StmaA.00317.a
Triosephosphate isomerase (EC 5.3.1.1) (tpiA)
| CENTER ID: | StmaA.00317.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00317.a.B1.GE43252 | Full length(StmaA.00317.a) | 1 | 251 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00317.a.B1.PW38736 | Full length(StmaA.00317.a) | 1 | 251 |
Structures
6W4U
DEPOSITED: 3/11/2020
DETERMINATION: XRay
CLONE: StmaA.00317.a.B1.GE43252
PROTEIN: StmaA.00317.a.B1.PW38736
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3196 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRKIVAGNW KLHGSRQFAN ELLGQVAAGL PLEGVDVVIL PPLPYLGELV EDFGETGLAF GAQDVSSNEK GAYTGEVCAA MLVEVGARYG LVGHSERRQY HHESSELVAR KFAAAQHAGL VPVLCVGETL EQREAGQTEA VIASQLAPVL ELVGAAGFAQ AVVAYEPVWA IGTGRTATKE QAQQVHAFIR GEVARIDARI ADSLPIVYGG SVKPDNAGEL FAQPDVDGGL VGGASLVAAD FLAIARAAAA N
NT Sequence
ATGCGCCGCA AGATCGTCGC CGGAAACTGG AAGCTGCATG GCAGCCGTCA ATTCGCCAAT GAACTGCTGG GGCAGGTGGC TGCCGGGCTG CCGCTGGAGG GGGTGGACGT GGTGATCCTG CCGCCGCTGC CGTACCTGGG CGAGCTGGTC GAGGACTTCG GCGAGACCGG CCTGGCCTTT GGCGCGCAGG ACGTGAGCAG CAACGAGAAG GGCGCCTACA CCGGCGAGGT CTGCGCGGCC ATGCTGGTCG AGGTCGGGGC CCGCTATGGC CTGGTCGGCC ATTCCGAGCG CCGCCAGTAC CACCACGAGA GCAGCGAGCT GGTCGCGCGC AAGTTCGCCG CCGCCCAGCA CGCCGGCCTG GTGCCGGTGC TGTGCGTGGG CGAGACCCTG GAACAGCGCG AGGCCGGGCA GACCGAAGCG GTCATCGCCA GCCAGCTGGC ACCGGTGCTG GAGCTGGTTG GCGCGGCCGG TTTCGCCCAG GCTGTGGTCG CCTATGAGCC GGTCTGGGCC ATCGGTACCG GCCGTACCGC CACCAAGGAG CAGGCACAGC AGGTGCACGC GTTCATCCGT GGCGAAGTCG CGCGTATCGA TGCTAGAATC GCTGATTCAC TGCCCATTGT TTACGGCGGT AGCGTGAAGC CCGACAATGC CGGGGAACTG TTCGCGCAGC CGGATGTCGA TGGTGGGCTG GTCGGCGGCG CCTCCCTGGT GGCTGCGGAC TTCCTGGCCA TCGCAAGGGC GGCGGCCGCG AAC
Details for StmaA.00317.a.B1.GE43252
| HARVESTED ON: | 6/12/2019 |
| SEQUENCED ON: | 6/18/2019 |
| EXPECTED MW: | 27kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMR RKIVAGNWKL HGSRQFANEL LGQVAAGLPL EGVDVVILPP LPYLGELVED FGETGLAFGA QDVSSNEKGA YTGEVCAAML VEVGARYGLV GHSERRQYHH ESSELVARKF AAAQHAGLVP VLCVGETLEQ REAGQTEAVI ASQLAPVLEL VGAAGFAQAV VAYEPVWAIG TGRTATKEQA QQVHAFIRGE VARIDARIAD SLPIVYGGSV KPDNAGELFA QPDVDGGLVG GASLVAADFL
Validated NT Sequence
gccaggaagt ccgcagccac cagggaggcg ccgccgacca gcccaccatc gacatccggc tgcgcgaaca gttccccggc attgtcgggc ttcacgctac cgccgtaaac aatgggcagt gaatcagcga ttctagcatc gatacgcgcg acttcgccac ggatgaacgc gtgcacctgc tgtgcctgct ccttggtggc ggtacggccg gtaccgatgg cccagaccgg ctcataggcg accacagcct gggcgaaacc ggccgcgcca accagctcca gcaccggtgc cagctggctg gcgatgaccg cttcggtctg cccggcctcg cgctgttcca gggtctcgcc cacgcacagc accggcacca ggccggcgtg ctgggcggcg gcgaacttgc gcgcgaccag ctcgctgctc tcgtggtggt actggcggcg ctcggaatgg ccgaccaggc catagcgggc cccgacctcg accagcatgg ccgcgcagac ctcgccggtg taggcgccct tctcgttgct gctcacgtcc tgcgcgccaa aggccaggcc ggtctcgccg aagtcctcga ccagctcgcc caggtacggc agcggcggca ggatcaccac gtccaccccc tccagcggca gcccggcagc cacctgcccc agcagttcat tggcgaattg acggctgcca tgcagcttcc agtttccggc gacgatcttg cggcgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMR RKIVAGNWKL HGSRQFANEL LGQVAAGLPL EGVDVVILPP LPYLGELVED FGETGLAFGA QDVSSNEKGA YTGEVCAAML VEVGARYGLV GHSERRQYHH ESSELVARKF AAAQHAGLVP VLCVGETLEQ REAGQTEAVI ASQLAPVLEL VGAAGFAQAV VAYEPVWAIG TGRTATKEQA QQVHAFIRGE VARIDARIAD SLPIVYGGSV KPDNAGELFA QPDVDGGLVG GASLVAADFL AIARAAAAN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcc gcaagatcgt cgccggaaac tggaagctgc atggcagccg tcaattcgcc aatgaactgc tggggcaggt ggctgccggg ctgccgctgg agggggtgga cgtggtgatc ctgccgccgc tgccgtacct gggcgagctg gtcgaggact tcggcgagac cggcctggcc tttggcgcgc aggacgtgag cagcaacgag aagggcgcct acaccggcga ggtctgcgcg gccatgctgg tcgaggtcgg ggcccgctat ggcctggtcg gccattccga gcgccgccag taccaccacg agagcagcga gctggtcgcg cgcaagttcg ccgccgccca gcacgccggc ctggtgccgg tgctgtgcgt gggcgagacc ctggaacagc gcgaggccgg gcagaccgaa gcggtcatcg ccagccagct ggcaccggtg ctggagctgg ttggcgcggc cggtttcgcc caggctgtgg tcgcctatga gccggtctgg gccatcggta ccggccgtac cgccaccaag gagcaggcac agcaggtgca cgcgttcatc cgtggcgaag tcgcgcgtat cgatgctaga atcgctgatt cactgcccat tgtttacggc ggtagcgtga agcccgacaa tgccggggaa ctgttcgcgc agccggatgt cgatggtggg ctggtcggcg gcgcctccct ggtggctgcg gacttcctgg ccatcgcaag ggcggcggcc gcgaactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for StmaA.00317.a.B1.PW38736
| PURIFICATION DATe: | 10/22/2019 |
| CONCENTRATION: | 25.5mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 14 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMR RKIVAGNWKL HGSRQFANEL LGQVAAGLPL EGVDVVILPP LPYLGELVED FGETGLAFGA QDVSSNEKGA YTGEVCAAML VEVGARYGLV GHSERRQYHH ESSELVARKF AAAQHAGLVP VLCVGETLEQ REAGQTEAVI ASQLAPVLEL VGAAGFAQAV VAYEPVWAIG TGRTATKEQA QQVHAFIRGE VARIDARIAD SLPIVYGGSV KPDNAGELFA QPDVDGGLVG GASLVAADFL
Validated NT Sequence
gccaggaagt ccgcagccac cagggaggcg ccgccgacca gcccaccatc gacatccggc tgcgcgaaca gttccccggc attgtcgggc ttcacgctac cgccgtaaac aatgggcagt gaatcagcga ttctagcatc gatacgcgcg acttcgccac ggatgaacgc gtgcacctgc tgtgcctgct ccttggtggc ggtacggccg gtaccgatgg cccagaccgg ctcataggcg accacagcct gggcgaaacc ggccgcgcca accagctcca gcaccggtgc cagctggctg gcgatgaccg cttcggtctg cccggcctcg cgctgttcca gggtctcgcc cacgcacagc accggcacca ggccggcgtg ctgggcggcg gcgaacttgc gcgcgaccag ctcgctgctc tcgtggtggt actggcggcg ctcggaatgg ccgaccaggc catagcgggc cccgacctcg accagcatgg ccgcgcagac ctcgccggtg taggcgccct tctcgttgct gctcacgtcc tgcgcgccaa aggccaggcc ggtctcgccg aagtcctcga ccagctcgcc caggtacggc agcggcggca ggatcaccac gtccaccccc tccagcggca gcccggcagc cacctgcccc agcagttcat tggcgaattg acggctgcca tgcagcttcc agtttccggc gacgatcttg cggcgcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMR RKIVAGNWKL HGSRQFANEL LGQVAAGLPL EGVDVVILPP LPYLGELVED FGETGLAFGA QDVSSNEKGA YTGEVCAAML VEVGARYGLV GHSERRQYHH ESSELVARKF AAAQHAGLVP VLCVGETLEQ REAGQTEAVI ASQLAPVLEL VGAAGFAQAV VAYEPVWAIG TGRTATKEQA QQVHAFIRGE VARIDARIAD SLPIVYGGSV KPDNAGELFA QPDVDGGLVG GASLVAADFL AIARAAAAN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCC GCAAGATCGT CGCCGGAAAC TGGAAGCTGC ATGGCAGCCG TCAATTCGCC AATGAACTGC TGGGGCAGGT GGCTGCCGGG CTGCCGCTGG AGGGGGTGGA CGTGGTGATC CTGCCGCCGC TGCCGTACCT GGGCGAGCTG GTCGAGGACT TCGGCGAGAC CGGCCTGGCC TTTGGCGCGC AGGACGTGAG CAGCAACGAG AAGGGCGCCT ACACCGGCGA GGTCTGCGCG GCCATGCTGG TCGAGGTCGG GGCCCGCTAT GGCCTGGTCG GCCATTCCGA GCGCCGCCAG TACCACCACG AGAGCAGCGA GCTGGTCGCG CGCAAGTTCG CCGCCGCCCA GCACGCCGGC CTGGTGCCGG TGCTGTGCGT GGGCGAGACC CTGGAACAGC GCGAGGCCGG GCAGACCGAA GCGGTCATCG CCAGCCAGCT GGCACCGGTG CTGGAGCTGG TTGGCGCGGC CGGTTTCGCC CAGGCTGTGG TCGCCTATGA GCCGGTCTGG GCCATCGGTA CCGGCCGTAC CGCCACCAAG GAGCAGGCAC AGCAGGTGCA CGCGTTCATC CGTGGCGAAG TCGCGCGTAT CGATGCTAGA ATCGCTGATT CACTGCCCAT TGTTTACGGC GGTAGCGTGA AGCCCGACAA TGCCGGGGAA CTGTTCGCGC AGCCGGATGT CGATGGTGGG CTGGTCGGCG GCGCCTCCCT GGTGGCTGCG GACTTCCTGG CCATCGCAAG GGCGGCGGCC GCGAACTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T