StmaA.00856.a

Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1) (hemH)

CENTER ID: StmaA.00856.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00856.a.B1.GE43265 Full length(StmaA.00856.a) 1 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.4377

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLAVNLGTPE TPTAPAVRRY LAEFLSDRRV VAIPPLLWQP LLRGLILPLR SSRSAAKYAQ VWLPDGSPLM VHTRQLAQAM QALLPTLTVR HAMRYGEPAL ASELDRLAAD GARRIVVLPL YPQYSTTTTA SVEDRVDAWQ RRNPGVTVSL VRDYSVDPGW VEAVAGSIRR YWEQHGRGQM LMFSFHGIPQ RLADAGDPYP QRCEASAQAI AKALELGRDE WQMGYQSRFG RERWLQPYAE PSLWALAESG VKQIDVVCPG FATDCLETLE EVALGFTETL AERGATMRYI PCLNAEPEHA RALARLAVAS LA
NT Sequence
GTGCTGGCGG TCAACCTAGG CACGCCCGAG ACGCCGACGG CCCCCGCCGT ACGCCGTTAC CTGGCTGAAT TCCTGTCCGA CCGCCGCGTG GTCGCGATCC CGCCACTGCT GTGGCAGCCG CTGCTGCGCG GACTGATCCT GCCCCTGCGC AGCAGTCGTT CGGCGGCCAA GTACGCCCAG GTCTGGCTGC CCGACGGCTC ACCGCTGATG GTGCATACGC GCCAGCTGGC GCAGGCCATG CAGGCCCTGT TGCCGACCCT GACAGTGCGT CATGCGATGC GTTACGGCGA ACCCGCGCTG GCCAGCGAGC TCGACCGCCT GGCAGCCGAC GGTGCGCGCC GCATCGTGGT GCTGCCGCTG TATCCGCAGT ACTCCACCAC CACCACTGCC TCGGTCGAAG ACCGCGTCGA TGCCTGGCAG CGCCGCAATC CTGGCGTGAC CGTCAGCCTG GTGCGCGACT ATTCGGTCGA TCCGGGCTGG GTCGAGGCCG TGGCCGGCTC GATCCGTCGC TACTGGGAAC AGCACGGTCG CGGCCAGATG CTGATGTTCT CCTTCCATGG CATCCCGCAG CGCCTGGCCG ATGCCGGCGA TCCGTATCCG CAGCGCTGCG AGGCCAGCGC ACAGGCCATC GCCAAGGCGC TGGAACTGGG CAGGGACGAG TGGCAGATGG GTTACCAGTC GCGTTTCGGC CGCGAGCGCT GGCTGCAGCC GTATGCCGAG CCCAGCCTGT GGGCGCTGGC CGAATCCGGC GTGAAGCAGA TCGACGTGGT GTGCCCCGGC TTCGCCACCG ACTGCCTGGA GACGCTGGAA GAAGTCGCGC TAGGGTTCAC CGAAACACTG GCCGAGCGCG GTGCGACGAT GCGCTACATC CCCTGCCTCA ACGCCGAACC GGAGCACGCA CGCGCGCTTG CGCGGCTGGC CGTGGCCTCG CTGGCA
Details for StmaA.00856.a.B1.GE43265
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 55
Validated AA Sequence
MAHHHHHHMV LAVNLGTPET PTAPAVRRYL AEFLSDRRVV AIPPLLWQPL LRGLILPLRS SHSAAKYAQV WLPDGSPLMV HTRQLAQAMQ ALLPTLTVRH AMRYGEPALA SELDRLAADG ARRIVVLPLY PQYSTTTTAS VEDRVDAWQR RNPGVTVSLV RDYSVDPGWV EAEASXV
Validated NT Sequence
acgactcnct atagggagac cncaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcaccaccac caccaccata tggtgctggc ggtcaaccta ggcacgcccg agacgccgac ggcccccgcc gtacgccgtt acctggctga attcctgtcc gaccgccgcg tggtcgcgat cccgccactg ctgtggcagc cgctgctgcg cggactgatc ctgcccctgc gcagcagtca ttcggcggcc aagtacgccc aggtctggct gcccgacggc tcaccgctga tggtgcatac gcgccagctg gcgcaggcca tgcaggccct gttgccgacc ctgacagtgc gtcatgcgat gcgttacggc gaacccgcgc tggccagcga gctcgaccgc ctggcagccg acggtgcgcg ccgcatcgtg gtgctgccgc tgtatccgca gtactccacc accaccactg cctcggtcga agaccgcgtc gatgcctggc agcgccgcaa tcctggcgtg accgtcagcc tggtgcgcga ctattcggtc gatccgggct gggtcgaggc cgaggcctcn ncggtataac tgtnctggan angatccggc tgctancana tgccgangtg aanctgacnn nnnnnctgcc accgctnagc nnanactann atanccccnt tggnancgct nnnnggctct tganngggna tcgnnnannn gatgnnntnt gtccngatat antggggaca ggggtggant catgattcnn nngccnntnn ngnntncaan tanntaacna gcaagactgt gngnggtgaa canatgnngg atgagctcat agancgggtg ngccnanntt tgctcnnnnt nnntnggnnn nnnctgcann nnnttactgn ctggntatac tgaannnatg gcnnancntn nnngnnnnnn ncccgnnnnt nccnctgnta nncnanncnn anncgnanca ncgtccnnng nnnnngnggn ctaggcngan gnatnnncnn gntttnnnnn attaatannn nnnnccgnnn ntgtaannan cnnnnnnnan nntnaantan cngcntnnnn nnnntnnnnn ananaannnn t
Expected Protein Sequence
MAHHHHHHML AVNLGTPETP TAPAVRRYLA EFLSDRRVVA IPPLLWQPLL RGLILPLRSS RSAAKYAQVW LPDGSPLMVH TRQLAQAMQA LLPTLTVRHA MRYGEPALAS ELDRLAADGA RRIVVLPLYP QYSTTTTASV EDRVDAWQRR NPGVTVSLVR DYSVDPGWVE AVAGSIRRYW EQHGRGQMLM FSFHGIPQRL ADAGDPYPQR CEASAQAIAK ALELGRDEWQ MGYQSRFGRE RWLQPYAEPS LWALAESGVK QIDVVCPGFA TDCLETLEEV ALGFTETLAE RGATMRYIPC LNAEPEHARA LARLAVASLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgctgg cggtcaacct aggcacgccc gagacgccga cggcccccgc cgtacgccgt tacctggctg aattcctgtc cgaccgccgc gtggtcgcga tcccgccact gctgtggcag ccgctgctgc gcggactgat cctgcccctg cgcagcagtc gttcggcggc caagtacgcc caggtctggc tgcccgacgg ctcaccgctg atggtgcata cgcgccagct ggcgcaggcc atgcaggccc tgttgccgac cctgacagtg cgtcatgcga tgcgttacgg cgaacccgcg ctggccagcg agctcgaccg cctggcagcc gacggtgcgc gccgcatcgt ggtgctgccg ctgtatccgc agtactccac caccaccact gcctcggtcg aagaccgcgt cgatgcctgg cagcgccgca atcctggcgt gaccgtcagc ctggtgcgcg actattcggt cgatccgggc tgggtcgagg ccgtggccgg ctcgatccgt cgctactggg aacagcacgg tcgcggccag atgctgatgt tctccttcca tggcatcccg cagcgcctgg ccgatgccgg cgatccgtat ccgcagcgct gcgaggccag cgcacaggcc atcgccaagg cgctggaact gggcagggac gagtggcaga tgggttacca gtcgcgtttc ggccgcgagc gctggctgca gccgtatgcc gagcccagcc tgtgggcgct ggccgaatcc ggcgtgaagc agatcgacgt ggtgtgcccc ggcttcgcca ccgactgcct ggagacgctg gaagaagtcg cgctagggtt caccgaaaca ctggccgagc gcggtgcgac gatgcgctac atcccctgcc tcaacgccga accggagcac gcacgcgcgc ttgcgcggct ggccgtggcc tcgctggcat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat