StmaA.00133.a

Cysteinyl-tRNA synthetase (EC 6.1.1.16) (cysS)

CENTER ID: StmaA.00133.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00133.a.B1.GE43189 Full length(StmaA.00133.a) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00133.a.B1.PW38706 Full length(StmaA.00133.a) 1 465

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.3100

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSPSHSPMTL RLHNNLTRQL EPFTPLDPAC PTLYVCGPTV YNYVHIGNAR GPVVFGVLAD LLRRRFGGLR YARNITDVDD KINTAAREQG VPISTITDRF AAAYREDMAA LGVVPPDIEP EVTAHMPQII TMIEQLIANG HAYAAEGHVL FAVASFDGYG KLSRRDPEEM LAGARVDVAP YKRDPGDFVL WKPSSDDLPG WESPWGRGRP GWHIECSAMA AAHLGETIDI HAGGVDLQFP HHENEIAQSE CAHGGRIFAR YWLHNGMLNF GGAKMSKSLG NIERVHDLVR QHPPEALRLA LLSAHYRQPL DWSDGLIEQS VRTLDRLYGT LRELASIEAS AVIPASVEAT LDDDLNTPQA LAEVARIAAD ARRATDPAEQ ARLKGELLGA GLALGLLQAD PAQWFGTAAG DGGDDARIQG LIDERAAAKK ARDFARSDAI RDQLAAEGIV LEDTPQGVRW SRKRG
NT Sequence
TTGTCCCCCA GCCACAGCCC CATGACCCTG CGCCTGCACA ACAACCTGAC TCGCCAGCTC GAACCGTTCA CCCCGCTCGA TCCGGCCTGT CCGACCCTGT ATGTGTGCGG CCCCACGGTC TACAACTACG TGCACATCGG CAACGCCCGT GGCCCGGTGG TGTTCGGGGT GCTGGCCGAT CTGCTGCGGC GCCGTTTCGG TGGCCTGCGC TACGCGCGCA ACATCACCGA CGTGGACGAC AAGATCAACA CCGCTGCGCG CGAGCAGGGG GTGCCGATCA GCACCATCAC CGACAGGTTC GCCGCCGCCT ACCGTGAAGA CATGGCCGCG CTGGGCGTGG TGCCACCGGA CATCGAGCCG GAAGTGACTG CGCACATGCC GCAGATCATC ACCATGATCG AGCAGCTGAT CGCCAATGGT CATGCCTATG CCGCCGAAGG CCATGTGCTG TTCGCGGTGG CCAGCTTCGA CGGCTACGGC AAGCTCTCGC GACGCGATCC GGAAGAGATG CTGGCCGGCG CCCGCGTCGA TGTCGCCCCC TACAAGCGTG ATCCGGGCGA TTTCGTGCTG TGGAAGCCGT CCAGCGACGA CCTGCCCGGC TGGGAATCGC CCTGGGGCCG CGGCCGCCCG GGCTGGCACA TCGAATGCTC GGCGATGGCG GCCGCCCATC TGGGCGAGAC CATCGACATC CATGCCGGCG GTGTCGACCT GCAGTTCCCG CACCACGAGA ACGAGATCGC GCAGAGCGAA TGCGCGCATG GCGGCAGGAT CTTTGCCCGC TACTGGCTGC ACAACGGCAT GCTCAACTTC GGCGGCGCCA AGATGAGCAA GTCGCTGGGC AACATCGAGC GCGTACACGA CCTGGTGCGC CAGCATCCGC CGGAAGCGCT GCGTCTGGCC CTGCTGTCGG CCCATTACCG GCAGCCGCTG GACTGGTCCG ATGGCCTGAT CGAGCAGTCG GTGCGCACCC TGGACCGCCT GTACGGCACG CTGCGCGAGC TGGCGTCGAT CGAAGCCAGC GCGGTGATCC CGGCCAGCGT CGAGGCGACC CTGGATGACG ACCTGAACAC CCCGCAGGCG CTGGCCGAAG TGGCCCGCAT CGCCGCCGAT GCACGCCGCG CCACCGACCC GGCCGAACAG GCACGGCTGA AGGGCGAGCT GCTCGGCGCC GGCCTGGCGC TGGGCCTGCT GCAGGCCGAT CCGGCGCAGT GGTTCGGTAC CGCTGCGGGC GACGGCGGCG ACGATGCCCG CATCCAGGGC CTGATCGACG AGCGTGCTGC GGCCAAGAAA GCGCGTGATT TCGCCCGTTC CGATGCCATC CGCGACCAGC TGGCCGCCGA AGGCATCGTG CTGGAAGACA CCCCGCAGGG CGTGCGCTGG TCGCGCAAGC GCGGC
Details for StmaA.00133.a.B1.GE43189
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML SPSHSPMTLR LHNNLTRQLE PFTPLDPACP TLYVCGPTVY NYVHIGNARG PVVFGVLADL LRRRFGGLRY ARNITDVDDK INTAAREQGV PISTITDRFA AAYREDMAAL GVVPPDIEPE VTAHMPQIIT MIEQLIANGH AYAAEGHVLF AVASFDGYGK LSRRDPEEML AGARVDVAPY KRDPGDFVLW KPSSDDLPGW ESPWGRGRPG WHIECSAMAA AHLGETIDIH AGGVDLQFPH HENEIAQSEC AHGGRIFARY WLHNGMLNFG GAKMSKSLGN IERVHDLVRQ HPPEALRLAL LSAHYRQPLD WSDGLIEQSV RTLDRLYGTL RELASIEASA VIPASVEATL DDDLNTPQAL AEVARIAADA RRATDPAEQA RLKGELLGAG LALGLLQADP AQWFGTAAGD GGDDARIQGL IDERAAAKKA RDFARSDAIR DQLAAEGIVL EDTPQG
Validated NT Sequence
cnccctgcgg ggtgtcttcc agcacgatgc cttcggcggc cagctggtcg cggatggcat cggaacgggc gaaatcacgc gctttcttgg ccgcagcacg ctcgtcgatc aggccctgga tgcgggcatc gtcgccgccg tcgcccgcag cggtaccgaa ccactgcgcc ggatcggcct gcagcaggcc cagcgccagg ccggcgccga gcagctcgcc cttcagccgt gcctgttcgg ccgggtcggt ggcgcggcgt gcatcggcgg cgatgcgggc cacttcggcc agcgcctgcg gggtgttcag gtcgtcatcc agggtcgcct cgacgctggc cgggatcacc gcgctggctt cgatcgacgc cagctcgcgc agcgtgccgt acaggcggtc cagggtgcgc accgactgct cgatcaggcc atcggaccag tccagcggct gccggtaatg ggccgacagc agggccagac gcagcgcttc cggcggatgc tggcgcacca ggtcgtgtac gcgctcgatg ttgcccagcg acttgctcat cttggcgccg ccgaagttga gcatgccgtt gtgcagccag tagcgggcaa agatcctgcc gccatgcgcg cattcgctct gcgcgatctc gttctcgtgg tgcgggaact gcaggtcgac accgccggca tggatgtcga tggtctcgcc cagatgggcg gccgccatcg ccgagcattc gatgtgccag cccgggcggc cgcggcccca gggcgattcc cagccgggca ggtcgtcgct ggacggcttc cacagcacga aatcgcccgg atcacgcttg tagggggcga catcgacgcg ggcgccggcc agcatctctt ccggatcgcg tcgcgagagc ttgccgtagc cgtcgaagct ggccaccgcg aacagcacat ggccttcggc ggcataggca tgaccattgg cgatcagctg ctcgatcatg gtgatgatct gcggcatgtg cgcagtcact tccggctcga tgtccggtgg caccacgccc agcgcggcca tgtcttcacg gtaggcggcg gcgaacctgt cggtgatggt gctgatcggc accccctgct cgcgcgcagc ggtgttgatc ttgtcgtcca cgtcggtgat gttgcgcgcg tagcgcaggc caccgaaacg gcgccgcagc agatcggcca gcaccccgaa caccaccggg ccacgggcgt tgccgatgtg cacgtagttg tagaccgtgg ggccgcacac atacagggtc ggacaggccg gatcgagcgg ggtgaacggt tcgagctggc gagtcaggtt gttgtgcagg cgcagggtca tggggctgtg gctgggggac aacatatggt ggtggtggtg gtgagccatg gta
Expected Protein Sequence
MAHHHHHHMS PSHSPMTLRL HNNLTRQLEP FTPLDPACPT LYVCGPTVYN YVHIGNARGP VVFGVLADLL RRRFGGLRYA RNITDVDDKI NTAAREQGVP ISTITDRFAA AYREDMAALG VVPPDIEPEV TAHMPQIITM IEQLIANGHA YAAEGHVLFA VASFDGYGKL SRRDPEEMLA GARVDVAPYK RDPGDFVLWK PSSDDLPGWE SPWGRGRPGW HIECSAMAAA HLGETIDIHA GGVDLQFPHH ENEIAQSECA HGGRIFARYW LHNGMLNFGG AKMSKSLGNI ERVHDLVRQH PPEALRLALL SAHYRQPLDW SDGLIEQSVR TLDRLYGTLR ELASIEASAV IPASVEATLD DDLNTPQALA EVARIAADAR RATDPAEQAR LKGELLGAGL ALGLLQADPA QWFGTAAGDG GDDARIQGLI DERAAAKKAR DFARSDAIRD QLAAEGIVLE DTPQGVRWSR KRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtccc ccagccacag ccccatgacc ctgcgcctgc acaacaacct gactcgccag ctcgaaccgt tcaccccgct cgatccggcc tgtccgaccc tgtatgtgtg cggccccacg gtctacaact acgtgcacat cggcaacgcc cgtggcccgg tggtgttcgg ggtgctggcc gatctgctgc ggcgccgttt cggtggcctg cgctacgcgc gcaacatcac cgacgtggac gacaagatca acaccgctgc gcgcgagcag ggggtgccga tcagcaccat caccgacagg ttcgccgccg cctaccgtga agacatggcc gcgctgggcg tggtgccacc ggacatcgag ccggaagtga ctgcgcacat gccgcagatc atcaccatga tcgagcagct gatcgccaat ggtcatgcct atgccgccga aggccatgtg ctgttcgcgg tggccagctt cgacggctac ggcaagctct cgcgacgcga tccggaagag atgctggccg gcgcccgcgt cgatgtcgcc ccctacaagc gtgatccggg cgatttcgtg ctgtggaagc cgtccagcga cgacctgccc ggctgggaat cgccctgggg ccgcggccgc ccgggctggc acatcgaatg ctcggcgatg gcggccgccc atctgggcga gaccatcgac atccatgccg gcggtgtcga cctgcagttc ccgcaccacg agaacgagat cgcgcagagc gaatgcgcgc atggcggcag gatctttgcc cgctactggc tgcacaacgg catgctcaac ttcggcggcg ccaagatgag caagtcgctg ggcaacatcg agcgcgtaca cgacctggtg cgccagcatc cgccggaagc gctgcgtctg gccctgctgt cggcccatta ccggcagccg ctggactggt ccgatggcct gatcgagcag tcggtgcgca ccctggaccg cctgtacggc acgctgcgcg agctggcgtc gatcgaagcc agcgcggtga tcccggccag cgtcgaggcg accctggatg acgacctgaa caccccgcag gcgctggccg aagtggcccg catcgccgcc gatgcacgcc gcgccaccga cccggccgaa caggcacggc tgaagggcga gctgctcggc gccggcctgg cgctgggcct gctgcaggcc gatccggcgc agtggttcgg taccgctgcg ggcgacggcg gcgacgatgc ccgcatccag ggcctgatcg acgagcgtgc tgcggccaag aaagcgcgtg atttcgcccg ttccgatgcc atccgcgacc agctggccgc cgaaggcatc gtgctggaag acaccccgca gggcgtgcgc tggtcgcgca agcgcggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for StmaA.00133.a.B1.PW38706
PURIFICATION DATe: 9/20/2019
CONCENTRATION: 30.5mg/ml
OBSERVED MW: 52kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 17
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML SPSHSPMTLR LHNNLTRQLE PFTPLDPACP TLYVCGPTVY NYVHIGNARG PVVFGVLADL LRRRFGGLRY ARNITDVDDK INTAAREQGV PISTITDRFA AAYREDMAAL GVVPPDIEPE VTAHMPQIIT MIEQLIANGH AYAAEGHVLF AVASFDGYGK LSRRDPEEML AGARVDVAPY KRDPGDFVLW KPSSDDLPGW ESPWGRGRPG WHIECSAMAA AHLGETIDIH AGGVDLQFPH HENEIAQSEC AHGGRIFARY WLHNGMLNFG GAKMSKSLGN IERVHDLVRQ HPPEALRLAL LSAHYRQPLD WSDGLIEQSV RTLDRLYGTL RELASIEASA VIPASVEATL DDDLNTPQAL AEVARIAADA RRATDPAEQA RLKGELLGAG LALGLLQADP AQWFGTAAGD GGDDARIQGL IDERAAAKKA RDFARSDAIR DQLAAEGIVL EDTPQG
Validated NT Sequence
cnccctgcgg ggtgtcttcc agcacgatgc cttcggcggc cagctggtcg cggatggcat cggaacgggc gaaatcacgc gctttcttgg ccgcagcacg ctcgtcgatc aggccctgga tgcgggcatc gtcgccgccg tcgcccgcag cggtaccgaa ccactgcgcc ggatcggcct gcagcaggcc cagcgccagg ccggcgccga gcagctcgcc cttcagccgt gcctgttcgg ccgggtcggt ggcgcggcgt gcatcggcgg cgatgcgggc cacttcggcc agcgcctgcg gggtgttcag gtcgtcatcc agggtcgcct cgacgctggc cgggatcacc gcgctggctt cgatcgacgc cagctcgcgc agcgtgccgt acaggcggtc cagggtgcgc accgactgct cgatcaggcc atcggaccag tccagcggct gccggtaatg ggccgacagc agggccagac gcagcgcttc cggcggatgc tggcgcacca ggtcgtgtac gcgctcgatg ttgcccagcg acttgctcat cttggcgccg ccgaagttga gcatgccgtt gtgcagccag tagcgggcaa agatcctgcc gccatgcgcg cattcgctct gcgcgatctc gttctcgtgg tgcgggaact gcaggtcgac accgccggca tggatgtcga tggtctcgcc cagatgggcg gccgccatcg ccgagcattc gatgtgccag cccgggcggc cgcggcccca gggcgattcc cagccgggca ggtcgtcgct ggacggcttc cacagcacga aatcgcccgg atcacgcttg tagggggcga catcgacgcg ggcgccggcc agcatctctt ccggatcgcg tcgcgagagc ttgccgtagc cgtcgaagct ggccaccgcg aacagcacat ggccttcggc ggcataggca tgaccattgg cgatcagctg ctcgatcatg gtgatgatct gcggcatgtg cgcagtcact tccggctcga tgtccggtgg caccacgccc agcgcggcca tgtcttcacg gtaggcggcg gcgaacctgt cggtgatggt gctgatcggc accccctgct cgcgcgcagc ggtgttgatc ttgtcgtcca cgtcggtgat gttgcgcgcg tagcgcaggc caccgaaacg gcgccgcagc agatcggcca gcaccccgaa caccaccggg ccacgggcgt tgccgatgtg cacgtagttg tagaccgtgg ggccgcacac atacagggtc ggacaggccg gatcgagcgg ggtgaacggt tcgagctggc gagtcaggtt gttgtgcagg cgcagggtca tggggctgtg gctgggggac aacatatggt ggtggtggtg gtgagccatg gta
Expressed Protein Sequence
MAHHHHHHMS PSHSPMTLRL HNNLTRQLEP FTPLDPACPT LYVCGPTVYN YVHIGNARGP VVFGVLADLL RRRFGGLRYA RNITDVDDKI NTAAREQGVP ISTITDRFAA AYREDMAALG VVPPDIEPEV TAHMPQIITM IEQLIANGHA YAAEGHVLFA VASFDGYGKL SRRDPEEMLA GARVDVAPYK RDPGDFVLWK PSSDDLPGWE SPWGRGRPGW HIECSAMAAA HLGETIDIHA GGVDLQFPHH ENEIAQSECA HGGRIFARYW LHNGMLNFGG AKMSKSLGNI ERVHDLVRQH PPEALRLALL SAHYRQPLDW SDGLIEQSVR TLDRLYGTLR ELASIEASAV IPASVEATLD DDLNTPQALA EVARIAADAR RATDPAEQAR LKGELLGAGL ALGLLQADPA QWFGTAAGDG GDDARIQGLI DERAAAKKAR DFARSDAIRD QLAAEGIVLE DTPQGVRWSR KRG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATTTGTCCC CCAGCCACAG CCCCATGACC CTGCGCCTGC ACAACAACCT GACTCGCCAG CTCGAACCGT TCACCCCGCT CGATCCGGCC TGTCCGACCC TGTATGTGTG CGGCCCCACG GTCTACAACT ACGTGCACAT CGGCAACGCC CGTGGCCCGG TGGTGTTCGG GGTGCTGGCC GATCTGCTGC GGCGCCGTTT CGGTGGCCTG CGCTACGCGC GCAACATCAC CGACGTGGAC GACAAGATCA ACACCGCTGC GCGCGAGCAG GGGGTGCCGA TCAGCACCAT CACCGACAGG TTCGCCGCCG CCTACCGTGA AGACATGGCC GCGCTGGGCG TGGTGCCACC GGACATCGAG CCGGAAGTGA CTGCGCACAT GCCGCAGATC ATCACCATGA TCGAGCAGCT GATCGCCAAT GGTCATGCCT ATGCCGCCGA AGGCCATGTG CTGTTCGCGG TGGCCAGCTT CGACGGCTAC GGCAAGCTCT CGCGACGCGA TCCGGAAGAG ATGCTGGCCG GCGCCCGCGT CGATGTCGCC CCCTACAAGC GTGATCCGGG CGATTTCGTG CTGTGGAAGC CGTCCAGCGA CGACCTGCCC GGCTGGGAAT CGCCCTGGGG CCGCGGCCGC CCGGGCTGGC ACATCGAATG CTCGGCGATG GCGGCCGCCC ATCTGGGCGA GACCATCGAC ATCCATGCCG GCGGTGTCGA CCTGCAGTTC CCGCACCACG AGAACGAGAT CGCGCAGAGC GAATGCGCGC ATGGCGGCAG GATCTTTGCC CGCTACTGGC TGCACAACGG CATGCTCAAC TTCGGCGGCG CCAAGATGAG CAAGTCGCTG GGCAACATCG AGCGCGTACA CGACCTGGTG CGCCAGCATC CGCCGGAAGC GCTGCGTCTG GCCCTGCTGT CGGCCCATTA CCGGCAGCCG CTGGACTGGT CCGATGGCCT GATCGAGCAG TCGGTGCGCA CCCTGGACCG CCTGTACGGC ACGCTGCGCG AGCTGGCGTC GATCGAAGCC AGCGCGGTGA TCCCGGCCAG CGTCGAGGCG ACCCTGGATG ACGACCTGAA CACCCCGCAG GCGCTGGCCG AAGTGGCCCG CATCGCCGCC GATGCACGCC GCGCCACCGA CCCGGCCGAA CAGGCACGGC TGAAGGGCGA GCTGCTCGGC GCCGGCCTGG CGCTGGGCCT GCTGCAGGCC GATCCGGCGC AGTGGTTCGG TACCGCTGCG GGCGACGGCG GCGACGATGC CCGCATCCAG GGCCTGATCG ACGAGCGTGC TGCGGCCAAG AAAGCGCGTG ATTTCGCCCG TTCCGATGCC ATCCGCGACC AGCTGGCCGC CGAAGGCATC GTGCTGGAAG ACACCCCGCA GGGCGTGCGC TGGTCGCGCA AGCGCGGCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT