StmaA.00630.a

Rod shape-determining protein MreB (mreB)

CENTER ID: StmaA.00630.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00630.a.B1.GE43259 Full length(StmaA.00630.a) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3829

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKKLRGMFS NDLSIDLGTA NTLIYVRGQG IVLNEPSVVA VRQDRAIGGT RSVAAVGAEA KQMLGRTPGH ITTIRPMKDG VIADFTYTEA MLKHFIKKVH KSRVLRPSPR VLVCVPAGST QVERRAIKES AEEAGARDVY LIEEPMAAAI GAGMPVTEAR GSMVIDIGGG TTEVAVISLN GIVYSASVRI GGDRFDESIT NYVRRNHGML IGEATAERIK VELGCAYPQA EVQELEISGR NLAEGVPKMI KISSNEVLEA LHEPLSGIVS AVKLALEQTP PELCADVAER GIVLTGGGAL LRDLDRLISE ETGLHVQVAD DPLTCVARGG GRALELVDMH GNEFFAPE
NT Sequence
ATGTTCAAGA AACTCCGTGG CATGTTCTCC AACGACCTGT CCATCGACCT GGGCACGGCC AACACCCTCA TCTACGTGCG CGGGCAGGGG ATCGTGCTGA ACGAGCCGTC GGTCGTGGCC GTGCGCCAGG ACCGTGCCAT CGGTGGCACC CGCTCGGTGG CAGCCGTTGG CGCCGAGGCC AAGCAGATGC TCGGCCGTAC CCCGGGCCAC ATCACCACCA TCCGCCCGAT GAAGGACGGC GTCATCGCCG ACTTCACCTA CACCGAGGCG ATGCTCAAGC ACTTCATCAA GAAGGTGCAC AAGTCGCGCG TGCTGCGCCC GAGCCCGCGC GTGCTGGTCT GCGTGCCGGC CGGTTCGACC CAGGTCGAGC GCCGCGCGAT CAAGGAATCG GCCGAGGAAG CCGGTGCCCG TGACGTGTAC CTGATCGAAG AGCCGATGGC TGCCGCGATC GGCGCCGGCA TGCCGGTCAC CGAAGCCCGT GGCTCGATGG TCATCGACAT CGGCGGCGGC ACCACCGAAG TGGCGGTGAT CTCGCTCAAC GGCATCGTCT ATTCGGCCTC GGTCCGCATC GGCGGCGACC GCTTCGACGA ATCCATCACC AACTACGTGC GCCGCAACCA CGGCATGCTG ATCGGTGAAG CCACCGCCGA GCGCATCAAG GTCGAGCTGG GTTGTGCCTA CCCGCAGGCC GAGGTGCAGG AGCTGGAGAT CTCCGGCCGC AACCTCGCCG AGGGCGTGCC GAAGATGATC AAGATCAGCT CCAACGAGGT GCTTGAAGCC CTGCACGAAC CGCTGTCGGG CATCGTCAGC GCGGTCAAGC TGGCGCTGGA GCAGACCCCG CCGGAACTGT GTGCCGACGT TGCCGAGCGC GGCATCGTGC TGACCGGCGG CGGCGCCCTG CTGCGTGACC TGGACCGCCT GATCTCCGAG GAAACCGGCC TGCACGTGCA GGTGGCCGAC GATCCGCTGA CCTGTGTGGC CCGCGGTGGC GGTCGTGCGC TGGAACTGGT CGACATGCAC GGCAACGAGT TCTTTGCGCC GGAA
Details for StmaA.00630.a.B1.GE43259
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 38kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 60
PERCENT COVERAGE: 23
Validated AA Sequence
MAHHHHHHMV DKLPGLFSND LXIXLGTSNT LXRXXXRXMV VNKASVVAVR QDXGMXDSRT VVTVRPKAKX MLGRTPGRIT TIX
Validated NT Sequence
acgactcact atagggagan cacaacggtt tccctctaga aataattttg tttaacttta ngaaggagat ataccatggc tcaccaccac caccaccata tggtcgacaa actccctggc ctgttctcca acgacctgnc catcnacctg ggcacgtcca acaccctcnt gcgcngcnnc gnncgggnga tggtggtgaa caaggcgtcg gtcgtggccg tgcgccagga cgncggcatg antgactccc gcacggtggt aaccgttcgg cccaaggcca agnagatgct cggccgnacg ccgggccgca tcaccaccat cnggtgatga aggacggcnt catcgccgac ttgcctanac caanncgatg ctctngcttc aggtgaaggt gcacggnncg ggccagntgc acccnanccc ncncgtgctg gtntgcaagn cgncggttcg acccaggtcc ngcgcnntgc gatcaaggan tcgaccgagg aagtggnngc ccagacatgt acatgatcct gnagccgatg gctgcgcgcc cgcngngccn ggatgggggn gncngaannn cnnggctcca tggtcntcga catcggcggc ggnanctgng agttggnggt gatctcgngt caangnnatc ntntattcng cctcgatccn catcnnnngn gaccgctgtc tagannntca tcaccnncnn cgtgggccan annnncggna tgctnatnnn ngaagccnnc gctgnancgc atnnnngtcg anctgtgtct gngcntacct gnngnnnang tgnnngatct gtggatctcn gngctgnacc tcgncagnng cgtgtgnnna tcatgnngat cnngtaccna ganangtncn ttgaannncn tgangaagnn ntgtnnnncn tcgncnncng ggnnnnannn ngnncntgga nctgtancna gncgnanctg nntnccnann nncnnagnnc ngcnngntga cngncnngna ncncccngct gnctganana nngnncngnn tgatcnncna annnaancgn ngcnagnnnn nnnnnngngg gcnnnngnnn ccngaantnn ncnngnnnnn nagnngnnnn nccnnnnncn gnnctgatnn nnnnnngnna nnnnnnntnn tnnnnncatc nnnannta
Expected Protein Sequence
MAHHHHHHMF KKLRGMFSND LSIDLGTANT LIYVRGQGIV LNEPSVVAVR QDRAIGGTRS VAAVGAEAKQ MLGRTPGHIT TIRPMKDGVI ADFTYTEAML KHFIKKVHKS RVLRPSPRVL VCVPAGSTQV ERRAIKESAE EAGARDVYLI EEPMAAAIGA GMPVTEARGS MVIDIGGGTT EVAVISLNGI VYSASVRIGG DRFDESITNY VRRNHGMLIG EATAERIKVE LGCAYPQAEV QELEISGRNL AEGVPKMIKI SSNEVLEALH EPLSGIVSAV KLALEQTPPE LCADVAERGI VLTGGGALLR DLDRLISEET GLHVQVADDP LTCVARGGGR ALELVDMHGN EFFAPE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttca agaaactccg tggcatgttc tccaacgacc tgtccatcga cctgggcacg gccaacaccc tcatctacgt gcgcgggcag gggatcgtgc tgaacgagcc gtcggtcgtg gccgtgcgcc aggaccgtgc catcggtggc acccgctcgg tggcagccgt tggcgccgag gccaagcaga tgctcggccg taccccgggc cacatcacca ccatccgccc gatgaaggac ggcgtcatcg ccgacttcac ctacaccgag gcgatgctca agcacttcat caagaaggtg cacaagtcgc gcgtgctgcg cccgagcccg cgcgtgctgg tctgcgtgcc ggccggttcg acccaggtcg agcgccgcgc gatcaaggaa tcggccgagg aagccggtgc ccgtgacgtg tacctgatcg aagagccgat ggctgccgcg atcggcgccg gcatgccggt caccgaagcc cgtggctcga tggtcatcga catcggcggc ggcaccaccg aagtggcggt gatctcgctc aacggcatcg tctattcggc ctcggtccgc atcggcggcg accgcttcga cgaatccatc accaactacg tgcgccgcaa ccacggcatg ctgatcggtg aagccaccgc cgagcgcatc aaggtcgagc tgggttgtgc ctacccgcag gccgaggtgc aggagctgga gatctccggc cgcaacctcg ccgagggcgt gccgaagatg atcaagatca gctccaacga ggtgcttgaa gccctgcacg aaccgctgtc gggcatcgtc agcgcggtca agctggcgct ggagcagacc ccgccggaac tgtgtgccga cgttgccgag cgcggcatcg tgctgaccgg cggcggcgcc ctgctgcgtg acctggaccg cctgatctcc gaggaaaccg gcctgcacgt gcaggtggcc gacgatccgc tgacctgtgt ggcccgcggt ggcggtcgtg cgctggaact ggtcgacatg cacggcaacg agttctttgc gccggaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at