StmaA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) (ispD)

CENTER ID: StmaA.00168.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00168.a.B1.GE43205 Full length(StmaA.00168.a) 1 232
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00168.a.B1.PS38607 Full length(StmaA.00168.a) 1 232
External Resources
RESOURCE REFERENCE ID
PATRIC ID: fig|522373.3.peg.1642
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAAIWVVVP AAGRGTRFGA PLPKQYLQAG GQILLAHTLD ALLAHPAVAG AMVVIGQDDA DWPGWNEWAG KPVLTCIGGA TRAASVLAGL QALPETVRAD EFVLVHDAAR PNLSPADLGR LLEVGRTDPV GAILAAPVRD TLKRAGDDGG IDGTEPRERL WRALTPQLFR RHQLSRALSD AAAAGVEVTD EAMAMERQGQ RPLLVEGSED NFKVTTPADL DRFEFVLSRR AG
NT Sequence
ATGAGTGCGG CCATCTGGGT CGTGGTTCCG GCTGCGGGCC GTGGCACCCG CTTCGGCGCG CCGTTGCCCA AGCAATACCT GCAGGCAGGG GGACAGATCC TGCTTGCCCA TACCCTGGAC GCCCTGCTGG CGCACCCGGC CGTGGCCGGG GCGATGGTGG TGATCGGCCA GGACGACGCC GACTGGCCCG GCTGGAACGA GTGGGCGGGC AAGCCGGTGC TGACCTGCAT CGGTGGCGCG ACCCGTGCCG CTTCGGTGCT GGCTGGCCTG CAGGCGCTGC CGGAGACGGT GCGCGCTGAT GAGTTCGTAC TGGTCCACGA TGCCGCGCGG CCGAACCTGT CGCCGGCCGA TCTCGGCCGC CTGCTTGAAG TCGGTCGTAC CGACCCGGTG GGGGCGATCC TGGCCGCACC GGTACGTGAC ACGCTCAAGC GTGCAGGCGA CGACGGTGGC ATCGATGGCA CCGAGCCGCG TGAGCGCCTC TGGCGTGCAC TGACCCCGCA GCTGTTCCGC CGCCATCAGC TCAGCCGCGC GCTGTCCGAC GCCGCTGCCG CCGGTGTTGA AGTCACCGAC GAGGCGATGG CCATGGAGCG CCAGGGCCAG CGCCCGCTGC TGGTGGAAGG CAGCGAGGAC AACTTCAAGG TCACCACCCC GGCCGATCTG GACCGGTTCG AATTCGTACT TTCCCGCCGC GCCGGC
Details for StmaA.00168.a.B1.GE43205
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 26kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS AAIWVVVPAA GRGTRFGAPL PKQYLQAGGQ ILLAHTLDAL LAHPAVAGAM VVIGQDDADW PGWNEWAGKP VLTCIGGATR AASVLAGLQA LPETVRADEF VLVHDAARPN LSPADLGRLL EVGRTDPVGA ILAAPVRDTL KRAGDDGGID GTEPRERLWR ALTPQLFRRH QLSRALSDAA AAGVEVTDEA MAMERQGQRP LLVEGSEDNF KVTTPADLDR FXXXLSRRAG
Validated NT Sequence
atcttactca cttagccggc gcggcgggaa agnannaant cgaaccggtc cagatcggcc ggggtggtga ccttgaagtt gtcctcgctg ccttccacca gcagcgggcg ctggccctgg cgctccatgg ccatcgcctc gtcggtgact tcaacaccgg cggcagcggc gtcggacagc gcgcggctga gctgatggcg gcggaacagc tgcggggtca gtgcacgcca gaggcgctca cgcggctcgg tgccatcgat gccaccgtcg tcgcctgcac gcttgagcgt gtcacgtacc ggtgcggcca ggatcgcccc caccgggtcg gtacgaccga cttcaagcag gcggccgaga tcggccggcg acaggttcgg ccgcgcggca tcgtggacca gtacgaactc atcagcgcgc accgtctccg gcagcgcctg caggccagcc agcaccgaag cggcacgggt cgcgccaccg atgcaggtca gcaccggctt gcccgcccac tcgttccagc cgggccagtc ggcgtcgtcc tggccgatca ccaccatcgc cccggccacg gccgggtgcg ccagcagggc gtccagggta tgggcaagca ggatctgtcc ccctgcctgc aggtattgct tgggcaacgg cgcgccgaag cgggtgccac ggcccgcagc cggaaccacg acccagatgg ccgcactcat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS AAIWVVVPAA GRGTRFGAPL PKQYLQAGGQ ILLAHTLDAL LAHPAVAGAM VVIGQDDADW PGWNEWAGKP VLTCIGGATR AASVLAGLQA LPETVRADEF VLVHDAARPN LSPADLGRLL EVGRTDPVGA ILAAPVRDTL KRAGDDGGID GTEPRERLWR ALTPQLFRRH QLSRALSDAA AAGVEVTDEA MAMERQGQRP LLVEGSEDNF KVTTPADLDR FEFVLSRRAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtg cggccatctg ggtcgtggtt ccggctgcgg gccgtggcac ccgcttcggc gcgccgttgc ccaagcaata cctgcaggca gggggacaga tcctgcttgc ccataccctg gacgccctgc tggcgcaccc ggccgtggcc ggggcgatgg tggtgatcgg ccaggacgac gccgactggc ccggctggaa cgagtgggcg ggcaagccgg tgctgacctg catcggtggc gcgacccgtg ccgcttcggt gctggctggc ctgcaggcgc tgccggagac ggtgcgcgct gatgagttcg tactggtcca cgatgccgcg cggccgaacc tgtcgccggc cgatctcggc cgcctgcttg aagtcggtcg taccgacccg gtgggggcga tcctggccgc accggtacgt gacacgctca agcgtgcagg cgacgacggt ggcatcgatg gcaccgagcc gcgtgagcgc ctctggcgtg cactgacccc gcagctgttc cgccgccatc agctcagccg cgcgctgtcc gacgccgctg ccgccggtgt tgaagtcacc gacgaggcga tggccatgga gcgccagggc cagcgcccgc tgctggtgga aggcagcgag gacaacttca aggtcaccac cccggccgat ctggaccggt tcgaattcgt actttcccgc cgcgccggct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for StmaA.00168.a.B1.PS38607
PURIFICATION DATe: 9/24/2019
CONCENTRATION: 7.734mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS AAIWVVVPAA GRGTRFGAPL PKQYLQAGGQ ILLAHTLDAL LAHPAVAGAM VVIGQDDADW PGWNEWAGKP VLTCIGGATR AASVLAGLQA LPETVRADEF VLVHDAARPN LSPADLGRLL EVGRTDPVGA ILAAPVRDTL KRAGDDGGID GTEPRERLWR ALTPQLFRRH QLSRALSDAA AAGVEVTDEA MAMERQGQRP LLVEGSEDNF KVTTPADLDR FXXXLSRRAG
Validated NT Sequence
atcttactca cttagccggc gcggcgggaa agnannaant cgaaccggtc cagatcggcc ggggtggtga ccttgaagtt gtcctcgctg ccttccacca gcagcgggcg ctggccctgg cgctccatgg ccatcgcctc gtcggtgact tcaacaccgg cggcagcggc gtcggacagc gcgcggctga gctgatggcg gcggaacagc tgcggggtca gtgcacgcca gaggcgctca cgcggctcgg tgccatcgat gccaccgtcg tcgcctgcac gcttgagcgt gtcacgtacc ggtgcggcca ggatcgcccc caccgggtcg gtacgaccga cttcaagcag gcggccgaga tcggccggcg acaggttcgg ccgcgcggca tcgtggacca gtacgaactc atcagcgcgc accgtctccg gcagcgcctg caggccagcc agcaccgaag cggcacgggt cgcgccaccg atgcaggtca gcaccggctt gcccgcccac tcgttccagc cgggccagtc ggcgtcgtcc tggccgatca ccaccatcgc cccggccacg gccgggtgcg ccagcagggc gtccagggta tgggcaagca ggatctgtcc ccctgcctgc aggtattgct tgggcaacgg cgcgccgaag cgggtgccac ggcccgcagc cggaaccacg acccagatgg ccgcactcat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMS AAIWVVVPAA GRGTRFGAPL PKQYLQAGGQ ILLAHTLDAL LAHPAVAGAM VVIGQDDADW PGWNEWAGKP VLTCIGGATR AASVLAGLQA LPETVRADEF VLVHDAARPN LSPADLGRLL EVGRTDPVGA ILAAPVRDTL KRAGDDGGID GTEPRERLWR ALTPQLFRRH QLSRALSDAA AAGVEVTDEA MAMERQGQRP LLVEGSEDNF KVTTPADLDR FEFVLSRRAG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTG CGGCCATCTG GGTCGTGGTT CCGGCTGCGG GCCGTGGCAC CCGCTTCGGC GCGCCGTTGC CCAAGCAATA CCTGCAGGCA GGGGGACAGA TCCTGCTTGC CCATACCCTG GACGCCCTGC TGGCGCACCC GGCCGTGGCC GGGGCGATGG TGGTGATCGG CCAGGACGAC GCCGACTGGC CCGGCTGGAA CGAGTGGGCG GGCAAGCCGG TGCTGACCTG CATCGGTGGC GCGACCCGTG CCGCTTCGGT GCTGGCTGGC CTGCAGGCGC TGCCGGAGAC GGTGCGCGCT GATGAGTTCG TACTGGTCCA CGATGCCGCG CGGCCGAACC TGTCGCCGGC CGATCTCGGC CGCCTGCTTG AAGTCGGTCG TACCGACCCG GTGGGGGCGA TCCTGGCCGC ACCGGTACGT GACACGCTCA AGCGTGCAGG CGACGACGGT GGCATCGATG GCACCGAGCC GCGTGAGCGC CTCTGGCGTG CACTGACCCC GCAGCTGTTC CGCCGCCATC AGCTCAGCCG CGCGCTGTCC GACGCCGCTG CCGCCGGTGT TGAAGTCACC GACGAGGCGA TGGCCATGGA GCGCCAGGGC CAGCGCCCGC TGCTGGTGGA AGGCAGCGAG GACAACTTCA AGGTCACCAC CCCGGCCGAT CTGGACCGGT TCGAATTCGT ACTTTCCCGC CGCGCCGGCT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT