StmaA.00111.a

glutamine synthetase family protein

CENTER ID: StmaA.00111.a
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00111.a.B1.GE43187 Full length(StmaA.00111.a) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.1516

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSRTRPRKT ATPPAPQESS LLRWLKERRI TEVECLVPDI TGNARGKIIP ADKFSHDYGT RLPEGIFATT VTGEFPDDYY ELTSPSDSDM MLRPDPDTVR MVPWAADATA QVIHDCYTKN GEPHELAPRN VLRRVLAAYA ELGLRPVVAP ELEFFLVQKN TDPDFPLLPP AGRSGRPETA RQSYSIDAVN EFDPILDLMY DYADAMKLDV DTLIHESGAA QLEVNFTHAD AMDLADQVFL FKRTMREAAM RHGVYATFLA KPMENEPGSA MHIHQSLVRV SDGSNVFAGD TDAEGEFSPV FGHYLGGLQK YAPQAMAFFA PNVNSYRRLV FGEVSPSNVH WGFDNRTCGL RVPLDTPENM RVESRFAGSD ANPYLAMAAT LACGLLGIRE RLAPDAPVTG SAKELGYNLP RSLGEALDGL EQCSELQALL GERFCRAYIS VKRKEYETFF RVISSWEREF LLLNV
NT Sequence
ATGAGTTCCC GAACGCGCCC GCGCAAGACG GCTACGCCCC CCGCACCGCA GGAAAGCAGC CTGCTGCGCT GGCTGAAGGA GCGGCGCATC ACCGAGGTCG AATGCCTGGT GCCGGACATC ACCGGCAACG CGCGCGGCAA GATCATTCCC GCCGACAAGT TCTCGCATGA TTACGGTACG CGGCTGCCCG AAGGCATTTT CGCCACCACC GTCACCGGCG AGTTCCCTGA TGACTACTAC GAGCTGACCT CGCCGTCGGA CTCGGACATG ATGCTGCGTC CTGATCCGGA CACGGTGCGC ATGGTGCCGT GGGCGGCCGA CGCCACCGCG CAGGTCATCC ACGACTGTTA CACCAAGAAC GGTGAGCCGC ACGAGCTGGC GCCACGCAAC GTACTGCGCC GCGTGCTGGC GGCCTACGCC GAGCTGGGCC TGCGCCCGGT GGTGGCGCCG GAGCTTGAGT TCTTCCTGGT GCAGAAGAAC ACCGACCCGG ACTTCCCGCT GCTGCCACCG GCCGGCCGCT CCGGGCGCCC GGAAACCGCG CGGCAGTCGT ACTCGATCGA TGCGGTCAAC GAATTCGACC CGATCCTCGA CCTGATGTAC GACTACGCCG ATGCGATGAA GCTGGATGTG GACACGCTGA TCCACGAATC CGGTGCGGCG CAGCTGGAGG TCAACTTCAC CCATGCCGAT GCGATGGACC TGGCCGACCA GGTGTTCCTG TTCAAGCGCA CCATGCGCGA GGCGGCGATG CGCCACGGCG TGTATGCCAC CTTCCTGGCC AAGCCGATGG AGAACGAGCC GGGCAGTGCC ATGCACATCC ACCAGAGCCT GGTGCGGGTG AGCGACGGCA GCAATGTGTT CGCTGGCGAT ACCGATGCCG AAGGCGAGTT CAGCCCGGTG TTCGGCCACT ATCTGGGCGG CCTGCAGAAG TACGCGCCGC AGGCGATGGC GTTCTTCGCG CCGAACGTGA ATTCCTACCG CCGGCTGGTG TTCGGCGAAG TCTCGCCGAG CAACGTGCAC TGGGGCTTCG ACAACCGCAC CTGTGGCCTG CGCGTGCCGC TGGACACGCC GGAAAACATG CGCGTGGAGA GTCGTTTCGC CGGTTCCGAC GCCAACCCCT ACCTGGCGAT GGCGGCGACC CTGGCCTGCG GCCTGCTGGG CATCCGCGAG CGGTTGGCAC CGGATGCGCC GGTGACCGGC AGCGCCAAGG AACTGGGCTA CAACCTGCCG CGCTCGCTGG GCGAAGCGCT GGATGGGCTG GAGCAGTGCA GCGAACTGCA GGCCCTGCTG GGCGAGCGCT TCTGCCGGGC CTACATCTCG GTGAAGCGCA AGGAATACGA GACCTTTTTC CGTGTCATCA GCTCGTGGGA GCGCGAGTTC CTGCTGCTGA ACGTC
Details for StmaA.00111.a.B1.GE43187
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS SRTRPRKTAT PPAPQESSLL RWLKERRITE VECLVPDITG NARGKIIPAD KFSHDYGTRL PEGIFATTVT GEFPDDYYEL TSPSDSDMML RPDPDTVRMV PWAADATAQV IHDCYTKNGE PHELAPRNVL RRVLAAYAEL GLRPVVAPEL EFFLVQKNTD PDFPLLPPAG RSGRPETARQ SYSIDAVNEF DPILDLMYDY ADAMKLDVDT LIHESGAAQL EVNFTHADAM DLADQVFLFK RTMREAAMRH GVYATFLAKP MENEPGSAMH IHQSLVRVSD GSNVFAGDTD AEGEFSPVFG HYLGGLQKYA PQAMAFFAPN VNSYRRLVFG EVSPSNVHWG FDNRTCGLRV PLDTPENMRV ESRFAGSDAN PYLAMAATLA CGLLGIRERL APDAPVTGSA KELGYNLPRS LGEALDGLEQ CSELQALLGE RFCRAYISVK RKEYETFFRV ISSWEREFLL LNV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt cccgaacgcg cccgcgcaag acggctacgc cccccgcacc gcaggaaagc agcctgctgc gctggctgaa ggagcggcgc atcaccgagg tcgaatgcct ggtgccggac atcaccggca acgcgcgcgg caagatcatt cccgccgaca agttctcgca tgattacggt acgcggctgc ccgaaggcat tttcgccacc accgtcaccg gcgagttccc tgatgactac tacgagctga cctcgccgtc ggactcggac atgatgctgc gtcctgatcc ggacacggtg cgcatggtgc cgtgggcggc cgacgccacc gcgcaggtca tccacgactg ttacaccaag aacggtgagc cgcacgagct ggcgccacgc aacgtactgc gccgcgtgct ggcggcctac gccgagctgg gcctgcgccc ggtggtggcg ccggagcttg agttcttcct ggtgcagaag aacaccgacc cggacttccc gctgctgcca ccggccggcc gctccgggcg cccggaaacc gcgcggcagt cgtactcgat cgatgcggtc aacgaattcg acccgatcct cgacctgatg tacgactacg ccgatgcgat gaagctggat gtggacacgc tgatccacga atccggtgcg gcgcagctgg aggtcaactt cacccatgcc gatgcgatgg acctggccga ccaggtgttc ctgttcaagc gcaccatgcg cgaggcggcg atgcgccacg gcgtgtatgc caccttcctg gccaagccga tggagaacga gccgggcagt gccatgcaca tccaccagag cctggtgcgg gtgagcgacg gcagcaatgt gttcgctggc gataccgatg ccgaaggcga gttcagcccg gtgttcggcc actatctggg cggcctgcag aagtacgcgc cgcaggcgat ggcgttcttc gcgccgaacg tgaattccta ccgccggctg gtgttcggcg aagtctcgcc gagcaacgtg cactggggct tcgacaaccg cacctgtggc ctgcgcgtgc cgctggacac gccggaaaac atgcgcgtgg agagtcgttt cgccggttcc gacgccaacc cctacctggc gatggcggcg accctggcct gcggcctgct gggcatccgc gagcggttgg caccggatgc gccggtgacc ggcagcgcca aggaactggg ctacaacctg ccgcgctcgc tgggcgaagc gctggatggg ctggagcagt gcagcgaact gcaggccctg ctgggcgagc gcttctgccg ggcctacatc tcggtgaagc gcaaggaata cgagaccttt ttccgtgtca tcagctcgtg ggagcgcgag ttcctgctgc tgaacgtctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat