StmaA.00944.a
Ribose-5-phosphate isomerase A (EC 5.3.1.6) (rpiA)
| CENTER ID: | StmaA.00944.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00944.a.B1.GE43270 | Full length(StmaA.00944.a) | 1 | 215 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00944.a.B1.PW38722 | Full length(StmaA.00944.a) | 1 | 215 |
Structures
7LDA
DEPOSITED: 1/13/2021
DETERMINATION: XRay
CLONE: StmaA.00944.a.B1.GE43270
PROTEIN: StmaA.00944.a.B1.PW38722
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3643 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEAKRLAAE KAIEYVEDGM IVGVGTGSTV AYFIDALARI QHRIKGAVSS SEQSTARLKQ HGIEVIELNH SGNLSLYVDG ADECDANKCL IKGGGAALTR EKIIAEASER FICIIDPSKQ VPVLGRFPLP VEVIPMARSL VARQIRDMTG GQPTWREGVV TDNGNQILDI HNLQITDPEK LERELNQLPG VVCVGLFARR RADVVIVGGE PPVVL
NT Sequence
ATGTCCGAAG CCAAGCGCCT GGCCGCCGAG AAAGCCATCG AGTACGTTGA AGACGGCATG ATCGTCGGTG TCGGCACCGG TTCCACCGTG GCCTATTTCA TCGATGCCCT GGCCCGCATC CAGCACCGCA TCAAGGGCGC TGTTTCCAGC TCCGAGCAGA GCACCGCGCG CCTGAAGCAG CACGGCATCG AGGTGATCGA GCTGAACCAC AGCGGCAACC TGTCGCTGTA CGTCGATGGC GCCGATGAGT GCGACGCCAA CAAGTGCCTG ATCAAGGGCG GCGGCGCCGC GCTGACCCGC GAGAAGATCA TCGCCGAGGC CAGCGAGCGC TTCATCTGCA TCATCGACCC GAGCAAGCAG GTGCCGGTGC TGGGCAGGTT CCCGCTGCCG GTGGAAGTGA TCCCGATGGC GCGCAGCCTG GTCGCCCGCC AGATCCGCGA CATGACCGGC GGCCAGCCGA CCTGGCGTGA AGGCGTGGTC ACCGACAACG GCAACCAGAT CCTGGACATC CACAACCTGC AGATCACCGA TCCGGAAAAG CTGGAGCGCG AGCTCAACCA GCTGCCGGGC GTGGTCTGCG TCGGCCTGTT CGCCCGCCGC CGCGCCGATG TGGTGATCGT CGGCGGCGAG CCGCCGGTCG TGCTC
Details for StmaA.00944.a.B1.GE43270
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 24kDa |
| OBSERVED MW: | 25kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS EAKRLAAEKA IEYVEDGMIV GVGTGSTVAY FIDALARIQH RIKGAVSSSE QSTARLKQHG IEVIELNHSG NLSLYVDGAD ECDANKCLIK GGGAALTREK IIAEASERFI CIIDPSKQVP VLGRFPLPVE VIPMARSLVA RQIRDMTGGQ PTWREGVVTD NGNQILDIHN LQITDPEKLE RELNQLPGVV CVGLFARRRA DVVIVGGEPP VVL
Validated NT Sequence
atcttactca cttagagcac gaccggcggc tcgccgccga cgatcaccac atcggcgcgg cggcgggcga acaggccgac gcagaccacg cccggcagct ggttgagctc gcgctccagc ttttccggat cggtgatctg caggttgtgg atgtccagga tctggttgcc gttgtcggtg accacgcctt cacgccaggt cggctggccg ccggtcatgt cgcggatctg gcgggcgacc aggctgcgcg ccatcgggat cacttccacc ggcagcggga acctgcccag caccggcacc tgcttgctcg ggtcgatgat gcagatgaag cgctcgctgg cctcggcgat gatcttctcg cgggtcagcg cggcgccgcc gcccttgatc aggcacttgt tggcgtcgca ctcatcggcg ccatcgacgt acagcgacag gttgccgctg tggttcagct cgatcacctc gatgccgtgc tgcttcaggc gcgcggtgct ctgctcggag ctggaaacag cgcccttgat gcggtgctgg atgcgggcca gggcatcgat gaaataggcc acggtggaac cggtgccgac accgacgatc atgccgtctt caacgtactc gatggctttc tcggcggcca ggcgcttggc ttcggacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS EAKRLAAEKA IEYVEDGMIV GVGTGSTVAY FIDALARIQH RIKGAVSSSE QSTARLKQHG IEVIELNHSG NLSLYVDGAD ECDANKCLIK GGGAALTREK IIAEASERFI CIIDPSKQVP VLGRFPLPVE VIPMARSLVA RQIRDMTGGQ PTWREGVVTD NGNQILDIHN LQITDPEKLE RELNQLPGVV CVGLFARRRA DVVIVGGEPP VVL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg aagccaagcg cctggccgcc gagaaagcca tcgagtacgt tgaagacggc atgatcgtcg gtgtcggcac cggttccacc gtggcctatt tcatcgatgc cctggcccgc atccagcacc gcatcaaggg cgctgtttcc agctccgagc agagcaccgc gcgcctgaag cagcacggca tcgaggtgat cgagctgaac cacagcggca acctgtcgct gtacgtcgat ggcgccgatg agtgcgacgc caacaagtgc ctgatcaagg gcggcggcgc cgcgctgacc cgcgagaaga tcatcgccga ggccagcgag cgcttcatct gcatcatcga cccgagcaag caggtgccgg tgctgggcag gttcccgctg ccggtggaag tgatcccgat ggcgcgcagc ctggtcgccc gccagatccg cgacatgacc ggcggccagc cgacctggcg tgaaggcgtg gtcaccgaca acggcaacca gatcctggac atccacaacc tgcagatcac cgatccggaa aagctggagc gcgagctcaa ccagctgccg ggcgtggtct gcgtcggcct gttcgcccgc cgccgcgccg atgtggtgat cgtcggcggc gagccgccgg tcgtgctctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for StmaA.00944.a.B1.PW38722
| PURIFICATION DATe: | 10/17/2019 |
| CONCENTRATION: | 29.2mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS EAKRLAAEKA IEYVEDGMIV GVGTGSTVAY FIDALARIQH RIKGAVSSSE QSTARLKQHG IEVIELNHSG NLSLYVDGAD ECDANKCLIK GGGAALTREK IIAEASERFI CIIDPSKQVP VLGRFPLPVE VIPMARSLVA RQIRDMTGGQ PTWREGVVTD NGNQILDIHN LQITDPEKLE RELNQLPGVV CVGLFARRRA DVVIVGGEPP VVL
Validated NT Sequence
atcttactca cttagagcac gaccggcggc tcgccgccga cgatcaccac atcggcgcgg cggcgggcga acaggccgac gcagaccacg cccggcagct ggttgagctc gcgctccagc ttttccggat cggtgatctg caggttgtgg atgtccagga tctggttgcc gttgtcggtg accacgcctt cacgccaggt cggctggccg ccggtcatgt cgcggatctg gcgggcgacc aggctgcgcg ccatcgggat cacttccacc ggcagcggga acctgcccag caccggcacc tgcttgctcg ggtcgatgat gcagatgaag cgctcgctgg cctcggcgat gatcttctcg cgggtcagcg cggcgccgcc gcccttgatc aggcacttgt tggcgtcgca ctcatcggcg ccatcgacgt acagcgacag gttgccgctg tggttcagct cgatcacctc gatgccgtgc tgcttcaggc gcgcggtgct ctgctcggag ctggaaacag cgcccttgat gcggtgctgg atgcgggcca gggcatcgat gaaataggcc acggtggaac cggtgccgac accgacgatc atgccgtctt caacgtactc gatggctttc tcggcggcca ggcgcttggc ttcggacata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS EAKRLAAEKA IEYVEDGMIV GVGTGSTVAY FIDALARIQH RIKGAVSSSE QSTARLKQHG IEVIELNHSG NLSLYVDGAD ECDANKCLIK GGGAALTREK IIAEASERFI CIIDPSKQVP VLGRFPLPVE VIPMARSLVA RQIRDMTGGQ PTWREGVVTD NGNQILDIHN LQITDPEKLE RELNQLPGVV CVGLFARRRA DVVIVGGEPP VVL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG AAGCCAAGCG CCTGGCCGCC GAGAAAGCCA TCGAGTACGT TGAAGACGGC ATGATCGTCG GTGTCGGCAC CGGTTCCACC GTGGCCTATT TCATCGATGC CCTGGCCCGC ATCCAGCACC GCATCAAGGG CGCTGTTTCC AGCTCCGAGC AGAGCACCGC GCGCCTGAAG CAGCACGGCA TCGAGGTGAT CGAGCTGAAC CACAGCGGCA ACCTGTCGCT GTACGTCGAT GGCGCCGATG AGTGCGACGC CAACAAGTGC CTGATCAAGG GCGGCGGCGC CGCGCTGACC CGCGAGAAGA TCATCGCCGA GGCCAGCGAG CGCTTCATCT GCATCATCGA CCCGAGCAAG CAGGTGCCGG TGCTGGGCAG GTTCCCGCTG CCGGTGGAAG TGATCCCGAT GGCGCGCAGC CTGGTCGCCC GCCAGATCCG CGACATGACC GGCGGCCAGC CGACCTGGCG TGAAGGCGTG GTCACCGACA ACGGCAACCA GATCCTGGAC ATCCACAACC TGCAGATCAC CGATCCGGAA AAGCTGGAGC GCGAGCTCAA CCAGCTGCCG GGCGTGGTCT GCGTCGGCCT GTTCGCCCGC CGCCGCGCCG ATGTGGTGAT CGTCGGCGGC GAGCCGCCGG TCGTGCTCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT