StmaA.00156.a
Threonyl-tRNA synthetase (EC 6.1.1.3) (thrS)
| CENTER ID: | StmaA.00156.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00156.a.B1.GE43198 | Full length(StmaA.00156.a) | 1 | 633 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00156.a.B1.PW38695 | Full length(StmaA.00156.a) | 1 | 633 |
Structures
6VU9
DEPOSITED: 2/14/2020
DETERMINATION: XRay
CLONE: StmaA.00156.a.B1.GE43198
PROTEIN: StmaA.00156.a.B1.PW38695
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3167 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MINITLPDGS RREFENPVSV MEVAQSIGAG LAKATIAGAV DGVLVDASDV IDHDASLRII TAKDEEGVEI IRHSCAHLVG HAVKQLYPDV KMVIGPVIAE GFYYDIYSER PFTPDDMAAI EKRMGELIAQ DYDVIKKMTP RAEVIEIFKA RGEDYKLRLI EDMSEDIQAM GMYYHQEYVD MCRGPHVPNT RFLKAFKLTR ISGAYWRGDA QNEQLQRIYG TAWADKKQLE AYIKRIEEAE MRDHRRIGKQ QDLFHLQEEA PGLVFWHPKG WALWQVVEQY MRKVYRNSGY GEVRCPQILD VSLWKKSGHW DNYQDNMFFT ESEKRTYAVK PMNCPGHIQV FNQGLHSYRD LPIRYGEFGS CHRNEPSGAL HGILRVRGFT QDDGHVFCTE NQIESEVTAF HQQALAVYQH FGFDEIQIKI ALRPESRLGD DATWDKAEGA LRSALTACGV EWQELPGEGA FYGPKIEYHL KDAIGRTWQL GTMQVDFMMP GRLGAEYVDE NSQKKHPVML HRAIVGSMER FLGILIEHHA GQFPAWLAPT QVVVANITDA QADYVSGVTK TLAEQGFRVS SDLRNEKIGY KIREHTLQRV PYLLVIGDRE KENGAVAVRT RSGEDLGSMS LQAFIERLHA EGA
NT Sequence
ATGATCAACA TTACTCTTCC CGACGGCAGC CGCCGCGAAT TTGAAAACCC CGTCAGCGTC ATGGAGGTCG CCCAGTCGAT CGGCGCCGGC CTGGCCAAGG CCACCATCGC CGGTGCCGTG GATGGCGTGC TGGTCGATGC CAGCGACGTC ATCGACCACG ATGCCAGCCT GCGCATCATC ACCGCCAAGG ACGAGGAGGG CGTGGAGATC ATCCGCCACT CCTGCGCCCA CCTGGTCGGC CACGCCGTCA AGCAGCTCTA CCCGGACGTG AAGATGGTGA TCGGCCCGGT GATCGCCGAA GGCTTCTATT ACGACATCTA CTCCGAGCGC CCGTTCACCC CGGACGACAT GGCCGCGATC GAGAAGCGCA TGGGCGAGCT GATCGCCCAG GACTACGACG TCATCAAGAA GATGACCCCG CGCGCCGAAG TGATCGAGAT CTTCAAGGCC CGTGGCGAGG ACTACAAGCT GCGCCTGATC GAGGACATGT CCGAGGACAT CCAGGCGATG GGCATGTACT ACCACCAGGA ATACGTGGAC ATGTGCCGTG GCCCGCACGT GCCGAACACG CGCTTCCTGA AGGCCTTCAA GCTGACCCGC ATCTCCGGCG CCTATTGGCG CGGCGACGCG CAGAACGAGC AGCTGCAGCG CATCTACGGC ACCGCCTGGG CCGACAAGAA GCAGCTCGAG GCGTACATCA AGCGCATCGA AGAAGCTGAA ATGCGCGACC ACCGCCGCAT CGGCAAGCAG CAGGACCTGT TCCACCTGCA GGAAGAAGCA CCGGGCCTGG TGTTCTGGCA CCCGAAGGGC TGGGCGCTGT GGCAGGTGGT CGAGCAGTAC ATGCGCAAGG TCTACCGCAA CAGCGGCTAC GGCGAAGTGC GCTGCCCGCA GATCCTGGAC GTGAGCCTGT GGAAGAAGTC CGGTCACTGG GACAACTACC AGGACAACAT GTTCTTCACC GAATCGGAGA AGCGCACGTA CGCGGTCAAG CCGATGAACT GCCCAGGCCA CATCCAGGTG TTCAACCAGG GCCTGCACAG CTACCGCGAC CTGCCGATCC GCTATGGTGA GTTCGGTTCG TGCCATCGCA ACGAGCCGTC CGGCGCGCTG CACGGCATCC TGCGCGTGCG TGGCTTCACC CAGGACGACG GCCACGTGTT CTGCACGGAA AACCAGATCG AGTCGGAAGT GACGGCCTTC CACCAGCAGG CGCTGGCGGT CTACCAGCAC TTCGGTTTCG ACGAGATCCA GATCAAGATC GCGCTGCGCC CGGAATCGCG CCTGGGTGAC GACGCCACTT GGGACAAGGC CGAAGGCGCG CTGCGCTCGG CGCTGACCGC CTGTGGCGTG GAATGGCAGG AGCTGCCGGG CGAGGGCGCC TTCTACGGCC CGAAGATCGA GTACCACCTG AAGGATGCCA TCGGCCGTAC CTGGCAGCTG GGCACCATGC AGGTGGACTT CATGATGCCG GGCCGCCTGG GCGCCGAGTA CGTGGACGAG AACAGCCAGA AGAAGCACCC GGTCATGCTG CACCGCGCCA TCGTCGGCTC GATGGAGCGC TTCCTGGGCA TCCTGATCGA GCACCATGCC GGCCAGTTCC CGGCTTGGCT GGCGCCGACC CAGGTGGTGG TGGCCAACAT CACCGACGCC CAGGCCGACT ACGTCTCGGG CGTGACCAAA ACCCTTGCGG AGCAAGGCTT CCGCGTCAGC TCGGATTTGC GTAACGAGAA GATCGGCTAT AAAATCCGCG AGCATACGTT GCAGCGCGTG CCCTACCTGC TGGTCATCGG TGACCGCGAG AAGGAAAATG GGGCTGTGGC GGTGCGTACG CGTTCTGGCG AAGACCTGGG CAGCATGAGC CTGCAGGCCT TCATCGAGCG GCTCCACGCC GAGGGCGCG
Details for StmaA.00156.a.B1.GE43198
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 73kDa |
| OBSERVED MW: | 73kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMI NITLPDGSRR EFENPVSVME VAQSIGAGLA KATIAGAVDG VLVDASDVID HDASLRIITA KDEEGVEIIR HSCAHLVGHA VKQLYPDVKM VIGPVIAEGF YYDIYSERPF TPDDMAAIEK RMGELIAQDY DVIKKMTPRA EVIEIFKARG EDYKLRLIED MSEDIQAMGM YYHQEYVDMC RGPHVPNTRF LKAFKLTRIS GAYWRGDAQN EQLQRIYGTA WADKKQLEAY IKRIEEAEMR DHRRIGKQQD LFHLQEEAPG LVFWHPKGWA LWQVVEQYMR KVYRNSGYGE VRCPQILDVS LWKKSGHWDN YQDNMFFTES EKRTYAVKPM NCPGHIQVFN QGLHSYRDLP IRYGEFGSCH RNEPSGALHG ILRVRGFTQD DGHVFCTENQ IESEVTAFHQ QALAVYQHFG FDEIQIKIAL RPESRLGDDA TWDKAEGALR SALTACGVEW QELPGEGAFY GPKIEYHLKD AIGRTWQLGT MQVDFMMPGR LGAEYVDENS QKKHPVMLHR AIVGSMERFL GILIEHHAGQ FPAWLAPTQV VVANITDAQA DYVSGVTKTL AEQGFRVSSD LRNEKIGYKI REHTLQRVPY LLVIGDREKE NGAVAVRTRS GEDLGSMSLQ AFIERLHAEG A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca acattactct tcccgacggc agccgccgcg aatttgaaaa ccccgtcagc gtcatggagg tcgcccagtc gatcggcgcc ggcctggcca aggccaccat cgccggtgcc gtggatggcg tgctggtcga tgccagcgac gtcatcgacc acgatgccag cctgcgcatc atcaccgcca aggacgagga gggcgtggag atcatccgcc actcctgcgc ccacctggtc ggccacgccg tcaagcagct ctacccggac gtgaagatgg tgatcggccc ggtgatcgcc gaaggcttct attacgacat ctactccgag cgcccgttca ccccggacga catggccgcg atcgagaagc gcatgggcga gctgatcgcc caggactacg acgtcatcaa gaagatgacc ccgcgcgccg aagtgatcga gatcttcaag gcccgtggcg aggactacaa gctgcgcctg atcgaggaca tgtccgagga catccaggcg atgggcatgt actaccacca ggaatacgtg gacatgtgcc gtggcccgca cgtgccgaac acgcgcttcc tgaaggcctt caagctgacc cgcatctccg gcgcctattg gcgcggcgac gcgcagaacg agcagctgca gcgcatctac ggcaccgcct gggccgacaa gaagcagctc gaggcgtaca tcaagcgcat cgaagaagct gaaatgcgcg accaccgccg catcggcaag cagcaggacc tgttccacct gcaggaagaa gcaccgggcc tggtgttctg gcacccgaag ggctgggcgc tgtggcaggt ggtcgagcag tacatgcgca aggtctaccg caacagcggc tacggcgaag tgcgctgccc gcagatcctg gacgtgagcc tgtggaagaa gtccggtcac tgggacaact accaggacaa catgttcttc accgaatcgg agaagcgcac gtacgcggtc aagccgatga actgcccagg ccacatccag gtgttcaacc agggcctgca cagctaccgc gacctgccga tccgctatgg tgagttcggt tcgtgccatc gcaacgagcc gtccggcgcg ctgcacggca tcctgcgcgt gcgtggcttc acccaggacg acggccacgt gttctgcacg gaaaaccaga tcgagtcgga agtgacggcc ttccaccagc aggcgctggc ggtctaccag cacttcggtt tcgacgagat ccagatcaag atcgcgctgc gcccggaatc gcgcctgggt gacgacgcca cttgggacaa ggccgaaggc gcgctgcgct cggcgctgac cgcctgtggc gtggaatggc aggagctgcc gggcgagggc gccttctacg gcccgaagat cgagtaccac ctgaaggatg ccatcggccg tacctggcag ctgggcacca tgcaggtgga cttcatgatg ccgggccgcc tgggcgccga gtacgtggac gagaacagcc agaagaagca cccggtcatg ctgcaccgcg ccatcgtcgg ctcgatggag cgcttcctgg gcatcctgat cgagcaccat gccggccagt tcccggcttg gctggcgccg acccaggtgg tggtggccaa catcaccgac gcccaggccg actacgtctc gggcgtgacc aaaacccttg cggagcaagg cttccgcgtc agctcggatt tgcgtaacga gaagatcggc tataaaatcc gcgagcatac gttgcagcgc gtgccctacc tgctggtcat cggtgaccgc gagaaggaaa atggggctgt ggcggtgcgt acgcgttctg gcgaagacct gggcagcatg agcctgcagg ccttcatcga gcggctccac gccgagggcg cgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for StmaA.00156.a.B1.PW38695
| PURIFICATION DATe: | 8/29/2019 |
| CONCENTRATION: | 30mg/ml |
| OBSERVED MW: | 73kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMI NITLPDGSRR EFENPVSVME VAQSIGAGLA KATIAGAVDG VLVDASDVID HDASLRIITA KDEEGVEIIR HSCAHLVGHA VKQLYPDVKM VIGPVIAEGF YYDIYSERPF TPDDMAAIEK RMGELIAQDY DVIKKMTPRA EVIEIFKARG EDYKLRLIED MSEDIQAMGM YYHQEYVDMC RGPHVPNTRF LKAFKLTRIS GAYWRGDAQN EQLQRIYGTA WADKKQLEAY IKRIEEAEMR DHRRIGKQQD LFHLQEEAPG LVFWHPKGWA LWQVVEQYMR KVYRNSGYGE VRCPQILDVS LWKKSGHWDN YQDNMFFTES EKRTYAVKPM NCPGHIQVFN QGLHSYRDLP IRYGEFGSCH RNEPSGALHG ILRVRGFTQD DGHVFCTENQ IESEVTAFHQ QALAVYQHFG FDEIQIKIAL RPESRLGDDA TWDKAEGALR SALTACGVEW QELPGEGAFY GPKIEYHLKD AIGRTWQLGT MQVDFMMPGR LGAEYVDENS QKKHPVMLHR AIVGSMERFL GILIEHHAGQ FPAWLAPTQV VVANITDAQA DYVSGVTKTL AEQGFRVSSD LRNEKIGYKI REHTLQRVPY LLVIGDREKE NGAVAVRTRS GEDLGSMSLQ AFIERLHAEG A
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA ACATTACTCT TCCCGACGGC AGCCGCCGCG AATTTGAAAA CCCCGTCAGC GTCATGGAGG TCGCCCAGTC GATCGGCGCC GGCCTGGCCA AGGCCACCAT CGCCGGTGCC GTGGATGGCG TGCTGGTCGA TGCCAGCGAC GTCATCGACC ACGATGCCAG CCTGCGCATC ATCACCGCCA AGGACGAGGA GGGCGTGGAG ATCATCCGCC ACTCCTGCGC CCACCTGGTC GGCCACGCCG TCAAGCAGCT CTACCCGGAC GTGAAGATGG TGATCGGCCC GGTGATCGCC GAAGGCTTCT ATTACGACAT CTACTCCGAG CGCCCGTTCA CCCCGGACGA CATGGCCGCG ATCGAGAAGC GCATGGGCGA GCTGATCGCC CAGGACTACG ACGTCATCAA GAAGATGACC CCGCGCGCCG AAGTGATCGA GATCTTCAAG GCCCGTGGCG AGGACTACAA GCTGCGCCTG ATCGAGGACA TGTCCGAGGA CATCCAGGCG ATGGGCATGT ACTACCACCA GGAATACGTG GACATGTGCC GTGGCCCGCA CGTGCCGAAC ACGCGCTTCC TGAAGGCCTT CAAGCTGACC CGCATCTCCG GCGCCTATTG GCGCGGCGAC GCGCAGAACG AGCAGCTGCA GCGCATCTAC GGCACCGCCT GGGCCGACAA GAAGCAGCTC GAGGCGTACA TCAAGCGCAT CGAAGAAGCT GAAATGCGCG ACCACCGCCG CATCGGCAAG CAGCAGGACC TGTTCCACCT GCAGGAAGAA GCACCGGGCC TGGTGTTCTG GCACCCGAAG GGCTGGGCGC TGTGGCAGGT GGTCGAGCAG TACATGCGCA AGGTCTACCG CAACAGCGGC TACGGCGAAG TGCGCTGCCC GCAGATCCTG GACGTGAGCC TGTGGAAGAA GTCCGGTCAC TGGGACAACT ACCAGGACAA CATGTTCTTC ACCGAATCGG AGAAGCGCAC GTACGCGGTC AAGCCGATGA ACTGCCCAGG CCACATCCAG GTGTTCAACC AGGGCCTGCA CAGCTACCGC GACCTGCCGA TCCGCTATGG TGAGTTCGGT TCGTGCCATC GCAACGAGCC GTCCGGCGCG CTGCACGGCA TCCTGCGCGT GCGTGGCTTC ACCCAGGACG ACGGCCACGT GTTCTGCACG GAAAACCAGA TCGAGTCGGA AGTGACGGCC TTCCACCAGC AGGCGCTGGC GGTCTACCAG CACTTCGGTT TCGACGAGAT CCAGATCAAG ATCGCGCTGC GCCCGGAATC GCGCCTGGGT GACGACGCCA CTTGGGACAA GGCCGAAGGC GCGCTGCGCT CGGCGCTGAC CGCCTGTGGC GTGGAATGGC AGGAGCTGCC GGGCGAGGGC GCCTTCTACG GCCCGAAGAT CGAGTACCAC CTGAAGGATG CCATCGGCCG TACCTGGCAG CTGGGCACCA TGCAGGTGGA CTTCATGATG CCGGGCCGCC TGGGCGCCGA GTACGTGGAC GAGAACAGCC AGAAGAAGCA CCCGGTCATG CTGCACCGCG CCATCGTCGG CTCGATGGAG CGCTTCCTGG GCATCCTGAT CGAGCACCAT GCCGGCCAGT TCCCGGCTTG GCTGGCGCCG ACCCAGGTGG TGGTGGCCAA CATCACCGAC GCCCAGGCCG ACTACGTCTC GGGCGTGACC AAAACCCTTG CGGAGCAAGG CTTCCGCGTC AGCTCGGATT TGCGTAACGA GAAGATCGGC TATAAAATCC GCGAGCATAC GTTGCAGCGC GTGCCCTACC TGCTGGTCAT CGGTGACCGC GAGAAGGAAA ATGGGGCTGT GGCGGTGCGT ACGCGTTCTG GCGAAGACCT GGGCAGCATG AGCCTGCAGG CCTTCATCGA GCGGCTCCAC GCCGAGGGCG CGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT