StmaA.00952.a
UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) (murA)
| CENTER ID: | StmaA.00952.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00952.a.B1.GE43273 | Full length(StmaA.00952.a) | 1 | 423 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00952.a.B1.PW38738 | Full length(StmaA.00952.a) | 1 | 423 | ||
| StmaA.00952.a.B1.PW38746 | Full length(StmaA.00952.a) | 1 | 423 |
Structures
6WFM
DEPOSITED: 4/3/2020
DETERMINATION: XRay
CLONE: StmaA.00952.a.B1.GE43273
PROTEIN: StmaA.00952.a.B1.PW38746
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.1082 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKIVVTGGA ALHGEVSISG AKNAVLPILC ATLLADEPVE ITNVPHLHDV VTTVKLLGEL GAKVTIDQGT LSRGSAIVVD PRPVNQHVAP YELVKTMRAS ILVLGPLLAR FGAAEVSLPG GCAIGSRPVD QHIKGLQALG AEIVVENGFI KASAKRLKGG HFTFDMVSVT GTENVLMGAV LAEGTTVLDN CAMEPEVTDL AHCLIALGAK IEGLGTARLV IEGVERLSGG RHEVLPDRIE TGTFLVAAAM TGGKVTVNRA RPNTMDAVLS KLVEAGAKIE TTDDSITLDM QGRRPKAVNL TTAPYPAFPT DMQAQFMALN CVADGVGVIN ETIFENRFMH VNELLRLGAD IQVEGHTAIV RGSEHLSGAP VMATDLRASA SLILAGLMAS GDTTIDRIYH LDRGYENIEE KLSSLGATIR RVP
NT Sequence
ATGGCCAAGA TCGTTGTGAC CGGCGGCGCT GCGCTGCACG GTGAAGTGAG CATCTCCGGC GCCAAGAACG CCGTCCTCCC CATTCTCTGC GCGACCCTGC TGGCCGATGA GCCGGTGGAG ATCACCAACG TGCCGCACCT GCACGACGTG GTCACCACGG TGAAGCTGCT TGGCGAGCTG GGCGCCAAGG TCACCATCGA CCAGGGCACG CTGTCGCGCG GCAGCGCGAT CGTGGTCGAC CCGCGTCCGG TCAACCAGCA CGTCGCACCG TATGAGCTGG TCAAGACCAT GCGCGCCTCG ATCCTGGTGC TGGGCCCGCT GCTGGCCCGC TTCGGCGCCG CCGAAGTGTC GCTGCCGGGC GGCTGCGCGA TCGGCTCGCG ACCGGTCGAC CAGCACATCA AGGGCCTGCA GGCGCTGGGC GCCGAGATCG TGGTCGAGAA CGGCTTCATC AAGGCCAGCG CCAAGCGCCT GAAGGGTGGC CACTTCACCT TCGACATGGT CAGCGTCACC GGCACCGAGA ACGTGCTGAT GGGCGCGGTG CTGGCTGAAG GGACCACCGT CCTCGACAAC TGCGCGATGG AACCGGAAGT GACCGACCTG GCGCACTGCC TGATCGCGCT GGGCGCGAAG ATCGAAGGCC TGGGCACCGC CCGCCTGGTG ATCGAGGGCG TCGAGCGCCT GTCCGGTGGC CGCCACGAAG TGCTGCCCGA CCGCATCGAG ACCGGCACCT TCCTGGTGGC CGCGGCGATG ACCGGTGGCA AGGTCACGGT CAACCGTGCG CGCCCGAACA CCATGGACGC GGTGCTGTCC AAGCTGGTCG AGGCCGGTGC GAAGATCGAG ACCACCGACG ACAGCATCAC CCTGGACATG CAGGGCAGGC GGCCGAAGGC GGTCAACCTG ACCACCGCGC CGTACCCGGC ATTCCCGACC GACATGCAGG CGCAGTTCAT GGCACTCAAC TGCGTGGCCG ATGGCGTGGG CGTGATCAAC GAAACGATCT TCGAGAACCG TTTCATGCAC GTCAACGAAC TGCTGCGCCT GGGCGCGGAC ATCCAGGTTG AAGGCCACAC TGCCATCGTG CGTGGCAGCG AGCACCTGAG CGGCGCACCG GTGATGGCGA CCGACCTGCG TGCGTCGGCC TCGCTGATCC TGGCCGGCCT GATGGCCAGC GGCGATACCA CCATCGACCG CATCTACCAC CTTGATCGCG GCTACGAGAA CATCGAAGAG AAGCTGTCTT CGCTCGGCGC CACCATCCGG CGCGTGCCA
Details for StmaA.00952.a.B1.GE43273
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA KIVVTGGAAL HGEVSISGAK NAVLPILCAT LLADEPVEIT NVPHLHDVVT TVKLLGELGA KVTIDQGTLS RGSAIVVDPR PVNQHVAPYE LVKTMRASIL VLGPLLARFG AAEVSLPGGC AIGSRPVDQH IKGLQALGAE IVVENGFIKA SAKRLKGGHF TFDMVSVTGT ENVLMGAVLA EGTTVLDNCA MEPEVTDLAH CLIALGAKIE GLGTARLVIE GVERLSGGRH EVLPDRIETG TFLVAAAMTG GKVTVNRARP NTMDAVLSKL VEAGAKIETT DDSITLDMQG RRPKAVNLTT APYPAFPTDM QAQFMALNCV ADGVGVINET IFENRFMHVN ELLRLGADIQ VEGHTAIVRG SEHLSGAPVM ATDLRASASL ILAGLMASGD TTIDRIYHLD RGYENIEEKL SSLGATIRRV P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca agatcgttgt gaccggcggc gctgcgctgc acggtgaagt gagcatctcc ggcgccaaga acgccgtcct ccccattctc tgcgcgaccc tgctggccga tgagccggtg gagatcacca acgtgccgca cctgcacgac gtggtcacca cggtgaagct gcttggcgag ctgggcgcca aggtcaccat cgaccagggc acgctgtcgc gcggcagcgc gatcgtggtc gacccgcgtc cggtcaacca gcacgtcgca ccgtatgagc tggtcaagac catgcgcgcc tcgatcctgg tgctgggccc gctgctggcc cgcttcggcg ccgccgaagt gtcgctgccg ggcggctgcg cgatcggctc gcgaccggtc gaccagcaca tcaagggcct gcaggcgctg ggcgccgaga tcgtggtcga gaacggcttc atcaaggcca gcgccaagcg cctgaagggt ggccacttca ccttcgacat ggtcagcgtc accggcaccg agaacgtgct gatgggcgcg gtgctggctg aagggaccac cgtcctcgac aactgcgcga tggaaccgga agtgaccgac ctggcgcact gcctgatcgc gctgggcgcg aagatcgaag gcctgggcac cgcccgcctg gtgatcgagg gcgtcgagcg cctgtccggt ggccgccacg aagtgctgcc cgaccgcatc gagaccggca ccttcctggt ggccgcggcg atgaccggtg gcaaggtcac ggtcaaccgt gcgcgcccga acaccatgga cgcggtgctg tccaagctgg tcgaggccgg tgcgaagatc gagaccaccg acgacagcat caccctggac atgcagggca ggcggccgaa ggcggtcaac ctgaccaccg cgccgtaccc ggcattcccg accgacatgc aggcgcagtt catggcactc aactgcgtgg ccgatggcgt gggcgtgatc aacgaaacga tcttcgagaa ccgtttcatg cacgtcaacg aactgctgcg cctgggcgcg gacatccagg ttgaaggcca cactgccatc gtgcgtggca gcgagcacct gagcggcgca ccggtgatgg cgaccgacct gcgtgcgtcg gcctcgctga tcctggccgg cctgatggcc agcggcgata ccaccatcga ccgcatctac caccttgatc gcggctacga gaacatcgaa gagaagctgt cttcgctcgg cgccaccatc cggcgcgtgc catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for StmaA.00952.a.B1.PW38738
| PURIFICATION DATe: | 10/22/2019 |
| CONCENTRATION: | 13mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA KIVVTGGAAL HGEVSISGAK NAVLPILCAT LLADEPVEIT NVPHLHDVVT TVKLLGELGA KVTIDQGTLS RGSAIVVDPR PVNQHVAPYE LVKTMRASIL VLGPLLARFG AAEVSLPGGC AIGSRPVDQH IKGLQALGAE IVVENGFIKA SAKRLKGGHF TFDMVSVTGT ENVLMGAVLA EGTTVLDNCA MEPEVTDLAH CLIALGAKIE GLGTARLVIE GVERLSGGRH EVLPDRIETG TFLVAAAMTG GKVTVNRARP NTMDAVLSKL VEAGAKIETT DDSITLDMQG RRPKAVNLTT APYPAFPTDM QAQFMALNCV ADGVGVINET IFENRFMHVN ELLRLGADIQ VEGHTAIVRG SEHLSGAPVM ATDLRASASL ILAGLMASGD TTIDRIYHLD RGYENIEEKL SSLGATIRRV P
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA AGATCGTTGT GACCGGCGGC GCTGCGCTGC ACGGTGAAGT GAGCATCTCC GGCGCCAAGA ACGCCGTCCT CCCCATTCTC TGCGCGACCC TGCTGGCCGA TGAGCCGGTG GAGATCACCA ACGTGCCGCA CCTGCACGAC GTGGTCACCA CGGTGAAGCT GCTTGGCGAG CTGGGCGCCA AGGTCACCAT CGACCAGGGC ACGCTGTCGC GCGGCAGCGC GATCGTGGTC GACCCGCGTC CGGTCAACCA GCACGTCGCA CCGTATGAGC TGGTCAAGAC CATGCGCGCC TCGATCCTGG TGCTGGGCCC GCTGCTGGCC CGCTTCGGCG CCGCCGAAGT GTCGCTGCCG GGCGGCTGCG CGATCGGCTC GCGACCGGTC GACCAGCACA TCAAGGGCCT GCAGGCGCTG GGCGCCGAGA TCGTGGTCGA GAACGGCTTC ATCAAGGCCA GCGCCAAGCG CCTGAAGGGT GGCCACTTCA CCTTCGACAT GGTCAGCGTC ACCGGCACCG AGAACGTGCT GATGGGCGCG GTGCTGGCTG AAGGGACCAC CGTCCTCGAC AACTGCGCGA TGGAACCGGA AGTGACCGAC CTGGCGCACT GCCTGATCGC GCTGGGCGCG AAGATCGAAG GCCTGGGCAC CGCCCGCCTG GTGATCGAGG GCGTCGAGCG CCTGTCCGGT GGCCGCCACG AAGTGCTGCC CGACCGCATC GAGACCGGCA CCTTCCTGGT GGCCGCGGCG ATGACCGGTG GCAAGGTCAC GGTCAACCGT GCGCGCCCGA ACACCATGGA CGCGGTGCTG TCCAAGCTGG TCGAGGCCGG TGCGAAGATC GAGACCACCG ACGACAGCAT CACCCTGGAC ATGCAGGGCA GGCGGCCGAA GGCGGTCAAC CTGACCACCG CGCCGTACCC GGCATTCCCG ACCGACATGC AGGCGCAGTT CATGGCACTC AACTGCGTGG CCGATGGCGT GGGCGTGATC AACGAAACGA TCTTCGAGAA CCGTTTCATG CACGTCAACG AACTGCTGCG CCTGGGCGCG GACATCCAGG TTGAAGGCCA CACTGCCATC GTGCGTGGCA GCGAGCACCT GAGCGGCGCA CCGGTGATGG CGACCGACCT GCGTGCGTCG GCCTCGCTGA TCCTGGCCGG CCTGATGGCC AGCGGCGATA CCACCATCGA CCGCATCTAC CACCTTGATC GCGGCTACGA GAACATCGAA GAGAAGCTGT CTTCGCTCGG CGCCACCATC CGGCGCGTGC CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT
Details for StmaA.00952.a.B1.PW38746
| PURIFICATION DATe: | 10/31/2019 |
| CONCENTRATION: | 9mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 10µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMA KIVVTGGAAL HGEVSISGAK NAVLPILCAT LLADEPVEIT NVPHLHDVVT TVKLLGELGA KVTIDQGTLS RGSAIVVDPR PVNQHVAPYE LVKTMRASIL VLGPLLARFG AAEVSLPGGC AIGSRPVDQH IKGLQALGAE IVVENGFIKA SAKRLKGGHF TFDMVSVTGT ENVLMGAVLA EGTTVLDNCA MEPEVTDLAH CLIALGAKIE GLGTARLVIE GVERLSGGRH EVLPDRIETG TFLVAAAMTG GKVTVNRARP NTMDAVLSKL VEAGAKIETT DDSITLDMQG RRPKAVNLTT APYPAFPTDM QAQFMALNCV ADGVGVINET IFENRFMHVN ELLRLGADIQ VEGHTAIVRG SEHLSGAPVM ATDLRASASL ILAGLMASGD TTIDRIYHLD RGYENIEEKL SSLGATIRRV P
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA AGATCGTTGT GACCGGCGGC GCTGCGCTGC ACGGTGAAGT GAGCATCTCC GGCGCCAAGA ACGCCGTCCT CCCCATTCTC TGCGCGACCC TGCTGGCCGA TGAGCCGGTG GAGATCACCA ACGTGCCGCA CCTGCACGAC GTGGTCACCA CGGTGAAGCT GCTTGGCGAG CTGGGCGCCA AGGTCACCAT CGACCAGGGC ACGCTGTCGC GCGGCAGCGC GATCGTGGTC GACCCGCGTC CGGTCAACCA GCACGTCGCA CCGTATGAGC TGGTCAAGAC CATGCGCGCC TCGATCCTGG TGCTGGGCCC GCTGCTGGCC CGCTTCGGCG CCGCCGAAGT GTCGCTGCCG GGCGGCTGCG CGATCGGCTC GCGACCGGTC GACCAGCACA TCAAGGGCCT GCAGGCGCTG GGCGCCGAGA TCGTGGTCGA GAACGGCTTC ATCAAGGCCA GCGCCAAGCG CCTGAAGGGT GGCCACTTCA CCTTCGACAT GGTCAGCGTC ACCGGCACCG AGAACGTGCT GATGGGCGCG GTGCTGGCTG AAGGGACCAC CGTCCTCGAC AACTGCGCGA TGGAACCGGA AGTGACCGAC CTGGCGCACT GCCTGATCGC GCTGGGCGCG AAGATCGAAG GCCTGGGCAC CGCCCGCCTG GTGATCGAGG GCGTCGAGCG CCTGTCCGGT GGCCGCCACG AAGTGCTGCC CGACCGCATC GAGACCGGCA CCTTCCTGGT GGCCGCGGCG ATGACCGGTG GCAAGGTCAC GGTCAACCGT GCGCGCCCGA ACACCATGGA CGCGGTGCTG TCCAAGCTGG TCGAGGCCGG TGCGAAGATC GAGACCACCG ACGACAGCAT CACCCTGGAC ATGCAGGGCA GGCGGCCGAA GGCGGTCAAC CTGACCACCG CGCCGTACCC GGCATTCCCG ACCGACATGC AGGCGCAGTT CATGGCACTC AACTGCGTGG CCGATGGCGT GGGCGTGATC AACGAAACGA TCTTCGAGAA CCGTTTCATG CACGTCAACG AACTGCTGCG CCTGGGCGCG GACATCCAGG TTGAAGGCCA CACTGCCATC GTGCGTGGCA GCGAGCACCT GAGCGGCGCA CCGGTGATGG CGACCGACCT GCGTGCGTCG GCCTCGCTGA TCCTGGCCGG CCTGATGGCC AGCGGCGATA CCACCATCGA CCGCATCTAC CACCTTGATC GCGGCTACGA GAACATCGAA GAGAAGCTGT CTTCGCTCGG CGCCACCATC CGGCGCGTGC CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT