StmaA.00612.a
Lysyl-tRNA synthetase (class II) (EC 6.1.1.6) (lysS)
| CENTER ID: | StmaA.00612.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00612.a.B1.GE43258 | Full length(StmaA.00612.a) | 1 | 503 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00612.a.B1.PW38745 | Full length(StmaA.00612.a) | 1 | 503 |
Structures
6WBD
DEPOSITED: 3/26/2020
DETERMINATION: XRay
CLONE: StmaA.00612.a.B1.GE43258
PROTEIN: StmaA.00612.a.B1.PW38745
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.2126 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSEATDTPPV DENKLIAERR EKLKALRGQG IAYPNDFRRE DFAGRLQEEF ADAETWTAEA LEGNGRQVKM AGRLMAKRIM GKASFAQIQD ESGRIQLFLQ GAVLGDAYTA FKGWDVGDII AVEGGLTRTK TGELSVKAES IRLLTKSLRP LPDKWHGLAD VEQRYRQRYV DLIVTPESRA VFIKRSKIIR AMRAWLDNRD FLEVETPMMH YIPGGAAAKP FTTHHNALDL DLYLRVAPEL YLKRLTVGGL ERVYEINRNF RNEGVSTRHN PEFTMMELYE AYATYNEIMD LTEGVIRDVA KAVNGGTEVE WDGAKIDLGP AFRRWRMDEA VRHHNPEISA ADCTDRDALL RHCERLKIRV KPSYGWGKLL LEIFEATVEH TLIQPTFITD HPVEVSPLAR ANDNDPGYTD RFELFVNGKE LANGFSELND PEDQAQRFQA QVAAKEGGDD EAMHYDADYI RALEYGMAPT GGLGIGVDRL VMLLTGSSSI RDVLLFPYMR PEQ
NT Sequence
ATGAGCGAAG CTACCGATAC CCCCCCCGTC GACGAAAACA AGTTGATCGC CGAGCGCCGC GAGAAACTCA AAGCGCTGCG TGGGCAGGGC ATCGCGTACC CGAACGACTT CCGTCGCGAG GACTTCGCCG GCCGCCTGCA GGAAGAATTC GCCGATGCCG AGACCTGGAC CGCCGAGGCG CTGGAAGGCA ACGGTCGCCA GGTGAAGATG GCCGGCCGCC TGATGGCCAA GCGCATCATG GGCAAGGCCA GCTTCGCGCA GATCCAGGAC GAGTCCGGCC GCATCCAGCT GTTCCTGCAG GGCGCGGTGC TGGGCGATGC CTACACGGCC TTCAAGGGCT GGGACGTGGG CGACATCATC GCCGTCGAAG GCGGCCTGAC CCGTACCAAG ACCGGCGAAC TGTCGGTCAA GGCAGAGTCG ATCCGCCTGC TGACCAAGTC GCTGCGCCCG CTGCCGGACA AGTGGCACGG CCTGGCCGAC GTCGAGCAGC GCTACCGCCA GCGTTACGTC GATCTGATCG TGACCCCGGA GTCGCGCGCG GTGTTCATCA AGCGCTCGAA GATCATCCGC GCCATGCGCG CGTGGCTGGA CAACCGCGAC TTCCTGGAAG TCGAGACGCC GATGATGCAT TACATCCCCG GCGGCGCGGC GGCCAAGCCG TTCACCACCC ATCACAACGC GCTGGACCTG GACCTGTACC TGCGCGTGGC GCCGGAGCTG TACCTCAAGC GCCTGACCGT GGGTGGCCTG GAGCGTGTGT ATGAGATCAA CCGCAACTTC CGCAATGAAG GCGTCAGCAC CCGCCACAAT CCGGAATTCA CCATGATGGA ACTGTACGAG GCCTATGCCA CGTACAACGA AATCATGGAC CTGACCGAGG GTGTGATCCG TGATGTGGCC AAGGCCGTGA ACGGTGGCAC CGAAGTGGAA TGGGACGGCG CGAAGATCGA CCTGGGCCCG GCGTTCCGTC GTTGGCGCAT GGATGAGGCA GTGCGCCACC ACAACCCGGA AATCTCGGCC GCTGATTGCA CTGACCGCGA TGCGCTGCTG CGCCATTGCG AGCGCTTGAA GATCCGCGTC AAGCCGTCCT ACGGCTGGGG CAAGCTGTTG CTGGAAATCT TCGAGGCCAC GGTCGAGCAC ACCCTGATCC AGCCGACCTT CATCACCGAC CATCCGGTCG AGGTCTCGCC GCTGGCCCGT GCCAATGACA ACGACCCGGG CTACACGGAT CGCTTCGAGC TGTTCGTCAA CGGCAAGGAG CTGGCCAACG GCTTCTCCGA ACTGAACGAC CCGGAAGACC AGGCCCAGCG CTTCCAGGCC CAGGTGGCCG CGAAGGAGGG TGGCGACGAC GAGGCGATGC ACTATGACGC CGACTACATC CGTGCGCTGG AGTACGGCAT GGCCCCGACC GGCGGCCTCG GCATCGGCGT TGACCGCCTG GTGATGCTGC TGACCGGCAG CAGCTCGATC CGTGACGTGC TGCTGTTCCC GTACATGCGT CCGGAGCAG
Details for StmaA.00612.a.B1.GE43258
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 58kDa |
| OBSERVED MW: | 58kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 55 |
| PERCENT COVERAGE: | 26 |
Validated AA Sequence
MAHHHHHHMX ETTDTPPVHX XKLIAXXXXK LXALRGRGIG YXXXFRREGF XGRLHEEFPD AETWTAQALE GNGRXVXMXG RLMAXAXWXR EASRXSRTSP VASSCSCRAR CWAMPTRPSR VGTWATSSPS KAD
Validated NT Sequence
cgactcacta tagggagacc acaacggttt ccctctagaa ataattttgt ttaactttan naagnagata taccatggct caccaccacc accaccatat gancgaaact accgataccc cccccgtcca cganancaag ttgatcgccg anngccncna gaaactcnaa gcgctgcgtg ggcggggnat tgggtaccna nncnacttcc gtcgcgaggg cttcnccggc cgcctgcatg aagaattccc cgatgccgag acctggaccg cccaggcgct ggaaggcaac ggtcgccang tgangatggn cggccgcctg atggccaang catnntggnt nagggaagct tcgcgccnat ccaggacnag tccggtcgca tccagctgtt cctgcagggc gcggtgctgg gcgatgccta cacggccttc aagggttggg acgtgggcga catcttcgcc gtcnaaggcg gactgacccg taccaagacc ggggaactgt cgggcaaggc acangcgatc cgcctgctga ccgattcgct gcgcccgctg ccggacaatg ggcacggcct gngccacgtc cnncancgct ancgncaccg ttacgtncat ctgatcgtga cccnnnatct cgcgcggtgt tcntcnaact ttcntgnatn gtccnccncc ntgngngngt ctggacancc gcgacttcct ggaagtcgag acnccnanga tgcattacat ccccggcngc ncngcggcna anccgntcac cnnnnntcaa nncccactgg acctggacct gnnnctnnnc gtngnagnnn agctgtacct caaccgcctg nnccgngggt ggtcctgnag ggtgtgtatt gaaaancntc cgtaaattcc nnaatgaagg ggtcngcncc cgncnccann cnggaantaa ccgtggatgg aactgtacna ggccnatgcc gagnnncaan caannnntgn acctgaccga nggggnnann ngggacgtgn cnnggcnggg aacggnngnn ccgantgnan gggannngnn ctgaantcgn cctgggnncn gnnntncnnn nnntggcncc gngnatgagg gnnngnccnn ncnncnccnc gnaannnncn ncntgatnnn nnnnatncnn nnanggcnga nntggnnnnt nnnanngnnn ntnataantn ccgcgnnann nnannnnnnn gnntg
Expected Protein Sequence
MAHHHHHHMS EATDTPPVDE NKLIAERREK LKALRGQGIA YPNDFRREDF AGRLQEEFAD AETWTAEALE GNGRQVKMAG RLMAKRIMGK ASFAQIQDES GRIQLFLQGA VLGDAYTAFK GWDVGDIIAV EGGLTRTKTG ELSVKAESIR LLTKSLRPLP DKWHGLADVE QRYRQRYVDL IVTPESRAVF IKRSKIIRAM RAWLDNRDFL EVETPMMHYI PGGAAAKPFT THHNALDLDL YLRVAPELYL KRLTVGGLER VYEINRNFRN EGVSTRHNPE FTMMELYEAY ATYNEIMDLT EGVIRDVAKA VNGGTEVEWD GAKIDLGPAF RRWRMDEAVR HHNPEISAAD CTDRDALLRH CERLKIRVKP SYGWGKLLLE IFEATVEHTL IQPTFITDHP VEVSPLARAN DNDPGYTDRF ELFVNGKELA NGFSELNDPE DQAQRFQAQV AAKEGGDDEA MHYDADYIRA LEYGMAPTGG LGIGVDRLVM LLTGSSSIRD VLLFPYMRPE Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg aagctaccga tacccccccc gtcgacgaaa acaagttgat cgccgagcgc cgcgagaaac tcaaagcgct gcgtgggcag ggcatcgcgt acccgaacga cttccgtcgc gaggacttcg ccggccgcct gcaggaagaa ttcgccgatg ccgagacctg gaccgccgag gcgctggaag gcaacggtcg ccaggtgaag atggccggcc gcctgatggc caagcgcatc atgggcaagg ccagcttcgc gcagatccag gacgagtccg gccgcatcca gctgttcctg cagggcgcgg tgctgggcga tgcctacacg gccttcaagg gctgggacgt gggcgacatc atcgccgtcg aaggcggcct gacccgtacc aagaccggcg aactgtcggt caaggcagag tcgatccgcc tgctgaccaa gtcgctgcgc ccgctgccgg acaagtggca cggcctggcc gacgtcgagc agcgctaccg ccagcgttac gtcgatctga tcgtgacccc ggagtcgcgc gcggtgttca tcaagcgctc gaagatcatc cgcgccatgc gcgcgtggct ggacaaccgc gacttcctgg aagtcgagac gccgatgatg cattacatcc ccggcggcgc ggcggccaag ccgttcacca cccatcacaa cgcgctggac ctggacctgt acctgcgcgt ggcgccggag ctgtacctca agcgcctgac cgtgggtggc ctggagcgtg tgtatgagat caaccgcaac ttccgcaatg aaggcgtcag cacccgccac aatccggaat tcaccatgat ggaactgtac gaggcctatg ccacgtacaa cgaaatcatg gacctgaccg agggtgtgat ccgtgatgtg gccaaggccg tgaacggtgg caccgaagtg gaatgggacg gcgcgaagat cgacctgggc ccggcgttcc gtcgttggcg catggatgag gcagtgcgcc accacaaccc ggaaatctcg gccgctgatt gcactgaccg cgatgcgctg ctgcgccatt gcgagcgctt gaagatccgc gtcaagccgt cctacggctg gggcaagctg ttgctggaaa tcttcgaggc cacggtcgag cacaccctga tccagccgac cttcatcacc gaccatccgg tcgaggtctc gccgctggcc cgtgccaatg acaacgaccc gggctacacg gatcgcttcg agctgttcgt caacggcaag gagctggcca acggcttctc cgaactgaac gacccggaag accaggccca gcgcttccag gcccaggtgg ccgcgaagga gggtggcgac gacgaggcga tgcactatga cgccgactac atccgtgcgc tggagtacgg catggccccg accggcggcc tcggcatcgg cgttgaccgc ctggtgatgc tgctgaccgg cagcagctcg atccgtgacg tgctgctgtt cccgtacatg cgtccggagc agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for StmaA.00612.a.B1.PW38745
| PURIFICATION DATe: | 10/30/2019 |
| CONCENTRATION: | 10mg/ml |
| OBSERVED MW: | 58kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 55 |
| PERCENT COVERAGE: | 26 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX ETTDTPPVHX XKLIAXXXXK LXALRGRGIG YXXXFRREGF XGRLHEEFPD AETWTAQALE GNGRXVXMXG RLMAXAXWXR EASRXSRTSP VASSCSCRAR CWAMPTRPSR VGTWATSSPS KAD
Validated NT Sequence
cgactcacta tagggagacc acaacggttt ccctctagaa ataattttgt ttaactttan naagnagata taccatggct caccaccacc accaccatat gancgaaact accgataccc cccccgtcca cganancaag ttgatcgccg anngccncna gaaactcnaa gcgctgcgtg ggcggggnat tgggtaccna nncnacttcc gtcgcgaggg cttcnccggc cgcctgcatg aagaattccc cgatgccgag acctggaccg cccaggcgct ggaaggcaac ggtcgccang tgangatggn cggccgcctg atggccaang catnntggnt nagggaagct tcgcgccnat ccaggacnag tccggtcgca tccagctgtt cctgcagggc gcggtgctgg gcgatgccta cacggccttc aagggttggg acgtgggcga catcttcgcc gtcnaaggcg gactgacccg taccaagacc ggggaactgt cgggcaaggc acangcgatc cgcctgctga ccgattcgct gcgcccgctg ccggacaatg ggcacggcct gngccacgtc cnncancgct ancgncaccg ttacgtncat ctgatcgtga cccnnnatct cgcgcggtgt tcntcnaact ttcntgnatn gtccnccncc ntgngngngt ctggacancc gcgacttcct ggaagtcgag acnccnanga tgcattacat ccccggcngc ncngcggcna anccgntcac cnnnnntcaa nncccactgg acctggacct gnnnctnnnc gtngnagnnn agctgtacct caaccgcctg nnccgngggt ggtcctgnag ggtgtgtatt gaaaancntc cgtaaattcc nnaatgaagg ggtcngcncc cgncnccann cnggaantaa ccgtggatgg aactgtacna ggccnatgcc gagnnncaan caannnntgn acctgaccga nggggnnann ngggacgtgn cnnggcnggg aacggnngnn ccgantgnan gggannngnn ctgaantcgn cctgggnncn gnnntncnnn nnntggcncc gngnatgagg gnnngnccnn ncnncnccnc gnaannnncn ncntgatnnn nnnnatncnn nnanggcnga nntggnnnnt nnnanngnnn ntnataantn ccgcgnnann nnannnnnnn gnntg
Expressed Protein Sequence
MAHHHHHHMS EATDTPPVDE NKLIAERREK LKALRGQGIA YPNDFRREDF AGRLQEEFAD AETWTAEALE GNGRQVKMAG RLMAKRIMGK ASFAQIQDES GRIQLFLQGA VLGDAYTAFK GWDVGDIIAV EGGLTRTKTG ELSVKAESIR LLTKSLRPLP DKWHGLADVE QRYRQRYVDL IVTPESRAVF IKRSKIIRAM RAWLDNRDFL EVETPMMHYI PGGAAAKPFT THHNALDLDL YLRVAPELYL KRLTVGGLER VYEINRNFRN EGVSTRHNPE FTMMELYEAY ATYNEIMDLT EGVIRDVAKA VNGGTEVEWD GAKIDLGPAF RRWRMDEAVR HHNPEISAAD CTDRDALLRH CERLKIRVKP SYGWGKLLLE IFEATVEHTL IQPTFITDHP VEVSPLARAN DNDPGYTDRF ELFVNGKELA NGFSELNDPE DQAQRFQAQV AAKEGGDDEA MHYDADYIRA LEYGMAPTGG LGIGVDRLVM LLTGSSSIRD VLLFPYMRPE Q
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG AAGCTACCGA TACCCCCCCC GTCGACGAAA ACAAGTTGAT CGCCGAGCGC CGCGAGAAAC TCAAAGCGCT GCGTGGGCAG GGCATCGCGT ACCCGAACGA CTTCCGTCGC GAGGACTTCG CCGGCCGCCT GCAGGAAGAA TTCGCCGATG CCGAGACCTG GACCGCCGAG GCGCTGGAAG GCAACGGTCG CCAGGTGAAG ATGGCCGGCC GCCTGATGGC CAAGCGCATC ATGGGCAAGG CCAGCTTCGC GCAGATCCAG GACGAGTCCG GCCGCATCCA GCTGTTCCTG CAGGGCGCGG TGCTGGGCGA TGCCTACACG GCCTTCAAGG GCTGGGACGT GGGCGACATC ATCGCCGTCG AAGGCGGCCT GACCCGTACC AAGACCGGCG AACTGTCGGT CAAGGCAGAG TCGATCCGCC TGCTGACCAA GTCGCTGCGC CCGCTGCCGG ACAAGTGGCA CGGCCTGGCC GACGTCGAGC AGCGCTACCG CCAGCGTTAC GTCGATCTGA TCGTGACCCC GGAGTCGCGC GCGGTGTTCA TCAAGCGCTC GAAGATCATC CGCGCCATGC GCGCGTGGCT GGACAACCGC GACTTCCTGG AAGTCGAGAC GCCGATGATG CATTACATCC CCGGCGGCGC GGCGGCCAAG CCGTTCACCA CCCATCACAA CGCGCTGGAC CTGGACCTGT ACCTGCGCGT GGCGCCGGAG CTGTACCTCA AGCGCCTGAC CGTGGGTGGC CTGGAGCGTG TGTATGAGAT CAACCGCAAC TTCCGCAATG AAGGCGTCAG CACCCGCCAC AATCCGGAAT TCACCATGAT GGAACTGTAC GAGGCCTATG CCACGTACAA CGAAATCATG GACCTGACCG AGGGTGTGAT CCGTGATGTG GCCAAGGCCG TGAACGGTGG CACCGAAGTG GAATGGGACG GCGCGAAGAT CGACCTGGGC CCGGCGTTCC GTCGTTGGCG CATGGATGAG GCAGTGCGCC ACCACAACCC GGAAATCTCG GCCGCTGATT GCACTGACCG CGATGCGCTG CTGCGCCATT GCGAGCGCTT GAAGATCCGC GTCAAGCCGT CCTACGGCTG GGGCAAGCTG TTGCTGGAAA TCTTCGAGGC CACGGTCGAG CACACCCTGA TCCAGCCGAC CTTCATCACC GACCATCCGG TCGAGGTCTC GCCGCTGGCC CGTGCCAATG ACAACGACCC GGGCTACACG GATCGCTTCG AGCTGTTCGT CAACGGCAAG GAGCTGGCCA ACGGCTTCTC CGAACTGAAC GACCCGGAAG ACCAGGCCCA GCGCTTCCAG GCCCAGGTGG CCGCGAAGGA GGGTGGCGAC GACGAGGCGA TGCACTATGA CGCCGACTAC ATCCGTGCGC TGGAGTACGG CATGGCCCCG ACCGGCGGCC TCGGCATCGG CGTTGACCGC CTGGTGATGC TGCTGACCGG CAGCAGCTCG ATCCGTGACG TGCTGCTGTT CCCGTACATG CGTCCGGAGC AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT