SSGCID: Target Page

StmaA.00082.a

Porphobilinogen deaminase (EC 2.5.1.61) (hemC)

CENTER ID:	StmaA.00082.a
ORGANISM:	Stenotrophomonas maltophilia K279a
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	Emerging
SELECT AGENT:	False
NIH PRIORITY pathogens category:	non-BEI

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00082.a.B1.GE43183	Full length(StmaA.00082.a)		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
StmaA.00082.a.B1.PW38703	Full length(StmaA.00082.a)		1	303

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|522373.3.peg.3901

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

METVRIATRK SPLALWQSEH VADRLRQAHP GLHVELVPMS TRGDEVLDRS LAAIGGKGLF LKELELAMLR GEADCAVHSL KDVPMELDPP FALPAMLTRH DPADGFVSNL YASLDALPIG ARVGTSSLRR QAQLRALRPD LELLDLRGNV NTRLAKLDNG GYDAIVLAVA GLERLGLGER IVARLQPPQW LPAPAQGAVA VECDGGNARL MALFAPLDDA ATRACVEAER AMNRALHGSC HVPVAAIAQW QGDDLHLQGL VGSASDGRAV RAEAVGPAND PEGLGQRVAK LLLDDGAGEL LNV

NT Sequence

ATGGAAACCG TCCGCATCGC CACCCGCAAG AGCCCGCTCG CCCTCTGGCA GAGCGAACAC GTCGCCGACC GCCTGCGCCA GGCCCATCCC GGCTTGCATG TGGAACTGGT GCCGATGAGC ACCCGCGGCG ACGAAGTGCT CGACCGTTCG CTGGCCGCCA TCGGTGGCAA GGGCCTGTTC CTGAAGGAGC TGGAGCTGGC CATGCTGCGC GGTGAGGCCG ACTGCGCGGT GCACTCGCTG AAGGACGTGC CGATGGAACT GGACCCGCCG TTCGCGTTGC CGGCAATGCT GACCCGCCAC GATCCCGCCG ATGGCTTCGT CTCCAATCTG TACGCCTCGC TGGACGCGCT GCCGATCGGT GCGCGGGTCG GGACCTCGTC GCTGCGTCGC CAGGCGCAGC TGCGCGCGCT GCGACCGGAC CTGGAACTGC TCGACCTGCG CGGTAACGTC AACACCCGGC TGGCCAAACT CGACAACGGT GGCTACGACG CCATCGTGCT GGCCGTGGCC GGCCTGGAGC GTCTGGGTCT GGGCGAGCGC ATCGTCGCGC GCCTGCAACC GCCGCAGTGG TTGCCGGCCC CGGCGCAGGG CGCGGTGGCG GTGGAGTGCG ATGGTGGCAA TGCGCGGCTG ATGGCGTTGT TCGCCCCGCT TGATGACGCG GCCACGCGTG CCTGCGTGGA AGCCGAGCGG GCGATGAACC GTGCGTTGCA TGGCAGCTGC CACGTGCCGG TGGCGGCCAT AGCGCAGTGG CAGGGCGATG ACCTGCACCT GCAGGGCCTG GTTGGCAGCG CCAGCGACGG CCGTGCGGTG CGCGCCGAAG CCGTGGGCCC CGCCAATGAC CCGGAAGGCC TGGGCCAGCG CGTGGCGAAG CTGCTGCTGG ACGATGGCGC CGGCGAACTG CTGAACGTC

Details for StmaA.00082.a.B1.GE43183

HARVESTED ON:	5/17/2019
SEQUENCED ON:	5/25/2019
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHME XXRIATRKSP LALWQSEHVA DRLRQAHPGL HVELVPMSTR GDEVLDRSLA AIGGKGLFLK ELELAMLRGE ADCAVHSLKD VPMELDPPFA LPAMLTRHDP ADGFVSNLYA SLDALPIGAR VGTSSLRRQA QLRALRPDLE LLDLRGNVNT RLAKLDNGGY DAIVLAVAGL ERLGLGERIV ARLQPPQWLP APAQGAVAVE CDGGNARLMA LFAPLDDAAT RACVEAERAM NRALHGSCHV PVAAIAQWQG DDLHLQGLVG SASDGRAVRA EAVGPANDPE GXGQRVAKL

Validated NT Sequence

gcagcttcgc cacgcgctgg cccnnncctt ccgggtcatt ggcggggccc acggcttcgg cgcgcaccgc acggccgtcg ctggcgctgc caaccaggcc ctgcaggtgc aggtcatcgc cctgccactg cgctatggcc gccaccggca cgtggcagct gccatgcaac gcacggttca tcgcccgctc ggcttccacg caggcacgcg tggccgcgtc atcaagcggg gcgaacaacg ccatcagccg cgcattgcca ccatcgcact ccaccgccac cgcgccctgc gccggggccg gcaaccactg cggcggttgc aggcgcgcga cgatgcgctc gcccagaccc agacgctcca ggccggccac ggccagcacg atggcgtcgt agccaccgtt gtcgagtttg gccagccggg tgttgacgtt accgcgcagg tcgagcagtt ccaggtccgg tcgcagcgcg cgcagctgcg cctggcgacg cagcgacgag gtcccgaccc gcgcaccgat cggcagcgcg tccagcgagg cgtacagatt ggagacgaag ccatcggcgg gatcgtggcg ggtcagcatt gccggcaacg cgaacggcgg gtccagttcc atcggcacgt ccttcagcga gtgcaccgcg cagtcggcct caccgcgcag catggccagc tccagctcct tcaggaacag gcccttgcca ccgatggcgg ccagcgaacg gtcgagcact tcgtcgccgc gggtgctcat cggcaccagt tccacatgca agccgggatg ggcctggcgc aggcggtcgg cgacgtgttc gctctgccag agggcgagcg ggctcttgcg ggtggcgatg cggangnntt ccatatggtg gtggtggtgg tgagccatg

Expected Protein Sequence

MAHHHHHHME TVRIATRKSP LALWQSEHVA DRLRQAHPGL HVELVPMSTR GDEVLDRSLA AIGGKGLFLK ELELAMLRGE ADCAVHSLKD VPMELDPPFA LPAMLTRHDP ADGFVSNLYA SLDALPIGAR VGTSSLRRQA QLRALRPDLE LLDLRGNVNT RLAKLDNGGY DAIVLAVAGL ERLGLGERIV ARLQPPQWLP APAQGAVAVE CDGGNARLMA LFAPLDDAAT RACVEAERAM NRALHGSCHV PVAAIAQWQG DDLHLQGLVG SASDGRAVRA EAVGPANDPE GLGQRVAKLL LDDGAGELLN V

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaaa ccgtccgcat cgccacccgc aagagcccgc tcgccctctg gcagagcgaa cacgtcgccg accgcctgcg ccaggcccat cccggcttgc atgtggaact ggtgccgatg agcacccgcg gcgacgaagt gctcgaccgt tcgctggccg ccatcggtgg caagggcctg ttcctgaagg agctggagct ggccatgctg cgcggtgagg ccgactgcgc ggtgcactcg ctgaaggacg tgccgatgga actggacccg ccgttcgcgt tgccggcaat gctgacccgc cacgatcccg ccgatggctt cgtctccaat ctgtacgcct cgctggacgc gctgccgatc ggtgcgcggg tcgggacctc gtcgctgcgt cgccaggcgc agctgcgcgc gctgcgaccg gacctggaac tgctcgacct gcgcggtaac gtcaacaccc ggctggccaa actcgacaac ggtggctacg acgccatcgt gctggccgtg gccggcctgg agcgtctggg tctgggcgag cgcatcgtcg cgcgcctgca accgccgcag tggttgccgg ccccggcgca gggcgcggtg gcggtggagt gcgatggtgg caatgcgcgg ctgatggcgt tgttcgcccc gcttgatgac gcggccacgc gtgcctgcgt ggaagccgag cgggcgatga accgtgcgtt gcatggcagc tgccacgtgc cggtggcggc catagcgcag tggcagggcg atgacctgca cctgcagggc ctggttggca gcgccagcga cggccgtgcg gtgcgcgccg aagccgtggg ccccgccaat gacccggaag gcctgggcca gcgcgtggcg aagctgctgc tggacgatgg cgccggcgaa ctgctgaacg tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for StmaA.00082.a.B1.PW38703

PURIFICATION DATe:	9/17/2019
CONCENTRATION:	19.6mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	96

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAA CCGTCCGCAT CGCCACCCGC AAGAGCCCGC TCGCCCTCTG GCAGAGCGAA CACGTCGCCG ACCGCCTGCG CCAGGCCCAT CCCGGCTTGC ATGTGGAACT GGTGCCGATG AGCACCCGCG GCGACGAAGT GCTCGACCGT TCGCTGGCCG CCATCGGTGG CAAGGGCCTG TTCCTGAAGG AGCTGGAGCT GGCCATGCTG CGCGGTGAGG CCGACTGCGC GGTGCACTCG CTGAAGGACG TGCCGATGGA ACTGGACCCG CCGTTCGCGT TGCCGGCAAT GCTGACCCGC CACGATCCCG CCGATGGCTT CGTCTCCAAT CTGTACGCCT CGCTGGACGC GCTGCCGATC GGTGCGCGGG TCGGGACCTC GTCGCTGCGT CGCCAGGCGC AGCTGCGCGC GCTGCGACCG GACCTGGAAC TGCTCGACCT GCGCGGTAAC GTCAACACCC GGCTGGCCAA ACTCGACAAC GGTGGCTACG ACGCCATCGT GCTGGCCGTG GCCGGCCTGG AGCGTCTGGG TCTGGGCGAG CGCATCGTCG CGCGCCTGCA ACCGCCGCAG TGGTTGCCGG CCCCGGCGCA GGGCGCGGTG GCGGTGGAGT GCGATGGTGG CAATGCGCGG CTGATGGCGT TGTTCGCCCC GCTTGATGAC GCGGCCACGC GTGCCTGCGT GGAAGCCGAG CGGGCGATGA ACCGTGCGTT GCATGGCAGC TGCCACGTGC CGGTGGCGGC CATAGCGCAG TGGCAGGGCG ATGACCTGCA CCTGCAGGGC CTGGTTGGCA GCGCCAGCGA CGGCCGTGCG GTGCGCGCCG AAGCCGTGGG CCCCGCCAAT GACCCGGAAG GCCTGGGCCA GCGCGTGGCG AAGCTGCTGC TGGACGATGG CGCCGGCGAA CTGCTGAACG TCTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT