StmaA.00096.a

8-amino-7-oxononanoate synthase (EC 2.3.1.47) (bioF)

CENTER ID:	StmaA.00096.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00096.a.B1.GE43186	Full length(StmaA.00096.a)		1	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00096.a.B1.PW38699	Full length(StmaA.00096.a)		1	407

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|522373.3.peg.4204

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARPDLTARL HAQRALRDAQ GRRRPRRTVT RRDGVRLEVD GQWLTGFCSN DYLGLAQQFS VVNALQDAAA REGAGAGASH LVCGHHALHE ALEREVAEWL GYPRALLFGS GFAANLAVQQ ALLSEENDVC VQDKLNHASL LDATRLAGAR LRRYPHLDAE GAMRQLKHAP DGAAMLATDG VFSMDGDIAP LRALSLVARL QQALFYVDDA HGVGVVGDGR GAVAAAGLGV DDVPLQLVTL GKALGGSGAL VLGRDDLIEH LAETARPYIY TTAVPPAMAA AALEAVRLAR RDNWRRSKLT DLIALFRSEA RRHGLDLMAS DTPIQPLLCG DDHTAVAMST ALEQAGWLVG AIRPPTVPEG KARLRVTLSA LHTPEQVRGL VEAIASARER VALAVREAAP PPLPVFA

NT Sequence

ATGGCCCGCC CCGACCTGAC CGCCCGCCTC CACGCCCAGC GCGCTCTGCG CGATGCCCAA GGCCGTCGCC GCCCGCGACG CACGGTCACC CGTCGCGATG GCGTGCGCCT GGAAGTGGAC GGCCAGTGGC TGACCGGCTT CTGCAGCAAC GACTACCTCG GCCTGGCCCA GCAGTTCAGC GTGGTCAACG CGCTGCAGGA CGCCGCCGCA CGCGAAGGCG CTGGCGCAGG TGCCTCGCAC CTGGTCTGCG GCCATCATGC CCTGCATGAG GCGCTGGAAC GCGAGGTGGC CGAATGGCTT GGCTATCCGC GTGCGCTGCT GTTCGGCAGT GGCTTCGCCG CCAACCTGGC GGTGCAGCAG GCGCTGCTGA GCGAAGAGAA CGATGTCTGC GTACAGGACA AGCTCAACCA CGCCAGCCTG CTCGATGCCA CCCGGTTGGC CGGTGCACGC CTGCGCCGTT ATCCGCATCT GGATGCGGAA GGCGCAATGC GTCAGCTCAA GCATGCGCCC GATGGCGCGG CAATGCTGGC CACCGATGGT GTTTTCAGCA TGGATGGCGA CATCGCACCG CTGCGTGCAT TGTCGCTTGT GGCACGCCTG CAGCAGGCGC TGTTCTATGT GGATGATGCG CACGGTGTAG GCGTGGTCGG TGATGGCCGT GGTGCCGTGG CCGCCGCCGG ACTGGGCGTG GACGATGTGC CGCTGCAGCT GGTCACGCTG GGCAAGGCAC TGGGCGGTTC CGGTGCACTG GTGCTGGGCC GCGACGACCT GATCGAGCAT CTGGCCGAGA CCGCACGCCC GTACATCTAC ACCACCGCCG TGCCACCGGC GATGGCCGCC GCTGCGCTTG AAGCGGTGCG CCTGGCGCGC AGGGACAACT GGCGCCGTTC CAAACTGACC GACCTGATCG CGTTGTTCCG CAGCGAAGCA CGCCGCCATG GGCTGGACCT GATGGCTTCG GACACGCCGA TCCAACCGCT GCTTTGCGGC GATGACCACA CCGCCGTGGC CATGTCCACG GCGCTGGAAC AGGCCGGTTG GCTGGTCGGT GCGATCCGCC CGCCGACCGT GCCGGAAGGC AAGGCGCGCC TGCGGGTGAC ACTGTCTGCG CTGCACACGC CGGAACAGGT GCGGGGGCTG GTCGAGGCCA TCGCCAGCGC GCGCGAACGC GTGGCCCTGG CCGTGCGTGA AGCCGCGCCA CCGCCGCTGC CTGTCTTTGC C

Details for StmaA.00096.a.B1.GE43186

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/24/2019
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	78
PERCENT COVERAGE:	51

Validated AA Sequence

MPCMXXLERE XAEXLGYPRA LLFGSGFAAN LAVQQALLXX ENDVCVXDKL NHASLLDATR LXGARLRRYP HLDAEGAMRQ LKHAPDGAAM LATDGVFSMD GDIAPLRALS LVARLQQALF YVDDAHGVGV VGDGRGAVAA AGLGVDDVPL QLVTLGKALG GSGALVXXPR RPDRASXPRP HARTSTPPXC HRRWPPXRLK RCAWRAGTTG AVPN

Validated NT Sequence

tnnngcangn nnnnnnnnnn nngtnnnnnn gcgcngnnna tggcctccac cnnnccccgc acctgttccg gcgtgtgcag cncnnncagt gtcacccgca ggcgcgcctt gccttccggc acgntngncg ggcggatcgc accgaccnnn nnaccggcct gttccagcgc cnnnnncatg gccacggcgg tgtggtcatc gccgcaaagc agcggttgga tcggcgtgtc cgaanncatc angtccagcc catggcggcg tgcttcgctg cggaacaacg cgatcaggtc ggtcagtttg gaacggcgcc agttgtccct gcgcgccagg cgcaccgctt caagcgcngc ggcggccatc gccggtggca nngcggtggt gtagatgtac gggcgtgcgg tctcggcnnn gatgctcgat caggtcgtcg cggccnnnnn accagtgcac cggaaccgcc cagtgccttg cccagcgtga ccagctgcag cggcacatcg tccacgccca gtccggcggc ggccacggca ccacggccat caccgaccac gcctacaccg tgcgcatcat ccacatagaa cagcgcctgc tgcaggcgtg ccacaagcga caatgcacgc agcggtgcga tgtcgccatc catgctgaaa acaccatcgg tggccagcat tgccgcgcca tcgggcgcat gcttgagctg acgcattgcg ccttccgcat ccagatgcgg ataacggcgc aggcgtgcac cggncaaccg ggtggcatcg agcaggctgg cgtggttgag cttgtccnnt acgcagacat cgttctctnn gcncagcagc gcctgctgca ccgccaggtt ggcggcgaag ccactgccga acagcagcgc acgcggatag ccaagncatt cggccnnctc gcgttccagc gnnnncatgc agggcatnnn nggncgcagn nnaggtgcga ggcnnnngnn ncagcgcnnn nnnnnncgnn nnnntcctgc agcgcgnnnn ccacnnngaa ctgnngggnn aggccgaggt agnngnnnct gcagaagccg gtcancnnnt ggnnnnnnnn nnnnaggcgc annccnnnnn nacggnnnnn ngtgcgtcgc gggcggcgan nnnnttgggc atcgcgcaga gcnnnctggg cgtggaggcg ggcggtcagg tcgggannnn ncatatggtg gtggtggtgg tgagccnnng t

Expected Protein Sequence

MAHHHHHHMA RPDLTARLHA QRALRDAQGR RRPRRTVTRR DGVRLEVDGQ WLTGFCSNDY LGLAQQFSVV NALQDAAARE GAGAGASHLV CGHHALHEAL EREVAEWLGY PRALLFGSGF AANLAVQQAL LSEENDVCVQ DKLNHASLLD ATRLAGARLR RYPHLDAEGA MRQLKHAPDG AAMLATDGVF SMDGDIAPLR ALSLVARLQQ ALFYVDDAHG VGVVGDGRGA VAAAGLGVDD VPLQLVTLGK ALGGSGALVL GRDDLIEHLA ETARPYIYTT AVPPAMAAAA LEAVRLARRD NWRRSKLTDL IALFRSEARR HGLDLMASDT PIQPLLCGDD HTAVAMSTAL EQAGWLVGAI RPPTVPEGKA RLRVTLSALH TPEQVRGLVE AIASARERVA LAVREAAPPP LPVFA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggccc gccccgacct gaccgcccgc ctccacgccc agcgcgctct gcgcgatgcc caaggccgtc gccgcccgcg acgcacggtc acccgtcgcg atggcgtgcg cctggaagtg gacggccagt ggctgaccgg cttctgcagc aacgactacc tcggcctggc ccagcagttc agcgtggtca acgcgctgca ggacgccgcc gcacgcgaag gcgctggcgc aggtgcctcg cacctggtct gcggccatca tgccctgcat gaggcgctgg aacgcgaggt ggccgaatgg cttggctatc cgcgtgcgct gctgttcggc agtggcttcg ccgccaacct ggcggtgcag caggcgctgc tgagcgaaga gaacgatgtc tgcgtacagg acaagctcaa ccacgccagc ctgctcgatg ccacccggtt ggccggtgca cgcctgcgcc gttatccgca tctggatgcg gaaggcgcaa tgcgtcagct caagcatgcg cccgatggcg cggcaatgct ggccaccgat ggtgttttca gcatggatgg cgacatcgca ccgctgcgtg cattgtcgct tgtggcacgc ctgcagcagg cgctgttcta tgtggatgat gcgcacggtg taggcgtggt cggtgatggc cgtggtgccg tggccgccgc cggactgggc gtggacgatg tgccgctgca gctggtcacg ctgggcaagg cactgggcgg ttccggtgca ctggtgctgg gccgcgacga cctgatcgag catctggccg agaccgcacg cccgtacatc tacaccaccg ccgtgccacc ggcgatggcc gccgctgcgc ttgaagcggt gcgcctggcg cgcagggaca actggcgccg ttccaaactg accgacctga tcgcgttgtt ccgcagcgaa gcacgccgcc atgggctgga cctgatggct tcggacacgc cgatccaacc gctgctttgc ggcgatgacc acaccgccgt ggccatgtcc acggcgctgg aacaggccgg ttggctggtc ggtgcgatcc gcccgccgac cgtgccggaa ggcaaggcgc gcctgcgggt gacactgtct gcgctgcaca cgccggaaca ggtgcggggg ctggtcgagg ccatcgccag cgcgcgcgaa cgcgtggccc tggccgtgcg tgaagccgcg ccaccgccgc tgcctgtctt tgcctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for StmaA.00096.a.B1.PW38699

PURIFICATION DATe:	9/6/2019
CONCENTRATION:	13mg/ml
OBSERVED MW:	44kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	14
VIAL VOLUME:	110µl
PERCENT IDENTITY:	78
PERCENT COVERAGE:	51

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCC GCCCCGACCT GACCGCCCGC CTCCACGCCC AGCGCGCTCT GCGCGATGCC CAAGGCCGTC GCCGCCCGCG ACGCACGGTC ACCCGTCGCG ATGGCGTGCG CCTGGAAGTG GACGGCCAGT GGCTGACCGG CTTCTGCAGC AACGACTACC TCGGCCTGGC CCAGCAGTTC AGCGTGGTCA ACGCGCTGCA GGACGCCGCC GCACGCGAAG GCGCTGGCGC AGGTGCCTCG CACCTGGTCT GCGGCCATCA TGCCCTGCAT GAGGCGCTGG AACGCGAGGT GGCCGAATGG CTTGGCTATC CGCGTGCGCT GCTGTTCGGC AGTGGCTTCG CCGCCAACCT GGCGGTGCAG CAGGCGCTGC TGAGCGAAGA GAACGATGTC TGCGTACAGG ACAAGCTCAA CCACGCCAGC CTGCTCGATG CCACCCGGTT GGCCGGTGCA CGCCTGCGCC GTTATCCGCA TCTGGATGCG GAAGGCGCAA TGCGTCAGCT CAAGCATGCG CCCGATGGCG CGGCAATGCT GGCCACCGAT GGTGTTTTCA GCATGGATGG CGACATCGCA CCGCTGCGTG CATTGTCGCT TGTGGCACGC CTGCAGCAGG CGCTGTTCTA TGTGGATGAT GCGCACGGTG TAGGCGTGGT CGGTGATGGC CGTGGTGCCG TGGCCGCCGC CGGACTGGGC GTGGACGATG TGCCGCTGCA GCTGGTCACG CTGGGCAAGG CACTGGGCGG TTCCGGTGCA CTGGTGCTGG GCCGCGACGA CCTGATCGAG CATCTGGCCG AGACCGCACG CCCGTACATC TACACCACCG CCGTGCCACC GGCGATGGCC GCCGCTGCGC TTGAAGCGGT GCGCCTGGCG CGCAGGGACA ACTGGCGCCG TTCCAAACTG ACCGACCTGA TCGCGTTGTT CCGCAGCGAA GCACGCCGCC ATGGGCTGGA CCTGATGGCT TCGGACACGC CGATCCAACC GCTGCTTTGC GGCGATGACC ACACCGCCGT GGCCATGTCC ACGGCGCTGG AACAGGCCGG TTGGCTGGTC GGTGCGATCC GCCCGCCGAC CGTGCCGGAA GGCAAGGCGC GCCTGCGGGT GACACTGTCT GCGCTGCACA CGCCGGAACA GGTGCGGGGG CTGGTCGAGG CCATCGCCAG CGCGCGCGAA CGCGTGGCCC TGGCCGTGCG TGAAGCCGCG CCACCGCCGC TGCCTGTCTT TGCCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT