StmaA.00367.a

RNA polymerase sigma factor RpoH (rpoH)

CENTER ID: StmaA.00367.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00367.a.B1.GE43253 Full length(StmaA.00367.a) 1 282
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.4027

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANNLPIPSA LGSLDAYIGA VHQIPVLSVD DEQDLARRFR DGNDLDAARE LVHSHLRFVV HVARGYNGYG LALGDLIQEG NIGLMKAVKR FDPDMGVRLV SFAVHWIRAE MHEFILKNWR IVKVATTKAQ RKLFFNLRKS KTRLGWMNAA EVSAVAKDLN VSEREVMEME SRLSGRDIGF DAPTDEDNEH APPSPAAFLV ADDEDPSMAY EREDSEDNQM QLLREGLAEL DARSRDIIRR RWLDADSKVT LQELADEYGV SAERIRQVEA NALKKMKALF TA
NT Sequence
GTGGCAAACA ATCTCCCGAT TCCCAGTGCG CTCGGTTCGC TGGACGCCTA CATCGGTGCC GTGCACCAGA TCCCGGTGCT GTCGGTCGAT GACGAACAGG ACCTGGCCCG TCGCTTCCGC GACGGCAACG ATCTGGACGC CGCCCGCGAG CTGGTGCATT CGCACCTGCG CTTCGTGGTG CACGTGGCCC GCGGTTACAA CGGCTACGGC CTTGCCCTGG GCGATCTGAT CCAGGAAGGC AACATCGGCC TGATGAAGGC GGTCAAGCGC TTCGACCCGG ACATGGGCGT GCGCCTGGTC TCCTTCGCCG TGCACTGGAT CCGTGCAGAG ATGCACGAGT TCATCCTGAA GAACTGGCGC ATCGTGAAGG TCGCCACCAC CAAGGCGCAG CGCAAGCTGT TCTTCAACCT GCGCAAGTCG AAGACGCGCC TGGGCTGGAT GAACGCGGCG GAAGTGAGCG CGGTGGCCAA GGACCTGAAC GTGTCCGAGC GCGAAGTCAT GGAAATGGAG TCGCGCCTGT CCGGCCGCGA TATCGGTTTC GATGCGCCGA CCGACGAGGA CAACGAACAC GCACCGCCGT CGCCGGCTGC GTTCCTGGTT GCCGATGACG AAGACCCGTC GATGGCCTAC GAGCGCGAGG ACAGCGAAGA CAACCAGATG CAGCTGCTGC GCGAAGGCCT GGCGGAACTG GATGCCCGCT CGCGCGACAT CATCCGCCGT CGCTGGCTGG ACGCCGACAG CAAGGTGACG CTGCAGGAGC TGGCCGACGA GTACGGCGTG TCGGCCGAGC GCATCCGCCA GGTTGAGGCG AACGCGCTGA AGAAGATGAA GGCGCTGTTC ACCGCG
Details for StmaA.00367.a.B1.GE43253
HARVESTED ON: 6/12/2019
SEQUENCED ON: 6/18/2019
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV ANNLPIPSAL GSLDAYIGAV HQIPVLSVDD EQDLARRFRD GNDLDAAREL VHSHLRFVVH VARGYNGYGL ALGDLIQEGN IGLMKAVKRF DPDMGVRLVS FAVHWIRAEM HEFILKNWRI VKVATTKAQR KLFFNLRKSK TRLGWMNAAE VSAVAKDLNV SEREVMEMES RLSGRDIGFD APTDEDNEHA PPSPAAFLVA DDEDPSMAYE REDSEDNQMQ LLREGLAELD ARSRDIIRRR WLDADSKVTL QELADEYGVS AERIRQVEAN ALKXXKALFT A
Validated NT Sequence
tactcactta cgcggtgaac agcgccttcn tnttcttcag cgcgttcgcc tcaacctggc ggatgcgctc ggccgacacg ccgtactcgt cggccagctc ctgcagcgtc accttgctgt cggcgtccag ccagcgacgg cggatgatgt cgcgcgagcg ggcatccagt tccgccaggc cttcgcgcag cagctgcatc tggttgtctt cgctgtcctc gcgctcgtag gccatcgacg ggtcttcgtc atcggcaacc aggaacgcag ccggcgacgg cggtgcgtgt tcgttgtcct cgtcggtcgg cgcatcgaaa ccgatatcgc ggccggacag gcgcgactcc atttccatga cttcgcgctc ggacacgttc aggtccttgg ccaccgcgct cacttccgcc gcgttcatcc agcccaggcg cgtcttcgac ttgcgcaggt tgaagaacag cttgcgctgc gccttggtgg tggcgacctt cacgatgcgc cagttcttca ggatgaactc gtgcatctct gcacggatcc agtgcacggc gaaggagacc aggcgcacgc ccatgtccgg gtcgaagcgc ttgaccgcct tcatcaggcc gatgttgcct tcctggatca gatcgcccag ggcaaggccg tagccgttgt aaccgcgggc cacgtgcacc acgaagcgca ggtgcgaatg caccagctcg cgggcggcgt ccagatcgtt gccgtcgcgg aagcgacggg ccaggtcctg ttcgtcatcg accgacagca ccgggatctg gtgcacggca ccgatgtagg cgtccagcga accgagcgca ctgggaatcg ggagattgtt tgccaccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA NNLPIPSALG SLDAYIGAVH QIPVLSVDDE QDLARRFRDG NDLDAARELV HSHLRFVVHV ARGYNGYGLA LGDLIQEGNI GLMKAVKRFD PDMGVRLVSF AVHWIRAEMH EFILKNWRIV KVATTKAQRK LFFNLRKSKT RLGWMNAAEV SAVAKDLNVS EREVMEMESR LSGRDIGFDA PTDEDNEHAP PSPAAFLVAD DEDPSMAYER EDSEDNQMQL LREGLAELDA RSRDIIRRRW LDADSKVTLQ ELADEYGVSA ERIRQVEANA LKKMKALFTA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcaa acaatctccc gattcccagt gcgctcggtt cgctggacgc ctacatcggt gccgtgcacc agatcccggt gctgtcggtc gatgacgaac aggacctggc ccgtcgcttc cgcgacggca acgatctgga cgccgcccgc gagctggtgc attcgcacct gcgcttcgtg gtgcacgtgg cccgcggtta caacggctac ggccttgccc tgggcgatct gatccaggaa ggcaacatcg gcctgatgaa ggcggtcaag cgcttcgacc cggacatggg cgtgcgcctg gtctccttcg ccgtgcactg gatccgtgca gagatgcacg agttcatcct gaagaactgg cgcatcgtga aggtcgccac caccaaggcg cagcgcaagc tgttcttcaa cctgcgcaag tcgaagacgc gcctgggctg gatgaacgcg gcggaagtga gcgcggtggc caaggacctg aacgtgtccg agcgcgaagt catggaaatg gagtcgcgcc tgtccggccg cgatatcggt ttcgatgcgc cgaccgacga ggacaacgaa cacgcaccgc cgtcgccggc tgcgttcctg gttgccgatg acgaagaccc gtcgatggcc tacgagcgcg aggacagcga agacaaccag atgcagctgc tgcgcgaagg cctggcggaa ctggatgccc gctcgcgcga catcatccgc cgtcgctggc tggacgccga cagcaaggtg acgctgcagg agctggccga cgagtacggc gtgtcggccg agcgcatccg ccaggttgag gcgaacgcgc tgaagaagat gaaggcgctg ttcaccgcgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat