StmaA.00888.a

Adenylosuccinate synthetase (EC 6.3.4.4) (purA)

CENTER ID: StmaA.00888.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00888.a.B1.GE43266 Full length(StmaA.00888.a) 1 430
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3372

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGQSVVVLGA QWGDEGKGKI VDLLTEEIGA VVRFQGGHNA GHTLVINGKK TVLHLIPSGI LRDDALCLIG NGVVISPAAL QKEIAELEAS GVEVRSRLKI SPAAPLIMPY HIALDQARER AAGGKAIGTT GRGIGPAYED KVARRGIRIA DLHYPKQLEE LLRTALDYHN FVLTKYLNTD AVDFQKTFDE ALAFGEYVQP MKSDVAGILH DLRKQGKRVL FEGAQGALLD IDHGTYPYVT SSNTTVGGAL AGSGVGADSI DYVLGIAKAY ATRVGGGPFP TELDDEVGQG IRDRGAEYGA STGRPRRCGW MDIVALKRAV AINGISGLCI TKLDVLDGME KLKVCIAYEY NGKRTEYAPL DAQGWEECTP VYLEFPGWSE NTHGITNWDD LPPAARAYLR SLEELAGCPI SIVSTGPDRD HTMVLQDPFA
NT Sequence
ATGGGTCAGT CTGTCGTAGT GTTGGGTGCC CAGTGGGGCG ATGAAGGCAA GGGCAAGATC GTCGATCTGC TCACTGAGGA AATCGGCGCC GTCGTGCGCT TCCAGGGCGG CCACAACGCC GGCCACACCC TGGTCATCAA TGGCAAGAAG ACCGTCCTGC ACCTGATCCC GTCGGGCATC CTGCGTGACG ACGCGCTGTG CCTGATCGGC AACGGCGTGG TGATCTCGCC GGCCGCGCTG CAGAAGGAAA TCGCCGAGCT GGAAGCCTCC GGCGTGGAAG TGCGTTCGCG CCTGAAGATC TCCCCGGCCG CGCCGCTGAT CATGCCGTAC CACATCGCCC TGGACCAGGC GCGCGAGCGC GCTGCCGGTG GCAAGGCGAT CGGCACCACC GGCCGTGGCA TCGGCCCGGC CTACGAAGAC AAGGTGGCGC GTCGCGGCAT CCGCATCGCC GACCTGCATT ATCCGAAGCA GCTGGAAGAG CTCCTGCGCA CTGCGCTGGA CTACCACAAC TTCGTGCTGA CCAAGTATCT GAACACCGAT GCGGTCGATT TCCAGAAGAC CTTCGACGAA GCGCTGGCCT TCGGCGAATA CGTGCAGCCG ATGAAGTCCG ACGTGGCCGG CATCCTGCAC GACCTGCGCA AGCAGGGTAA GCGCGTGCTG TTTGAAGGTG CGCAGGGTGC GCTGCTGGAT ATCGACCACG GCACCTACCC GTACGTCACC AGCTCCAACA CCACCGTCGG TGGCGCGCTG GCCGGTTCCG GCGTCGGCGC CGATTCGATC GACTACGTGC TGGGCATCGC CAAGGCCTAC GCCACCCGCG TCGGCGGTGG CCCATTCCCG ACCGAGCTGG ACGACGAAGT CGGCCAGGGC ATCCGTGACC GCGGCGCCGA GTACGGTGCG TCGACCGGCC GTCCGCGTCG TTGCGGCTGG ATGGACATCG TCGCGCTGAA GCGTGCCGTG GCCATCAACG GCATCAGCGG CCTGTGCATC ACCAAGCTGG ACGTGCTGGA CGGCATGGAA AAGCTGAAGG TCTGCATCGC CTACGAATAC AACGGCAAGC GCACCGAGTA CGCGCCGCTG GACGCGCAGG GCTGGGAAGA GTGCACGCCG GTGTACCTGG AGTTCCCGGG CTGGAGCGAG AACACCCACG GCATCACCAA CTGGGACGAC CTGCCGCCGG CTGCGCGCGC CTACCTGCGT TCGCTGGAAG AGCTGGCCGG CTGCCCGATC AGCATCGTCT CCACCGGTCC GGACCGCGAC CACACCATGG TCCTGCAGGA TCCGTTCGCC
Details for StmaA.00888.a.B1.GE43266
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 47kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG QSVVVLGAQW GDEGKGKIVD LLTEEIGAVV RFQGGHNAGH TLVINGKKTV LHLIPSGILR DDALCLIGNG VVISPAALQK EIAELEASGV EVRSRLKISP AAPLIMPYHI ALDQARERAA GGKAIGTTGR GIGPAYEDKV ARRGIRIADL HYPKQLEELL RTALDYHNFV LTKYLNTDAV DFQKTFDEAL AFGEYVQPMK SDVAGILHDL RKQGKRVLFE GAQGALLDID HGTYPYVTSS NTTVGGALAG SGVGADSIDY VLGIAKAYAT RVGGGPFPTE LDDEVGQGIR DRGAEYGAST GRPRRCGWMD IVALKRAVAI NGISGLCITK LDVLDGMEKL KVCIAYEYNG KRTEYAPLDA QGWEECTPVY LEFPGWSENT HGITNWDDLP PAARAYLRSL EELAGCPISI VSTGPDRDHT MVLQDPFA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc agtctgtcgt agtgttgggt gcccagtggg gcgatgaagg caagggcaag atcgtcgatc tgctcactga ggaaatcggc gccgtcgtgc gcttccaggg cggccacaac gccggccaca ccctggtcat caatggcaag aagaccgtcc tgcacctgat cccgtcgggc atcctgcgtg acgacgcgct gtgcctgatc ggcaacggcg tggtgatctc gccggccgcg ctgcagaagg aaatcgccga gctggaagcc tccggcgtgg aagtgcgttc gcgcctgaag atctccccgg ccgcgccgct gatcatgccg taccacatcg ccctggacca ggcgcgcgag cgcgctgccg gtggcaaggc gatcggcacc accggccgtg gcatcggccc ggcctacgaa gacaaggtgg cgcgtcgcgg catccgcatc gccgacctgc attatccgaa gcagctggaa gagctcctgc gcactgcgct ggactaccac aacttcgtgc tgaccaagta tctgaacacc gatgcggtcg atttccagaa gaccttcgac gaagcgctgg ccttcggcga atacgtgcag ccgatgaagt ccgacgtggc cggcatcctg cacgacctgc gcaagcaggg taagcgcgtg ctgtttgaag gtgcgcaggg tgcgctgctg gatatcgacc acggcaccta cccgtacgtc accagctcca acaccaccgt cggtggcgcg ctggccggtt ccggcgtcgg cgccgattcg atcgactacg tgctgggcat cgccaaggcc tacgccaccc gcgtcggcgg tggcccattc ccgaccgagc tggacgacga agtcggccag ggcatccgtg accgcggcgc cgagtacggt gcgtcgaccg gccgtccgcg tcgttgcggc tggatggaca tcgtcgcgct gaagcgtgcc gtggccatca acggcatcag cggcctgtgc atcaccaagc tggacgtgct ggacggcatg gaaaagctga aggtctgcat cgcctacgaa tacaacggca agcgcaccga gtacgcgccg ctggacgcgc agggctggga agagtgcacg ccggtgtacc tggagttccc gggctggagc gagaacaccc acggcatcac caactgggac gacctgccgc cggctgcgcg cgcctacctg cgttcgctgg aagagctggc cggctgcccg atcagcatcg tctccaccgg tccggaccgc gaccacacca tggtcctgca ggatccgttc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat