StmaA.00177.a

Chaperone protein DnaK (dnaK)

CENTER ID: StmaA.00177.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00177.a.B1.GE43207 Full length(StmaA.00177.a) 1 640
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1898

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGKIIGIDLG TTNSCVAIMD GGKARVIENS EGDRTTPSIV AYTKDGEVLV GASAKRQAVT NPKNTFYAVK RLIGRKFTDA EVQKDIAHVP YSILAHDNGD AWVATSDGKK MAPQEISAKV LEKMKKTAED FLGEKVTEAV ITVPAYFNDS QRQATKDAGR IAGLDVKRII NEPTAAALAY GLDKGDNKDR KIVVYDLGGG TFDVSVIEIA NVDGEKQFEV LATNGDTFLG GEDFDNRVIE YLVEEFNKDQ GIDLRKDPLA LQRLKDAAER AKIELSSAQQ TEVNLPYVTA DASGPKHLNI KLTRAKLEAL VDDLIKKSIE PCRVALNDAG LRSSDISEVI LVGGQTRMPK VQQAVTEFFG KEPRKDVNPD EAVALGAAIQ GGVLGGDVKD VLLLDVTPLS LGIETMGGVF TKIIEKNTTI PTKASQVFST AEDNQSAVTV HVLQGEREQA RFNKSLAKFD LSGIEPAPRG LPQVEVSFDI DANGILHVSA KDKKTNKEQK VEIKAGSGLS EEEIARMVAD AEANREEDKK FQELVQARNQ ADALIHGTRS AITEHGSKVG GDVIGKVEAA LADLETAMKG DDKAQIEAKS KALEEAGQSL FAAASADQGG APGADAGNAG KAQDDVVDAE FTEVKDDKKS
NT Sequence
ATGGGCAAGA TCATTGGTAT CGACCTCGGC ACCACCAACT CGTGCGTGGC GATCATGGAC GGCGGCAAGG CCCGCGTCAT CGAGAATTCG GAAGGCGATC GCACCACCCC GTCGATCGTC GCCTACACCA AGGACGGCGA AGTCCTGGTC GGTGCCTCGG CCAAGCGCCA GGCCGTGACC AACCCGAAGA ACACCTTCTA CGCGGTGAAG CGCCTGATCG GCCGCAAGTT CACCGACGCC GAAGTGCAGA AGGACATCGC GCACGTCCCG TACAGCATCC TGGCCCATGA CAATGGCGAC GCCTGGGTGG CCACCAGCGA CGGCAAGAAG ATGGCCCCGC AGGAAATCTC GGCCAAGGTG CTGGAAAAGA TGAAGAAGAC CGCCGAGGAC TTCCTCGGTG AGAAGGTCAC CGAAGCGGTC ATCACCGTGC CGGCCTACTT CAACGACAGC CAGCGCCAGG CAACCAAGGA CGCCGGCCGC ATCGCCGGCC TGGACGTCAA GCGCATCATC AACGAGCCGA CCGCGGCCGC GCTGGCCTAT GGCCTGGACA AGGGCGACAA CAAGGATCGC AAGATCGTGG TGTACGACCT GGGCGGCGGC ACCTTCGACG TCTCGGTGAT CGAGATCGCC AACGTCGACG GTGAAAAGCA GTTCGAAGTG CTGGCCACCA ACGGCGACAC GTTCCTGGGT GGCGAAGACT TCGACAACCG CGTCATCGAG TACCTGGTTG AAGAGTTCAA CAAGGACCAG GGCATCGACC TGCGCAAGGA TCCGCTGGCC CTGCAGCGCC TGAAGGATGC TGCCGAGCGC GCCAAGATCG AGCTGTCCAG CGCCCAGCAG ACCGAAGTGA ACCTGCCGTA CGTCACCGCT GACGCGTCGG GTCCGAAGCA CCTGAACATC AAGCTGACCC GCGCCAAGCT GGAAGCCCTG GTGGACGACC TGATCAAGAA GTCGATCGAG CCGTGCCGCG TCGCCCTGAA CGATGCCGGC CTGCGTTCGA GCGACATCAG CGAAGTGATC CTGGTCGGCG GCCAGACCCG CATGCCGAAG GTGCAGCAGG CGGTGACCGA GTTCTTCGGC AAGGAACCGC GCAAGGACGT CAACCCGGAC GAAGCCGTGG CACTGGGTGC TGCGATCCAG GGCGGCGTGC TGGGCGGCGA CGTCAAGGAC GTGCTGCTGC TGGACGTGAC CCCGCTGTCG CTGGGCATCG AGACCATGGG CGGCGTGTTC ACCAAGATCA TCGAGAAGAA CACCACCATC CCGACCAAGG CCTCGCAGGT GTTCTCCACC GCCGAGGACA ACCAGTCGGC CGTGACCGTG CACGTGCTGC AGGGTGAGCG CGAACAGGCC CGCTTCAACA AGTCGCTGGC CAAGTTCGAC CTGTCCGGCA TCGAGCCGGC CCCGCGTGGC CTGCCGCAGG TGGAAGTGTC CTTCGACATC GACGCCAACG GCATCCTGCA CGTGTCGGCC AAGGACAAGA AGACCAACAA GGAACAGAAG GTCGAGATCA AGGCCGGTTC GGGCCTGTCC GAGGAAGAGA TCGCGCGCAT GGTCGCCGAC GCGGAAGCCA ACCGCGAAGA AGACAAGAAG TTCCAGGAAC TGGTGCAGGC CCGCAACCAG GCCGACGCCC TGATCCACGG CACCCGCAGC GCCATCACCG AGCACGGCAG CAAGGTCGGC GGCGATGTCA TCGGCAAGGT CGAGGCGGCC CTGGCCGACC TGGAAACCGC GATGAAGGGT GACGACAAGG CACAGATCGA AGCCAAGTCG AAGGCACTGG AAGAAGCTGG CCAGTCGCTG TTCGCCGCTG CTTCGGCCGA CCAGGGCGGT GCCCCGGGTG CCGACGCCGG CAATGCCGGC AAGGCGCAGG ACGACGTGGT CGACGCCGAG TTCACCGAAG TCAAGGACGA CAAGAAGTCC
Details for StmaA.00177.a.B1.GE43207
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 69kDa
OBSERVED MW: 69kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG KIIGIDLGTT NSCVAIMDGG KARVIENSEG DRTTPSIVAY TKDGEVLVGA SAKRQAVTNP KNTFYAVKRL IGRKFTDAEV QKDIAHVPYS ILAHDNGDAW VATSDGKKMA PQEISAKVLE KMKKTAEDFL GEKVTEAVIT VPAYFNDSQR QATKDAGRIA GLDVKRIINE PTAAALAYGL DKGDNKDRKI VVYDLGGGTF DVSVIEIANV DGEKQFEVLA TNGDTFLGGE DFDNRVIEYL VEEFNKDQGI DLRKDPLALQ RLKDAAERAK IELSSAQQTE VNLPYVTADA SGPKHLNIKL TRAKLEALVD DLIKKSIEPC RVALNDAGLR SSDISEVILV GGQTRMPKVQ QAVTEFFGKE PRKDVNPDEA VALGAAIQGG VLGGDVKDVL LLDVTPLSLG IETMGGVFTK IIEKNTTIPT KASQVFSTAE DNQSAVTVHV LQGEREQARF NKSLAKFDLS GIEPAPRGLP QVEVSFDIDA NGILHVSAKD KKTNKEQKVE IKAGSGLSEE EIARMVADAE ANREEDKKFQ ELVQARNQAD ALIHGTRSAI TEHGSKVGGD VIGKVEAALA DLETAMKGDD KAQIEAKSKA LEEAGQSLFA AASADQGGAP GADAGNAGKA QDDVVDAEFT EVKDDKKS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca agatcattgg tatcgacctc ggcaccacca actcgtgcgt ggcgatcatg gacggcggca aggcccgcgt catcgagaat tcggaaggcg atcgcaccac cccgtcgatc gtcgcctaca ccaaggacgg cgaagtcctg gtcggtgcct cggccaagcg ccaggccgtg accaacccga agaacacctt ctacgcggtg aagcgcctga tcggccgcaa gttcaccgac gccgaagtgc agaaggacat cgcgcacgtc ccgtacagca tcctggccca tgacaatggc gacgcctggg tggccaccag cgacggcaag aagatggccc cgcaggaaat ctcggccaag gtgctggaaa agatgaagaa gaccgccgag gacttcctcg gtgagaaggt caccgaagcg gtcatcaccg tgccggccta cttcaacgac agccagcgcc aggcaaccaa ggacgccggc cgcatcgccg gcctggacgt caagcgcatc atcaacgagc cgaccgcggc cgcgctggcc tatggcctgg acaagggcga caacaaggat cgcaagatcg tggtgtacga cctgggcggc ggcaccttcg acgtctcggt gatcgagatc gccaacgtcg acggtgaaaa gcagttcgaa gtgctggcca ccaacggcga cacgttcctg ggtggcgaag acttcgacaa ccgcgtcatc gagtacctgg ttgaagagtt caacaaggac cagggcatcg acctgcgcaa ggatccgctg gccctgcagc gcctgaagga tgctgccgag cgcgccaaga tcgagctgtc cagcgcccag cagaccgaag tgaacctgcc gtacgtcacc gctgacgcgt cgggtccgaa gcacctgaac atcaagctga cccgcgccaa gctggaagcc ctggtggacg acctgatcaa gaagtcgatc gagccgtgcc gcgtcgccct gaacgatgcc ggcctgcgtt cgagcgacat cagcgaagtg atcctggtcg gcggccagac ccgcatgccg aaggtgcagc aggcggtgac cgagttcttc ggcaaggaac cgcgcaagga cgtcaacccg gacgaagccg tggcactggg tgctgcgatc cagggcggcg tgctgggcgg cgacgtcaag gacgtgctgc tgctggacgt gaccccgctg tcgctgggca tcgagaccat gggcggcgtg ttcaccaaga tcatcgagaa gaacaccacc atcccgacca aggcctcgca ggtgttctcc accgccgagg acaaccagtc ggccgtgacc gtgcacgtgc tgcagggtga gcgcgaacag gcccgcttca acaagtcgct ggccaagttc gacctgtccg gcatcgagcc ggccccgcgt ggcctgccgc aggtggaagt gtccttcgac atcgacgcca acggcatcct gcacgtgtcg gccaaggaca agaagaccaa caaggaacag aaggtcgaga tcaaggccgg ttcgggcctg tccgaggaag agatcgcgcg catggtcgcc gacgcggaag ccaaccgcga agaagacaag aagttccagg aactggtgca ggcccgcaac caggccgacg ccctgatcca cggcacccgc agcgccatca ccgagcacgg cagcaaggtc ggcggcgatg tcatcggcaa ggtcgaggcg gccctggccg acctggaaac cgcgatgaag ggtgacgaca aggcacagat cgaagccaag tcgaaggcac tggaagaagc tggccagtcg ctgttcgccg ctgcttcggc cgaccagggc ggtgccccgg gtgccgacgc cggcaatgcc ggcaaggcgc aggacgacgt ggtcgacgcc gagttcaccg aagtcaagga cgacaagaag tcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat