StmaA.00980.a
Phosphoserine aminotransferase (EC 2.6.1.52) (serC)
| CENTER ID: | StmaA.00980.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00980.a.B1.GE43274 | Full length(StmaA.00980.a) | 1 | 361 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00980.a.B1.PW38764 | Full length(StmaA.00980.a) | 1 | 361 |
Structures
6XDK
DEPOSITED: 6/10/2020
DETERMINATION: XRay
CLONE: StmaA.00980.a.B1.GE43274
PROTEIN: StmaA.00980.a.B1.PW38764
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.2901 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRAFNFSAG PATLPESVLR QAQAEMLDWH GSGASIVEMS HRGAEFMSVA AEAEADLRRL LDIPDDYAVL FLSGGATTQQ ALIPLNFAAP GQRADYVVSG HWGKTAVKQA GVYVDVNIAA SSEANGYREL PARADWQLSR DAAYVHITAN ETIHGVEFRD VPDTGNVPLI ADFSSSIASE PLDVRRYGVI YAGAQKNLGP VGVAVMIIRR DLLERSGQPR ADIFDYRSHV ARDSMLNTPP TWNWYLAGLV FKWMLAEGGV TEFAKRNAAK AALVYGAIDG SGGFYRNEVA YAARSRMNIP FFLPDAELDA RFVAEAKAAG LLALKGHKVV GGIRASLYNA MPLAGAEALV AFMADFQQRH G
NT Sequence
ATGACGCGCG CGTTCAACTT CAGTGCCGGC CCTGCGACCT TGCCGGAATC GGTCCTGCGC CAGGCGCAGG CGGAAATGCT GGACTGGCAC GGGTCCGGCG CTTCGATCGT GGAAATGAGC CATCGCGGCG CGGAATTCAT GTCCGTGGCT GCCGAAGCCG AGGCCGACCT GCGTCGCCTG CTCGACATCC CCGACGACTA TGCGGTGCTG TTCCTGTCCG GTGGCGCCAC CACCCAGCAG GCACTGATCC CGCTCAACTT CGCCGCCCCG GGCCAGCGTG CCGATTACGT GGTTAGTGGC CACTGGGGCA AGACGGCGGT CAAGCAGGCC GGGGTCTACG TCGACGTCAA CATTGCCGCC AGCAGCGAGG CCAACGGCTA CCGCGAGCTG CCGGCGCGTG CCGACTGGCA GCTGTCGCGC GATGCCGCCT ACGTGCACAT CACCGCCAAC GAGACCATCC ACGGCGTCGA ATTCCGTGAT GTGCCCGACA CCGGCAACGT CCCGCTGATC GCCGACTTCA GCTCGTCCAT CGCCAGCGAA CCGCTGGATG TGCGCCGCTA TGGCGTGATC TACGCCGGCG CGCAGAAGAA CCTGGGCCCG GTCGGCGTCG CGGTGATGAT CATCCGCCGC GACCTGCTCG AGCGCAGTGG CCAGCCGCGC GCGGACATCT TCGATTACCG CTCGCACGTC GCCCGCGATT CGATGCTCAA CACGCCGCCG ACCTGGAACT GGTATCTGGC CGGCCTGGTG TTCAAGTGGA TGCTGGCCGA AGGCGGCGTG ACCGAATTCG CCAAGCGCAA CGCCGCCAAG GCCGCGCTGG TGTACGGCGC CATCGACGGC TCCGGCGGCT TCTACCGCAA TGAAGTCGCA TATGCGGCCC GTTCGCGCAT GAACATTCCG TTCTTCCTGC CCGATGCCGA GCTTGACGCC CGCTTCGTCG CCGAAGCCAA GGCCGCTGGC CTGCTGGCGC TGAAGGGCCA CAAGGTGGTC GGCGGCATCC GGGCTTCGCT GTACAACGCC ATGCCGCTGG CCGGGGCCGA GGCGCTGGTC GCCTTCATGG CCGATTTCCA GCAGCGTCAC GGT
Details for StmaA.00980.a.B1.GE43274
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 40kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 44 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMT XXXXXXXXPX TXXXXVXRXX XXXMXXXXXS XASIXXMXXL XEXFXXVAAX AXXXLRXXXX IPXXYXXLXL SGGAXTXXXX XXXXXXXXXX XXXXXXXXHW XKTAVKQXXV SXDVNIAAXX EAXXXXEXXX RXXXXXSHDX XXVHIXANEX IXXVEFRDXX DTXXVPXIXX XXSSIXSXPL XVRRYXXIYA GXQKNXXXVX VXXXIXXRXL XEXSXXPRXD IXXXXXXVXX XXMXXXPXXW XWYLXXXVFK XXLXXXGVXE XXXXNAXXAX XVYGAIXXXX XXXXNEXAXX XRXRMXXXXF XXXXELXXRX XXXAKAXXXX XLKAHKXVXX IXXSLXNXXX XXXAEALVAX XXXXQQRXX
Validated NT Sequence
atggctcacc accaccacca ccatatgacs mkcrysytcm acwtcrkygy cgsccckknn acctygcmgg amtmsgtcst gcgccangng canrnnnaaa tgcwskaytg snacsggtcc gsngcktcna ttnagnraat gannywycts gnngagkaat tcmtgtmsgt ggctgccsaa gccnaggycr acctgcgwyg scwgmtcsac atccccsann actatgmsgn gctgktcctg tccggyggsg ccrccacynn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nrgccactgg gncaagacgg cggtcaagca ggnngnggtc tccntcgacg tcaacattgc cgccagsagn gaggccancg rstanngcga gntgcnggng cgtrccnact rgmagywgtc gcacgatrny rcttangtgc acatmnnngc saacgagayc atccncgkcg tngaattccg tgatnnrnsn gacaccrgca rcgtnccgnt gatcrccgan ntcagntcgt ccatcnccag yganccgctg gakgtrcgyc gmtatsrcgw ratytaygcc ggcrsgcaga agaacntsgs cmcggtykgc gtcgmggwga wgatcwtcck ccgcgwcctg mtcgarmrca gygkscakcc gcgcsmggac atctwyrakt mcckcwcgca mgtcncccan sakwcsatgy tcracwcgcc smygmcstgg aantggtaty tgsmsgkcnt ggtgttcaag yggatsytgk csgamrrcgg cgtgrcsgaa ktcrmcaasc nsaacgccgm cawggccgmg ntggtgtacg gcgccatcgw srkctmcgkc gknttnyacy gcaaygaast cgcayaygmg ssccgytssc gcatgrwcmw tsmsktcttc nygycsganr ncgagctwgw cryccgctts rtcgsckamg ccaaggccsy tgrcytsmkg smgytgaagg cccacaaggw ggtcgrmgrc atcsrggsyt cgctgyacaa cgmcakgscs mwggmsgrgg csgaggcsct ggtcgcctts ryggncnnyw nccagcagcg tcassrttaa
Expected Protein Sequence
MAHHHHHHMT RAFNFSAGPA TLPESVLRQA QAEMLDWHGS GASIVEMSHR GAEFMSVAAE AEADLRRLLD IPDDYAVLFL SGGATTQQAL IPLNFAAPGQ RADYVVSGHW GKTAVKQAGV YVDVNIAASS EANGYRELPA RADWQLSRDA AYVHITANET IHGVEFRDVP DTGNVPLIAD FSSSIASEPL DVRRYGVIYA GAQKNLGPVG VAVMIIRRDL LERSGQPRAD IFDYRSHVAR DSMLNTPPTW NWYLAGLVFK WMLAEGGVTE FAKRNAAKAA LVYGAIDGSG GFYRNEVAYA ARSRMNIPFF LPDAELDARF VAEAKAAGLL ALKGHKVVGG IRASLYNAMP LAGAEALVAF MADFQQRHG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacgc gcgcgttcaa cttcagtgcc ggccctgcga ccttgccgga atcggtcctg cgccaggcgc aggcggaaat gctggactgg cacgggtccg gcgcttcgat cgtggaaatg agccatcgcg gcgcggaatt catgtccgtg gctgccgaag ccgaggccga cctgcgtcgc ctgctcgaca tccccgacga ctatgcggtg ctgttcctgt ccggtggcgc caccacccag caggcactga tcccgctcaa cttcgccgcc ccgggccagc gtgccgatta cgtggttagt ggccactggg gcaagacggc ggtcaagcag gccggggtct acgtcgacgt caacattgcc gccagcagcg aggccaacgg ctaccgcgag ctgccggcgc gtgccgactg gcagctgtcg cgcgatgccg cctacgtgca catcaccgcc aacgagacca tccacggcgt cgaattccgt gatgtgcccg acaccggcaa cgtcccgctg atcgccgact tcagctcgtc catcgccagc gaaccgctgg atgtgcgccg ctatggcgtg atctacgccg gcgcgcagaa gaacctgggc ccggtcggcg tcgcggtgat gatcatccgc cgcgacctgc tcgagcgcag tggccagccg cgcgcggaca tcttcgatta ccgctcgcac gtcgcccgcg attcgatgct caacacgccg ccgacctgga actggtatct ggccggcctg gtgttcaagt ggatgctggc cgaaggcggc gtgaccgaat tcgccaagcg caacgccgcc aaggccgcgc tggtgtacgg cgccatcgac ggctccggcg gcttctaccg caatgaagtc gcatatgcgg cccgttcgcg catgaacatt ccgttcttcc tgcccgatgc cgagcttgac gcccgcttcg tcgccgaagc caaggccgct ggcctgctgg cgctgaaggg ccacaaggtg gtcggcggca tccgggcttc gctgtacaac gccatgccgc tggccggggc cgaggcgctg gtcgccttca tggccgattt ccagcagcgt cacggttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for StmaA.00980.a.B1.PW38764
| PURIFICATION DATe: | 12/5/2019 |
| CONCENTRATION: | 51.4mg/ml |
| OBSERVED MW: | 38kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 44 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT XXXXXXXXPX TXXXXVXRXX XXXMXXXXXS XASIXXMXXL XEXFXXVAAX AXXXLRXXXX IPXXYXXLXL SGGAXTXXXX XXXXXXXXXX XXXXXXXXHW XKTAVKQXXV SXDVNIAAXX EAXXXXEXXX RXXXXXSHDX XXVHIXANEX IXXVEFRDXX DTXXVPXIXX XXSSIXSXPL XVRRYXXIYA GXQKNXXXVX VXXXIXXRXL XEXSXXPRXD IXXXXXXVXX XXMXXXPXXW XWYLXXXVFK XXLXXXGVXE XXXXNAXXAX XVYGAIXXXX XXXXNEXAXX XRXRMXXXXF XXXXELXXRX XXXAKAXXXX XLKAHKXVXX IXXSLXNXXX XXXAEALVAX XXXXQQRXX
Validated NT Sequence
atggctcacc accaccacca ccatatgacs mkcrysytcm acwtcrkygy cgsccckknn acctygcmgg amtmsgtcst gcgccangng canrnnnaaa tgcwskaytg snacsggtcc gsngcktcna ttnagnraat gannywycts gnngagkaat tcmtgtmsgt ggctgccsaa gccnaggycr acctgcgwyg scwgmtcsac atccccsann actatgmsgn gctgktcctg tccggyggsg ccrccacynn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nrgccactgg gncaagacgg cggtcaagca ggnngnggtc tccntcgacg tcaacattgc cgccagsagn gaggccancg rstanngcga gntgcnggng cgtrccnact rgmagywgtc gcacgatrny rcttangtgc acatmnnngc saacgagayc atccncgkcg tngaattccg tgatnnrnsn gacaccrgca rcgtnccgnt gatcrccgan ntcagntcgt ccatcnccag yganccgctg gakgtrcgyc gmtatsrcgw ratytaygcc ggcrsgcaga agaacntsgs cmcggtykgc gtcgmggwga wgatcwtcck ccgcgwcctg mtcgarmrca gygkscakcc gcgcsmggac atctwyrakt mcckcwcgca mgtcncccan sakwcsatgy tcracwcgcc smygmcstgg aantggtaty tgsmsgkcnt ggtgttcaag yggatsytgk csgamrrcgg cgtgrcsgaa ktcrmcaasc nsaacgccgm cawggccgmg ntggtgtacg gcgccatcgw srkctmcgkc gknttnyacy gcaaygaast cgcayaygmg ssccgytssc gcatgrwcmw tsmsktcttc nygycsganr ncgagctwgw cryccgctts rtcgsckamg ccaaggccsy tgrcytsmkg smgytgaagg cccacaaggw ggtcgrmgrc atcsrggsyt cgctgyacaa cgmcakgscs mwggmsgrgg csgaggcsct ggtcgcctts ryggncnnyw nccagcagcg tcassrttaa
Expressed Protein Sequence
MAHHHHHHMT RAFNFSAGPA TLPESVLRQA QAEMLDWHGS GASIVEMSHR GAEFMSVAAE AEADLRRLLD IPDDYAVLFL SGGATTQQAL IPLNFAAPGQ RADYVVSGHW GKTAVKQAGV YVDVNIAASS EANGYRELPA RADWQLSRDA AYVHITANET IHGVEFRDVP DTGNVPLIAD FSSSIASEPL DVRRYGVIYA GAQKNLGPVG VAVMIIRRDL LERSGQPRAD IFDYRSHVAR DSMLNTPPTW NWYLAGLVFK WMLAEGGVTE FAKRNAAKAA LVYGAIDGSG GFYRNEVAYA ARSRMNIPFF LPDAELDARF VAEAKAAGLL ALKGHKVVGG IRASLYNAMP LAGAEALVAF MADFQQRHG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGC GCGCGTTCAA CTTCAGTGCC GGCCCTGCGA CCTTGCCGGA ATCGGTCCTG CGCCAGGCGC AGGCGGAAAT GCTGGACTGG CACGGGTCCG GCGCTTCGAT CGTGGAAATG AGCCATCGCG GCGCGGAATT CATGTCCGTG GCTGCCGAAG CCGAGGCCGA CCTGCGTCGC CTGCTCGACA TCCCCGACGA CTATGCGGTG CTGTTCCTGT CCGGTGGCGC CACCACCCAG CAGGCACTGA TCCCGCTCAA CTTCGCCGCC CCGGGCCAGC GTGCCGATTA CGTGGTTAGT GGCCACTGGG GCAAGACGGC GGTCAAGCAG GCCGGGGTCT ACGTCGACGT CAACATTGCC GCCAGCAGCG AGGCCAACGG CTACCGCGAG CTGCCGGCGC GTGCCGACTG GCAGCTGTCG CGCGATGCCG CCTACGTGCA CATCACCGCC AACGAGACCA TCCACGGCGT CGAATTCCGT GATGTGCCCG ACACCGGCAA CGTCCCGCTG ATCGCCGACT TCAGCTCGTC CATCGCCAGC GAACCGCTGG ATGTGCGCCG CTATGGCGTG ATCTACGCCG GCGCGCAGAA GAACCTGGGC CCGGTCGGCG TCGCGGTGAT GATCATCCGC CGCGACCTGC TCGAGCGCAG TGGCCAGCCG CGCGCGGACA TCTTCGATTA CCGCTCGCAC GTCGCCCGCG ATTCGATGCT CAACACGCCG CCGACCTGGA ACTGGTATCT GGCCGGCCTG GTGTTCAAGT GGATGCTGGC CGAAGGCGGC GTGACCGAAT TCGCCAAGCG CAACGCCGCC AAGGCCGCGC TGGTGTACGG CGCCATCGAC GGCTCCGGCG GCTTCTACCG CAATGAAGTC GCATATGCGG CCCGTTCGCG CATGAACATT CCGTTCTTCC TGCCCGATGC CGAGCTTGAC GCCCGCTTCG TCGCCGAAGC CAAGGCCGCT GGCCTGCTGG CGCTGAAGGG CCACAAGGTG GTCGGCGGCA TCCGGGCTTC GCTGTACAAC GCCATGCCGC TGGCCGGGGC CGAGGCGCTG GTCGCCTTCA TGGCCGATTT CCAGCAGCGT CACGGTTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T