SSGCID: Target Page

StmaA.00462.a

Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9) (ribE)

CENTER ID:	StmaA.00462.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00462.a.B1.GE43254	Full length(StmaA.00462.a)		1	208

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.694

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFTGIIEGVG RLVAREPIGG DVRFTFNVGN LPFDNVQMGE SIAINGVCLT VIAFDTNSFQ ADASTETLGL TTLGQLGEGA VINLERAMRP TDRLGGHLVS GHVDGLGQVL SIHEDARAQR WRFGAPAALR RYIAKKGSIC VDGVSLTVNE VDDEGFEVAL IPHTVANTAF SASGVGSAVN LEIDLVARYV ERLISVPTNG QHPQGDVA

NT Sequence

TTGTTTACTG GAATCATCGA AGGCGTCGGC CGTCTGGTCG CACGCGAACC CATCGGCGGC GATGTCCGCT TCACCTTCAA TGTCGGGAAT CTGCCGTTCG ACAACGTGCA GATGGGCGAG AGCATTGCTA TCAACGGCGT CTGCCTCACC GTCATCGCAT TCGACACCAA CAGCTTCCAG GCCGATGCTT CCACCGAGAC CCTGGGCCTG ACCACGCTGG GCCAGCTGGG CGAAGGGGCG GTCATCAATC TGGAGCGCGC CATGCGGCCG ACCGACCGCC TCGGCGGCCA TCTGGTCAGC GGCCACGTCG ACGGCCTTGG CCAGGTACTG TCCATCCACG AAGACGCACG TGCCCAGCGC TGGCGTTTCG GCGCGCCGGC CGCGCTGCGC CGCTACATCG CCAAGAAGGG CTCGATCTGC GTGGACGGCG TCAGCCTCAC CGTCAACGAA GTGGACGATG AGGGCTTTGA AGTCGCACTG ATCCCGCACA CGGTGGCCAA CACCGCCTTC TCCGCCTCGG GCGTGGGTAG CGCGGTCAAT CTTGAAATCG ACCTGGTCGC CCGTTATGTG GAACGACTGA TCAGTGTGCC GACCAACGGT CAGCACCCAC AGGGAGATGT CGCA

Details for StmaA.00462.a.B1.GE43254

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	23kDa
OBSERVED MW:	23kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	62
PERCENT COVERAGE:	86

Validated AA Sequence

MAHHHHXXXX XXIEXXGRXV AREPIGGDVX XXXXXGNXXX XNXXXXXXXX XXGVCLTVXA FDTNSFQADA XXXTXXXXTL GQLGEGAVIX XEXXXXXXDX LGGXXVXGHV XGLGQVXSIH EDARAQRWRF XXPXALRXYI XKKGSXXVDG VSLXXNEVDD EGXEVXXXXH XVANTXXXAS GVGSAV

Validated NT Sequence

attgaccgcg ctacccacgc ccgaggcggn gnannnggtg ttggccaccn ngtgnnnnan nnnngngact tcanagccct catcgtccac ttcgttgang gngaggctga cgccgtccac ncanatcgag cccttcttgn cgatgtanng gcgcagcgcg nccggcncgn ngaaacgcca gcgctgggca cgtgcgtctt cgtggatgga cngtacctgg ccaaggccgn ngacgtggcc gntgaccnnn tgnccnccga ggncgtcggn ngnncncang nnnngctccn nantgatgac cgccccttcn cccagctggc ccagcgtgnt cnggnccnnn gtnncgnnnn aagcatcggc ctggaagctg ttggtgtcaa atgcnatgac ggtgaggcag acgccgntga nancnannnt ntnnnncnnn nncncgttgn nnancnncng attcccgnca tnnaagnnna annngacatc gccgccgatg ggttcgcgtg cgaccngncg nccganncnt tcgatgntnn canncnnnnn nnngtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMF TGIIEGVGRL VAREPIGGDV RFTFNVGNLP FDNVQMGESI AINGVCLTVI AFDTNSFQAD ASTETLGLTT LGQLGEGAVI NLERAMRPTD RLGGHLVSGH VDGLGQVLSI HEDARAQRWR FGAPAALRRY IAKKGSICVD GVSLTVNEVD DEGFEVALIP HTVANTAFSA SGVGSAVNLE IDLVARYVER LISVPTNGQH PQGDVA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgttta ctggaatcat cgaaggcgtc ggccgtctgg tcgcacgcga acccatcggc ggcgatgtcc gcttcacctt caatgtcggg aatctgccgt tcgacaacgt gcagatgggc gagagcattg ctatcaacgg cgtctgcctc accgtcatcg cattcgacac caacagcttc caggccgatg cttccaccga gaccctgggc ctgaccacgc tgggccagct gggcgaaggg gcggtcatca atctggagcg cgccatgcgg ccgaccgacc gcctcggcgg ccatctggtc agcggccacg tcgacggcct tggccaggta ctgtccatcc acgaagacgc acgtgcccag cgctggcgtt tcggcgcgcc ggccgcgctg cgccgctaca tcgccaagaa gggctcgatc tgcgtggacg gcgtcagcct caccgtcaac gaagtggacg atgagggctt tgaagtcgca ctgatcccgc acacggtggc caacaccgcc ttctccgcct cgggcgtggg tagcgcggtc aatcttgaaa tcgacctggt cgcccgttat gtggaacgac tgatcagtgt gccgaccaac ggtcagcacc cacagggaga tgtcgcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at