StmaA.00940.a

Undecaprenyl diphosphate synthase (EC 2.5.1.31) (uppS)

CENTER ID: StmaA.00940.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00940.a.B1.GE43267 Full length(StmaA.00940.a) 1 248
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1439

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPATLPRHVA IIMDGNGRWA QQRRRPRVIG HRAGARAVNR TIERCLELGI PALTLFAFSS ENWGRPQEEV DALMKLFLGA LDREVDELHR RGVRVRFIGE RERFGAGLVS RMQLAEQRTA DNTTLTLSIA ASYGGRQDIA RAARALAAEV AAGRLLPEQI DESLLGSQVA LADLPPPDLF IRTGGDTRIS NFLLWQLAYT ELWFTEALWP DFDAALLQQA LDAYGSRERR FGLTSAQIAA LATETSSP
NT Sequence
TTGCCGGCCA CCCTGCCCCG CCACGTCGCC ATCATCATGG ATGGCAACGG GCGCTGGGCA CAGCAGCGCC GCCGCCCGCG CGTGATCGGC CATCGTGCCG GCGCGCGCGC GGTCAACCGC ACCATCGAGC GCTGCCTTGA ACTGGGCATT CCGGCGCTGA CCCTGTTCGC GTTTTCCAGC GAGAACTGGG GCCGGCCGCA GGAAGAAGTC GACGCCCTGA TGAAGCTGTT CCTCGGCGCG CTCGACCGCG AAGTGGACGA ACTGCACCGA CGCGGCGTGC GCGTACGCTT CATCGGCGAA CGCGAGCGCT TTGGTGCCGG ACTGGTCAGC CGCATGCAGC TGGCCGAGCA GCGCACCGCC GACAACACCA CCCTGACCCT GTCGATCGCC GCCAGCTACG GCGGTCGCCA GGACATCGCC CGTGCCGCGC GCGCACTGGC CGCTGAAGTC GCCGCAGGAC GCCTGCTGCC CGAACAGATC GACGAATCGC TGCTGGGCAG CCAGGTCGCC CTGGCCGACC TGCCGCCGCC GGATCTGTTC ATCCGCACCG GTGGCGACAC CCGCATCAGC AACTTCCTGC TGTGGCAACT GGCCTACACC GAGCTGTGGT TCACCGAGGC GCTGTGGCCG GACTTCGATG CCGCCCTGCT GCAGCAGGCG CTGGATGCCT ATGGCAGCCG CGAGCGTCGT TTCGGCCTGA CCAGCGCCCA GATCGCCGCA CTGGCCACCG AGACCTCCAG CCCA
Details for StmaA.00940.a.B1.GE43267
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 28kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 65
PERCENT COVERAGE: 31
Validated AA Sequence
MAHHHHHHMX VATLPRLLAV IMDGXGGWGL HHHRXDGIGN LAGARAVNRT IEGCLDLGIP ELIMFAVSXR TGAGRWRXST P
Validated NT Sequence
acgactcnct atagggagac cacaacggtt tccctctaga aataattttg tttaacttna agaaggngat ataccatggc tcaccaccac caccaccata tgncggtggc caccctgccc cgcctcctcg ccgtcatcat ggatgggnnc gggggctggg gcctgcacca ccaccgtncg gacgggatcg ggaatcttgc cggcgcgcgc gccgtcaacc gcaccatcga gggctgcctt gacctgggca ttccggagct gatcatgttc gcggtttcca nnagaactgg ggccgggcgc tggaggaant cgacgccctg atnaagctgt tcctcggngc gntcgacctc taagtgcaca aactgtaccg acnnngcgtg cgcgtacgct tgatcagcta acgcgaccgc tttgttgncg gactggtcag ccgcntgctg ntggccgagc acnacaccgc tgacnacgcc accctgaccc tgtcgatcgc cgntnnctac ngcggtcgcc angacatcgc cctggccggg ctggncctgg ccgctgaaga cgtcgcatga cgcctgctnn ccgangagat ccacnactcg ctgctggaca gccagatcgc cgtgntcgac ctgnnntgcc ggatctgttc tccatctcgg cgatcgactt ccanataatg atcgttcctg ctggnagnac tggcctacac cgagctctgg gtcgccgnng ctntgnggcc ggacttcgag gccgncctgg ctgcgnnngn gctgnatgcc ctatgcctnc nagancnnnt cncactgncn nnnngnnnat cgcngcactg ncccccacac cngnnncccn nagngatnaa cnaangatnn ngcnnntnaa anaaccnnaa ggnanctnna gnngggggct tccnacnnnn gatcatgncc tatnttaanc cccntggggc cncnaanccg ggacttgnng gggttnttng ctggaaagnn nnnnnnnnnn nncgggatat cccccggnac cggcnggggn nnnccatgat tgnnnnnatc ncaannnggg ntcccannnn ccgaagcnan nggggcggnn ggccnanntc gtncnnnnac tgnttnngnn nnacnnggga cnnnnannnn aaattngnnt naannnncnt annngnnnna nnnnnncncn annna
Expected Protein Sequence
MAHHHHHHMP ATLPRHVAII MDGNGRWAQQ RRRPRVIGHR AGARAVNRTI ERCLELGIPA LTLFAFSSEN WGRPQEEVDA LMKLFLGALD REVDELHRRG VRVRFIGERE RFGAGLVSRM QLAEQRTADN TTLTLSIAAS YGGRQDIARA ARALAAEVAA GRLLPEQIDE SLLGSQVALA DLPPPDLFIR TGGDTRISNF LLWQLAYTEL WFTEALWPDF DAALLQQALD AYGSRERRFG LTSAQIAALA TETSSP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgccgg ccaccctgcc ccgccacgtc gccatcatca tggatggcaa cgggcgctgg gcacagcagc gccgccgccc gcgcgtgatc ggccatcgtg ccggcgcgcg cgcggtcaac cgcaccatcg agcgctgcct tgaactgggc attccggcgc tgaccctgtt cgcgttttcc agcgagaact ggggccggcc gcaggaagaa gtcgacgccc tgatgaagct gttcctcggc gcgctcgacc gcgaagtgga cgaactgcac cgacgcggcg tgcgcgtacg cttcatcggc gaacgcgagc gctttggtgc cggactggtc agccgcatgc agctggccga gcagcgcacc gccgacaaca ccaccctgac cctgtcgatc gccgccagct acggcggtcg ccaggacatc gcccgtgccg cgcgcgcact ggccgctgaa gtcgccgcag gacgcctgct gcccgaacag atcgacgaat cgctgctggg cagccaggtc gccctggccg acctgccgcc gccggatctg ttcatccgca ccggtggcga cacccgcatc agcaacttcc tgctgtggca actggcctac accgagctgt ggttcaccga ggcgctgtgg ccggacttcg atgccgccct gctgcagcag gcgctggatg cctatggcag ccgcgagcgt cgtttcggcc tgaccagcgc ccagatcgcc gcactggcca ccgagacctc cagcccatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at