StmaA.00945.a

Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) (carA)

CENTER ID: StmaA.00945.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00945.a.B1.GE43271 Full length(StmaA.00945.a) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.2111

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTQAAILVLE DGTVFEGESV GAPGLSVGEV VFNTAMTGYQ EVLTDPSYAR QMVTLTYPHI GNTGMTDQDN EASKVWSAGL IVRDVPRRPS NWRSQVSLQD WLIQRGVVAI AGIDTRKLTR ILREKGAQNG ALMAGDIDVE KALEAARKFP GLKGMDLAKV VTTEKTYTWT EGQLDLDANA FVSVPAKYKV VAYDFGVKTN ILRMLAERGC EVTVVPAQTP AAEVLALKPD GVFLSNGPGD PEPCDYAIEA IKTFIDVKIP TFGICLGHQL LGLASGAQTM KMGHGHHGAN HPVQDLDSGR VMITSQNHGF AIDEATLPPT LRVTHRSLFD GTNQGVARTD VPAFSFQGHP EASPGPTDVG PLFDRFVVLM EQAKA
NT Sequence
GTGACCCAAG CCGCAATCCT CGTCCTCGAA GACGGCACCG TATTCGAGGG CGAATCCGTA GGCGCGCCCG GCCTGTCCGT CGGTGAGGTG GTGTTCAACA CCGCCATGAC CGGCTACCAG GAAGTGCTGA CCGACCCGTC CTACGCCCGG CAGATGGTCA CGCTGACCTA TCCGCATATC GGCAACACCG GCATGACCGA CCAGGACAAT GAAGCGTCCA AGGTGTGGTC GGCCGGCCTG ATCGTGCGCG ATGTTCCGCG TCGCCCCAGC AACTGGCGCA GCCAGGTGTC GCTGCAGGAC TGGCTGATCC AGCGTGGCGT GGTTGCCATC GCCGGCATCG ACACCCGCAA GCTGACCCGC ATCCTGCGCG AGAAGGGCGC GCAGAACGGT GCGCTGATGG CCGGCGACAT CGACGTGGAA AAGGCACTGG AAGCCGCCCG CAAGTTCCCG GGGCTGAAGG GCATGGATCT GGCCAAGGTC GTCACTACCG AGAAGACCTA CACCTGGACC GAGGGCCAGC TGGACCTGGA TGCCAATGCC TTCGTCAGCG TGCCGGCCAA GTACAAGGTC GTGGCCTACG ATTTCGGCGT GAAGACCAAC ATCCTGCGCA TGCTGGCCGA GCGCGGCTGC GAAGTGACCG TGGTGCCGGC ACAGACCCCG GCCGCCGAAG TGCTGGCGCT GAAGCCGGAT GGCGTGTTCC TGTCCAACGG CCCGGGTGAC CCGGAACCGT GCGACTACGC CATCGAAGCG ATCAAGACCT TCATCGACGT GAAGATCCCG ACCTTCGGCA TCTGCCTGGG CCACCAGCTG CTGGGCCTGG CCTCGGGCGC GCAGACCATG AAGATGGGCC ACGGCCACCA CGGTGCCAAC CACCCGGTGC AGGACCTGGA CAGCGGCCGG GTGATGATCA CCTCGCAGAA CCACGGCTTC GCGATCGATG AAGCGACCCT GCCGCCGACC CTGCGCGTGA CCCACCGCTC GCTGTTCGAT GGCACCAACC AGGGTGTCGC CCGCACCGAC GTGCCGGCGT TCTCGTTCCA GGGCCATCCG GAAGCGTCGC CGGGCCCGAC CGATGTCGGC CCGCTGTTCG ACCGCTTCGT GGTGCTGATG GAGCAGGCCA AGGCG
Details for StmaA.00945.a.B1.GE43271
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 52
PERCENT COVERAGE: 64
Validated AA Sequence
MAHHHHHHMV TQAAILVLED GTVFEGESVG APGLSVGEVV FNTAMTGXXE VLTDPSYARQ MVTLTYPHIG NTGMTDQDNE ASKVWSXRPD RARCSASPQQ LAQPGVAAGL ADPAWRGCHR RHRHPQADPH PAREGRAERC ADGRRHRRGK GTGSRPQVPG AEGHGSGQGR HYREDLHLDR GPAGPGCQCL RQRAGQVQGR GLRFRREDQH PAHAGRARLR SDRGAGTDPG RRSAGAEAGW RVPVQRPG
Validated NT Sequence
aagcggtcga acagcgggcc gacatcggtc gggcccggcg acgcttccgg atggccctgg aacgagaacg ccggcacgtc ggtgcgggcg acaccctggt tggtgccatc gaacagcgag cggtgggtca cgcgcagggt cggcggcagg gtcgcttcat cgatcgcgaa gccgtggttc tgcgaggtga tcatcacccg gccgctgtcc aggtcctgca ccgggtggtt ggcaccgtgg tggccgtggc ccatcttcat ggtctgcgcg cccgaggcca ggcccagcag ctggtggccc aggcagatgc cgaaggtcgg gatcttcacg tcgatgaagg tcttgatcgc ttcgatggcg tagtcgcacg gttccgggtc acccgggccg ttggacagga acacgccatc cggcttcagc gccagcactt cggcggccgg ggtctgtgcc ggcaccacgg tcacttcgca gccgcgctcg gccagcatgc gcaggatgtt ggtcttcacg ccgaaatcgt aggccacgac cttgtacttg gccggcacgc tgacgaaggc attggcatcc aggtccagct ggccctcggt ccaggtgtag gtcttctcgg tagtgacgac cttggccaga tccatgccct tcagccccgg gaacttgcgg gcggcttcca gtgccttttc cacgtcgatg tcgccggcca tcagcgcacc gttctgcgcg cccttctcgc gcaggatgcg ggtcagcttg cgggtgtcga tgccggcgat ggcaaccacg ccacgctgga tcagccagtc ctgcagcgac acctggctgc gccagttgct ggggcgacgc ggaacatcgc gcacgatcag gccggnccga ccacaccttg gacgcttcat tgtcctggtc ggtcatgccg gtgttgccga tatgcggata ggtcagcgtg accatctgcc gggcgtagga cgggtcggtc agcacttccn nnnnnccggt catggcggtg ttgaacacca cctcaccgac ggacaggccg ggcgcgccta cggattcgcc ctcgaatacg gtnccgtctt cgaggacgag gattgcggct tgggtcacca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMT QAAILVLEDG TVFEGESVGA PGLSVGEVVF NTAMTGYQEV LTDPSYARQM VTLTYPHIGN TGMTDQDNEA SKVWSAGLIV RDVPRRPSNW RSQVSLQDWL IQRGVVAIAG IDTRKLTRIL REKGAQNGAL MAGDIDVEKA LEAARKFPGL KGMDLAKVVT TEKTYTWTEG QLDLDANAFV SVPAKYKVVA YDFGVKTNIL RMLAERGCEV TVVPAQTPAA EVLALKPDGV FLSNGPGDPE PCDYAIEAIK TFIDVKIPTF GICLGHQLLG LASGAQTMKM GHGHHGANHP VQDLDSGRVM ITSQNHGFAI DEATLPPTLR VTHRSLFDGT NQGVARTDVP AFSFQGHPEA SPGPTDVGPL FDRFVVLMEQ AKA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaccc aagccgcaat cctcgtcctc gaagacggca ccgtattcga gggcgaatcc gtaggcgcgc ccggcctgtc cgtcggtgag gtggtgttca acaccgccat gaccggctac caggaagtgc tgaccgaccc gtcctacgcc cggcagatgg tcacgctgac ctatccgcat atcggcaaca ccggcatgac cgaccaggac aatgaagcgt ccaaggtgtg gtcggccggc ctgatcgtgc gcgatgttcc gcgtcgcccc agcaactggc gcagccaggt gtcgctgcag gactggctga tccagcgtgg cgtggttgcc atcgccggca tcgacacccg caagctgacc cgcatcctgc gcgagaaggg cgcgcagaac ggtgcgctga tggccggcga catcgacgtg gaaaaggcac tggaagccgc ccgcaagttc ccggggctga agggcatgga tctggccaag gtcgtcacta ccgagaagac ctacacctgg accgagggcc agctggacct ggatgccaat gccttcgtca gcgtgccggc caagtacaag gtcgtggcct acgatttcgg cgtgaagacc aacatcctgc gcatgctggc cgagcgcggc tgcgaagtga ccgtggtgcc ggcacagacc ccggccgccg aagtgctggc gctgaagccg gatggcgtgt tcctgtccaa cggcccgggt gacccggaac cgtgcgacta cgccatcgaa gcgatcaaga ccttcatcga cgtgaagatc ccgaccttcg gcatctgcct gggccaccag ctgctgggcc tggcctcggg cgcgcagacc atgaagatgg gccacggcca ccacggtgcc aaccacccgg tgcaggacct ggacagcggc cgggtgatga tcacctcgca gaaccacggc ttcgcgatcg atgaagcgac cctgccgccg accctgcgcg tgacccaccg ctcgctgttc gatggcacca accagggtgt cgcccgcacc gacgtgccgg cgttctcgtt ccagggccat ccggaagcgt cgccgggccc gaccgatgtc ggcccgctgt tcgaccgctt cgtggtgctg atggagcagg ccaaggcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat