SSGCID: Target Page

StmaA.01026.a

Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18) (aptA)

CENTER ID:	StmaA.01026.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.01026.a.B1.GE43279	Full length(StmaA.01026.a)		1	427

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.01026.a.B1.PW38741	Full length(StmaA.01026.a)		1	427

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.1506

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPFTANRQFK AAPRVLVRAE GMHYEDVDGR QILDGTAGLW CCNAGHARPR IVEAIVEQAR TLDYSPAFQM GSPPAFALAQ RLAALAPTPL NHVFFTSSGS EAVDTAMKIV LAYHRQRGEG QRTRFISREK AYHGVGFGGM ALGGLPNNRR AFGLQLGGVD YLRHTLDLQR NAFSKGLPRQ GAELADDLER LIALHDASTI AAVFVEPIAG SAGVILPAPG YLQRLRELCD HHGILLVFDE VITGFGRVGM PFAAQRFGVT PDLLTFAKAV SNGAVPLGGV LASDAVHATL MQAPPQAIEL FHGYTCSGHP LACAAALATL EVYAEERLFE RAIELGEYWQ ERLHALQGLP NVIDIRNFGL VGAAELAPRR DAPGSRGYEV YRRCFHDGGL LVRCTGDIIA LSPPLIVDKA QIDQITGTLG EMIRATA

NT Sequence

ATGCCGTTCA CCGCCAACCG CCAGTTCAAG GCCGCGCCGC GTGTGCTGGT GCGTGCCGAG GGCATGCACT ACGAGGATGT CGACGGCCGC CAGATCCTCG ATGGCACCGC CGGCCTCTGG TGCTGCAACG CGGGCCATGC CCGCCCACGC ATCGTCGAGG CCATCGTCGA GCAGGCACGC ACGCTCGACT ACTCGCCGGC CTTCCAGATG GGCTCACCGC CGGCCTTCGC GCTGGCGCAG CGACTGGCCG CATTGGCGCC GACGCCACTG AACCATGTGT TCTTCACCAG CTCCGGCTCG GAGGCGGTGG ATACCGCGAT GAAGATCGTG CTGGCCTACC ACCGCCAGCG TGGCGAGGGC CAGCGCACGC GTTTCATCAG CCGCGAGAAG GCCTACCACG GCGTGGGCTT CGGCGGCATG GCGCTGGGCG GCTTGCCCAA CAACCGCAGG GCGTTCGGCC TGCAGCTGGG CGGCGTGGAC TACCTGCGCC ACACCCTGGA CCTGCAGCGC AATGCGTTCA GCAAAGGCCT GCCGCGCCAG GGCGCCGAGC TGGCCGATGA CCTGGAACGA CTGATCGCAC TGCATGATGC CTCAACCATC GCGGCGGTCT TCGTCGAACC CATTGCCGGT TCCGCCGGGG TGATTCTGCC GGCGCCGGGC TACCTGCAGC GCCTGCGCGA GCTGTGCGAC CACCACGGCA TCCTGCTGGT GTTCGATGAG GTCATCACCG GTTTCGGCAG GGTCGGCATG CCATTCGCCG CACAGCGCTT CGGGGTCACC CCCGACCTGC TGACCTTCGC CAAGGCGGTC AGCAACGGCG CAGTGCCACT GGGCGGCGTG CTGGCCAGCG ACGCCGTGCA TGCAACCCTG ATGCAGGCAC CGCCACAGGC CATCGAGCTG TTCCATGGCT ATACCTGTTC GGGGCATCCG CTGGCCTGCG CGGCCGCACT GGCCACGCTG GAGGTCTACG CCGAGGAGCG CCTTTTCGAG CGCGCCATCG AGCTGGGCGA GTACTGGCAG GAACGGCTGC ATGCGCTGCA GGGCCTCCCC AACGTGATCG ACATCCGCAA CTTCGGTCTG GTCGGCGCGG CCGAGCTGGC GCCTCGCCGC GATGCGCCGG GCAGCCGGGG CTACGAGGTC TATCGGCGCT GCTTCCACGA CGGCGGGCTG CTGGTGCGCT GTACCGGTGA CATCATCGCG CTGTCGCCCC CGCTGATCGT GGACAAGGCG CAGATCGACC AGATCACCGG CACGCTGGGC GAGATGATCC GGGCCACTGC C

Details for StmaA.01026.a.B1.GE43279

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	47kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	73
PERCENT COVERAGE:	62

Validated AA Sequence

MAHHHHHHMP XTAXRQFKXA PRVLVRAEGM HYEDVDGRXI LXGXAGLWXC NAGHARXXIX EAIVXXARTL DYSPXFXMGS PPXFXLXXRL XXLAPTPLNH XFFTXSGSEA XDTAMXIVLA YXRQRGEGXX XRVSSAXEKA YHGVGFGGMA LGGLXNNRRA FGLQLGGXXY LRHTLDLQRN AFSKGLPRQG AELADDLERL IALHDASTIA AVFVEPIAGX RRGDSAGAGL PAAPARAVRP PRHPAGVRXG HHRFRQGXAC HSPHSASGSP PTC

Validated NT Sequence

gtgncggtga tctggncgat ctgngccttg tccncnatca gcgggggcga cnnnncgatg atgtcaccgg tacngcgcac cancagcccg ccgtcgngna agcagcgccg atagacctcg tagccccggn tgcccggcgc ntcgcggcga ggcgccagct cggccgcgcc gaccagaccg aagttgcgga tgtcgatnnc nttggggagg ccctgcancg catgcagccg ttcctgccag tactcnccca gctcgatggc gcgctcgaaa aggngctcct cggcgtanac ctccngcgng gccantgcgg ccgcgcaggc cnnnnnatgc cccgaacagg tatagncntg gaacagctcg atggcctgtg gcggngcctg cntcanggtt gcatgcacgg cgtcgctggc cancacgccg cccagtggca ctgcgccgtt gctgaccgcc ttggcgaagg tcagcaggtc gggggtgacc ccgaagcgct gtgcggcgaa tggcatgccn gaccctgccg aaaccggtga tgacctcntc gaacaccagc aggatgccgt ggtggtcgca cagctcgcgc aggcgctgca ggtagcccgg cgccggcaga atcaccccgg cggnaaccgg caatgggttc gacgaagacc gccgcgatgg ttgaggcatc atgcagtgcg atcagtcgtt ccaggtcatc ggccagctcg gcgccctggc gcggcaggcc tttgctgaac gcattgcgct gcaggtccag ggtgtggcgc aggtagnccn nnccgcccag ctgcaggccg aacgccctgc ggttgttggn caagccgccc agcgccatgc cgccgaagcc cacgccgtgg taggccttct cncnggctga tgaaacgcgt nnnncnnggc cctcgccacg ctggcggngg taggccagca cgatnttcat ngcggtatcc nccgcctccg agccggagnt ggtgaagaac ncatggttca gtggngtcgg cgccaatnng nncagtcgnt gcnccagcgn gaagnccggc ggtgagccca tntggaagnc cggngagtag tcgagcgtgc gtgcntnntn gacgatggcc tcgncgatgn nnngncgggc atggcccgcg ttgcagcncc agaggccggc ggnnccatng aggatntggc ggccgtcgac atcctcgtag tgcatgccct cggcncgcac cagcacacgc ggcgcgncct tgaactggcg gtnggcggtg ancggcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMP FTANRQFKAA PRVLVRAEGM HYEDVDGRQI LDGTAGLWCC NAGHARPRIV EAIVEQARTL DYSPAFQMGS PPAFALAQRL AALAPTPLNH VFFTSSGSEA VDTAMKIVLA YHRQRGEGQR TRFISREKAY HGVGFGGMAL GGLPNNRRAF GLQLGGVDYL RHTLDLQRNA FSKGLPRQGA ELADDLERLI ALHDASTIAA VFVEPIAGSA GVILPAPGYL QRLRELCDHH GILLVFDEVI TGFGRVGMPF AAQRFGVTPD LLTFAKAVSN GAVPLGGVLA SDAVHATLMQ APPQAIELFH GYTCSGHPLA CAAALATLEV YAEERLFERA IELGEYWQER LHALQGLPNV IDIRNFGLVG AAELAPRRDA PGSRGYEVYR RCFHDGGLLV RCTGDIIALS PPLIVDKAQI DQITGTLGEM IRATA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgt tcaccgccaa ccgccagttc aaggccgcgc cgcgtgtgct ggtgcgtgcc gagggcatgc actacgagga tgtcgacggc cgccagatcc tcgatggcac cgccggcctc tggtgctgca acgcgggcca tgcccgccca cgcatcgtcg aggccatcgt cgagcaggca cgcacgctcg actactcgcc ggccttccag atgggctcac cgccggcctt cgcgctggcg cagcgactgg ccgcattggc gccgacgcca ctgaaccatg tgttcttcac cagctccggc tcggaggcgg tggataccgc gatgaagatc gtgctggcct accaccgcca gcgtggcgag ggccagcgca cgcgtttcat cagccgcgag aaggcctacc acggcgtggg cttcggcggc atggcgctgg gcggcttgcc caacaaccgc agggcgttcg gcctgcagct gggcggcgtg gactacctgc gccacaccct ggacctgcag cgcaatgcgt tcagcaaagg cctgccgcgc cagggcgccg agctggccga tgacctggaa cgactgatcg cactgcatga tgcctcaacc atcgcggcgg tcttcgtcga acccattgcc ggttccgccg gggtgattct gccggcgccg ggctacctgc agcgcctgcg cgagctgtgc gaccaccacg gcatcctgct ggtgttcgat gaggtcatca ccggtttcgg cagggtcggc atgccattcg ccgcacagcg cttcggggtc acccccgacc tgctgacctt cgccaaggcg gtcagcaacg gcgcagtgcc actgggcggc gtgctggcca gcgacgccgt gcatgcaacc ctgatgcagg caccgccaca ggccatcgag ctgttccatg gctatacctg ttcggggcat ccgctggcct gcgcggccgc actggccacg ctggaggtct acgccgagga gcgccttttc gagcgcgcca tcgagctggg cgagtactgg caggaacggc tgcatgcgct gcagggcctc cccaacgtga tcgacatccg caacttcggt ctggtcggcg cggccgagct ggcgcctcgc cgcgatgcgc cgggcagccg gggctacgag gtctatcggc gctgcttcca cgacggcggg ctgctggtgc gctgtaccgg tgacatcatc gcgctgtcgc ccccgctgat cgtggacaag gcgcagatcg accagatcac cggcacgctg ggcgagatga tccgggccac tgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for StmaA.01026.a.B1.PW38741

PURIFICATION DATe:	10/24/2019
CONCENTRATION:	49.3mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	73
PERCENT COVERAGE:	62

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGT TCACCGCCAA CCGCCAGTTC AAGGCCGCGC CGCGTGTGCT GGTGCGTGCC GAGGGCATGC ACTACGAGGA TGTCGACGGC CGCCAGATCC TCGATGGCAC CGCCGGCCTC TGGTGCTGCA ACGCGGGCCA TGCCCGCCCA CGCATCGTCG AGGCCATCGT CGAGCAGGCA CGCACGCTCG ACTACTCGCC GGCCTTCCAG ATGGGCTCAC CGCCGGCCTT CGCGCTGGCG CAGCGACTGG CCGCATTGGC GCCGACGCCA CTGAACCATG TGTTCTTCAC CAGCTCCGGC TCGGAGGCGG TGGATACCGC GATGAAGATC GTGCTGGCCT ACCACCGCCA GCGTGGCGAG GGCCAGCGCA CGCGTTTCAT CAGCCGCGAG AAGGCCTACC ACGGCGTGGG CTTCGGCGGC ATGGCGCTGG GCGGCTTGCC CAACAACCGC AGGGCGTTCG GCCTGCAGCT GGGCGGCGTG GACTACCTGC GCCACACCCT GGACCTGCAG CGCAATGCGT TCAGCAAAGG CCTGCCGCGC CAGGGCGCCG AGCTGGCCGA TGACCTGGAA CGACTGATCG CACTGCATGA TGCCTCAACC ATCGCGGCGG TCTTCGTCGA ACCCATTGCC GGTTCCGCCG GGGTGATTCT GCCGGCGCCG GGCTACCTGC AGCGCCTGCG CGAGCTGTGC GACCACCACG GCATCCTGCT GGTGTTCGAT GAGGTCATCA CCGGTTTCGG CAGGGTCGGC ATGCCATTCG CCGCACAGCG CTTCGGGGTC ACCCCCGACC TGCTGACCTT CGCCAAGGCG GTCAGCAACG GCGCAGTGCC ACTGGGCGGC GTGCTGGCCA GCGACGCCGT GCATGCAACC CTGATGCAGG CACCGCCACA GGCCATCGAG CTGTTCCATG GCTATACCTG TTCGGGGCAT CCGCTGGCCT GCGCGGCCGC ACTGGCCACG CTGGAGGTCT ACGCCGAGGA GCGCCTTTTC GAGCGCGCCA TCGAGCTGGG CGAGTACTGG CAGGAACGGC TGCATGCGCT GCAGGGCCTC CCCAACGTGA TCGACATCCG CAACTTCGGT CTGGTCGGCG CGGCCGAGCT GGCGCCTCGC CGCGATGCGC CGGGCAGCCG GGGCTACGAG GTCTATCGGC GCTGCTTCCA CGACGGCGGG CTGCTGGTGC GCTGTACCGG TGACATCATC GCGCTGTCGC CCCCGCTGAT CGTGGACAAG GCGCAGATCG ACCAGATCAC CGGCACGCTG GGCGAGATGA TCCGGGCCAC TGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT