StmaA.00085.a
UDP-glucose 4-epimerase (EC 5.1.3.2) (galE)
| CENTER ID: | StmaA.00085.a |
| ORGANISM: | Stenotrophomonas maltophilia K279a |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | Emerging |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
non-BEI |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00085.a.B1.GE43184 | Full length(StmaA.00085.a) | 1 | 346 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| StmaA.00085.a.B1.PW38702 | Full length(StmaA.00085.a) | 1 | 346 |
Structures
7KN1
DEPOSITED: 11/3/2020
DETERMINATION: XRay
CLONE: StmaA.00085.a.B1.GE43184
PROTEIN: StmaA.00085.a.B1.PW38702
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|522373.3.peg.3201 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVLVTGGAG YIGSHACVEL QQQGHGVVIV DSLCNSDASV VERIGRITGT APVFVQADIR DRPRMAALMQ EHAIDAVLHF AALKSVGESQ KIPLQYFDSN ISGSIALLGA MQDAGVQLLV FSSSATVYGN QDHCPVAETA STCAMTPYGR TKLVVEQLLA DLAATGQDLH IATLRYFNPV GAHASALIGE LPHGTPSNLM PYIAQVAAGL LPEVQVFGDD YPTHDGTGVR DYIHVQDVAS AHVLALQFLR DQRRSITLNL GTGQGHSVLE LIQAFELTTG VRVPFRIVPR RDGDIAVSFA DASLALRELG WKARHDLTDM CRDTWKWQRA MSRAAQGAVR EHLPAR
NT Sequence
ATGAACGTAC TGGTGACCGG CGGTGCTGGC TACATCGGCT CGCATGCATG CGTGGAGCTG CAGCAGCAGG GGCACGGCGT GGTCATCGTG GACTCGCTGT GCAACAGCGA CGCCAGCGTG GTCGAGCGCA TCGGACGGAT CACCGGCACG GCACCGGTGT TTGTGCAGGC TGACATCCGG GACCGGCCGC GCATGGCCGC CTTGATGCAG GAGCACGCGA TCGATGCCGT ACTGCACTTC GCAGCGCTCA AGTCCGTGGG CGAATCCCAG AAGATTCCAC TGCAGTATTT CGACAGCAAC ATCAGCGGTT CGATTGCCCT GCTCGGTGCC ATGCAGGATG CAGGCGTGCA GTTGCTGGTC TTCAGCTCTT CGGCAACGGT GTACGGCAAC CAGGACCACT GCCCGGTGGC CGAGACCGCT TCAACCTGTG CGATGACGCC CTATGGGCGC ACCAAGCTGG TGGTCGAGCA GCTGCTTGCC GACCTGGCGG CAACGGGCCA GGACCTGCAT ATCGCGACGC TGCGCTACTT CAACCCGGTC GGCGCCCACG CCAGCGCGCT GATCGGCGAG CTGCCGCATG GCACGCCGAG CAACCTGATG CCCTACATCG CGCAGGTCGC AGCCGGGCTG CTGCCGGAGG TCCAGGTCTT CGGGGACGAC TATCCGACCC ATGATGGTAC CGGCGTGCGC GACTACATCC ATGTGCAGGA CGTCGCCAGC GCCCACGTAC TGGCCCTGCA GTTCCTTCGC GACCAGCGGC GCAGCATCAC CCTCAACCTT GGCACCGGCC AGGGCCACAG CGTGCTGGAA CTGATCCAGG CCTTCGAGCT CACCACCGGC GTACGGGTTC CGTTCCGCAT CGTACCGCGG CGCGATGGCG ACATCGCGGT CAGCTTCGCC GATGCCTCGC TGGCGCTGCG CGAGCTGGGC TGGAAGGCGC GTCACGATCT CACCGACATG TGCCGGGACA CCTGGAAGTG GCAGCGGGCG ATGTCACGCG CCGCACAAGG TGCAGTGCGA GAGCACCTGC CCGCGCGC
Details for StmaA.00085.a.B1.GE43184
| HARVESTED ON: | 5/17/2019 |
| SEQUENCED ON: | 5/24/2019 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHMX XXVTGGAGYI GSHACVELQQ QGHGVVIVDS LCNSDASVVE RIGRITGTAP VFVQADIRDR PRMAALMQEH AIDAVLHFAA LKSVGESQKI PLQYFDSNIS GSIALLGAMQ DAGVQLLVFS SSATVYGNQD HCPVAETAST CAMTPYGRTK LVVEQLLADL AATGQDLHIA TLRYFNPVGA HASALIGELP HGTPSNLMPY IAQVAAGLLP EVQVFGDDYP THDGTGVRDY IHVQDVASAH VLALQFLRDQ RRSITLNLGT GQGHSVLELI QAFELTTGVR VPFRIVPRRD GDIAVSFADA SLALRELGWK ARHDLTDMCR DTWKWQRAMS RAA
Validated NT Sequence
ttgtgcggcg cgtgacatcg cccgctgcca cttccaggtg tcccggcaca tgtcggtgag atcgtgacgc gccttccagc ccagctcgcg cagcgccagc gaggcatcgg cgaagctgac cgcgatgtcg ccatcgcgcc gcggtacgat gcggaacgga acccgtacgc cggtggtgag ctcgaaggcc tggatcagtt ccagcacgct gtggccctgg ccggtgccaa ggttgagggt gatgctgcgc cgctggtcgc gaaggaactg cagggccagt acgtgggcgc tggcgacgtc ctgcacatgg atgtagtcgc gcacgccggt accatcatgg gtcggatagt cgtccccgaa gacctggacc tccggcagca gcccggctgc gacctgcgcg atgtagggca tcaggttgct cggcgtgcca tgcggcagct cgccgatcag cgcgctggcg tgggcgccga ccgggttgaa gtagcgcagc gtcgcgatat gcaggtcctg gcccgttgcc gccaggtcgg caagcagctg ctcgaccacc agcttggtgc gcccataggg cgtcatcgca caggttgaag cggtctcggc caccgggcag tggtcctggt tgccgtacac cgttgccgaa gagctgaaga ccagcaactg cacgcctgca tcctgcatgg caccgagcag ggcaatcgaa ccgctgatgt tgctgtcgaa atactgcagt ggaatcttct gggattcgcc cacggacttg agcgctgcga agtgcagtac ggcatcgatc gcgtgctcct gcatcaaggc ggccatgcgc ggccggtccc ggatgtcagc ctgcacaaac accggtgccg tgccggtgat ccgtccgatg cgctcgacca cgctggcgtc gctgttgcac agcgagtcca cgatgaccac gccgtgcccc tgctgctgca gctccacgca tgcatgcgag ccgatgtagc cagcaccgcc ggtcaccann nnnttcatat ggtggtggtg gtggtgagcc atgg
Expected Protein Sequence
MAHHHHHHMN VLVTGGAGYI GSHACVELQQ QGHGVVIVDS LCNSDASVVE RIGRITGTAP VFVQADIRDR PRMAALMQEH AIDAVLHFAA LKSVGESQKI PLQYFDSNIS GSIALLGAMQ DAGVQLLVFS SSATVYGNQD HCPVAETAST CAMTPYGRTK LVVEQLLADL AATGQDLHIA TLRYFNPVGA HASALIGELP HGTPSNLMPY IAQVAAGLLP EVQVFGDDYP THDGTGVRDY IHVQDVASAH VLALQFLRDQ RRSITLNLGT GQGHSVLELI QAFELTTGVR VPFRIVPRRD GDIAVSFADA SLALRELGWK ARHDLTDMCR DTWKWQRAMS RAAQGAVREH LPAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg tactggtgac cggcggtgct ggctacatcg gctcgcatgc atgcgtggag ctgcagcagc aggggcacgg cgtggtcatc gtggactcgc tgtgcaacag cgacgccagc gtggtcgagc gcatcggacg gatcaccggc acggcaccgg tgtttgtgca ggctgacatc cgggaccggc cgcgcatggc cgccttgatg caggagcacg cgatcgatgc cgtactgcac ttcgcagcgc tcaagtccgt gggcgaatcc cagaagattc cactgcagta tttcgacagc aacatcagcg gttcgattgc cctgctcggt gccatgcagg atgcaggcgt gcagttgctg gtcttcagct cttcggcaac ggtgtacggc aaccaggacc actgcccggt ggccgagacc gcttcaacct gtgcgatgac gccctatggg cgcaccaagc tggtggtcga gcagctgctt gccgacctgg cggcaacggg ccaggacctg catatcgcga cgctgcgcta cttcaacccg gtcggcgccc acgccagcgc gctgatcggc gagctgccgc atggcacgcc gagcaacctg atgccctaca tcgcgcaggt cgcagccggg ctgctgccgg aggtccaggt cttcggggac gactatccga cccatgatgg taccggcgtg cgcgactaca tccatgtgca ggacgtcgcc agcgcccacg tactggccct gcagttcctt cgcgaccagc ggcgcagcat caccctcaac cttggcaccg gccagggcca cagcgtgctg gaactgatcc aggccttcga gctcaccacc ggcgtacggg ttccgttccg catcgtaccg cggcgcgatg gcgacatcgc ggtcagcttc gccgatgcct cgctggcgct gcgcgagctg ggctggaagg cgcgtcacga tctcaccgac atgtgccggg acacctggaa gtggcagcgg gcgatgtcac gcgccgcaca aggtgcagtg cgagagcacc tgcccgcgcg ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for StmaA.00085.a.B1.PW38702
| PURIFICATION DATe: | 9/16/2019 |
| CONCENTRATION: | 48.5mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMX XXVTGGAGYI GSHACVELQQ QGHGVVIVDS LCNSDASVVE RIGRITGTAP VFVQADIRDR PRMAALMQEH AIDAVLHFAA LKSVGESQKI PLQYFDSNIS GSIALLGAMQ DAGVQLLVFS SSATVYGNQD HCPVAETAST CAMTPYGRTK LVVEQLLADL AATGQDLHIA TLRYFNPVGA HASALIGELP HGTPSNLMPY IAQVAAGLLP EVQVFGDDYP THDGTGVRDY IHVQDVASAH VLALQFLRDQ RRSITLNLGT GQGHSVLELI QAFELTTGVR VPFRIVPRRD GDIAVSFADA SLALRELGWK ARHDLTDMCR DTWKWQRAMS RAA
Validated NT Sequence
ttgtgcggcg cgtgacatcg cccgctgcca cttccaggtg tcccggcaca tgtcggtgag atcgtgacgc gccttccagc ccagctcgcg cagcgccagc gaggcatcgg cgaagctgac cgcgatgtcg ccatcgcgcc gcggtacgat gcggaacgga acccgtacgc cggtggtgag ctcgaaggcc tggatcagtt ccagcacgct gtggccctgg ccggtgccaa ggttgagggt gatgctgcgc cgctggtcgc gaaggaactg cagggccagt acgtgggcgc tggcgacgtc ctgcacatgg atgtagtcgc gcacgccggt accatcatgg gtcggatagt cgtccccgaa gacctggacc tccggcagca gcccggctgc gacctgcgcg atgtagggca tcaggttgct cggcgtgcca tgcggcagct cgccgatcag cgcgctggcg tgggcgccga ccgggttgaa gtagcgcagc gtcgcgatat gcaggtcctg gcccgttgcc gccaggtcgg caagcagctg ctcgaccacc agcttggtgc gcccataggg cgtcatcgca caggttgaag cggtctcggc caccgggcag tggtcctggt tgccgtacac cgttgccgaa gagctgaaga ccagcaactg cacgcctgca tcctgcatgg caccgagcag ggcaatcgaa ccgctgatgt tgctgtcgaa atactgcagt ggaatcttct gggattcgcc cacggacttg agcgctgcga agtgcagtac ggcatcgatc gcgtgctcct gcatcaaggc ggccatgcgc ggccggtccc ggatgtcagc ctgcacaaac accggtgccg tgccggtgat ccgtccgatg cgctcgacca cgctggcgtc gctgttgcac agcgagtcca cgatgaccac gccgtgcccc tgctgctgca gctccacgca tgcatgcgag ccgatgtagc cagcaccgcc ggtcaccann nnnttcatat ggtggtggtg gtggtgagcc atgg
Expressed Protein Sequence
MAHHHHHHMN VLVTGGAGYI GSHACVELQQ QGHGVVIVDS LCNSDASVVE RIGRITGTAP VFVQADIRDR PRMAALMQEH AIDAVLHFAA LKSVGESQKI PLQYFDSNIS GSIALLGAMQ DAGVQLLVFS SSATVYGNQD HCPVAETAST CAMTPYGRTK LVVEQLLADL AATGQDLHIA TLRYFNPVGA HASALIGELP HGTPSNLMPY IAQVAAGLLP EVQVFGDDYP THDGTGVRDY IHVQDVASAH VLALQFLRDQ RRSITLNLGT GQGHSVLELI QAFELTTGVR VPFRIVPRRD GDIAVSFADA SLALRELGWK ARHDLTDMCR DTWKWQRAMS RAAQGAVREH LPAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG TACTGGTGAC CGGCGGTGCT GGCTACATCG GCTCGCATGC ATGCGTGGAG CTGCAGCAGC AGGGGCACGG CGTGGTCATC GTGGACTCGC TGTGCAACAG CGACGCCAGC GTGGTCGAGC GCATCGGACG GATCACCGGC ACGGCACCGG TGTTTGTGCA GGCTGACATC CGGGACCGGC CGCGCATGGC CGCCTTGATG CAGGAGCACG CGATCGATGC CGTACTGCAC TTCGCAGCGC TCAAGTCCGT GGGCGAATCC CAGAAGATTC CACTGCAGTA TTTCGACAGC AACATCAGCG GTTCGATTGC CCTGCTCGGT GCCATGCAGG ATGCAGGCGT GCAGTTGCTG GTCTTCAGCT CTTCGGCAAC GGTGTACGGC AACCAGGACC ACTGCCCGGT GGCCGAGACC GCTTCAACCT GTGCGATGAC GCCCTATGGG CGCACCAAGC TGGTGGTCGA GCAGCTGCTT GCCGACCTGG CGGCAACGGG CCAGGACCTG CATATCGCGA CGCTGCGCTA CTTCAACCCG GTCGGCGCCC ACGCCAGCGC GCTGATCGGC GAGCTGCCGC ATGGCACGCC GAGCAACCTG ATGCCCTACA TCGCGCAGGT CGCAGCCGGG CTGCTGCCGG AGGTCCAGGT CTTCGGGGAC GACTATCCGA CCCATGATGG TACCGGCGTG CGCGACTACA TCCATGTGCA GGACGTCGCC AGCGCCCACG TACTGGCCCT GCAGTTCCTT CGCGACCAGC GGCGCAGCAT CACCCTCAAC CTTGGCACCG GCCAGGGCCA CAGCGTGCTG GAACTGATCC AGGCCTTCGA GCTCACCACC GGCGTACGGG TTCCGTTCCG CATCGTACCG CGGCGCGATG GCGACATCGC GGTCAGCTTC GCCGATGCCT CGCTGGCGCT GCGCGAGCTG GGCTGGAAGG CGCGTCACGA TCTCACCGAC ATGTGCCGGG ACACCTGGAA GTGGCAGCGG GCGATGTCAC GCGCCGCACA AGGTGCAGTG CGAGAGCACC TGCCCGCGCG CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT