StmaA.01026.b
N-acetylornithine aminotransferase (EC 2.6.1.11) @ N-succinyl-L,L-diaminopimelate aminotransferase (EC 2.6.1.17) (argD)
| CENTER ID: | StmaA.01026.b |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01026.b.B1.GE43280 | Full length(StmaA.01026.b) | 1 | 408 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01026.b.B1.PW38742 | Full length(StmaA.01026.b) | 1 | 408 |
Structures
6W7X
DEPOSITED: 3/19/2020
DETERMINATION: XRay
CLONE: StmaA.01026.b.B1.GE43280
PROTEIN: StmaA.01026.b.B1.PW38742
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3651 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAATDPLIS LSHYYLPVYK PRQVVLERGQ GARVWDSQGR EFIDLAAGIA VCGLGHNDPD LVAALVEQAG KLWHTSNVFY SAPPLHLAEE LVKASRFAER VFLCNSGAEA NEVAIKMVRK WASSQGRPAD KRVIITFRGS FHGRTMGAVT ATAQPKYQEG YEPLPGGFRY IDFNDEVQLE TAMAAGDVAA VMLEPIQGEG GVMPAKSGFL KRVRELCDQH DALLVLDEIQ AGMGRTGTLF AHWQDDVVPD MVTLAKALGG GFPIGAMLAG PKVAETMQFG AHGTTFGGNP LAAAVARVAL RKLASSEIAA NVSRQSKALR DGFARINEEF GVFSEVRGRG LMLGAVLSKD FAGQAGAILD HAAGQGLLTL QAGPDVLRFV PSLNITDEEV AEGLKRLRAA IAAFIAAR
NT Sequence
ATGACCGCCG CGACCGATCC GCTGATCTCC CTTTCGCACT ATTACCTGCC GGTCTACAAG CCCCGCCAGG TGGTACTGGA GCGAGGCCAG GGCGCCCGCG TATGGGACAG CCAGGGCCGT GAGTTCATCG ACCTGGCTGC CGGCATCGCC GTGTGCGGCC TGGGCCACAA CGATCCGGAC CTGGTGGCCG CGCTGGTCGA GCAGGCCGGC AAGCTCTGGC ACACCAGCAA CGTGTTCTAC AGCGCACCGC CGCTGCACCT GGCCGAGGAA CTGGTGAAGG CCAGTCGCTT CGCCGAGCGG GTGTTCCTGT GCAACTCCGG CGCCGAAGCC AACGAAGTGG CGATCAAGAT GGTCCGCAAG TGGGCCTCCA GCCAGGGCCG CCCGGCCGAC AAGCGCGTCA TCATCACCTT CCGTGGCAGC TTCCACGGCC GCACCATGGG CGCGGTGACC GCTACCGCGC AGCCGAAGTA CCAGGAAGGC TACGAGCCGC TGCCGGGTGG CTTCCGCTAC ATCGATTTCA ACGACGAAGT GCAGCTGGAA ACCGCGATGG CCGCCGGTGA CGTGGCGGCG GTGATGCTGG AGCCGATCCA GGGCGAGGGC GGTGTGATGC CGGCCAAGTC CGGCTTCCTC AAGCGCGTGC GCGAACTGTG CGACCAGCAC GACGCGCTGC TGGTGCTGGA CGAGATCCAG GCCGGCATGG GCCGCACCGG CACGCTGTTC GCGCACTGGC AGGACGACGT GGTGCCGGAC ATGGTCACCC TGGCCAAGGC ACTGGGTGGC GGCTTCCCGA TCGGTGCGAT GCTGGCCGGC CCGAAGGTGG CCGAGACCAT GCAGTTTGGC GCGCACGGCA CCACCTTCGG TGGCAACCCG CTGGCCGCTG CGGTGGCCCG CGTGGCGCTG CGCAAGCTGG CCTCGTCGGA GATCGCCGCC AACGTCAGCC GCCAGTCGAA GGCGCTGCGC GATGGCTTCG CGCGCATCAA CGAAGAGTTC GGCGTGTTCA GCGAGGTGCG TGGTCGCGGC CTGATGCTGG GTGCGGTGCT GAGCAAGGAC TTCGCCGGCC AGGCTGGTGC GATCCTCGAC CACGCTGCCG GGCAGGGGCT GCTGACCCTG CAGGCCGGCC CGGACGTGCT GCGCTTCGTG CCGTCGCTGA ACATCACCGA CGAAGAAGTG GCCGAAGGCC TGAAGCGCCT GCGCGCGGCA ATTGCCGCGT TCATCGCCGC GCGC
Details for StmaA.01026.b.B1.GE43280
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 46kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMT AATDPLISLS HYYLPVYKPR QVVLERGQGA RVWDSQGREF IDLAAGIAVC GLGHNDPDLV AALVEQAGKL WHTSNVFYSA PPLHLAEELV KASRFAERVF LCNSGAEANE VAIKMVRKWA SSQGRPADKR VIITFRGSFH GRTMGAVTAT AQPKYQEGYE PLPGGFRYID FNDEVQLETA MAAGDVAAVM LEPIQGEGGV MPAKSGFLKR VRELCDQHDA LLVLDEIQAG MGRTGTLFAH WQDDVVPDMV TLAKALGGGF PIGAMLAGPK VAETMQFGAH GTTFGGNPLA AAVARVALRK LASSEIAANV SRQSKALRDG FARINEEFGV FSEVRGRGLM LGAVLSKDFA GQAGAILDHA AGQGLLTLQA GPDVLRFVPS LNITDEEVAE GLKRLRA
Validated NT Sequence
gccgcgcgca ggcgcttcag gccttcggcc acttcttcgt cggtgatgtt cagcgacggc acgaagcgca gcacgtccgg gccggcctgc agggtcagca gcccctgccc ggcagcgtgg tcgaggatcg caccagcctg gccggcgaag tccttgctca gcaccgcacc cagcatcagg ccgcgaccac gcacctcgct gaacacgccg aactcttcgt tgatgcgcgc gaagccatcg cgcagcgcct tcgactggcg gctgacgttg gcggcgatct ccgacgaggc cagcttgcgc agcgccacgc gggccaccgc agcggccagc gggttgccac cgaaggtggt gccgtgcgcg ccaaactgca tggtctcggc caccttcggg ccggccagca tcgcaccgat cgggaagccg ccacccagtg ccttggccag ggtgaccatg tccggcacca cgtcgtcctg ccagtgcgcg aacagcgtgc cggtgcggcc catgccggcc tggatctcgt ccagcaccag cagcgcgtcg tgctggtcgc acagttcgcg cacgcgcttg aggaagccgg acttggccgg catcacaccg ccctcgccct ggatcggctc cagcatcacc gccgccacgt caccggcggc catcgcggtt tccagctgca cttcgtcgtt gaaatcgatg tagcggaagc cacccggcag cggctcgtag ccttcctggt acttcggctg cgcggtagcg gtcaccgcgc ccatggtgcg gccgtggaag ctgccacgga aggtgatgat gacgcgcttg tcggccgggc ggccctggct ggaggcccac ttgcggacca tcttgatcgc cacttcgttg gcttcggcgc cggagttgca caggaacacc cgctcggcga agcgactggc cttcaccagt tcctcggcca ggtgcagcgg cggtgcgctg tagaacacgt tgctggtgtg ccagagcttg ccggcctgct cgaccagcgc ggccaccagg tccggatcgt tgtggcccag gccgcacacg gcgatgccgg cagccaggtc gatgaactca cggccctggc tgtcccatac gcgggcgccc tggcctcgct ccagtaccac ctggcggggc ttgtagaccg gcaggtaata gtgcgaaagg gagatcagcg gatcggtcgc ggcggtcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMT AATDPLISLS HYYLPVYKPR QVVLERGQGA RVWDSQGREF IDLAAGIAVC GLGHNDPDLV AALVEQAGKL WHTSNVFYSA PPLHLAEELV KASRFAERVF LCNSGAEANE VAIKMVRKWA SSQGRPADKR VIITFRGSFH GRTMGAVTAT AQPKYQEGYE PLPGGFRYID FNDEVQLETA MAAGDVAAVM LEPIQGEGGV MPAKSGFLKR VRELCDQHDA LLVLDEIQAG MGRTGTLFAH WQDDVVPDMV TLAKALGGGF PIGAMLAGPK VAETMQFGAH GTTFGGNPLA AAVARVALRK LASSEIAANV SRQSKALRDG FARINEEFGV FSEVRGRGLM LGAVLSKDFA GQAGAILDHA AGQGLLTLQA GPDVLRFVPS LNITDEEVAE GLKRLRAAIA AFIAAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg ccgcgaccga tccgctgatc tccctttcgc actattacct gccggtctac aagccccgcc aggtggtact ggagcgaggc cagggcgccc gcgtatggga cagccagggc cgtgagttca tcgacctggc tgccggcatc gccgtgtgcg gcctgggcca caacgatccg gacctggtgg ccgcgctggt cgagcaggcc ggcaagctct ggcacaccag caacgtgttc tacagcgcac cgccgctgca cctggccgag gaactggtga aggccagtcg cttcgccgag cgggtgttcc tgtgcaactc cggcgccgaa gccaacgaag tggcgatcaa gatggtccgc aagtgggcct ccagccaggg ccgcccggcc gacaagcgcg tcatcatcac cttccgtggc agcttccacg gccgcaccat gggcgcggtg accgctaccg cgcagccgaa gtaccaggaa ggctacgagc cgctgccggg tggcttccgc tacatcgatt tcaacgacga agtgcagctg gaaaccgcga tggccgccgg tgacgtggcg gcggtgatgc tggagccgat ccagggcgag ggcggtgtga tgccggccaa gtccggcttc ctcaagcgcg tgcgcgaact gtgcgaccag cacgacgcgc tgctggtgct ggacgagatc caggccggca tgggccgcac cggcacgctg ttcgcgcact ggcaggacga cgtggtgccg gacatggtca ccctggccaa ggcactgggt ggcggcttcc cgatcggtgc gatgctggcc ggcccgaagg tggccgagac catgcagttt ggcgcgcacg gcaccacctt cggtggcaac ccgctggccg ctgcggtggc ccgcgtggcg ctgcgcaagc tggcctcgtc ggagatcgcc gccaacgtca gccgccagtc gaaggcgctg cgcgatggct tcgcgcgcat caacgaagag ttcggcgtgt tcagcgaggt gcgtggtcgc ggcctgatgc tgggtgcggt gctgagcaag gacttcgccg gccaggctgg tgcgatcctc gaccacgctg ccgggcaggg gctgctgacc ctgcaggccg gcccggacgt gctgcgcttc gtgccgtcgc tgaacatcac cgacgaagaa gtggccgaag gcctgaagcg cctgcgcgcg gcaattgccg cgttcatcgc cgcgcgctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for StmaA.01026.b.B1.PW38742
| PURIFICATION DATe: | 10/24/2019 |
| CONCENTRATION: | 29.8mg/ml |
| OBSERVED MW: | 42kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 10 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT AATDPLISLS HYYLPVYKPR QVVLERGQGA RVWDSQGREF IDLAAGIAVC GLGHNDPDLV AALVEQAGKL WHTSNVFYSA PPLHLAEELV KASRFAERVF LCNSGAEANE VAIKMVRKWA SSQGRPADKR VIITFRGSFH GRTMGAVTAT AQPKYQEGYE PLPGGFRYID FNDEVQLETA MAAGDVAAVM LEPIQGEGGV MPAKSGFLKR VRELCDQHDA LLVLDEIQAG MGRTGTLFAH WQDDVVPDMV TLAKALGGGF PIGAMLAGPK VAETMQFGAH GTTFGGNPLA AAVARVALRK LASSEIAANV SRQSKALRDG FARINEEFGV FSEVRGRGLM LGAVLSKDFA GQAGAILDHA AGQGLLTLQA GPDVLRFVPS LNITDEEVAE GLKRLRA
Validated NT Sequence
gccgcgcgca ggcgcttcag gccttcggcc acttcttcgt cggtgatgtt cagcgacggc acgaagcgca gcacgtccgg gccggcctgc agggtcagca gcccctgccc ggcagcgtgg tcgaggatcg caccagcctg gccggcgaag tccttgctca gcaccgcacc cagcatcagg ccgcgaccac gcacctcgct gaacacgccg aactcttcgt tgatgcgcgc gaagccatcg cgcagcgcct tcgactggcg gctgacgttg gcggcgatct ccgacgaggc cagcttgcgc agcgccacgc gggccaccgc agcggccagc gggttgccac cgaaggtggt gccgtgcgcg ccaaactgca tggtctcggc caccttcggg ccggccagca tcgcaccgat cgggaagccg ccacccagtg ccttggccag ggtgaccatg tccggcacca cgtcgtcctg ccagtgcgcg aacagcgtgc cggtgcggcc catgccggcc tggatctcgt ccagcaccag cagcgcgtcg tgctggtcgc acagttcgcg cacgcgcttg aggaagccgg acttggccgg catcacaccg ccctcgccct ggatcggctc cagcatcacc gccgccacgt caccggcggc catcgcggtt tccagctgca cttcgtcgtt gaaatcgatg tagcggaagc cacccggcag cggctcgtag ccttcctggt acttcggctg cgcggtagcg gtcaccgcgc ccatggtgcg gccgtggaag ctgccacgga aggtgatgat gacgcgcttg tcggccgggc ggccctggct ggaggcccac ttgcggacca tcttgatcgc cacttcgttg gcttcggcgc cggagttgca caggaacacc cgctcggcga agcgactggc cttcaccagt tcctcggcca ggtgcagcgg cggtgcgctg tagaacacgt tgctggtgtg ccagagcttg ccggcctgct cgaccagcgc ggccaccagg tccggatcgt tgtggcccag gccgcacacg gcgatgccgg cagccaggtc gatgaactca cggccctggc tgtcccatac gcgggcgccc tggcctcgct ccagtaccac ctggcggggc ttgtagaccg gcaggtaata gtgcgaaagg gagatcagcg gatcggtcgc ggcggtcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMT AATDPLISLS HYYLPVYKPR QVVLERGQGA RVWDSQGREF IDLAAGIAVC GLGHNDPDLV AALVEQAGKL WHTSNVFYSA PPLHLAEELV KASRFAERVF LCNSGAEANE VAIKMVRKWA SSQGRPADKR VIITFRGSFH GRTMGAVTAT AQPKYQEGYE PLPGGFRYID FNDEVQLETA MAAGDVAAVM LEPIQGEGGV MPAKSGFLKR VRELCDQHDA LLVLDEIQAG MGRTGTLFAH WQDDVVPDMV TLAKALGGGF PIGAMLAGPK VAETMQFGAH GTTFGGNPLA AAVARVALRK LASSEIAANV SRQSKALRDG FARINEEFGV FSEVRGRGLM LGAVLSKDFA GQAGAILDHA AGQGLLTLQA GPDVLRFVPS LNITDEEVAE GLKRLRAAIA AFIAAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCG CCGCGACCGA TCCGCTGATC TCCCTTTCGC ACTATTACCT GCCGGTCTAC AAGCCCCGCC AGGTGGTACT GGAGCGAGGC CAGGGCGCCC GCGTATGGGA CAGCCAGGGC CGTGAGTTCA TCGACCTGGC TGCCGGCATC GCCGTGTGCG GCCTGGGCCA CAACGATCCG GACCTGGTGG CCGCGCTGGT CGAGCAGGCC GGCAAGCTCT GGCACACCAG CAACGTGTTC TACAGCGCAC CGCCGCTGCA CCTGGCCGAG GAACTGGTGA AGGCCAGTCG CTTCGCCGAG CGGGTGTTCC TGTGCAACTC CGGCGCCGAA GCCAACGAAG TGGCGATCAA GATGGTCCGC AAGTGGGCCT CCAGCCAGGG CCGCCCGGCC GACAAGCGCG TCATCATCAC CTTCCGTGGC AGCTTCCACG GCCGCACCAT GGGCGCGGTG ACCGCTACCG CGCAGCCGAA GTACCAGGAA GGCTACGAGC CGCTGCCGGG TGGCTTCCGC TACATCGATT TCAACGACGA AGTGCAGCTG GAAACCGCGA TGGCCGCCGG TGACGTGGCG GCGGTGATGC TGGAGCCGAT CCAGGGCGAG GGCGGTGTGA TGCCGGCCAA GTCCGGCTTC CTCAAGCGCG TGCGCGAACT GTGCGACCAG CACGACGCGC TGCTGGTGCT GGACGAGATC CAGGCCGGCA TGGGCCGCAC CGGCACGCTG TTCGCGCACT GGCAGGACGA CGTGGTGCCG GACATGGTCA CCCTGGCCAA GGCACTGGGT GGCGGCTTCC CGATCGGTGC GATGCTGGCC GGCCCGAAGG TGGCCGAGAC CATGCAGTTT GGCGCGCACG GCACCACCTT CGGTGGCAAC CCGCTGGCCG CTGCGGTGGC CCGCGTGGCG CTGCGCAAGC TGGCCTCGTC GGAGATCGCC GCCAACGTCA GCCGCCAGTC GAAGGCGCTG CGCGATGGCT TCGCGCGCAT CAACGAAGAG TTCGGCGTGT TCAGCGAGGT GCGTGGTCGC GGCCTGATGC TGGGTGCGGT GCTGAGCAAG GACTTCGCCG GCCAGGCTGG TGCGATCCTC GACCACGCTG CCGGGCAGGG GCTGCTGACC CTGCAGGCCG GCCCGGACGT GCTGCGCTTC GTGCCGTCGC TGAACATCAC CGACGAAGAA GTGGCCGAAG GCCTGAAGCG CCTGCGCGCG GCAATTGCCG CGTTCATCGC CGCGCGCTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT