StmaA.00983.a

Flagellar biosynthesis protein FlhF (flhF)

CENTER ID: StmaA.00983.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00983.a.B1.GE43275 Full length(StmaA.00983.a) 1 540
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.2166

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKIKRFVAAD MRSAMNLVRK EHGPDAVILS NRRIEEGVEI VAAANYDESA VQRALEASRR DVAPPPAPKP RTAADAVIAA VTRRRSAAPA PEPVAATTSA VAALARAAVG ATGRTLDSAD EIVPTRGSTG FAATLARAAV NEPALPEQIF APFAEAIVAP GPAVTAPANR ARFQLDPPHD THHESVPPAV QPPPLPGAAM ADVQPDIAAS ETAALEAAGE ASPAPMLAPA PTLTVVAQDD AEIRQLRQEV AGMRQVIERE MNRFTDERLR GSPVRATALD LMDEYGFDAG LARDVAMQIP LETEAHRGRG LMLGLISRKL PIAPVDPLEE GGVIALVGPT GAGKTTTIAK LASRFAEKHA PRDVALVTTD TTRIGAREQL YGYGRQLGIA VHEANSGTDL DQLLERLKDY KLVLIDTAGL GPRDRALAAQ LQWLRAARQV RTLLVLPANT SFGDMDEVVR RFGAANLQGL VLSKLDETGR FGNALSVAVD HALPINWVTD GQDVPEDLHR ASAANLVLRL EDLRRAADMP CNPELNHAVA
NT Sequence
ATGAAAATCA AACGATTCGT CGCCGCCGAC ATGCGCTCGG CCATGAACCT GGTGCGCAAG GAACATGGTC CTGACGCCGT GATCCTGTCC AACCGCCGGA TCGAGGAAGG CGTCGAGATC GTCGCCGCGG CCAACTACGA CGAGAGCGCC GTGCAGCGTG CACTGGAAGC CTCGCGCCGT GATGTCGCAC CGCCGCCGGC TCCCAAGCCG CGCACCGCTG CTGACGCGGT GATCGCCGCG GTCACCCGCC GCCGCAGCGC TGCCCCGGCG CCGGAGCCGG TGGCCGCCAC CACCTCGGCC GTGGCCGCCC TCGCCCGTGC GGCCGTGGGT GCCACCGGCC GCACCTTGGA CAGCGCCGAC GAGATCGTGC CGACCCGCGG CAGCACCGGC TTCGCAGCCA CCCTGGCGCG CGCCGCCGTC AACGAACCGG CGCTGCCCGA ACAGATCTTC GCCCCGTTCG CCGAGGCCAT CGTCGCACCG GGCCCGGCCG TCACCGCGCC GGCCAACCGC GCCCGCTTCC AGCTCGACCC GCCGCACGAC ACGCATCACG AAAGCGTGCC CCCCGCCGTG CAGCCGCCGC CGCTGCCGGG CGCTGCCATG GCCGATGTCC AGCCTGACAT CGCCGCCAGC GAAACGGCAG CGCTCGAAGC CGCCGGCGAA GCCAGCCCGG CCCCCATGCT GGCCCCGGCA CCGACGCTGA CCGTGGTTGC CCAGGACGAC GCCGAGATCC GCCAGCTGCG CCAGGAAGTG GCCGGCATGC GCCAGGTGAT CGAGCGCGAG ATGAACCGCT TCACCGACGA GCGCCTGCGT GGCAGCCCGG TGCGCGCCAC CGCGCTGGAC CTGATGGACG AGTACGGTTT CGACGCCGGG CTGGCCCGCG ACGTGGCCAT GCAGATCCCG CTGGAGACCG AAGCCCATCG TGGCCGCGGC CTGATGCTGG GGCTGATCTC GCGCAAGCTG CCGATCGCCC CGGTCGACCC GCTGGAAGAA GGCGGCGTGA TCGCCCTGGT CGGCCCGACC GGCGCCGGCA AGACCACCAC CATCGCCAAG CTGGCCTCGC GCTTCGCCGA GAAGCATGCG CCGCGCGATG TGGCCCTGGT GACCACCGAC ACCACCCGCA TCGGCGCCCG CGAGCAGCTG TACGGCTACG GCCGCCAGCT CGGCATCGCG GTGCACGAGG CCAACAGCGG CACCGACCTG GACCAGCTGC TGGAACGCCT GAAGGACTAC AAGCTGGTGC TGATCGACAC CGCCGGCCTG GGCCCGCGCG ACCGCGCGCT GGCCGCGCAG CTGCAGTGGC TGCGCGCCGC CCGCCAGGTA CGCACCCTGC TGGTGCTGCC GGCCAACACC AGCTTCGGCG ACATGGACGA GGTGGTCCGC CGCTTCGGCG CCGCCAACCT GCAGGGCCTG GTGCTGAGCA AGCTGGACGA GACCGGCCGC TTCGGCAACG CCCTGTCAGT GGCCGTGGAC CACGCCCTCC CGATCAACTG GGTGACCGAT GGCCAGGACG TTCCGGAGGA CCTGCACCGG GCCAGTGCGG CCAATCTCGT ACTTCGCCTT GAAGATTTGC GCCGCGCGGC CGATATGCCC TGCAACCCGG AGTTGAACCA TGCCGTCGCG
Details for StmaA.00983.a.B1.GE43275
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 58kDa
OBSERVED MW: 29kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK IKRFVAADMR SAMNLVRKEH GPDAVILSNR RIEEGVEIVA AANYDESAVQ RALEASRRDV APPPAPKPRT AADAVIAAVT RRRSAAPAPE PVAATTSAVA ALARAAVGAT GRTLDSADEI VPTRGSTGFA ATLARAAVNE PALPEQIFAP FAEAIVAPGP AVTAPANRAR FQLDPPHDTH HESVPPAVQP PPLPGAAMAD VQPDIAASET AALEAAGEAS PAPMLAPAPT LTVVAQDDAE IRQLRQEVAG MRQVIEREMN RFTDERLRGS PVRATALDLM DEYGFDAGLA RDVAMQIPLE TEAHRGRGLM LGLISRKLPI APVDPLEEGG VIALVGPTGA GKTTTIAKLA SRFAEKHAPR DVALVTTDTT RIGAREQLYG YGRQLGIAVH EANSGTDLDQ LLERLKDYKL VLIDTAGLGP RDRALAAQLQ WLRAARQVRT LLVLPANTSF GDMDEVVRRF GAANLQGLVL SKLDETGRFG NALSVAVDHA LPINWVTDGQ DVPEDLHRAS AANLVLRLED LRRAADMPCN PELNHAVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa tcaaacgatt cgtcgccgcc gacatgcgct cggccatgaa cctggtgcgc aaggaacatg gtcctgacgc cgtgatcctg tccaaccgcc ggatcgagga aggcgtcgag atcgtcgccg cggccaacta cgacgagagc gccgtgcagc gtgcactgga agcctcgcgc cgtgatgtcg caccgccgcc ggctcccaag ccgcgcaccg ctgctgacgc ggtgatcgcc gcggtcaccc gccgccgcag cgctgccccg gcgccggagc cggtggccgc caccacctcg gccgtggccg ccctcgcccg tgcggccgtg ggtgccaccg gccgcacctt ggacagcgcc gacgagatcg tgccgacccg cggcagcacc ggcttcgcag ccaccctggc gcgcgccgcc gtcaacgaac cggcgctgcc cgaacagatc ttcgccccgt tcgccgaggc catcgtcgca ccgggcccgg ccgtcaccgc gccggccaac cgcgcccgct tccagctcga cccgccgcac gacacgcatc acgaaagcgt gccccccgcc gtgcagccgc cgccgctgcc gggcgctgcc atggccgatg tccagcctga catcgccgcc agcgaaacgg cagcgctcga agccgccggc gaagccagcc cggcccccat gctggccccg gcaccgacgc tgaccgtggt tgcccaggac gacgccgaga tccgccagct gcgccaggaa gtggccggca tgcgccaggt gatcgagcgc gagatgaacc gcttcaccga cgagcgcctg cgtggcagcc cggtgcgcgc caccgcgctg gacctgatgg acgagtacgg tttcgacgcc gggctggccc gcgacgtggc catgcagatc ccgctggaga ccgaagccca tcgtggccgc ggcctgatgc tggggctgat ctcgcgcaag ctgccgatcg ccccggtcga cccgctggaa gaaggcggcg tgatcgccct ggtcggcccg accggcgccg gcaagaccac caccatcgcc aagctggcct cgcgcttcgc cgagaagcat gcgccgcgcg atgtggccct ggtgaccacc gacaccaccc gcatcggcgc ccgcgagcag ctgtacggct acggccgcca gctcggcatc gcggtgcacg aggccaacag cggcaccgac ctggaccagc tgctggaacg cctgaaggac tacaagctgg tgctgatcga caccgccggc ctgggcccgc gcgaccgcgc gctggccgcg cagctgcagt ggctgcgcgc cgcccgccag gtacgcaccc tgctggtgct gccggccaac accagcttcg gcgacatgga cgaggtggtc cgccgcttcg gcgccgccaa cctgcagggc ctggtgctga gcaagctgga cgagaccggc cgcttcggca acgccctgtc agtggccgtg gaccacgccc tcccgatcaa ctgggtgacc gatggccagg acgttccgga ggacctgcac cgggccagtg cggccaatct cgtacttcgc cttgaagatt tgcgccgcgc ggccgatatg ccctgcaacc cggagttgaa ccatgccgtc gcgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat