StmaA.00085.b

dTDP-glucose 4,6-dehydratase (EC 4.2.1.46) (rfbB)

CENTER ID: StmaA.00085.b
ORGANISM: Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: Emerging
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00085.b.B1.GE43185 Full length(StmaA.00085.b) 1 351
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
StmaA.00085.b.B1.PW38704 Full length(StmaA.00085.b) 1 351

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|522373.3.peg.616

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPTWLVTGGA GFIGGNFVLE AVARGVKVIN LDALTYAGNL KTLSSLDGNP NHVFVQGDIG DSALVTRLLA EHQPDAVLNF AAESHVDRSI DGPGAFIQTN VVGTLGLLEA VRDYWKALPT EQGAAFRFLH VSTDEVYGTL GETGKFSETT PYAPNSPYSA SKAASDHLVR AFHHTYGLPV LTTNCSNNYG PYHFPEKLIP LVIAKALAGE PLPVYGDGKQ VRDWLFVSDH CEAIRTVLAK GQVGETYNVG GNSEKQNIEV VQAICALLDQ RRPRADGQPR SSQITYVTDR PGHDRRYAID ASKLKNDLGW EPAYTFEQGI TFTVDWYLDN QEWVNGVLDG SYRLQRIGTA A
NT Sequence
GTGCCCACAT GGCTTGTCAC CGGCGGCGCC GGATTCATTG GCGGTAACTT CGTTCTCGAG GCCGTTGCGC GCGGCGTCAA GGTCATCAAC CTCGATGCGC TGACCTACGC CGGCAACCTG AAGACCCTGT CCAGCCTGGA CGGCAACCCG AATCACGTGT TCGTGCAGGG TGACATCGGC GACAGCGCCC TGGTCACCCG CCTGCTGGCT GAGCACCAGC CCGATGCGGT GCTGAACTTC GCGGCCGAGA GCCACGTCGA CCGCTCCATC GATGGCCCGG GCGCCTTCAT CCAGACCAAC GTGGTGGGCA CCCTGGGCCT GCTGGAAGCC GTGCGCGACT ACTGGAAGGC GCTGCCGACC GAACAGGGCG CGGCGTTCCG CTTCCTGCAT GTGTCCACCG ACGAGGTGTA CGGCACCCTG GGCGAGACCG GCAAGTTCAG CGAAACCACG CCGTATGCCC CGAATTCGCC GTACTCGGCG TCCAAGGCCG CCTCGGACCA CCTGGTGCGC GCCTTCCACC ACACCTACGG GTTGCCGGTA CTGACCACCA ACTGCTCCAA CAACTATGGC CCGTACCATT TCCCGGAGAA GCTGATCCCG CTGGTGATCG CCAAGGCGCT GGCCGGCGAG CCGCTGCCGG TGTACGGCGA TGGCAAGCAG GTGCGCGACT GGCTGTTCGT GTCCGACCAC TGCGAAGCGA TCCGCACCGT GCTGGCCAAG GGCCAGGTTG GCGAGACCTA CAACGTCGGC GGCAATTCGG AAAAGCAGAA CATCGAAGTG GTGCAGGCCA TCTGCGCACT GCTGGACCAG CGCCGCCCGC GCGCGGATGG CCAGCCGCGC AGCAGCCAGA TCACCTACGT CACCGACCGC CCCGGCCATG ACCGCCGTTA CGCGATCGAT GCCTCCAAGC TGAAGAACGA CCTGGGCTGG GAACCGGCCT ACACCTTCGA GCAGGGCATC ACCTTTACCG TTGACTGGTA CCTGGACAAC CAGGAATGGG TCAACGGCGT GCTCGACGGC AGCTACCGTC TGCAGCGCAT CGGCACCGCG GCC
Details for StmaA.00085.b.B1.GE43185
HARVESTED ON: 5/17/2019
SEQUENCED ON: 5/24/2019
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMV PTWLVTGGAG FIGGNFVLEA VARGVKVINL DALTYAGNLK TLSSLDGNPN HVFVQGDIGD SALVTRLLAE HQPDAVLNFA AESHVDRSID GPGAFIQTNV VGTLGLLEAV RDYWKALPTE QGAAFRFLHV STDEVYGTLG ETGKFSETTP YAPNSPYSAS KAASDHLVRA FHHTYGLPVL TTNCSNNYGP YHFPEKLIPL VIAKALAGEP LPVYGDGKQV RDWLFVSDHC EAIRTVLAKG QVGETYNVGG NSEKQNIEVV QAICALLDQR RPRADGQPRS SQITYVTDRP GHDRRYAIDA SKLKNDLGWE PAYTFEQGIT FTVDWYLDNQ EWVNGVLDGS
Validated NT Sequence
gtagctgccg tcgagcacgc cgttgaccca ttcctggttg tccaggtacc agtcaacggt aaaggtgatg ccctgctcga aggtgtaggc cggttcccag cccaggtcgt tcttcagctt ggaggcatcg atcgcgtaac ggcggtcatg gccggggcgg tcggtgacgt aggtgatctg gctgctgcgc ggctggccat ccgcgcgcgg gcggcgctgg tccagcagtg cgcagatggc ctgcaccact tcgatgttct gcttttccga attgccgccg acgttgtagg tctcgccaac ctggcccttg gccagcacgg tgcggatcgc ttcgcagtgg tcggacacga acagccagtc gcgcacctgc ttgccatcgc cgtacaccgg cagcggctcg ccggccagcg ccttggcgat caccagcggg atcagcttct ccgggaaatg gtacgggcca tagttgttgg agcagttggt ggtcagtacc ggcaacccgt aggtgtggtg gaaggcgcgc accaggtggt ccgaggcggc cttggacgcc gagtacggcg aattcggggc atacggcgtg gtttcgctga acttgccggt ctcgcccagg gtgccgtaca cctcgtcggt ggacacatgc aggaagcgga acgccgcgcc ctgttcggtc ggcagcgcct tccagtagtc gcgcacggct tccagcaggc ccagggtgcc caccacgttg gtctggatga aggcgcccgg gccatcgatg gagcggtcga cgtggctctc ggccgcgaag ttcagcaccg catcgggctg gtgctcagcc agcaggcggg tgaccagggc gctgtcgccg atgtcaccct gcacgaacac gtgattcggg ttgccgtcca ggctggacag ggtcttcagg ttgccggcgt aggtcagcgc atcgaggttg atgaccttga cgccgcgcgc aacggcctcg agaacgaagt taccgccaat gaatccggcg ccgccggtga caagccatgt gggcaccata tggtggtggt ggtggtgagc catgg
Expected Protein Sequence
MAHHHHHHMP TWLVTGGAGF IGGNFVLEAV ARGVKVINLD ALTYAGNLKT LSSLDGNPNH VFVQGDIGDS ALVTRLLAEH QPDAVLNFAA ESHVDRSIDG PGAFIQTNVV GTLGLLEAVR DYWKALPTEQ GAAFRFLHVS TDEVYGTLGE TGKFSETTPY APNSPYSASK AASDHLVRAF HHTYGLPVLT TNCSNNYGPY HFPEKLIPLV IAKALAGEPL PVYGDGKQVR DWLFVSDHCE AIRTVLAKGQ VGETYNVGGN SEKQNIEVVQ AICALLDQRR PRADGQPRSS QITYVTDRPG HDRRYAIDAS KLKNDLGWEP AYTFEQGITF TVDWYLDNQE WVNGVLDGSY RLQRIGTAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgccca catggcttgt caccggcggc gccggattca ttggcggtaa cttcgttctc gaggccgttg cgcgcggcgt caaggtcatc aacctcgatg cgctgaccta cgccggcaac ctgaagaccc tgtccagcct ggacggcaac ccgaatcacg tgttcgtgca gggtgacatc ggcgacagcg ccctggtcac ccgcctgctg gctgagcacc agcccgatgc ggtgctgaac ttcgcggccg agagccacgt cgaccgctcc atcgatggcc cgggcgcctt catccagacc aacgtggtgg gcaccctggg cctgctggaa gccgtgcgcg actactggaa ggcgctgccg accgaacagg gcgcggcgtt ccgcttcctg catgtgtcca ccgacgaggt gtacggcacc ctgggcgaga ccggcaagtt cagcgaaacc acgccgtatg ccccgaattc gccgtactcg gcgtccaagg ccgcctcgga ccacctggtg cgcgccttcc accacaccta cgggttgccg gtactgacca ccaactgctc caacaactat ggcccgtacc atttcccgga gaagctgatc ccgctggtga tcgccaaggc gctggccggc gagccgctgc cggtgtacgg cgatggcaag caggtgcgcg actggctgtt cgtgtccgac cactgcgaag cgatccgcac cgtgctggcc aagggccagg ttggcgagac ctacaacgtc ggcggcaatt cggaaaagca gaacatcgaa gtggtgcagg ccatctgcgc actgctggac cagcgccgcc cgcgcgcgga tggccagccg cgcagcagcc agatcaccta cgtcaccgac cgccccggcc atgaccgccg ttacgcgatc gatgcctcca agctgaagaa cgacctgggc tgggaaccgg cctacacctt cgagcagggc atcaccttta ccgttgactg gtacctggac aaccaggaat gggtcaacgg cgtgctcgac ggcagctacc gtctgcagcg catcggcacc gcggcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for StmaA.00085.b.B1.PW38704
PURIFICATION DATe: 9/18/2019
CONCENTRATION: 106mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV PTWLVTGGAG FIGGNFVLEA VARGVKVINL DALTYAGNLK TLSSLDGNPN HVFVQGDIGD SALVTRLLAE HQPDAVLNFA AESHVDRSID GPGAFIQTNV VGTLGLLEAV RDYWKALPTE QGAAFRFLHV STDEVYGTLG ETGKFSETTP YAPNSPYSAS KAASDHLVRA FHHTYGLPVL TTNCSNNYGP YHFPEKLIPL VIAKALAGEP LPVYGDGKQV RDWLFVSDHC EAIRTVLAKG QVGETYNVGG NSEKQNIEVV QAICALLDQR RPRADGQPRS SQITYVTDRP GHDRRYAIDA SKLKNDLGWE PAYTFEQGIT FTVDWYLDNQ EWVNGVLDGS
Validated NT Sequence
gtagctgccg tcgagcacgc cgttgaccca ttcctggttg tccaggtacc agtcaacggt aaaggtgatg ccctgctcga aggtgtaggc cggttcccag cccaggtcgt tcttcagctt ggaggcatcg atcgcgtaac ggcggtcatg gccggggcgg tcggtgacgt aggtgatctg gctgctgcgc ggctggccat ccgcgcgcgg gcggcgctgg tccagcagtg cgcagatggc ctgcaccact tcgatgttct gcttttccga attgccgccg acgttgtagg tctcgccaac ctggcccttg gccagcacgg tgcggatcgc ttcgcagtgg tcggacacga acagccagtc gcgcacctgc ttgccatcgc cgtacaccgg cagcggctcg ccggccagcg ccttggcgat caccagcggg atcagcttct ccgggaaatg gtacgggcca tagttgttgg agcagttggt ggtcagtacc ggcaacccgt aggtgtggtg gaaggcgcgc accaggtggt ccgaggcggc cttggacgcc gagtacggcg aattcggggc atacggcgtg gtttcgctga acttgccggt ctcgcccagg gtgccgtaca cctcgtcggt ggacacatgc aggaagcgga acgccgcgcc ctgttcggtc ggcagcgcct tccagtagtc gcgcacggct tccagcaggc ccagggtgcc caccacgttg gtctggatga aggcgcccgg gccatcgatg gagcggtcga cgtggctctc ggccgcgaag ttcagcaccg catcgggctg gtgctcagcc agcaggcggg tgaccagggc gctgtcgccg atgtcaccct gcacgaacac gtgattcggg ttgccgtcca ggctggacag ggtcttcagg ttgccggcgt aggtcagcgc atcgaggttg atgaccttga cgccgcgcgc aacggcctcg agaacgaagt taccgccaat gaatccggcg ccgccggtga caagccatgt gggcaccata tggtggtggt ggtggtgagc catgg
Expressed Protein Sequence
MAHHHHHHMP TWLVTGGAGF IGGNFVLEAV ARGVKVINLD ALTYAGNLKT LSSLDGNPNH VFVQGDIGDS ALVTRLLAEH QPDAVLNFAA ESHVDRSIDG PGAFIQTNVV GTLGLLEAVR DYWKALPTEQ GAAFRFLHVS TDEVYGTLGE TGKFSETTPY APNSPYSASK AASDHLVRAF HHTYGLPVLT TNCSNNYGPY HFPEKLIPLV IAKALAGEPL PVYGDGKQVR DWLFVSDHCE AIRTVLAKGQ VGETYNVGGN SEKQNIEVVQ AICALLDQRR PRADGQPRSS QITYVTDRPG HDRRYAIDAS KLKNDLGWEP AYTFEQGITF TVDWYLDNQE WVNGVLDGSY RLQRIGTAA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGCCCA CATGGCTTGT CACCGGCGGC GCCGGATTCA TTGGCGGTAA CTTCGTTCTC GAGGCCGTTG CGCGCGGCGT CAAGGTCATC AACCTCGATG CGCTGACCTA CGCCGGCAAC CTGAAGACCC TGTCCAGCCT GGACGGCAAC CCGAATCACG TGTTCGTGCA GGGTGACATC GGCGACAGCG CCCTGGTCAC CCGCCTGCTG GCTGAGCACC AGCCCGATGC GGTGCTGAAC TTCGCGGCCG AGAGCCACGT CGACCGCTCC ATCGATGGCC CGGGCGCCTT CATCCAGACC AACGTGGTGG GCACCCTGGG CCTGCTGGAA GCCGTGCGCG ACTACTGGAA GGCGCTGCCG ACCGAACAGG GCGCGGCGTT CCGCTTCCTG CATGTGTCCA CCGACGAGGT GTACGGCACC CTGGGCGAGA CCGGCAAGTT CAGCGAAACC ACGCCGTATG CCCCGAATTC GCCGTACTCG GCGTCCAAGG CCGCCTCGGA CCACCTGGTG CGCGCCTTCC ACCACACCTA CGGGTTGCCG GTACTGACCA CCAACTGCTC CAACAACTAT GGCCCGTACC ATTTCCCGGA GAAGCTGATC CCGCTGGTGA TCGCCAAGGC GCTGGCCGGC GAGCCGCTGC CGGTGTACGG CGATGGCAAG CAGGTGCGCG ACTGGCTGTT CGTGTCCGAC CACTGCGAAG CGATCCGCAC CGTGCTGGCC AAGGGCCAGG TTGGCGAGAC CTACAACGTC GGCGGCAATT CGGAAAAGCA GAACATCGAA GTGGTGCAGG CCATCTGCGC ACTGCTGGAC CAGCGCCGCC CGCGCGCGGA TGGCCAGCCG CGCAGCAGCC AGATCACCTA CGTCACCGAC CGCCCCGGCC ATGACCGCCG TTACGCGATC GATGCCTCCA AGCTGAAGAA CGACCTGGGC TGGGAACCGG CCTACACCTT CGAGCAGGGC ATCACCTTTA CCGTTGACTG GTACCTGGAC AACCAGGAAT GGGTCAACGG CGTGCTCGAC GGCAGCTACC GTCTGCAGCG CATCGGCACC GCGGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T