StmaA.00571.a

tRNA-5-carboxymethylaminomethyl-2-thiouridine(34) synthesis protein MnmE (trmE)

CENTER ID: StmaA.00571.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00571.a.B1.GE44003 Full length(StmaA.00571.a) 1 449
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.4425

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDAIRTDTI VAIASAPGAG GVGLLRLSGP RAAAIANALG APALRPRHAH YARLRDADGE VIDDGIVLWF PAPNSFTGEE VVELQGHGSP VLLQQLVARC IALGARQARP GEFSERAFLN GKLDLAQAEA IADLIAAGDN RAARAARRSL DGVFSRRIDA VVEQLVLLRI HVEAAIDFAD EPLDTLGGAQ VRRGLEQARS DLALLRRDAE RGRRLRDGLH AVLIGPPNAG KSSLLNALAG SERAIVTDIA GTTRDTLRET IRLDGLELTL VDTAGLRDGG DAIEREGMRR ARVEIERTDL ALIVLDARDP AAGEAALGEA VAAVPHKVYI HNKSDLLEAL PALDDPDRVF VSAATGAGLD DLHARLRSIA SAGAGEQVDG EFSARTRHVD AIERAQEHAQ RADGELAHEH LELAAEELRL AHDALGEITG QMSADDLLGR IFSSFCIGK
NT Sequence
ATGAACGACG CGATCCGCAC CGACACCATC GTCGCCATCG CCAGCGCCCC TGGCGCCGGC GGTGTCGGCC TGCTGCGCCT GTCCGGCCCG CGTGCTGCGG CCATCGCCAA TGCACTGGGC GCACCTGCAC TGCGTCCGCG CCACGCGCAC TACGCGCGCC TGCGTGATGC CGACGGCGAG GTCATCGACG ATGGCATCGT GCTGTGGTTC CCGGCACCGA ACAGCTTCAC CGGAGAGGAA GTGGTGGAAC TGCAGGGCCA TGGCAGCCCG GTGCTGCTGC AGCAGCTGGT CGCGCGCTGC ATCGCGCTCG GCGCGCGCCA GGCGCGGCCG GGTGAGTTCA GTGAACGGGC ATTCCTCAAC GGCAAGCTCG ACCTGGCCCA GGCCGAGGCC ATCGCCGACC TGATCGCTGC GGGCGACAAC CGCGCCGCGC GCGCGGCACG TCGCTCGCTG GATGGCGTGT TCTCGCGCCG CATCGACGCC GTGGTCGAGC AGCTGGTGCT GCTGCGCATC CACGTGGAAG CGGCCATCGA TTTTGCCGAC GAACCGCTGG ATACGCTCGG TGGCGCTCAG GTGCGGCGTG GGCTGGAACA GGCGCGCAGC GATCTGGCCC TGCTGCGCCG CGATGCCGAA CGCGGCCGTC GCCTGCGCGA TGGCCTGCAC GCGGTGCTGA TCGGTCCGCC GAACGCCGGC AAGAGTTCAC TGCTCAACGC GCTGGCCGGC AGCGAACGCG CCATCGTCAC CGACATCGCC GGTACCACCC GCGACACCCT GCGCGAAACC ATCCGCCTGG ACGGCCTGGA GCTGACCCTG GTCGACACCG CCGGCCTGCG CGACGGCGGT GACGCCATCG AACGCGAAGG CATGCGCCGC GCCCGCGTGG AAATCGAGCG CACCGACCTG GCGCTGATCG TGCTGGATGC GCGCGATCCG GCGGCCGGCG AAGCAGCGCT GGGCGAGGCC GTGGCGGCGG TGCCGCACAA GGTCTACATC CACAACAAGT CGGACCTGCT GGAGGCGTTG CCAGCACTGG ACGATCCGGA CCGCGTGTTC GTCTCGGCTG CGACCGGCGC CGGGCTCGAC GATCTGCACG CGCGTCTGCG TTCGATCGCT TCGGCCGGGG CAGGGGAGCA GGTGGACGGC GAGTTCTCCG CGCGCACGCG GCATGTGGAT GCGATTGAAC GCGCGCAGGA GCACGCGCAG CGCGCCGACG GCGAGCTGGC GCACGAGCAC CTGGAGCTGG CCGCCGAGGA ACTGCGGCTG GCGCATGACG CGCTGGGCGA GATCACCGGG CAGATGAGTG CCGATGACCT GCTGGGCAGG ATCTTCTCCA GCTTCTGCAT CGGCAAG
Details for StmaA.00571.a.B1.GE44003
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 57
PERCENT COVERAGE: 13
Validated AA Sequence
MAHHHHHHMN DAILPHIILA IGXDPGACGV GLLRRXARGX GTSPMAWPRL SWXRSTCTTR A
Validated NT Sequence
gnnnnnnnnn nnnnnnanna nacgactcnc tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttn angaaggaga tataccatgg ctcaccacca ccaccaccat atgaacgacg ccatcctgcc ccacatcatc ctcgccatcg gcnacgaccc tggcgcctgc ggcgtcggcc tgctgcgccg tncggcccgc gggntgggga catcgccaat ggcttggccg cgcctgtcct gggnncgctc cacctgcact acccgcgcct ganngatgtc gacggtcatg tgtcnacgat ggcctcatgc tgtggttccc ggccccaaca ncttcccggt agacgaangg gtggaactcc nnggccatgg natcccggtg ctgntnnagc gngtgtgctt gngcggcatc ccngtcggcg atngcccngn acggccgggt gagttcagcg aacgggcntt cctcaacggn ganctcgacc aggcccatga nnaggccatc ggcgacctga tcgctgnttg cgacaaccgc tgcgngggcg gcactcnnct cgctggatgg tgtgtttctc ccaccgcgtc gacgccntgg tcgagtannt ggtgctggtg cacatcgnct tggaatcggn ccccngnctg acnangannt gctnnaaacc ctcgatcgcg nacnngtgnt ganngtgcgn nnnnngnggc gcanngannn gcannatgtg gnnctngntg nannnnnaga ccgtcgnctg tgcgatggnc agcacgcgng gntgnannat ccacnanncn gaanganntn actgntaana cgctggncnn gnnnaaacgc nncatcagtn ncnnncntnn ncnggatcac ntgggagact ntgntnanan canncnntgg aagnnctgna ggngnactnt gacnnnactt gatgnnngnn tgacgnagtn gacgncntan nacnggnnnn cangngcnnt gnncngatga nggtaacnga cntngntnnn annnnntngg nnnnancnga tccnggngnc gnntaannnn ngccnngnan nnangccnnn ntngnnnnnn gccnnnnnng annnnnnnnn nnnnnnnnnn nnnnntnaan nnnntnnann nanantcngn nngnntncan nnnnngnnag nnnnnntcna nnnnnnnnnn nnggngcnnn ntntttnnna nnnnncnnnn nnnnnnnnt
Expected Protein Sequence
MAHHHHHHMN DAIRTDTIVA IASAPGAGGV GLLRLSGPRA AAIANALGAP ALRPRHAHYA RLRDADGEVI DDGIVLWFPA PNSFTGEEVV ELQGHGSPVL LQQLVARCIA LGARQARPGE FSERAFLNGK LDLAQAEAIA DLIAAGDNRA ARAARRSLDG VFSRRIDAVV EQLVLLRIHV EAAIDFADEP LDTLGGAQVR RGLEQARSDL ALLRRDAERG RRLRDGLHAV LIGPPNAGKS SLLNALAGSE RAIVTDIAGT TRDTLRETIR LDGLELTLVD TAGLRDGGDA IEREGMRRAR VEIERTDLAL IVLDARDPAA GEAALGEAVA AVPHKVYIHN KSDLLEALPA LDDPDRVFVS AATGAGLDDL HARLRSIASA GAGEQVDGEF SARTRHVDAI ERAQEHAQRA DGELAHEHLE LAAEELRLAH DALGEITGQM SADDLLGRIF SSFCIGK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg acgcgatccg caccgacacc atcgtcgcca tcgccagcgc ccctggcgcc ggcggtgtcg gcctgctgcg cctgtccggc ccgcgtgctg cggccatcgc caatgcactg ggcgcacctg cactgcgtcc gcgccacgcg cactacgcgc gcctgcgtga tgccgacggc gaggtcatcg acgatggcat cgtgctgtgg ttcccggcac cgaacagctt caccggagag gaagtggtgg aactgcaggg ccatggcagc ccggtgctgc tgcagcagct ggtcgcgcgc tgcatcgcgc tcggcgcgcg ccaggcgcgg ccgggtgagt tcagtgaacg ggcattcctc aacggcaagc tcgacctggc ccaggccgag gccatcgccg acctgatcgc tgcgggcgac aaccgcgccg cgcgcgcggc acgtcgctcg ctggatggcg tgttctcgcg ccgcatcgac gccgtggtcg agcagctggt gctgctgcgc atccacgtgg aagcggccat cgattttgcc gacgaaccgc tggatacgct cggtggcgct caggtgcggc gtgggctgga acaggcgcgc agcgatctgg ccctgctgcg ccgcgatgcc gaacgcggcc gtcgcctgcg cgatggcctg cacgcggtgc tgatcggtcc gccgaacgcc ggcaagagtt cactgctcaa cgcgctggcc ggcagcgaac gcgccatcgt caccgacatc gccggtacca cccgcgacac cctgcgcgaa accatccgcc tggacggcct ggagctgacc ctggtcgaca ccgccggcct gcgcgacggc ggtgacgcca tcgaacgcga aggcatgcgc cgcgcccgcg tggaaatcga gcgcaccgac ctggcgctga tcgtgctgga tgcgcgcgat ccggcggccg gcgaagcagc gctgggcgag gccgtggcgg cggtgccgca caaggtctac atccacaaca agtcggacct gctggaggcg ttgccagcac tggacgatcc ggaccgcgtg ttcgtctcgg ctgcgaccgg cgccgggctc gacgatctgc acgcgcgtct gcgttcgatc gcttcggccg gggcagggga gcaggtggac ggcgagttct ccgcgcgcac gcggcatgtg gatgcgattg aacgcgcgca ggagcacgcg cagcgcgccg acggcgagct ggcgcacgag cacctggagc tggccgccga ggaactgcgg ctggcgcatg acgcgctggg cgagatcacc gggcagatga gtgccgatga cctgctgggc aggatcttct ccagcttctg catcggcaag tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat