StmaA.00725.a

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148) (ipk)

CENTER ID:	StmaA.00725.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00725.a.B1.GE43261	Full length(StmaA.00725.a)		1	295
StmaA.00725.a.B1.GE44005	Full length(StmaA.00725.a)		1	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|522373.3.peg.846

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGAGHPMSAG RDDTGWSWWP APAKLNLFLH ITGRRADGYH ELQTVFRLLD WGDRIGLHLR EDGQVRRQGE GLAGVAEADD LAVRAARLLK DAANIVQGAD IIVEKHIPAG GGFGGGSSDA ATVLVVLNRL WRAGLDEDAL AALGLRLGAD VPVFVRGRNA WAEGVGERLQ PIVLAPAWYV VVEPGVHVPT PALFADPDLT RGSPVAKIED FASGTLVGNA FEPVLRRREP AVEAAFAALS DIGTARLTGS GSGCFVEFAS QSAAEQGRSK LPKELRARVA AGVARSPLLD ALEQH

NT Sequence

ATGGGGGCAG GGCACCCCAT GAGTGCCGGG CGCGATGACA CGGGCTGGTC CTGGTGGCCG GCCCCGGCCA AGCTGAACCT GTTCCTGCAC ATCACCGGCC GCCGTGCCGA TGGCTACCAC GAGCTGCAGA CCGTGTTCCG CCTGCTGGAC TGGGGCGACC GCATCGGTCT GCACCTGCGT GAAGACGGCC AGGTGCGCCG GCAGGGCGAG GGGCTGGCTG GCGTGGCCGA GGCGGATGAC CTGGCGGTCC GCGCCGCGCG GCTTCTGAAA GATGCGGCCA ATATCGTGCA AGGCGCGGAC ATCATCGTCG AAAAACACAT TCCTGCAGGT GGTGGCTTTG GCGGCGGTTC GTCCGACGCG GCCACCGTAC TGGTGGTGCT GAACCGGCTC TGGCGTGCCG GTCTGGACGA GGACGCGCTT GCTGCGCTGG GCCTGCGCCT GGGCGCCGAC GTGCCGGTGT TCGTGCGCGG GCGCAATGCC TGGGCCGAAG GGGTGGGTGA GCGCCTGCAG CCGATCGTGC TGGCGCCGGC CTGGTATGTG GTGGTTGAAC CGGGTGTTCA TGTTCCCACC CCGGCTCTGT TCGCAGACCC GGATTTGACG CGCGGCAGCC CAGTGGCGAA AATAGAGGAC TTCGCTTCCG GGACCCTGGT CGGGAACGCG TTCGAACCGG TGCTGCGCCG CCGTGAGCCT GCCGTCGAGG CAGCGTTCGC CGCGTTGAGC GACATCGGCA CGGCGCGGCT GACCGGTTCG GGAAGTGGTT GTTTCGTCGA GTTCGCATCG CAGTCTGCCG CAGAGCAGGG ACGGTCGAAG TTGCCGAAGG AGTTGCGGGC AAGGGTGGCA GCGGGCGTTG CGCGTTCGCC ACTGCTGGAT GCACTCGAGC AACAC

Details for StmaA.00725.a.B1.GE43261

HARVESTED ON:	data unavailable
SEQUENCED ON:	6/18/2019
EXPECTED MW:	32kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	13

Validated AA Sequence

MAHHHHHHMG AGXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXRSKLP KELRARVAAG VARSPLLDAL EQH

Validated NT Sequence

atggctcacc accaccacca ccatatg

Expected Protein Sequence

MAHHHHHHMG AGHPMSAGRD DTGWSWWPAP AKLNLFLHIT GRRADGYHEL QTVFRLLDWG DRIGLHLRED GQVRRQGEGL AGVAEADDLA VRAARLLKDA ANIVQGADII VEKHIPAGGG FGGGSSDAAT VLVVLNRLWR AGLDEDALAA LGLRLGADVP VFVRGRNAWA EGVGERLQPI VLAPAWYVVV EPGVHVPTPA LFADPDLTRG SPVAKIEDFA SGTLVGNAFE PVLRRREPAV EAAFAALSDI GTARLTGSGS GCFVEFASQS AAEQGRSKLP KELRARVAAG VARSPLLDAL EQH

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggggg cagggcaccc catgagtgcc gggcgcgatg acacgggctg gtcctggtgg ccggccccgg ccaagctgaa cctgttcctg cacatcaccg gccgccgtgc cgatggctac cacgagctgc agaccgtgtt ccgcctgctg gactggggcg accgcatcgg tctgcacctg cgtgaagacg gccaggtgcg ccggcagggc gaggggctgg ctggcgtggc cgaggcggat gacctggcgg tccgcgccgc gcggcttctg aaagatgcgg ccaatatcgt gcaaggcgcg gacatcatcg tcgaaaaaca cattcctgca ggtggtggct ttggcggcgg ttcgtccgac gcggccaccg tactggtggt gctgaaccgg ctctggcgtg ccggtctgga cgaggacgcg cttgctgcgc tgggcctgcg cctgggcgcc gacgtgccgg tgttcgtgcg cgggcgcaat gcctgggccg aaggggtggg tgagcgcctg cagccgatcg tgctggcgcc ggcctggtat gtggtggttg aaccgggtgt tcatgttccc accccggctc tgttcgcaga cccggatttg acgcgcggca gcccagtggc gaaaatagag gacttcgctt ccgggaccct ggtcgggaac gcgttcgaac cggtgctgcg ccgccgtgag cctgccgtcg aggcagcgtt cgccgcgttg agcgacatcg gcacggcgcg gctgaccggt tcgggaagtg gttgtttcgt cgagttcgca tcgcagtctg ccgcagagca gggacggtcg aagttgccga aggagttgcg ggcaagggtg gcagcgggcg ttgcgcgttc gccactgctg gatgcactcg agcaacactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for StmaA.00725.a.B1.GE44005

HARVESTED ON:	data unavailable
SEQUENCED ON:	12/16/2020
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	13

Validated AA Sequence

Validated NT Sequence

atggctcacc accaccacca ccatatg

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)