StmaA.00252.a

GTP-binding protein EngB (engB)

CENTER ID: StmaA.00252.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00252.a.B1.GE43250 Full length(StmaA.00252.a) 1 173
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3769

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAFAGRSNAG KSSALNALTR QNALARVSKT PGRTQQLVFF QVTPEAHLVD LPGYGYAKVP LDLQAHWQAF IDKYFRTREA LKGLVVVMDI RHPLKDYDRQ MLSYAVQRGL PAHALLTKAD KLSRSQQMQS LQKVRKELQS AYGDSVSVQV FSGEDRTGVD EARGVIGGWL GLE
NT Sequence
GTGGCCTTCG CCGGCCGCTC CAACGCCGGC AAGTCCAGTG CGCTCAACGC GCTGACCCGC CAGAACGCCC TGGCCCGTGT CTCCAAGACC CCTGGCCGTA CCCAGCAGTT GGTGTTCTTC CAGGTCACCC CGGAAGCGCA TCTGGTCGAC CTGCCAGGTT ATGGCTATGC AAAGGTGCCG CTGGACCTGC AGGCGCACTG GCAGGCCTTC ATCGACAAGT ATTTCCGCAC CCGCGAAGCC CTGAAGGGGC TGGTGGTGGT GATGGACATC CGCCATCCGC TGAAGGACTA CGACCGGCAG ATGCTGTCCT ACGCCGTGCA GCGCGGCCTG CCGGCGCACG CCCTGCTGAC CAAGGCCGAC AAGCTCAGCC GCAGCCAGCA GATGCAGTCC CTGCAGAAGG TGCGCAAGGA ACTGCAATCG GCCTACGGCG ACAGCGTCAG CGTGCAGGTG TTCTCCGGCG AAGACCGTAC CGGCGTGGAC GAAGCCCGTG GCGTGATCGG CGGCTGGCTG GGGCTGGAG
Details for StmaA.00252.a.B1.GE43250
HARVESTED ON: data unavailable
SEQUENCED ON: 6/18/2019
EXPECTED MW: 20kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 27
PERCENT COVERAGE: 52
Validated AA Sequence
MAHHHHHHMA AXVLRPIGGK SSXXXXXRXP TXCSPXXXPP PRAXXGCSSX PPXNXLXSGX VMXLXGRXWR WRTXGWPAXR XXXXWITPPX AXGGX
Validated NT Sequence
antnnnacna ctcactatag ggagaccaca acggtttccc tctagaaata attttgttta actttannaa ggagatatac catggctcac caccaccacc accatatggc ggccntngtc ctncggccca tcggcggcaa gtccagtgng ntcnggncct gncgtngccc aacgncctgc tcgccgncnn cnncaccccc gccccgcgcn nnnannggtt gttcttccng gccnccngna aacnctctnn cntccggnnn ngtnatggnt ctgcnngggc gccnatggag atggcggacc nctggctggc ctgcgncccg nnncntgnnc nnntggataa ccccnccnna ggcncntggc gganactgac ntanggatgc cgttgatgan ctanntnntc acaangncat ccacnccntg cctgnncctt gncngtggac gngcagatga accnggacga cggcgtggac cagncccnga natgcctncc gtgctgaagg tgcgcncagg actgntntgc tgcnatgtgc ttatcnttcg gnccnaagtg gtccggggaa natggtgccg gngtgnacga anctattgcn ggatctgcgc ctanntcngg nnacgcnnag atagcaagga gnactcaggg ttgcatcacg tgcngatnga cncncnagtg gnntgnncgc anagcnnnna ggactagcga taaccnanng nngccnctnn annaantctt ctgcncnnna ctgnnnangg ngangaantn nntncnggat atcgacccng agcgagtcgt ccncatgtgc nnagnnnann gantngaang nntcngcgaa nntacnangg accgnntcct cncnagccgg taacananng nncnnagnnn caagnnnnta naanangnat cagcntgagt tgcnnnnnnn ntcngnttan nngnnngcac nngaggncgn angntccnaa tnnntgaatg annatcggcn tnnggctaaa ncngnacann atgccagcng ctnnngntgn tnccnatgnc ccangnannn ancanannac nnnntncnnn nctgnnnnnn nnnnnnanct tgcncnntgg ntnnngcngn nntctnnnnt ngancntgaa ctnancntan aannttnntc nnnanannna nnnnntnnnc nnnntnnnnn atannannnc nttnnnnnnn nnnnnng
Expected Protein Sequence
MAHHHHHHMA FAGRSNAGKS SALNALTRQN ALARVSKTPG RTQQLVFFQV TPEAHLVDLP GYGYAKVPLD LQAHWQAFID KYFRTREALK GLVVVMDIRH PLKDYDRQML SYAVQRGLPA HALLTKADKL SRSQQMQSLQ KVRKELQSAY GDSVSVQVFS GEDRTGVDEA RGVIGGWLGL E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggcct tcgccggccg ctccaacgcc ggcaagtcca gtgcgctcaa cgcgctgacc cgccagaacg ccctggcccg tgtctccaag acccctggcc gtacccagca gttggtgttc ttccaggtca ccccggaagc gcatctggtc gacctgccag gttatggcta tgcaaaggtg ccgctggacc tgcaggcgca ctggcaggcc ttcatcgaca agtatttccg cacccgcgaa gccctgaagg ggctggtggt ggtgatggac atccgccatc cgctgaagga ctacgaccgg cagatgctgt cctacgccgt gcagcgcggc ctgccggcgc acgccctgct gaccaaggcc gacaagctca gccgcagcca gcagatgcag tccctgcaga aggtgcgcaa ggaactgcaa tcggcctacg gcgacagcgt cagcgtgcag gtgttctccg gcgaagaccg taccggcgtg gacgaagccc gtggcgtgat cggcggctgg ctggggctgg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat