SSGCID: Target Page

StmaA.00153.a

DNA primase DnaG (dnaG)

CENTER ID:	StmaA.00153.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00153.a.B1.GE43197	Full length(StmaA.00153.a)		1	579

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00153.a.B1.PW38701	Full length(StmaA.00153.a)		1	579

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.417

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARIPDAFID DLLARTDIVE VVGSRVPLKR QGKEYAARCP FHDERSASFT VSPTKQFYHC FGCGAHGTAI SFLMNYDRLE FLDAVDELAK RAGMEVPRNE NPRSPQQQDD SRELYSALDA AARFFQKNLE SSDKARSYLD GRGVDEENRA RFQIGYAPDG YSGLRDALGK DERRMKLLDR AGLFSKNDRG HVYDKFRDRV MFPIFDRRGR VIAFGGRVFE KDDGPKYLNS PETALFHKGR ELYGLWQVRQ ANQKIERLIV VEGYMDVVSL FQFGVTQAVA TLGTATTPDH AELLFRNAPD VFFCFDGDAA GRRAGWKALE SVLPRMKDGR QAFFLFLPDG EDPDTIVRKE GAEAFNERLK QATPLSQFFF DELTREINLG TLDGKARLAE RAKPMLAQIP DGAFGDLMKQ QLAQLTGLGG TAQATRAPMP QRQPQRTIQP VAKRSLVRGA IAVLLQQPSL ALTLGGKHHF QGLRLPGVEL LLELLGLVEQ RPDISTGALL EHFDGREEQA SLHTLAAQTL PGTEASWTQE LHDAVAQLEK QLLVQRLEEL LAKQRQQGLD DTDKYELREL LKARAGLRL

NT Sequence

ATGGCCCGTA TCCCCGACGC TTTCATCGAC GACCTGCTCG CCCGCACCGA CATCGTCGAG GTGGTGGGCA GCCGCGTGCC GTTGAAGCGC CAAGGCAAGG AGTACGCCGC GCGCTGCCCG TTCCATGACG AGCGCTCGGC GTCGTTCACG GTCTCGCCCA CCAAGCAGTT CTATCACTGC TTCGGCTGCG GCGCGCACGG CACCGCGATC AGCTTCCTGA TGAACTACGA CCGCCTCGAG TTCCTCGACG CGGTGGATGA GCTGGCCAAG CGCGCCGGCA TGGAAGTACC GCGCAACGAG AACCCGCGCA GCCCGCAGCA GCAGGACGAC AGCCGCGAGC TGTATTCGGC ACTGGATGCC GCCGCCCGGT TCTTCCAGAA GAACCTGGAA AGCAGCGACA AGGCACGCAG TTACCTCGAT GGGCGTGGCG TGGACGAAGA GAACCGCGCG CGCTTCCAGA TCGGCTACGC CCCGGACGGG TACAGCGGCC TGCGCGATGC GCTGGGCAAG GACGAGCGGC GCATGAAGCT GCTCGACCGT GCCGGCCTGT TCTCCAAGAA TGATCGCGGC CACGTCTACG ACAAGTTCCG CGACCGGGTG ATGTTCCCGA TCTTCGACCG CCGTGGCCGG GTGATCGCAT TCGGCGGCCG CGTGTTCGAG AAGGACGACG GCCCGAAGTA CCTCAACTCG CCCGAAACCG CGCTGTTCCA CAAGGGCCGC GAACTGTACG GCCTGTGGCA GGTGCGCCAG GCCAACCAGA AGATCGAGCG GTTGATCGTG GTCGAGGGCT ACATGGACGT GGTCTCGCTG TTCCAGTTCG GTGTCACCCA GGCGGTGGCA ACACTGGGTA CCGCAACCAC GCCGGACCAT GCCGAGCTGC TGTTCCGCAA CGCGCCGGAC GTGTTCTTCT GCTTCGACGG CGACGCCGCC GGCCGCCGCG CCGGCTGGAA GGCGCTGGAG TCGGTGCTGC CGCGCATGAA GGACGGCCGC CAGGCCTTCT TCCTGTTCCT GCCCGATGGC GAAGACCCGG ACACCATCGT GCGCAAGGAA GGCGCCGAAG CGTTCAACGA GCGCCTGAAG CAGGCTACGC CGCTGTCGCA GTTCTTCTTC GACGAACTGA CCCGCGAGAT CAACCTGGGC ACGCTCGATG GCAAGGCACG CCTGGCCGAA CGCGCCAAGC CGATGCTGGC GCAGATTCCC GACGGCGCCT TCGGCGACCT GATGAAGCAG CAGCTGGCGC AGCTGACCGG CCTGGGCGGT ACTGCCCAGG CCACGCGCGC GCCGATGCCG CAACGCCAGC CGCAGCGGAC GATCCAGCCC GTGGCCAAGC GCAGCCTGGT GCGCGGCGCG ATCGCCGTGC TGCTGCAGCA GCCGTCGCTG GCGCTGACGC TGGGCGGCAA GCACCACTTC CAGGGCCTGC GCCTGCCCGG CGTGGAACTG CTGCTGGAGC TGCTGGGGCT GGTCGAACAG CGCCCGGACA TCAGCACCGG CGCCCTGCTG GAACACTTCG ACGGGCGCGA GGAACAGGCC TCGCTGCATA CCCTGGCCGC ACAGACGCTG CCCGGCACCG AGGCCAGCTG GACCCAGGAA CTGCACGACG CAGTGGCACA GCTGGAGAAA CAGCTGCTGG TGCAACGTCT GGAGGAGTTG TTGGCAAAGC AGCGCCAGCA GGGCCTGGAC GATACTGACA AGTACGAACT GCGCGAGCTG CTGAAGGCGC GCGCCGGACT GCGCCTG

Details for StmaA.00153.a.B1.GE43197

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	66kDa
OBSERVED MW:	66kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA RIPDAFIDDL LARTDIVEVV GSRVPLKRQG KEYAARCPFH DERSASFTVS PTKQFYHCFG CGAHGTAISF LMNYDRLEFL DAVDELAKRA GMEVPRNENP RSPQQQDDSR ELYSALDAAA RFFQKNLESS DKARSYLDGR GVDEENRARF QIGYAPDGYS GLRDALGKDE RRMKLLDRAG LFSKNDRGHV YDKFRDRVMF PIFDRRGRVI AFGGRVFEKD DGPKYLNSPE TALFHKGREL YGLWQVRQAN QKIERLIVVE GYMDVVSLFQ FGVTQAVATL GTATTPDHAE LLFRNAPDVF FCFDGDAAGR RAGWKALESV LPRMKDGRQA FFLFLPDGED PDTIVRKEGA EAFNERLKQA TPLSQFFFDE LTREINLGTL DGKARLAERA KPMLAQIPDG AFGDLMKQQL AQLTGLGGTA QATRAPMPQR QPQRTIQPVA KRSLVRGAIA VLLQQPSLAL TLGGKHHFQG LRLPGVELLL ELLGLVEQRP DISTGALLEH FDGREEQASL HTLAAQTLPG TEASWTQELH DAVAQLEKQL LVQRLEELLA KQRQQGLDDT DKYELRELLK ARAGLRL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccc gtatccccga cgctttcatc gacgacctgc tcgcccgcac cgacatcgtc gaggtggtgg gcagccgcgt gccgttgaag cgccaaggca aggagtacgc cgcgcgctgc ccgttccatg acgagcgctc ggcgtcgttc acggtctcgc ccaccaagca gttctatcac tgcttcggct gcggcgcgca cggcaccgcg atcagcttcc tgatgaacta cgaccgcctc gagttcctcg acgcggtgga tgagctggcc aagcgcgccg gcatggaagt accgcgcaac gagaacccgc gcagcccgca gcagcaggac gacagccgcg agctgtattc ggcactggat gccgccgccc ggttcttcca gaagaacctg gaaagcagcg acaaggcacg cagttacctc gatgggcgtg gcgtggacga agagaaccgc gcgcgcttcc agatcggcta cgccccggac gggtacagcg gcctgcgcga tgcgctgggc aaggacgagc ggcgcatgaa gctgctcgac cgtgccggcc tgttctccaa gaatgatcgc ggccacgtct acgacaagtt ccgcgaccgg gtgatgttcc cgatcttcga ccgccgtggc cgggtgatcg cattcggcgg ccgcgtgttc gagaaggacg acggcccgaa gtacctcaac tcgcccgaaa ccgcgctgtt ccacaagggc cgcgaactgt acggcctgtg gcaggtgcgc caggccaacc agaagatcga gcggttgatc gtggtcgagg gctacatgga cgtggtctcg ctgttccagt tcggtgtcac ccaggcggtg gcaacactgg gtaccgcaac cacgccggac catgccgagc tgctgttccg caacgcgccg gacgtgttct tctgcttcga cggcgacgcc gccggccgcc gcgccggctg gaaggcgctg gagtcggtgc tgccgcgcat gaaggacggc cgccaggcct tcttcctgtt cctgcccgat ggcgaagacc cggacaccat cgtgcgcaag gaaggcgccg aagcgttcaa cgagcgcctg aagcaggcta cgccgctgtc gcagttcttc ttcgacgaac tgacccgcga gatcaacctg ggcacgctcg atggcaaggc acgcctggcc gaacgcgcca agccgatgct ggcgcagatt cccgacggcg ccttcggcga cctgatgaag cagcagctgg cgcagctgac cggcctgggc ggtactgccc aggccacgcg cgcgccgatg ccgcaacgcc agccgcagcg gacgatccag cccgtggcca agcgcagcct ggtgcgcggc gcgatcgccg tgctgctgca gcagccgtcg ctggcgctga cgctgggcgg caagcaccac ttccagggcc tgcgcctgcc cggcgtggaa ctgctgctgg agctgctggg gctggtcgaa cagcgcccgg acatcagcac cggcgccctg ctggaacact tcgacgggcg cgaggaacag gcctcgctgc ataccctggc cgcacagacg ctgcccggca ccgaggccag ctggacccag gaactgcacg acgcagtggc acagctggag aaacagctgc tggtgcaacg tctggaggag ttgttggcaa agcagcgcca gcagggcctg gacgatactg acaagtacga actgcgcgag ctgctgaagg cgcgcgccgg actgcgcctg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat

Details for StmaA.00153.a.B1.PW38701

PURIFICATION DATe:	9/8/2019
CONCENTRATION:	5.9mg/ml
OBSERVED MW:	66kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	9
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCC GTATCCCCGA CGCTTTCATC GACGACCTGC TCGCCCGCAC CGACATCGTC GAGGTGGTGG GCAGCCGCGT GCCGTTGAAG CGCCAAGGCA AGGAGTACGC CGCGCGCTGC CCGTTCCATG ACGAGCGCTC GGCGTCGTTC ACGGTCTCGC CCACCAAGCA GTTCTATCAC TGCTTCGGCT GCGGCGCGCA CGGCACCGCG ATCAGCTTCC TGATGAACTA CGACCGCCTC GAGTTCCTCG ACGCGGTGGA TGAGCTGGCC AAGCGCGCCG GCATGGAAGT ACCGCGCAAC GAGAACCCGC GCAGCCCGCA GCAGCAGGAC GACAGCCGCG AGCTGTATTC GGCACTGGAT GCCGCCGCCC GGTTCTTCCA GAAGAACCTG GAAAGCAGCG ACAAGGCACG CAGTTACCTC GATGGGCGTG GCGTGGACGA AGAGAACCGC GCGCGCTTCC AGATCGGCTA CGCCCCGGAC GGGTACAGCG GCCTGCGCGA TGCGCTGGGC AAGGACGAGC GGCGCATGAA GCTGCTCGAC CGTGCCGGCC TGTTCTCCAA GAATGATCGC GGCCACGTCT ACGACAAGTT CCGCGACCGG GTGATGTTCC CGATCTTCGA CCGCCGTGGC CGGGTGATCG CATTCGGCGG CCGCGTGTTC GAGAAGGACG ACGGCCCGAA GTACCTCAAC TCGCCCGAAA CCGCGCTGTT CCACAAGGGC CGCGAACTGT ACGGCCTGTG GCAGGTGCGC CAGGCCAACC AGAAGATCGA GCGGTTGATC GTGGTCGAGG GCTACATGGA CGTGGTCTCG CTGTTCCAGT TCGGTGTCAC CCAGGCGGTG GCAACACTGG GTACCGCAAC CACGCCGGAC CATGCCGAGC TGCTGTTCCG CAACGCGCCG GACGTGTTCT TCTGCTTCGA CGGCGACGCC GCCGGCCGCC GCGCCGGCTG GAAGGCGCTG GAGTCGGTGC TGCCGCGCAT GAAGGACGGC CGCCAGGCCT TCTTCCTGTT CCTGCCCGAT GGCGAAGACC CGGACACCAT CGTGCGCAAG GAAGGCGCCG AAGCGTTCAA CGAGCGCCTG AAGCAGGCTA CGCCGCTGTC GCAGTTCTTC TTCGACGAAC TGACCCGCGA GATCAACCTG GGCACGCTCG ATGGCAAGGC ACGCCTGGCC GAACGCGCCA AGCCGATGCT GGCGCAGATT CCCGACGGCG CCTTCGGCGA CCTGATGAAG CAGCAGCTGG CGCAGCTGAC CGGCCTGGGC GGTACTGCCC AGGCCACGCG CGCGCCGATG CCGCAACGCC AGCCGCAGCG GACGATCCAG CCCGTGGCCA AGCGCAGCCT GGTGCGCGGC GCGATCGCCG TGCTGCTGCA GCAGCCGTCG CTGGCGCTGA CGCTGGGCGG CAAGCACCAC TTCCAGGGCC TGCGCCTGCC CGGCGTGGAA CTGCTGCTGG AGCTGCTGGG GCTGGTCGAA CAGCGCCCGG ACATCAGCAC CGGCGCCCTG CTGGAACACT TCGACGGGCG CGAGGAACAG GCCTCGCTGC ATACCCTGGC CGCACAGACG CTGCCCGGCA CCGAGGCCAG CTGGACCCAG GAACTGCACG ACGCAGTGGC ACAGCTGGAG AAACAGCTGC TGGTGCAACG TCTGGAGGAG TTGTTGGCAA AGCAGCGCCA GCAGGGCCTG GACGATACTG ACAAGTACGA ACTGCGCGAG CTGCTGAAGG CGCGCGCCGG ACTGCGCCTG TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT