SSGCID: Target Page

StmaA.00163.a

Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20) (pheS)

CENTER ID:	StmaA.00163.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00163.a.B1.GE43203	Full length(StmaA.00163.a)		1	317

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3162

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAAAQSPDVL EQLRVALLGK SGSITSQLKQ LGALPADERK AAGEAINQAR DVLTRALGER KALLEDAALD ARLAAEAIDI TLPGRNGDRA GLHPITRTLE RITGIFGRLG YELSEGPEIE DDWHNFEALN FPPHHPARAM HDTFYFGDGR LLRTHTSGVQ VRYMGDHAPP LRMIAAGKVY RSDSDQTHSP MFHQVEGLLV DEHSTFADLK GTLAEFVRAF FERDFEMRFR PSYFPFVEPG AEVDIAWQQP DGSTRWLEVL GCGMVHPNVL RNVGIDPERY TGFAFGMGVE RFAMLRYGVN DLRAFFENDV RFLKQFA

NT Sequence

GTGGCCGCCG CACAGAGCCC CGACGTGCTG GAGCAGCTGC GCGTGGCCCT GCTTGGCAAG AGCGGCAGCA TCACCTCGCA GCTCAAGCAG CTCGGTGCCC TGCCGGCCGA CGAGCGCAAG GCCGCCGGTG AAGCGATCAA CCAGGCCCGC GACGTGCTGA CCCGCGCGCT GGGCGAGCGC AAGGCGCTGC TGGAAGACGC GGCACTGGAT GCGCGCCTGG CCGCCGAAGC CATCGACATC ACCCTGCCGG GCCGCAATGG CGACCGTGCC GGCCTGCACC CGATCACCCG CACCCTGGAG CGCATCACCG GCATCTTCGG CCGGCTGGGC TACGAGCTGT CGGAAGGGCC GGAGATCGAG GACGACTGGC ACAACTTCGA GGCGCTGAAC TTCCCGCCGC ACCACCCGGC GCGCGCCATG CACGACACCT TCTACTTCGG CGACGGCCGC CTGCTGCGTA CGCATACCTC CGGTGTGCAG GTGCGCTACA TGGGCGACCA CGCGCCGCCG CTGCGCATGA TCGCCGCCGG CAAGGTGTAC CGCAGCGACA GCGACCAGAC CCACTCGCCG ATGTTCCACC AGGTGGAAGG CCTGCTGGTC GACGAGCATT CGACCTTCGC CGACCTGAAG GGCACGCTGG CCGAGTTCGT GCGCGCGTTC TTCGAGCGCG ACTTCGAGAT GCGCTTCCGC CCCAGCTACT TCCCGTTCGT TGAACCGGGC GCGGAAGTGG ACATCGCCTG GCAGCAGCCC GATGGCAGCA CCCGCTGGCT GGAAGTGCTC GGCTGCGGCA TGGTGCATCC GAACGTGCTG CGCAATGTCG GCATCGATCC GGAGCGCTAC ACCGGCTTCG CCTTCGGCAT GGGCGTGGAG CGTTTCGCGA TGCTGCGCTA CGGCGTCAAC GACCTGCGCG CGTTCTTCGA GAACGATGTG CGGTTCCTGA AGCAGTTCGC G

Details for StmaA.00163.a.B1.GE43203

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMV AAAQSPDVLE QLRVALLGKS GSITSQLKQL GALPADERKA AGEAINQARD VLTRALGERK ALLEDAALDA RLAAEAIDIT LPGRNGDRAG LHPITRTLER ITGIFGRLGY ELSEGPEIED DWHNFEALNF PPHHPARAMH DTFYFGDGRL LRTHTSGVQV RYMGDHAPPL RMIAAGKVYR SDSDQTHSPM FHQVEGLLVD EHSTFADLKG TLAEFVRAFF ERDFEMRFRP SYFPFVEPGA EVDIAWQQPD GSTRWLEVLG CGMVHPNVLR NVGIDPERYT GFAFGMGVER FAMLRYGVND LRAFFE

Validated NT Sequence

ttctcgaaga acgcgcgcag gtcgttgacg ccgtagcgca gcatcgcgaa acgctccacg cccatgccga aggcgaagcc ggtgtagcgc tccggatcga tgccgacatt gcgcagcacg ttcggatgca ccatgccgca gccgagcact tccagccagc gggtgctgcc atcgggctgc tgccaggcga tgtccacttc cgcgcccggt tcaacgaacg ggaagtagct ggggcggaag cgcatctcga agtcgcgctc gaagaacgcg cgcacgaact cggccagcgt gcccttcagg tcggcgaagg tcgaatgctc gtcgaccagc aggccttcca cctggtggaa catcggcgag tgggtctggt cgctgtcgct gcggtacacc ttgccggcgg cgatcatgcg cagcggcggc gcgtggtcgc ccatgtagcg cacctgcaca ccggaggtat gcgtacgcag caggcggccg tcgccgaagt agaaggtgtc gtgcatggcg cgcgccgggt ggtgcggcgg gaagttcagc gcctcgaagt tgtgccagtc gtcctcgatc tccggccctt ccgacagctc gtagcccagc cggccgaaga tgccggtgat gcgctccagg gtgcgggtga tcgggtgcag gccggcacgg tcgccattgc ggcccggcag ggtgatgtcg atggcttcgg cggccaggcg cgcatccagt gccgcgtctt ccagcagcgc cttgcgctcg cccagcgcgc gggtcagcac gtcgcgggcc tggttgatcg cttcaccggc ggccttgcgc tcgtcggccg gcagggcacc gagctgcttg agctgcgagg tgatgctgcc gctcttgcca agcagggcca cgcgcagctg ctccagcacg tcggggctct gtgcggcggc caccatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMA AAQSPDVLEQ LRVALLGKSG SITSQLKQLG ALPADERKAA GEAINQARDV LTRALGERKA LLEDAALDAR LAAEAIDITL PGRNGDRAGL HPITRTLERI TGIFGRLGYE LSEGPEIEDD WHNFEALNFP PHHPARAMHD TFYFGDGRLL RTHTSGVQVR YMGDHAPPLR MIAAGKVYRS DSDQTHSPMF HQVEGLLVDE HSTFADLKGT LAEFVRAFFE RDFEMRFRPS YFPFVEPGAE VDIAWQQPDG STRWLEVLGC GMVHPNVLRN VGIDPERYTG FAFGMGVERF AMLRYGVNDL RAFFENDVRF LKQFA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggccg ccgcacagag ccccgacgtg ctggagcagc tgcgcgtggc cctgcttggc aagagcggca gcatcacctc gcagctcaag cagctcggtg ccctgccggc cgacgagcgc aaggccgccg gtgaagcgat caaccaggcc cgcgacgtgc tgacccgcgc gctgggcgag cgcaaggcgc tgctggaaga cgcggcactg gatgcgcgcc tggccgccga agccatcgac atcaccctgc cgggccgcaa tggcgaccgt gccggcctgc acccgatcac ccgcaccctg gagcgcatca ccggcatctt cggccggctg ggctacgagc tgtcggaagg gccggagatc gaggacgact ggcacaactt cgaggcgctg aacttcccgc cgcaccaccc ggcgcgcgcc atgcacgaca ccttctactt cggcgacggc cgcctgctgc gtacgcatac ctccggtgtg caggtgcgct acatgggcga ccacgcgccg ccgctgcgca tgatcgccgc cggcaaggtg taccgcagcg acagcgacca gacccactcg ccgatgttcc accaggtgga aggcctgctg gtcgacgagc attcgacctt cgccgacctg aagggcacgc tggccgagtt cgtgcgcgcg ttcttcgagc gcgacttcga gatgcgcttc cgccccagct acttcccgtt cgttgaaccg ggcgcggaag tggacatcgc ctggcagcag cccgatggca gcacccgctg gctggaagtg ctcggctgcg gcatggtgca tccgaacgtg ctgcgcaatg tcggcatcga tccggagcgc tacaccggct tcgccttcgg catgggcgtg gagcgtttcg cgatgctgcg ctacggcgtc aacgacctgc gcgcgttctt cgagaacgat gtgcggttcc tgaagcagtt cgcgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat