SSGCID: Target Page

StmaA.00180.a

Phosphopantetheine adenylyltransferase (EC 2.7.7.3) (coaD)

CENTER ID:	StmaA.00180.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00180.a.B1.GE43208	Full length(StmaA.00180.a)		1	169

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.1733

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVANRRIAV YPGTFDPITN GHIDLVSRAA PLFEKVVVGV AQSPSKGPAL PLEQRVQLAR GALGHHGNVE VIGFDTLLAH FVRSVQGGVL LRGLRAVSDF EYEFQMASMN RHLIPEVETL FLTPAEQHSF ISSSLVREIA RLGGDVSGFV PAAVLEALRK VREAKAAQS

NT Sequence

ATGACCGTGG CCAACCGCCG CATTGCCGTC TATCCCGGCA CGTTCGACCC GATCACCAAC GGTCATATCG ACCTGGTCAG CCGGGCCGCG CCGCTGTTTG AAAAGGTCGT GGTGGGCGTG GCGCAGAGCC CGTCCAAGGG CCCGGCGCTA CCGCTGGAGC AGCGCGTGCA GCTGGCGCGT GGCGCACTGG GCCACCATGG CAACGTCGAG GTCATCGGCT TCGACACCCT GCTGGCCCAT TTCGTCCGTT CGGTCCAGGG TGGGGTGCTG CTGCGCGGCC TGCGCGCGGT GTCCGACTTC GAGTACGAGT TCCAGATGGC CAGCATGAAC CGCCACCTGA TCCCCGAGGT CGAGACCCTG TTCCTGACCC CGGCCGAGCA GCACAGCTTC ATTTCGTCCT CGCTGGTCCG CGAGATCGCG CGCCTGGGCG GCGACGTGTC CGGCTTCGTG CCGGCCGCGG TGCTCGAAGC CCTGCGCAAG GTCCGCGAAG CGAAGGCGGC ACAGTCG

Details for StmaA.00180.a.B1.GE43208

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT VANRRIAVYP GTFDPITNGH IDLVSRAAPL FEKVVVGVAQ SPSKGPALPL EQRVQLARGA LGHHGNVEVI GFDTLLAHFV RSVQGGVLLR GLRAVSDFEY EFQMASMNRH LIPEVETLFL TPAEQHSFIS SSLVREIARL GGDVSGFVPA AVLEALRKVR EAKAAQS

Validated NT Sequence

atcttactca cttacgactg tgccgccttc gcttcgcgga ccttgcgcag ggcttcgagc accgcggccg gcacgaagcc ggacacgtcg ccgcccaggc gcgcgatctc gcggaccagc gaggacgaaa tgaagctgtg ctgctcggcc ggggtcagga acagggtctc gacctcgggg atcaggtggc ggttcatgct ggccatctgg aactcgtact cgaagtcgga caccgcgcgc aggccgcgca gcagcacccc accctggacc gaacggacga aatgggccag cagggtgtcg aagccgatga cctcgacgtt gccatggtgg cccagtgcgc cacgcgccag ctgcacgcgc tgctccagcg gtagcgccgg gcccttggac gggctctgcg ccacgcccac cacgaccttt tcaaacagcg gcgcggcccg gctgaccagg tcgatatgac cgttggtgat cgggtcgaac gtgccgggat agacggcaat gcggcggttg gccacggtca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

MAHHHHHHMT VANRRIAVYP GTFDPITNGH IDLVSRAAPL FEKVVVGVAQ SPSKGPALPL EQRVQLARGA LGHHGNVEVI GFDTLLAHFV RSVQGGVLLR GLRAVSDFEY EFQMASMNRH LIPEVETLFL TPAEQHSFIS SSLVREIARL GGDVSGFVPA AVLEALRKVR EAKAAQS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg tggccaaccg ccgcattgcc gtctatcccg gcacgttcga cccgatcacc aacggtcata tcgacctggt cagccgggcc gcgccgctgt ttgaaaaggt cgtggtgggc gtggcgcaga gcccgtccaa gggcccggcg ctaccgctgg agcagcgcgt gcagctggcg cgtggcgcac tgggccacca tggcaacgtc gaggtcatcg gcttcgacac cctgctggcc catttcgtcc gttcggtcca gggtggggtg ctgctgcgcg gcctgcgcgc ggtgtccgac ttcgagtacg agttccagat ggccagcatg aaccgccacc tgatccccga ggtcgagacc ctgttcctga ccccggccga gcagcacagc ttcatttcgt cctcgctggt ccgcgagatc gcgcgcctgg gcggcgacgt gtccggcttc gtgccggccg cggtgctcga agccctgcgc aaggtccgcg aagcgaaggc ggcacagtcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat