagaaggt ggagacctat gcgcaggcca ccgcctgtga cattccggcg ttcaaccgct tcttccacgc gatgctggag cagggcgtgt tcctggcccc gtcggcgtac gaggccggct tcctgtccag cgcccacgac gatgccgtga tcgaggcgac gctggccgca gcacgcgtgg cgttcagggc cgccaagggc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for StmaA.01026.c.B1.PW38743
| PURIFICATION DATe: | 10/24/2019 |
| CONCENTRATION: | 13.4mg/ml |
| OBSERVED MW: | 46kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN HDQSHALFSR AQQLLPGGVN SPVRAFKSVG GEPFFVERAD GAYLYDVDGN RYIDYVGSWG PMIVGHNHPA VRQAVKKAID NGLSFGAPCA GEVTMAETIT RLVPSCEMVR MVNSGTEATL SAIRLARGAT GRNRIVKFEG CYHGHGDSFL VKAGSGMLTL GVPTSPGVPA GLSELTLTLP YNDFEAATAL FEQQGDDIAG LIIEPVVGNA NCIPPREGYL QHLRALCTKH GALLIFDEVM TGFRVALGGA QAHYGITPDL TTFGKIIGGG MPVGAYGGRR ELMQQIAPAG PIYQAGTLSG NPVAMAAGLA MLELVQQPGF HADLAERTAR LCAGLEAAAA DAGVAVTTTR VGAMFGLFFT SEKVETYAQA TACDIPAFNR FFHAMLEQGV FLAPSAYEAG FLSSAHDDAV IEATLAAA
Validated NT Sequence
gngctgcggc cagcgtcgcc tcgatcacgg catcgtcgtg ggcgctggac aggaagccgg cctcgtacgc cgacggggcc aggaacacgc cctgctccag catcgcgtgg aagaagcggt tgaacgccgg aatgtcacag gcggtggcct gcgcataggt ctccaccttc tcgctggtga agaacagccc gaacatcgca cccacgcggg tggtggtcac ggccacaccg gcgtcggcgg cggccgcttc cagacccgca cacaggcgtg cggtgcgctc ggccaggtcg gcatggaaac ccggctgctg gaccagctcc agcatcgcca ggccggcagc cattgccacc ggattgccgc tcagcgtgcc ggcctggtag atcgggccgg ccggtgcgat ctgctgcatc agctcgcggc gaccaccgta ggcacccacc ggcatgccac cgccgatgat cttgccgaaa gtggtcaggt ccggggtgat gccgtaatgc gcctgcgcac cgccaagggc aacacggaag ccggtcatca cttcgtcgaa gatcagcagc gcgccatgct tcgtgcacag cgcacgcagg tgttgcaggt agccctcgcg cggcgggatg cagttggcgt tgccgaccac cggttcgatg atcaggccgg cgatgtcgtc accctgctgt tcgaacaggg cggtggccgc ttcgaagtcg ttgtagggca gggtcagggt cagttcgctc aggccggccg ggacgcccgg cgaggttggc acgcccaggg tcagcatgcc gctgccggcc ttgaccagga acgagtcgcc gtggccgtgg tagcagcctt cgaacttgac gatgcggttg cggccggtgg cgccacgcgc caggcggatc gccgacagcg tggcctcggt gccggagttg accatgcgca ccatctcgca cgatggcacc aggcgggtga tggtctcggc catggtgact tctcccgcgc acggcgcacc gaaggacagg ccattgtcga tcgccttctt caccgcctgg cgcacggccg ggtggttgtg gccgacgatc atcgggcccc aggaaccgac gtagtcgatg tagcggttgc catcgacgtc gtacaggtag gcgccatcgg cgcgttcaac gaagaacggc tcgccgccga ccgacttgaa cgcacgtacc ggcgaattga cgccgcccgg cagcagctgc tgggcacggg agaacagggc gtgggactgg tcgtggttca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMN HDQSHALFSR AQQLLPGGVN SPVRAFKSVG GEPFFVERAD GAYLYDVDGN RYIDYVGSWG PMIVGHNHPA VRQAVKKAID NGLSFGAPCA GEVTMAETIT RLVPSCEMVR MVNSGTEATL SAIRLARGAT GRNRIVKFEG CYHGHGDSFL VKAGSGMLTL GVPTSPGVPA GLSELTLTLP YNDFEAATAL FEQQGDDIAG LIIEPVVGNA NCIPPREGYL QHLRALCTKH GALLIFDEVM TGFRVALGGA QAHYGITPDL TTFGKIIGGG MPVGAYGGRR ELMQQIAPAG PIYQAGTLSG NPVAMAAGLA MLELVQQPGF HADLAERTAR LCAGLEAAAA DAGVAVTTTR VGAMFGLFFT SEKVETYAQA TACDIPAFNR FFHAMLEQGV FLAPSAYEAG FLSSAHDDAV IEATLAAARV AFRAAKG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACC ACGACCAGTC CCACGCCCTG TTCTCCCGTG CCCAGCAGCT GCTGCCGGGC GGCGTCAATT CGCCGGTACG TGCGTTCAAG TCGGTCGGCG GCGAGCCGTT CTTCGTTGAA CGCGCCGATG GCGCCTACCT GTACGACGTC GATGGCAACC GCTACATCGA CTACGTCGGT TCCTGGGGCC CGATGATCGT CGGCCACAAC CACCCGGCCG TGCGCCAGGC GGTGAAGAAG GCGATCGACA ATGGCCTGTC CTTCGGTGCG CCGTGCGCGG GAGAAGTCAC CATGGCCGAG ACCATCACCC GCCTGGTGCC ATCGTGCGAG ATGGTGCGCA TGGTCAACTC CGGCACCGAG GCCACGCTGT CGGCGATCCG CCTGGCGCGT GGCGCCACCG GCCGCAACCG CATCGTCAAG TTCGAAGGCT GCTACCACGG CCACGGCGAC TCGTTCCTGG TCAAGGCCGG CAGCGGCATG CTGACCCTGG GCGTGCCAAC CTCGCCGGGC GTCCCGGCCG GCCTGAGCGA ACTGACCCTG ACCCTGCCCT ACAACGACTT CGAAGCGGCC ACCGCCCTGT TCGAACAGCA GGGTGACGAC ATCGCCGGCC TGATCATCGA ACCGGTGGTC GGCAACGCCA ACTGCATCCC GCCGCGCGAG GGCTACCTGC AACACCTGCG TGCGCTGTGC ACGAAGCATG GCGCGCTGCT GATCTTCGAC GAAGTGATGA CCGGCTTCCG TGTTGCCCTT GGCGGTGCGC AGGCGCATTA CGGCATCACC CCGGACCTGA CCACTTTCGG CAAGATCATC GGCGGTGGCA TGCCGGTGGG TGCCTACGGT GGTCGCCGCG AGCTGATGCA GCAGATCGCA CCGGCCGGCC CGATCTACCA GGCCGGCACG CTGAGCGGCA ATCCGGTGGC AATGGCTGCC GGCCTGGCGA TGCTGGAGCT GGTCCAGCAG CCGGGTTTCC ATGCCGACCT GGCCGAGCGC ACCGCACGCC TGTGTGCGGG TCTGGAAGCG GCCGCCGCCG ACGCCGGTGT GGCCGTGACC ACCACCCGCG TGGGTGCGAT GTTCGGGCTG TTCTTCACCA GCGAGAAGGT GGAGACCTAT GCGCAGGCCA CCGCCTGTGA CATTCCGGCG TTCAACCGCT TCTTCCACGC GATGCTGGAG CAGGGCGTGT TCCTGGCCCC GTCGGCGTAC GAGGCCGGCT TCCTGTCCAG CGCCCACGAC GATGCCGTGA TCGAGGCGAC GCTGGCCGCA GCACGCGTGG CGTTCAGGGC CGCCAAGGGC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT