SSGCID: Target Page

StmaA.00948.a

Ribosome recycling factor (frr)

CENTER ID:	StmaA.00948.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00948.a.B1.GE43272	Full length(StmaA.00948.a)		1	184

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.1440

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLNDIKNNAQ TRMAKSIDAL KHTLTSIRTG RATPALLDRV TVNAYGNAST PLNQVASISN ADAHSLLVTP FDKGMIKEIE KGLYNAEFTP NTLGTAIRIN MPPPTEERRK ELVKQVQKEG EGAKIAIRNI RQDANKEIAK LVKDKAISED EKKRGEDDIQ KLTDTNIKDV DKVVADKEKE LLSV

NT Sequence

ATGCTCAACG ACATCAAGAA CAACGCGCAG ACGCGCATGG CCAAGAGCAT CGACGCTCTG AAGCACACCC TCACCTCCAT CCGGACCGGG CGTGCCACGC CGGCCCTGCT GGACCGCGTG ACGGTCAATG CCTACGGCAA CGCCAGCACC CCGCTGAACC AGGTTGCCTC GATCTCCAAC GCCGATGCGC ACTCGCTGCT GGTCACCCCG TTCGACAAGG GCATGATCAA GGAGATCGAG AAGGGTCTCT ACAACGCCGA GTTCACCCCG AACACGCTGG GCACCGCGAT CCGCATCAAC ATGCCGCCGC CGACCGAAGA GCGCCGCAAG GAGCTGGTCA AGCAGGTGCA GAAGGAAGGC GAAGGCGCCA AGATCGCGAT CCGCAACATC CGTCAGGACG CGAACAAGGA AATCGCCAAG CTGGTCAAGG ACAAGGCGAT CAGCGAGGAC GAGAAGAAGC GCGGTGAGGA CGACATCCAG AAGTTGACCG ACACCAACAT CAAGGACGTC GACAAGGTCG TTGCCGACAA GGAAAAAGAA CTGCTGTCGG TC

Details for StmaA.00948.a.B1.GE43272

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/18/2019
EXPECTED MW:	21kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	82
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMV NDIKNNAQTR MXKXIXALKH TLTSIRXGRA TPALLDRXTV NAYXNXSTPL XXVASISXAX XXSLLXXPXD KGMIKEIEKG XXNAEFXPNT LGTAIRINMP XXTEERRKXL XXQVQKEGEG XXIAIRNIRQ DAXKEIAKLV KDKAIXEDEX XXGEDDIQKL TDTNIKDVDK VVADXEKELL X

Validated NT Sequence

accnacagca gttctttttc nnngtcggcn acgaccttgt cgacgtcctt gatgttggtg tcggtcaact tctggatgtc gtcctcaccg nnnnncntct cgtcctcnnt gatcgccttg tccttgacca gcttggcgat ttccttgntc gcgtcctgac ggatgttgcg gatcgcgatc tngnnncctt cnccttcctt ctgcacctgn ttgnccagnt ccttgcggcg ctcttcggtc nnnnncggca tgttgatgcg gatcgcggtg cccagcgtgt tnggggngaa ctcggcgttg nngngaccct tctcgatctc cttgatcatg cccttgtcna acggggngnc cagcagcgag tnnncntnng cgtnggagat cgaggcaacn ngnttcagcg gggtgctggn gttgncgtag gcattgaccg tcncncggtc cagcagggcc ggcgtggcac gcccngnccg gatggaggtg agggtgtgct tcagagcgnc gatgntcttg nncatgcgcg tctgcgcgtt gttcttgatg tcgttgacca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHML NDIKNNAQTR MAKSIDALKH TLTSIRTGRA TPALLDRVTV NAYGNASTPL NQVASISNAD AHSLLVTPFD KGMIKEIEKG LYNAEFTPNT LGTAIRINMP PPTEERRKEL VKQVQKEGEG AKIAIRNIRQ DANKEIAKLV KDKAISEDEK KRGEDDIQKL TDTNIKDVDK VVADKEKELL SV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctca acgacatcaa gaacaacgcg cagacgcgca tggccaagag catcgacgct ctgaagcaca ccctcacctc catccggacc gggcgtgcca cgccggccct gctggaccgc gtgacggtca atgcctacgg caacgccagc accccgctga accaggttgc ctcgatctcc aacgccgatg cgcactcgct gctggtcacc ccgttcgaca agggcatgat caaggagatc gagaagggtc tctacaacgc cgagttcacc ccgaacacgc tgggcaccgc gatccgcatc aacatgccgc cgccgaccga agagcgccgc aaggagctgg tcaagcaggt gcagaaggaa ggcgaaggcg ccaagatcgc gatccgcaac atccgtcagg acgcgaacaa ggaaatcgcc aagctggtca aggacaaggc gatcagcgag gacgagaaga agcgcggtga ggacgacatc cagaagttga ccgacaccaa catcaaggac gtcgacaagg tcgttgccga caaggaaaaa gaactgctgt cggtctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat