StmaA.00690.a

Efflux ABC transporter, ATP-binding protein

CENTER ID: StmaA.00690.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00690.a.B1.GE43260 Full length(StmaA.00690.a) 1 298
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.342

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDPIAPSLAR LQQVQVRYRD HTALQGIDLQ VRAGQVLALL GRNGAGKSTA ISVLLGLRRA DAGQVELLGG DPQQRSSRLG LGVMLQSTSL PPMLQVDELV AQASACYPDP MPLQEVLQRA GLQALAGRRY GQLSGGQQRA VQFAIALCGR PRVLFLDEPT TGLDIQARQA MWQAIRQLVA EGCGVLLTTH YLEEAEALAQ QVVVLEQGRV LADAPLSELR LADRPRRIRC RSVLTVEDVQ QWPGVQQVQR DGEHLQLMAS PAEPVVARLL AADAQLRELE VQGAALVDAF LDMTREAA
NT Sequence
ATGGATCCGA TCGCCCCGTC GCTGGCCCGC CTGCAGCAGG TGCAGGTGCG CTACCGCGAC CATACCGCCC TGCAGGGCAT CGACCTGCAG GTCCGCGCTG GCCAGGTGCT GGCATTGCTG GGCCGCAACG GTGCCGGCAA GAGTACCGCG ATCAGCGTGC TGTTGGGCCT GCGCCGCGCC GATGCCGGCC AGGTCGAGCT GCTCGGTGGC GACCCGCAGC AGCGCAGCAG CCGCCTCGGC CTGGGCGTGA TGCTGCAGAG CACCAGCCTG CCGCCGATGC TGCAGGTGGA CGAACTGGTG GCGCAGGCCA GCGCCTGCTA TCCGGACCCG ATGCCGCTGC AGGAGGTGCT GCAGCGCGCA GGCCTGCAGG CACTGGCAGG CCGTCGCTAC GGTCAGCTGT CCGGTGGCCA GCAGCGCGCG GTGCAGTTCG CCATCGCGCT GTGCGGCCGC CCGCGCGTGC TGTTCCTGGA CGAGCCCACC ACCGGCCTGG ACATCCAGGC GCGGCAGGCG ATGTGGCAGG CGATCCGGCA GCTGGTGGCC GAAGGCTGCG GCGTGCTGCT GACCACCCAC TATCTGGAAG AGGCCGAAGC GCTGGCGCAG CAGGTGGTGG TGCTGGAGCA GGGCCGGGTG CTGGCCGACG CACCGTTGAG CGAGCTGCGG CTGGCCGATC GGCCGCGCCG CATCCGCTGC CGCAGCGTGT TGACTGTTGA AGACGTACAG CAGTGGCCGG GCGTGCAGCA GGTGCAGCGT GATGGCGAGC ACCTGCAGTT GATGGCAAGC CCGGCCGAGC CGGTGGTGGC GCGCCTGCTG GCCGCCGATG CACAGCTGCG TGAGCTGGAA GTACAGGGCG CGGCACTGGT CGACGCTTTC CTCGACATGA CCCGGGAGGC CGCA
Details for StmaA.00690.a.B1.GE43260
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 54
PERCENT COVERAGE: 34
Validated AA Sequence
MAHHHHHHMX XKXPSXVXNA XXEVCYRDHX AXHGIDLXVR AGQAQALLVR KDAGMSTAIW VLLGLPQADD GQVQLLGGFP XXRXMXDXXV XMYLSSTLXL MLXXXNW
Validated NT Sequence
annnnnnnnn tnnnactcnc tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttn angaaggaga tataccatgg ctcaccacca ccaccaccat atggntnana aanncccgag cntggtcncc aatgcantgg nggaggtgtg ctaccgcgac catancgccc ngcatggcat cgacctggan gtccgcgctg gncaggcgca ggccttgctg gtgcgcaagg acgccggcat gagtaccgcc atctgggtgc tgttgggtct gccccaggcc gatgacgggc aggtccagct gctcggtggc ttcccnangn accgcncnat ganggacngn ntggtcncga tgtacctcag ctccaccctg anactgatgc tgnccngaaa naactggtga cncangncag cgnctgctat ccggacccga tgccgntggn ggangtgctg aagtnggcng gcctgnaagt ggtggcanga aagcgttacn gtcanctgtc cggtggncag ancncgcngg ntacgatcag tcnanctgng cnnccgaccg cgcgtgnggn ncctgcagga gcccannncg gcctgnacna cataactgcg cccgcnatgt ggnngcnatc cgatctctgg ngactgaatg ctgcagcatg ctgctaaacc acccactagn ngaaagangc cnaaaacgct ggcgcaanct gggtgngcga tagctnaggn atggntagct ngnctaccca ccgcccanca anntgnngtt gaactgtntt tcgcgtcctg nngntnncgt ncgcnatatg nntagnaaan angtcnnnac ctcgnccann gtcnanaant anntnnanat cagaacnann anatctgtga nganctccac anannnnntg ancnggctnc gtccngcngg gnngcantna nnactgganc naganccagn cannnactng ngtaccccac tatnnaatgn cnnannnatg tgaccganag gncccacccg nagnnnnaac acgganccgn nnaatcnnnn nnaccncnan nnnnnnnnnn nngntgggcn ccacnncnnc nnng
Expected Protein Sequence
MAHHHHHHMD PIAPSLARLQ QVQVRYRDHT ALQGIDLQVR AGQVLALLGR NGAGKSTAIS VLLGLRRADA GQVELLGGDP QQRSSRLGLG VMLQSTSLPP MLQVDELVAQ ASACYPDPMP LQEVLQRAGL QALAGRRYGQ LSGGQQRAVQ FAIALCGRPR VLFLDEPTTG LDIQARQAMW QAIRQLVAEG CGVLLTTHYL EEAEALAQQV VVLEQGRVLA DAPLSELRLA DRPRRIRCRS VLTVEDVQQW PGVQQVQRDG EHLQLMASPA EPVVARLLAA DAQLRELEVQ GAALVDAFLD MTREAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc cgatcgcccc gtcgctggcc cgcctgcagc aggtgcaggt gcgctaccgc gaccataccg ccctgcaggg catcgacctg caggtccgcg ctggccaggt gctggcattg ctgggccgca acggtgccgg caagagtacc gcgatcagcg tgctgttggg cctgcgccgc gccgatgccg gccaggtcga gctgctcggt ggcgacccgc agcagcgcag cagccgcctc ggcctgggcg tgatgctgca gagcaccagc ctgccgccga tgctgcaggt ggacgaactg gtggcgcagg ccagcgcctg ctatccggac ccgatgccgc tgcaggaggt gctgcagcgc gcaggcctgc aggcactggc aggccgtcgc tacggtcagc tgtccggtgg ccagcagcgc gcggtgcagt tcgccatcgc gctgtgcggc cgcccgcgcg tgctgttcct ggacgagccc accaccggcc tggacatcca ggcgcggcag gcgatgtggc aggcgatccg gcagctggtg gccgaaggct gcggcgtgct gctgaccacc cactatctgg aagaggccga agcgctggcg cagcaggtgg tggtgctgga gcagggccgg gtgctggccg acgcaccgtt gagcgagctg cggctggccg atcggccgcg ccgcatccgc tgccgcagcg tgttgactgt tgaagacgta cagcagtggc cgggcgtgca gcaggtgcag cgtgatggcg agcacctgca gttgatggca agcccggccg agccggtggt ggcgcgcctg ctggccgccg atgcacagct gcgtgagctg gaagtacagg gcgcggcact ggtcgacgct ttcctcgaca tgacccggga ggccgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at