StmaA.01019.a
Dihydropteroate synthase (EC 2.5.1.15) (folP)
| CENTER ID: | StmaA.01019.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01019.a.B1.GE43277 | Full length(StmaA.01019.a) | 1 | 298 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.01019.a.B1.PW38739 | Full length(StmaA.01019.a) | 1 | 298 |
Structures
7L6P
DEPOSITED: 12/23/2020
DETERMINATION: XRay
CLONE: StmaA.01019.a.B1.GE43277
PROTEIN: StmaA.01019.a.B1.PW38739
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.1657 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFDTSPQLDC AGRILRLDRA RVMGIVNVTP DSFSDGGAHD TTEAAVAHGL KLVEEGADLL DIGGESTRPG AAPVSVEEEL RRVIPVIEQL AARTRVPISI DTFKPEVMRA AVAAGAGMIN DIYGLRQEGA LDAAAATGVP VVLMHMQGEP GHMQADPHYD DVVAEVHGFL VQRLFAAEMA GFAKKNLLID LGFGFGKTTA HNMTLLARSE RFLELGVPML AGLSRKRSLG ELTGRDTPSE RVAASVAAHL IAVQRGARIV RVHDVAATVD ALKIWQAVEA VPTPRADATP TIRWPDED
NT Sequence
ATGTTCGATA CCTCGCCCCA GCTCGATTGC GCCGGCCGCA TCCTGCGCCT CGACCGTGCC CGGGTCATGG GCATCGTCAA CGTCACCCCG GACTCCTTCT CCGATGGCGG CGCGCACGAC ACCACCGAGG CTGCGGTCGC CCATGGCCTG AAGCTGGTCG AGGAGGGCGC GGACCTGCTG GACATCGGCG GCGAATCGAC CCGGCCGGGC GCTGCGCCGG TATCGGTGGA GGAGGAACTG CGCCGGGTGA TTCCGGTGAT CGAGCAGCTG GCCGCACGCA CCCGGGTGCC GATCAGCATC GACACCTTCA AGCCGGAAGT GATGCGCGCG GCCGTGGCGG CTGGCGCTGG CATGATCAAT GACATCTACG GACTGCGTCA GGAAGGGGCG CTGGACGCCG CCGCTGCAAC CGGCGTGCCG GTGGTGCTGA TGCACATGCA GGGCGAGCCA GGCCACATGC AGGCCGATCC ACATTACGAC GACGTGGTCG CCGAGGTGCA CGGCTTCCTG GTCCAGCGCC TGTTCGCTGC CGAGATGGCG GGCTTTGCGA AGAAGAACCT GTTGATCGAT CTCGGTTTCG GTTTCGGCAA AACCACCGCC CACAACATGA CCCTGCTGGC ACGCTCCGAG CGGTTCCTGG AGCTGGGCGT GCCGATGCTG GCTGGCCTGT CGCGCAAGCG CAGCCTGGGC GAACTGACCG GTCGCGACAC GCCGTCCGAG CGCGTGGCCG CCTCGGTGGC CGCGCACCTG ATCGCGGTGC AGCGTGGCGC ACGCATCGTG CGCGTGCATG ACGTGGCCGC CACCGTTGAT GCATTGAAGA TCTGGCAGGC AGTCGAGGCC GTGCCAACGC CGCGCGCCGA CGCCACGCCG ACGATCCGCT GGCCGGACGA AGAC
Details for StmaA.01019.a.B1.GE43277
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMF DTSPQLDCAG RILRLDRARV MGIVNVTPDS FSDGGAHDTT EAAVAHGLKL VEEGADLLDI GGESTRPGAA PVSVEEELRR VIPVIEQLAA RTRVPISIDT FKPEVMRAAV AAGAGMINDI YGLRQEGALD AAAATGVPVV LMHMQGEPGH MQADPHYDDV VAEVHGFLVQ RLFAAEMAGF AKKNLLIDLG FGFGKTTAHN MTLLARSERF LELGVPMLAG LSRKRSLGEL TGRDTPSERV AASVAAHLIA VQRGARIVRV HDVAATVDAL KIWQAVEAVP TPRADATP
Validated NT Sequence
tcggcgtggc gtcggcgcgc ggcgttggca cggcctcgac tgcctgccag atcttcaatg catcaacggt ggcggccacg tcatgcacgc gcacgatgcg tgcgccacgc tgcaccgcga tcaggtgcgc ggccaccgag gcggccacgc gctcggacgg cgtgtcgcga ccggtcagtt cgcccaggct gcgcttgcgc gacaggccag ccagcatcgg cacgcccagc tccaggaacc gctcggagcg tgccagcagg gtcatgttgt gggcggtggt tttgccgaaa ccgaaaccga gatcgatcaa caggttcttc ttcgcaaagc ccgccatctc ggcagcgaac aggcgctgga ccaggaagcc gtgcacctcg gcgaccacgt cgtcgtaatg tggatcggcc tgcatgtggc ctggctcgcc ctgcatgtgc atcagcacca ccggcacgcc ggttgcagcg gcggcgtcca gcgccccttc ctgacgcagt ccgtagatgt cattgatcat gccagcgcca gccgccacgg ccgcgcgcat cacttccggc ttgaaggtgt cgatgctgat cggcacccgg gtgcgtgcgg ccagctgctc gatcaccgga atcacccggc gcagttcctc ctccaccgat accggcgcag cgcccggccg ggtcgattcg ccgccgatgt ccagcaggtc cgcgccctcc tcgaccagct tcaggccatg ggcgaccgca gcctcggtgg tgtcgtgcgc gccgccatcg gagaaggagt ccggggtgac gttgacgatg cccatgaccc gggcacggtc gaggcgcagg atgcggccgg cgcaatcgag ctggggcgag gtatcgaaca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMF DTSPQLDCAG RILRLDRARV MGIVNVTPDS FSDGGAHDTT EAAVAHGLKL VEEGADLLDI GGESTRPGAA PVSVEEELRR VIPVIEQLAA RTRVPISIDT FKPEVMRAAV AAGAGMINDI YGLRQEGALD AAAATGVPVV LMHMQGEPGH MQADPHYDDV VAEVHGFLVQ RLFAAEMAGF AKKNLLIDLG FGFGKTTAHN MTLLARSERF LELGVPMLAG LSRKRSLGEL TGRDTPSERV AASVAAHLIA VQRGARIVRV HDVAATVDAL KIWQAVEAVP TPRADATPTI RWPDED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg atacctcgcc ccagctcgat tgcgccggcc gcatcctgcg cctcgaccgt gcccgggtca tgggcatcgt caacgtcacc ccggactcct tctccgatgg cggcgcgcac gacaccaccg aggctgcggt cgcccatggc ctgaagctgg tcgaggaggg cgcggacctg ctggacatcg gcggcgaatc gacccggccg ggcgctgcgc cggtatcggt ggaggaggaa ctgcgccggg tgattccggt gatcgagcag ctggccgcac gcacccgggt gccgatcagc atcgacacct tcaagccgga agtgatgcgc gcggccgtgg cggctggcgc tggcatgatc aatgacatct acggactgcg tcaggaaggg gcgctggacg ccgccgctgc aaccggcgtg ccggtggtgc tgatgcacat gcagggcgag ccaggccaca tgcaggccga tccacattac gacgacgtgg tcgccgaggt gcacggcttc ctggtccagc gcctgttcgc tgccgagatg gcgggctttg cgaagaagaa cctgttgatc gatctcggtt tcggtttcgg caaaaccacc gcccacaaca tgaccctgct ggcacgctcc gagcggttcc tggagctggg cgtgccgatg ctggctggcc tgtcgcgcaa gcgcagcctg ggcgaactga ccggtcgcga cacgccgtcc gagcgcgtgg ccgcctcggt ggccgcgcac ctgatcgcgg tgcagcgtgg cgcacgcatc gtgcgcgtgc atgacgtggc cgccaccgtt gatgcattga agatctggca ggcagtcgag gccgtgccaa cgccgcgcgc cgacgccacg ccgacgatcc gctggccgga cgaagactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for StmaA.01019.a.B1.PW38739
| PURIFICATION DATe: | 10/23/2019 |
| CONCENTRATION: | 16.2mg/ml |
| OBSERVED MW: | 33kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 13 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF DTSPQLDCAG RILRLDRARV MGIVNVTPDS FSDGGAHDTT EAAVAHGLKL VEEGADLLDI GGESTRPGAA PVSVEEELRR VIPVIEQLAA RTRVPISIDT FKPEVMRAAV AAGAGMINDI YGLRQEGALD AAAATGVPVV LMHMQGEPGH MQADPHYDDV VAEVHGFLVQ RLFAAEMAGF AKKNLLIDLG FGFGKTTAHN MTLLARSERF LELGVPMLAG LSRKRSLGEL TGRDTPSERV AASVAAHLIA VQRGARIVRV HDVAATVDAL KIWQAVEAVP TPRADATP
Validated NT Sequence
tcggcgtggc gtcggcgcgc ggcgttggca cggcctcgac tgcctgccag atcttcaatg catcaacggt ggcggccacg tcatgcacgc gcacgatgcg tgcgccacgc tgcaccgcga tcaggtgcgc ggccaccgag gcggccacgc gctcggacgg cgtgtcgcga ccggtcagtt cgcccaggct gcgcttgcgc gacaggccag ccagcatcgg cacgcccagc tccaggaacc gctcggagcg tgccagcagg gtcatgttgt gggcggtggt tttgccgaaa ccgaaaccga gatcgatcaa caggttcttc ttcgcaaagc ccgccatctc ggcagcgaac aggcgctgga ccaggaagcc gtgcacctcg gcgaccacgt cgtcgtaatg tggatcggcc tgcatgtggc ctggctcgcc ctgcatgtgc atcagcacca ccggcacgcc ggttgcagcg gcggcgtcca gcgccccttc ctgacgcagt ccgtagatgt cattgatcat gccagcgcca gccgccacgg ccgcgcgcat cacttccggc ttgaaggtgt cgatgctgat cggcacccgg gtgcgtgcgg ccagctgctc gatcaccgga atcacccggc gcagttcctc ctccaccgat accggcgcag cgcccggccg ggtcgattcg ccgccgatgt ccagcaggtc cgcgccctcc tcgaccagct tcaggccatg ggcgaccgca gcctcggtgg tgtcgtgcgc gccgccatcg gagaaggagt ccggggtgac gttgacgatg cccatgaccc gggcacggtc gaggcgcagg atgcggccgg cgcaatcgag ctggggcgag gtatcgaaca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMF DTSPQLDCAG RILRLDRARV MGIVNVTPDS FSDGGAHDTT EAAVAHGLKL VEEGADLLDI GGESTRPGAA PVSVEEELRR VIPVIEQLAA RTRVPISIDT FKPEVMRAAV AAGAGMINDI YGLRQEGALD AAAATGVPVV LMHMQGEPGH MQADPHYDDV VAEVHGFLVQ RLFAAEMAGF AKKNLLIDLG FGFGKTTAHN MTLLARSERF LELGVPMLAG LSRKRSLGEL TGRDTPSERV AASVAAHLIA VQRGARIVRV HDVAATVDAL KIWQAVEAVP TPRADATPTI RWPDED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCG ATACCTCGCC CCAGCTCGAT TGCGCCGGCC GCATCCTGCG CCTCGACCGT GCCCGGGTCA TGGGCATCGT CAACGTCACC CCGGACTCCT TCTCCGATGG CGGCGCGCAC GACACCACCG AGGCTGCGGT CGCCCATGGC CTGAAGCTGG TCGAGGAGGG CGCGGACCTG CTGGACATCG GCGGCGAATC GACCCGGCCG GGCGCTGCGC CGGTATCGGT GGAGGAGGAA CTGCGCCGGG TGATTCCGGT GATCGAGCAG CTGGCCGCAC GCACCCGGGT GCCGATCAGC ATCGACACCT TCAAGCCGGA AGTGATGCGC GCGGCCGTGG CGGCTGGCGC TGGCATGATC AATGACATCT ACGGACTGCG TCAGGAAGGG GCGCTGGACG CCGCCGCTGC AACCGGCGTG CCGGTGGTGC TGATGCACAT GCAGGGCGAG CCAGGCCACA TGCAGGCCGA TCCACATTAC GACGACGTGG TCGCCGAGGT GCACGGCTTC CTGGTCCAGC GCCTGTTCGC TGCCGAGATG GCGGGCTTTG CGAAGAAGAA CCTGTTGATC GATCTCGGTT TCGGTTTCGG CAAAACCACC GCCCACAACA TGACCCTGCT GGCACGCTCC GAGCGGTTCC TGGAGCTGGG CGTGCCGATG CTGGCTGGCC TGTCGCGCAA GCGCAGCCTG GGCGAACTGA CCGGTCGCGA CACGCCGTCC GAGCGCGTGG CCGCCTCGGT GGCCGCGCAC CTGATCGCGG TGCAGCGTGG CGCACGCATC GTGCGCGTGC ATGACGTGGC CGCCACCGTT GATGCATTGA AGATCTGGCA GGCAGTCGAG GCCGTGCCAA CGCCGCGCGC CGACGCCACG CCGACGATCC GCTGGCCGGA CGAAGACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT