StmaA.00852.a

GTP-binding protein EngA (engA)

CENTER ID: StmaA.00852.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00852.a.B1.GE43264 Full length(StmaA.00852.a) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1963

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLPLVALVGR PNVGKSTIFN ALTRTRDALV HDQPGVTRDR NYGVCRLDED NHFLVVDTGG IAGEDEGLAG ATTRQARAAA AEADLILFVV DAREGTSALD DEILAWLRKL SRPTLLLINK IDGTDEDSVR SEFARYGFGE MLTVSAAHRQ GLDDLLDEVI QRLPEEGSGE ELDNDPNRIR IAFVGRPNVG KSTLVNRILG EERMIASDVP GTTRDSIAVD LERDGREYRL IDTAGLRRRS RVDEVVEKFS VVKTMQSIEQ CQVAVLMLDA TEGVTDQDAT VLGAVLDAGR ALVIAINKWD GLTEYQREQA ETMLSLKLGF VPWAESVRIS AKHGSGLREL FRAVHRAHES ANKTFTTSEV NKALEVAYET NPPPTIRGHV SKLRYVHPAG ANPPTFIVHG TRLKELQESY KRYLENFFRK RFKLIGTPVS FIFREGTNPY EGKKNVLTER QVKAKRRLMK HVKGK
NT Sequence
ATGCTGCCTT TGGTCGCCCT GGTTGGACGG CCGAATGTCG GCAAGTCGAC TATTTTCAAT GCGCTTACGC GCACCCGCGA CGCGCTGGTC CATGACCAGC CCGGCGTCAC CCGTGACCGC AACTACGGCG TCTGCCGCCT CGACGAGGAC AACCATTTCC TCGTCGTGGA TACCGGCGGT ATTGCCGGGG AAGACGAAGG CCTGGCTGGT GCCACCACGC GCCAGGCCCG GGCTGCGGCG GCCGAAGCCG ATCTGATCCT GTTCGTCGTC GACGCCCGCG AGGGCACCTC GGCGCTGGAC GACGAGATCC TGGCCTGGCT GCGCAAGCTG TCGCGCCCGA CGCTGCTGCT GATCAACAAG ATCGACGGCA CCGACGAGGA CAGCGTGCGT TCGGAATTCG CCCGTTACGG CTTCGGCGAG ATGCTGACCG TTTCGGCCGC GCATCGCCAA GGCCTGGACG ACCTGCTGGA CGAAGTGATC CAGCGCCTGC CGGAAGAAGG CAGCGGAGAA GAGCTGGACA ACGATCCGAA CCGTATCCGC ATCGCCTTCG TCGGACGTCC GAACGTGGGC AAGTCGACCC TGGTCAACCG CATCCTCGGC GAGGAGCGCA TGATCGCCTC CGACGTGCCG GGCACCACCC GCGACTCGAT CGCGGTGGAC CTGGAGCGCG ACGGCCGTGA GTACCGCCTG ATCGACACCG CCGGCCTGCG CCGCCGTTCG CGCGTGGACG AGGTCGTCGA GAAGTTCTCG GTGGTCAAGA CCATGCAGTC GATCGAGCAG TGCCAGGTCG CGGTGCTGAT GCTGGACGCC ACCGAAGGCG TGACCGACCA GGACGCCACC GTCCTCGGTG CCGTGCTCGA CGCCGGCCGC GCGCTGGTGA TCGCCATCAA CAAGTGGGAT GGCCTGACCG AGTACCAGCG CGAACAGGCT GAAACCATGC TGTCGCTGAA GCTGGGCTTC GTGCCGTGGG CCGAATCGGT GCGCATTTCG GCCAAGCACG GCTCGGGCCT GCGCGAGCTG TTCCGTGCGG TGCACCGCGC GCACGAATCG GCGAACAAGA CCTTCACCAC CAGCGAAGTG AACAAGGCGC TGGAAGTGGC CTACGAGACC AACCCGCCGC CGACCATCCG CGGCCACGTC TCCAAGCTGC GCTACGTGCA CCCGGCCGGC GCAAACCCGC CGACCTTCAT CGTGCACGGC ACCCGCCTGA AGGAACTGCA GGAGTCGTAC AAGCGCTATC TGGAGAACTT CTTCCGCAAG CGCTTCAAGC TGATCGGCAC GCCGGTGAGC TTCATCTTCC GCGAGGGCAC CAACCCGTAC GAGGGCAAGA AGAACGTCCT CACCGAGCGG CAGGTCAAGG CCAAGCGGCG CTTGATGAAG CACGTCAAGG GCAAG
Details for StmaA.00852.a.B1.GE43264
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 53kDa
OBSERVED MW: 18kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 91
PERCENT COVERAGE: 44
Validated AA Sequence
MAHHHHHHML PLVALVGRPN VGKSTIFNAL TRTRDALVHD QPGVTRDRNY GVCRLDEDNH FLVVDTGGIA GEDEGLAGAT TRQARAAAAE ADLILFVVDA REGTSALDDE ILAWLLKLSR PTLLLINKID GTDEDSXCSE FARYXFGEML TVSAAHXQGL DDLLDXVIQR LPEEXSGEEL DNDPNRIRIS FFGRPNVGKS TLGQPHPRRG SA
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML PLVALVGRPN VGKSTIFNAL TRTRDALVHD QPGVTRDRNY GVCRLDEDNH FLVVDTGGIA GEDEGLAGAT TRQARAAAAE ADLILFVVDA REGTSALDDE ILAWLRKLSR PTLLLINKID GTDEDSVRSE FARYGFGEML TVSAAHRQGL DDLLDEVIQR LPEEGSGEEL DNDPNRIRIA FVGRPNVGKS TLVNRILGEE RMIASDVPGT TRDSIAVDLE RDGREYRLID TAGLRRRSRV DEVVEKFSVV KTMQSIEQCQ VAVLMLDATE GVTDQDATVL GAVLDAGRAL VIAINKWDGL TEYQREQAET MLSLKLGFVP WAESVRISAK HGSGLRELFR AVHRAHESAN KTFTTSEVNK ALEVAYETNP PPTIRGHVSK LRYVHPAGAN PPTFIVHGTR LKELQESYKR YLENFFRKRF KLIGTPVSFI FREGTNPYEG KKNVLTERQV KAKRRLMKHV KGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc ctttggtcgc cctggttgga cggccgaatg tcggcaagtc gactattttc aatgcgctta cgcgcacccg cgacgcgctg gtccatgacc agcccggcgt cacccgtgac cgcaactacg gcgtctgccg cctcgacgag gacaaccatt tcctcgtcgt ggataccggc ggtattgccg gggaagacga aggcctggct ggtgccacca cgcgccaggc ccgggctgcg gcggccgaag ccgatctgat cctgttcgtc gtcgacgccc gcgagggcac ctcggcgctg gacgacgaga tcctggcctg gctgcgcaag ctgtcgcgcc cgacgctgct gctgatcaac aagatcgacg gcaccgacga ggacagcgtg cgttcggaat tcgcccgtta cggcttcggc gagatgctga ccgtttcggc cgcgcatcgc caaggcctgg acgacctgct ggacgaagtg atccagcgcc tgccggaaga aggcagcgga gaagagctgg acaacgatcc gaaccgtatc cgcatcgcct tcgtcggacg tccgaacgtg ggcaagtcga ccctggtcaa ccgcatcctc ggcgaggagc gcatgatcgc ctccgacgtg ccgggcacca cccgcgactc gatcgcggtg gacctggagc gcgacggccg tgagtaccgc ctgatcgaca ccgccggcct gcgccgccgt tcgcgcgtgg acgaggtcgt cgagaagttc tcggtggtca agaccatgca gtcgatcgag cagtgccagg tcgcggtgct gatgctggac gccaccgaag gcgtgaccga ccaggacgcc accgtcctcg gtgccgtgct cgacgccggc cgcgcgctgg tgatcgccat caacaagtgg gatggcctga ccgagtacca gcgcgaacag gctgaaacca tgctgtcgct gaagctgggc ttcgtgccgt gggccgaatc ggtgcgcatt tcggccaagc acggctcggg cctgcgcgag ctgttccgtg cggtgcaccg cgcgcacgaa tcggcgaaca agaccttcac caccagcgaa gtgaacaagg cgctggaagt ggcctacgag accaacccgc cgccgaccat ccgcggccac gtctccaagc tgcgctacgt gcacccggcc ggcgcaaacc cgccgacctt catcgtgcac ggcacccgcc tgaaggaact gcaggagtcg tacaagcgct atctggagaa cttcttccgc aagcgcttca agctgatcgg cacgccggtg agcttcatct tccgcgaggg caccaacccg tacgagggca agaagaacgt cctcaccgag cggcaggtca aggccaagcg gcgcttgatg aagcacgtca agggcaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat