StmaA.00137.a

UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase (EC 6.3.2.-) (mpl)

CENTER ID: StmaA.00137.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00137.a.B2.GE43190 mature protein 22 454
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3660

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSVHILAIA GTFMGGVAAL ARELGHTVSG SDQAIYPPMS TQLEQLGIAL DQGYRADSVP AGTDDVVIGN ALSRGNAAVE AVLDAGQRYI SGAQWLSEQV LPGRDTLAVA GTHGKTTTTT ILTWLLESAG RSPGFLIGGV AEDFGVSARL GQGREFVVEA DEYDTAFFDK RSKFVHYRPL VAILNNLEYD HADIFPDVAA IQRQFHHLVR TVPARGRLIV NGEDPHLAEV LAMGCWTPVE RFGFDPSLEW HAELLAADGS AFRVHHRGRA MGDVHWSLLG RHNVLNGLAA LAAAHAVGVA PADVIPALAR FRSVKRRMEV IGRHDGITIY DDFAHHPTAI ATTLEGLRAK VGDARIVVAM EPRSNSMRLG AHAQALAPSL ALADEVVFLH RPELAWDAAA VIAAVRGEAH AVPDTDALLA QLQASARSGD HVVFMSNGGF DGAPRRFLAA LQAR
NT Sequence
ATGAGCAGCG TACATATCCT TGCAATCGCC GGCACCTTCA TGGGCGGCGT GGCCGCCCTG GCGCGGGAAC TGGGCCATAC GGTCAGCGGC AGCGACCAGG CCATCTATCC GCCGATGTCG ACCCAGCTCG AGCAGCTGGG CATCGCCCTG GACCAGGGCT ACCGCGCCGA CAGCGTGCCG GCCGGCACCG ATGACGTGGT GATCGGCAAC GCCCTCTCGC GCGGCAATGC CGCCGTTGAG GCCGTGCTCG ACGCCGGCCA GCGCTACATT TCCGGAGCGC AATGGCTGTC CGAACAGGTA CTGCCGGGTC GCGACACCCT GGCCGTGGCA GGTACCCATG GCAAGACCAC CACCACCACC ATCCTCACCT GGCTGCTGGA AAGCGCCGGT CGTTCGCCCG GTTTCCTGAT CGGCGGCGTC GCCGAGGATT TCGGCGTCTC CGCCCGGCTG GGCCAGGGCC GCGAGTTCGT AGTCGAGGCC GACGAGTACG ACACCGCGTT CTTCGACAAG CGCAGCAAGT TCGTGCACTA CCGGCCGCTG GTGGCCATCC TCAACAACCT TGAATACGAC CACGCCGACA TCTTCCCGGA CGTGGCCGCG ATCCAGCGCC AGTTCCACCA CCTGGTGCGC ACGGTGCCGG CGCGTGGCCG ACTGATCGTC AACGGCGAGG ACCCGCATCT GGCCGAAGTG CTGGCGATGG GCTGCTGGAC CCCGGTCGAG CGCTTCGGTT TCGACCCGTC GCTGGAATGG CATGCCGAGC TGCTGGCCGC CGATGGCAGC GCCTTCCGCG TACACCACCG CGGGCGGGCA ATGGGCGATG TGCACTGGTC GTTGCTGGGC CGCCACAACG TGCTCAACGG CCTGGCTGCG CTGGCTGCCG CACATGCGGT GGGCGTGGCG CCGGCCGACG TGATTCCTGC GCTGGCGCGC TTCCGCAGCG TCAAGCGCCG CATGGAAGTG ATCGGCCGCC ATGACGGCAT CACCATCTAC GACGATTTCG CCCATCACCC GACCGCGATC GCCACCACGC TGGAAGGCTT GCGTGCCAAG GTGGGTGACG CGCGCATCGT GGTCGCGATG GAGCCGCGCA GCAATTCGAT GCGGCTGGGC GCTCACGCGC AGGCGCTGGC CCCGTCGCTG GCGTTGGCTG ACGAGGTGGT GTTCCTGCAT CGCCCGGAAC TGGCCTGGGA TGCGGCCGCG GTGATCGCCG CGGTGCGCGG CGAAGCGCAC GCGGTGCCCG ATACCGACGC GCTGCTGGCG CAGCTGCAGG CCAGCGCGCG CAGCGGCGAC CACGTGGTGT TCATGTCCAA TGGGGGCTTC GACGGCGCAC CGCGTCGCTT CCTCGCCGCG CTGCAGGCCC GA
Details for StmaA.00137.a.B2.GE43190
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 48kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMR ELGHTVSGSD QAIYPPMSTQ LEQLGIALDQ GYRADSVPAG TDDVVIGNAL SRGNAAVEAV LDAGQRYISG AQWLSEQVLP GRDTLAVAGT HGKTTTTTIL TWLLESAGRS PGFLIGGVAE DFGVSARLGQ GREFVVEADE YDTAFFDKRS KFVHYRPLVA ILNNLEYDHA DIFPDVAAIQ RQFHHLVRTV PARGRLIVNG EDPHLAEVLA MGCWTPVERF GFDPSLEWHA ELLAADGSAF RVHHRGRAMG DVHWSLLGRH NVLNGLAALA AAHAVGVAPA DVIPALARFR SVKRRMEVIG RHDGITIYDD FAHHPTAIAT TLEGLRAKVG DARIVVAMEP RSNSMRLGAH AQALAPSLAL ADEVVFLHRP ELAWDAAAVI AAVRGEAHAV PDTDALLAQL QASARSGDHV VFMSNGGFDG AP
Validated NT Sequence
acgcggtgcg ccgtcgaagc ccccattgga catgaacacc acgtggtcgc cgctgcgcgc gctggcctgc agctgcgcca gcagcgcgtc ggtatcgggc accgcgtgcg cttcgccgcg caccgcggcg atcaccgcgg ccgcatccca ggccagttcc gggcgatgca ggaacaccac ctcgtcagcc aacgccagcg acggggccag cgcctgcgcg tgagcgccca gccgcatcga attgctgcgc ggctccatcg cgaccacgat gcgcgcgtca cccaccttgg cacgcaagcc ttccagcgtg gtggcgatcg cggtcgggtg atgggcgaaa tcgtcgtaga tggtgatgcc gtcatggcgg ccgatcactt ccatgcggcg cttgacgctg cggaagcgcg ccagcgcagg aatcacgtcg gccggcgcca cgcccaccgc atgtgcggca gccagcgcag ccaggccgtt gagcacgttg tggcggccca gcaacgacca gtgcacatcg cccattgccc gcccgcggtg gtgtacgcgg aaggcgctgc catcggcggc cagcagctcg gcatgccatt ccagcgacgg gtcgaaaccg aagcgctcga ccggggtcca gcagcccatc gccagcactt cggccagatg cgggtcctcg ccgttgacga tcagtcggcc acgcgccggc accgtgcgca ccaggtggtg gaactggcgc tggatcgcgg ccacgtccgg gaagatgtcg gcgtggtcgt attcaaggtt gttgaggatg gccaccagcg gccggtagtg cacgaacttg ctgcgcttgt cgaagaacgc ggtgtcgtac tcgtcggcct cgactacgaa ctcgcggccc tggcccagcc gggcggagac gccgaaatcc tcggcgacgc cgccgatcag gaaaccgggc gaacgaccgg cgctttccag cagccaggtg aggatggtgg tggtggtggt cttgccatgg gtacctgcca cggccagggt gtcgcgaccc ggcagtacct gttcggacag ccattgcgct ccggaaatgt agcgctggcc ggcgtcgagc acggcctcaa cggcggcatt gccgcgcgag agggcgttgc cgatcaccac gtcatcggtg ccggccggca cgctgtcggc gcggtagccc tggtccaggg cgatgcccag ctgctcgagc tgggtcgaca tcggcggata gatggcctgg tcgctgccgc tgaccgtatg gcccagttcc cgcatatggt ggtggtggtg gtgagccatg g
Expected Protein Sequence
MAHHHHHHRE LGHTVSGSDQ AIYPPMSTQL EQLGIALDQG YRADSVPAGT DDVVIGNALS RGNAAVEAVL DAGQRYISGA QWLSEQVLPG RDTLAVAGTH GKTTTTTILT WLLESAGRSP GFLIGGVAED FGVSARLGQG REFVVEADEY DTAFFDKRSK FVHYRPLVAI LNNLEYDHAD IFPDVAAIQR QFHHLVRTVP ARGRLIVNGE DPHLAEVLAM GCWTPVERFG FDPSLEWHAE LLAADGSAFR VHHRGRAMGD VHWSLLGRHN VLNGLAALAA AHAVGVAPAD VIPALARFRS VKRRMEVIGR HDGITIYDDF AHHPTAIATT LEGLRAKVGD ARIVVAMEPR SNSMRLGAHA QALAPSLALA DEVVFLHRPE LAWDAAAVIA AVRGEAHAVP DTDALLAQLQ ASARSGDHVV FMSNGGFDGA PRRFLAALQA R
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgggaac tgggccatac ggtcagcggc agcgaccagg ccatctatcc gccgatgtcg acccagctcg agcagctggg catcgccctg gaccagggct accgcgccga cagcgtgccg gccggcaccg atgacgtggt gatcggcaac gccctctcgc gcggcaatgc cgccgttgag gccgtgctcg acgccggcca gcgctacatt tccggagcgc aatggctgtc cgaacaggta ctgccgggtc gcgacaccct ggccgtggca ggtacccatg gcaagaccac caccaccacc atcctcacct ggctgctgga aagcgccggt cgttcgcccg gtttcctgat cggcggcgtc gccgaggatt tcggcgtctc cgcccggctg ggccagggcc gcgagttcgt agtcgaggcc gacgagtacg acaccgcgtt cttcgacaag cgcagcaagt tcgtgcacta ccggccgctg gtggccatcc tcaacaacct tgaatacgac cacgccgaca tcttcccgga cgtggccgcg atccagcgcc agttccacca cctggtgcgc acggtgccgg cgcgtggccg actgatcgtc aacggcgagg acccgcatct ggccgaagtg ctggcgatgg gctgctggac cccggtcgag cgcttcggtt tcgacccgtc gctggaatgg catgccgagc tgctggccgc cgatggcagc gccttccgcg tacaccaccg cgggcgggca atgggcgatg tgcactggtc gttgctgggc cgccacaacg tgctcaacgg cctggctgcg ctggctgccg cacatgcggt gggcgtggcg ccggccgacg tgattcctgc gctggcgcgc ttccgcagcg tcaagcgccg catggaagtg atcggccgcc atgacggcat caccatctac gacgatttcg cccatcaccc gaccgcgatc gccaccacgc tggaaggctt gcgtgccaag gtgggtgacg cgcgcatcgt ggtcgcgatg gagccgcgca gcaattcgat gcggctgggc gctcacgcgc aggcgctggc cccgtcgctg gcgttggctg acgaggtggt gttcctgcat cgcccggaac tggcctggga tgcggccgcg gtgatcgccg cggtgcgcgg cgaagcgcac gcggtgcccg ataccgacgc gctgctggcg cagctgcagg ccagcgcgcg cagcggcgac cacgtggtgt tcatgtccaa tgggggcttc gacggcgcac cgcgtcgctt cctcgccgcg ctgcaggccc gatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat