ScmaA.19677.a

Calpain (C02 family) (Sm-p80)

CENTER ID: ScmaA.19677.a
ORGANISM: Schistosoma mansoni
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 1
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ScmaA.19677.a.B4.GE42771 truncation 2 812 1205
ScmaA.19677.a.B7.GE42772 truncation 5 834 1205
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
ScmaA.19677.a.B4.PW38622 truncation 2 812 1205
ScmaA.19677.a.B7.PW38600 truncation 5 834 1205
ScmaA.19677.a.B7.PW38601 truncation 5 834 1205

External Resources

RESOURCE REFERENCE ID
UniProt: P27730

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRRKKQNSDS ETECNGVPLN YLDILKPTLT EKRVQFNLNI PCSITPKGYA KLHEKLTSPC IPHSKGKADS MADRVYRQIF LYCTNSLNGF THSSKSETTY ASPTAMRKIK ISQYEIISAK MVEAKELYQD PEFPANDASI GNLPHVSGRV EWKRPKDINP LAAFCTKSYS RFDVDQGALG DCWFASVMAT VTKYPSLMSN IIPPNQTFKG SGYTGAFVFN FWRFGEWVEV VVDDRIPVVK GTCNPVFIHS TDNSEFWSCL LEKAYAKLCG SYAHLTGGFQ SEAMEDFTGG ICTTLILNKK ERPPNLLKMM EHYTKTCCVM GADVGGDKNK RREELGLIGS HAYSVTGVGK VNYMGKDVYL VRCRNPWGER HEWKGPWSDN SPEWKDVKSK DKRALQYKPK EDGEFCSFQL TFILFISTSI SRRNVLFGLP LFRFPSPFQV RACLVMQFGD FLNVCPIHFQ QSFCTNPQYL FTIGTDNSGK VGKAHIIVGV MQEHRRFLYG GEYLAIGFSI FEVSELQNTR LTPEELLCRK PLLTSDYIPR REVTLEADLF PGTFILIPST YNSNQEARYI CRVFSTVKMK QLELDEENSY EGFPKKELAS LELNFKQLCN PVTEKINSRI LGTILDQSNF GEYMDGFTEF PSNLCSSLIS SASTNLTGQI DLEEYLYLLI DLKGWMLTFN KYDDRKGCVS WFKFRDLLKS SGYKVSNRVF SVLAHRHIDH KRGHISLESY LHCMANLKFA FDVTSYHPKS DKGILMFNKE DVYSPDENVS GRTNRPGKEV VDPRTGRIIK VKRETPDDYL NVLKPIKGPK RMEFNPYLPK TLTPKGYAKF KLMMNVASKQ YETLVKRLKT ERTLWEDPDF PANDKAIGNL PDFRERIEWK RPHEINPNAK FFAGGASRFD IEQGALGDCW LLAVVASISG YPQLFDQVVP KDQELKGPEY VGVVRFRFWR FGHWVEVLID DRLPVRQGRN TLVFMHSNDP TEFWSALLEK AYAKLNGCYA HLSGGSQSEA MEDLTGGICL SLELNQKERP SDLIDQLKIY AQRCCLMGCS IDSSVMEQKM DNGLIGSHAY SLTGVYPVNY RGRTQWLMRL RNPWGDSHEW KGAWCDGSPQ WREISEQEKK NINLSFTADG EFWMSYEDFV TCFSRVEVCH LGLESLEYNQ NFHGKRRLDE AIFSGQWQRN VNAGGCINNR TTYWTNPQFR ITVEDPDPDD DDNKCSVLIG LMQTDIRKKV GADFQPIGFM VYNAPDDLNT LLSRAQLLTR SPIAKSQFIN TREVTAQFRV PPGSYVVIPS TFDPNIEVNF ILRVFSQTSI TEQELDEDNT NQGLPDDVIE ALKLEDTLLD EDQEIEQKFL AIRDPKTNAI NAVKLGELLN NSTLQDIPNF QGFNKELCRS MVASVDNNLT GHVELNEFMD LWIQAKGWKH IFIKHDVDQS GYFSAYEFRE ALNDAGYHVS NRLINAIINR YQDPGTDKIS FEDFMLCMVR LKTAFETIEA HPKNIEGTSL FSAEDYLRFS VYI
NT Sequence
atgagacgta aaaagcagaa tagtgattca gaaactgagt gtaatggagt tccactgaat tatttggaca ttcttaaacc aacattaaca gagaaacgag tacaatttaa tttaaatata ccatgttcaa ttactccgaa gggttatgca aagctgcatg aaaaattaac ttcaccatgc atccctcatt ctaagggaaa agcggattcc atggctgatc gtgtatacag acaaatattt ctctattgta caaacagttt gaatggtttt actcattctt cgaaaagtga aacaacttat gcaagtccta ctgcgatgag gaaaataaaa attagtcagt atgaaatcat ttcagcgaaa atggtagaag caaaagaact ttatcaagat cctgagtttc ccgcaaacga tgcttcaatt ggaaacttgc cacatgtctc gggcagagta gagtggaaaa gacccaaaga tattaatcct cttgctgctt tttgtacaaa atcctattct cgttttgatg ttgatcaagg tgctcttggt gactgttggt tcgcttcggt tatggctaca gttacgaaat atccttcatt gatgagtaac attataccac caaatcaaac tttcaaaggt tcaggttata caggagcatt tgtgttcaat ttttggcgtt ttggagagtg ggtggaagtt gtagttgatg atcgaatacc agttgttaag ggaacatgta atccagtatt cattcattca acggataata gtgagttttg gtcatgccta ctggaaaaag catatgcaaa attatgcggt agctatgcac atctaactgg tggatttcaa agtgaagcaa tggaggactt tactggtgga atatgtacta ctcttatttt gaataaaaaa gaacgcccac caaacttatt aaaaatgatg gaacactaca caaaaacatg ttgtgtaatg ggtgctgatg ttggtggaga taaaaataaa agacgcgaag aactgggact gattggttct cacgcttata gtgtaactgg ggttgggaaa gttaattata tgggtaaaga tgtatattta gtacgttgtc gtaatccttg gggagaaaga catgaatgga aaggaccttg gtcagataat tcacctgaat ggaaagatgt taaatctaaa gacaaaagag cattacaata taaaccaaaa gaagatggag aattctgttc tttccagtta acattcatcc ttttcatatc tacttccatc tcccgacgta atgtgctctt tggccttcct ctctttcgct tcccctcacc attccaagtt agggcttgcc tcgtgatgca gtttggtgat ttcctcaatg tatgtcctat ccacttccaa caatcgttct gtacgaatcc acagtattta tttacaattg gaactgataa tagtggtaaa gttggtaaag cacatatcat cgtaggagtt atgcaagaac acagaagatt tttatatggc ggagaatatt tggctatagg tttttcaata tttgaagtat ctgaactaca aaatacacgt ctcacaccag aagaattatt atgccgtaaa ccattgttaa catcagacta tattccaaga cgtgaagtaa cattagaagc tgaccttttt cctggaacat ttatcttaat accatcaaca tataattcaa atcaagaagc acgatatatt tgtcgagtat tctctacagt aaaaatgaaa caattagaac tggatgagga gaattcctac gaaggctttc caaagaagga acttgcttcg ttggaattaa attttaagca gttgtgtaat ccagtcactg aaaaaatcaa ttctagaata ttaggtacaa ttctagatca aagtaatttt ggagaatata tggatggttt tactgaattt cccagtaact tgtgtagtag tttaatatct tcagcatcca caaacttgac gggtcaaatt gacttagaag agtacctata tctcttgatt gatcttaagg gatggatgct tacttttaat aaatatgatg atcggaaagg atgtgtgagt tggtttaaat tccgcgattt attgaaaagt tcaggttaca aagtcagtaa tcgagttttc agtgttttgg cacatcgaca tatagatcac aaacgaggtc atattagctt ggaaagctat ctgcattgta tggcaaacct taaatttgcc tttgatgtca cctcgtacca tccgaaaagc gataagggta ttctaatgtt taataaagaa gatgtttatt ctcctgatga aaatgtatct ggacgaacta accgtcctgg taaagaggta gtggatccac gtactggtag aataatcaaa gtgaaacgag aaactccaga tgattacttg aatgtattaa aacccatcaa aggtccaaag cgtatggaat ttaatccata tcttcctaaa acacttactc caaaaggata tgctaagttt aaactaatga tgaatgttgc aagtaaacaa tatgaaacgc tagttaaacg tttaaaaact gaacgaacac tctgggaaga tcctgacttt ccagctaatg ataaagcaat tgggaatctg ccagatttca gagagagaat tgaatggaaa agaccacatg aaattaatcc aaatgctaaa tttttcgctg gtggagcaag tcgtttcgat attgaacaag gagcacttgg tgactgctgg ttacttgcag tagtggcttc catatcagga tacccacagc tatttgatca agtggtgcct aaagatcaag aattgaaagg accagaatat gttggtgttg tacgttttcg gttttggcgt tttggtcact gggtggaagt gcttatagat gatcgtcttc cggttcgtca aggacgtaat acactcgttt ttatgcattc taatgatccc acggaattct ggtcagctct gttggaaaaa gcttatgcaa aattgaatgg ttgttatgct catctgagtg gaggatcaca aagtgaagct atggaagatt taactggtgg aatttgttta tctcttgaac tgaaccaaaa agaacgtcca agtgatctta tagatcagct taaaatttat gctcaacgtt gttgccttat gggttgtagt attgattcat cagttatgga acaaaaaatg gataatggac ttataggtag tcacgcatac agtttaacag gtgtatatcc cgtcaactac cgaggtcgca ctcaatggtt aatgcgttta cgtaatccat ggggtgacag tcatgaatgg aaaggtgcat ggtgtgatgg ttcacctcaa tggcgtgaaa taagtgaaca agaaaagaaa aatattaact tgagcttcac tgcagatgga gaattttgga tgtcatatga agattttgtt acttgttttt cacgagttga agtatgtcat ttaggtcttg aaagtttaga gtataatcaa aatttccatg ggaaaagaag attagacgaa gctatatttt ctggtcaatg gcaacgtaac gttaatgctg gtggttgtat taataatcgc actacctatt ggacgaatcc acaatttcgc atcacagttg aagatccaga tcctgatgac gatgataata agtgttcagt actcattggt ttaatgcaaa cagatatcag gaagaaagtc ggagcagatt ttcaacctat aggttttatg gtttataatg cacctgatga tttaaacact ttattatcac gtgcacaact tttaactaga tctccaatag ctaaatcaca atttattaat acacgtgaag ttactgcaca atttcgtgta ccaccaggat catatgttgt tattccgagt acattcgatc caaatattga agtaaatttt atattacgtg tattttcaca aacatctatt acagaacaag aacttgatga agacaatact aaccaaggtc taccagatga tgtgattgaa gctttgaaat tagaagatac tttgttagat gaagatcaag aaattgaaca gaaattttta gctatccgtg atccaaaaac taatgctata aacgctgtta aattgggtga acttttaaat aatagtacat tacaagatat ccctaacttc caaggattta ataaagaatt atgtcgtagt atggttgcat ctgtagataa taacttaaca ggtcatgttg aattaaacga atttatggat ctttggatac aagctaaagg atggaaacat atatttataa aacatgatgt tgatcaaagt ggttatttca gtgcatatga atttcgtgaa gcactgaatg atgcaggtta tcatgtgagt aatcgattaa tcaatgctat tataaatcga tatcaagatc ctggcacaga taaaataagt tttgaagatt ttatgttgtg catggtacga cttaaaactg cctttgaaac aattgaagca catccgaaaa atattgaagg cacatcactt ttcagtgctg aagactatct acgattttca gtttatatct ga
Details for ScmaA.19677.a.B4.GE42771
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHME FNPYLPKTLT PKGYAKFKLM MNVASKQYET LVKRLKTERT LWEDPDFPAN DKAIGNLPDF RERIEWKRPH EINPNAKFFA GGASRFDIEQ GALGDCWLLA VVASISGYPQ LFDQVVPKDQ ELKGPEYVGV VRFRFWRFGH WVEVLIDDRL PVRQGRNTLV FMHSNDPTEF WSALLEKAYA KLNGCYAHLS GGSQSEAMED LTGGICLSLE LNQKERPSDL IDQLKIYAQR CCLMGCSIDS SVMEQKMDNG LIGSHAYSLT GVYPVNYRGR TQWLMRLRNP WGDSHEWKGA WCDGSPQWRE ISEQEKKNIN LSFTADGEFW MSYEDFVTCF SRVEVCHLGL ESLEYNQNFH GKRRLDEAIF SGQWQRNVNA GGCINNRTTY WTNPQFRITV ED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat ttaatccata tcttcctaaa acacttactc caaaaggata tgctaagttt aaactaatga tgaatgttgc aagtaaacaa tatgaaacgc tagttaaacg tttaaaaact gaacgaacac tctgggaaga tcctgacttt ccagctaatg ataaagcaat tgggaatctg ccagatttca gagagagaat tgaatggaaa agaccacatg aaattaatcc aaatgctaaa tttttcgctg gtggagcaag tcgtttcgat attgaacaag gagcacttgg tgactgctgg ttacttgcag tagtggcttc catatcagga tacccacagc tatttgatca agtggtgcct aaagatcaag aattgaaagg accagaatat gttggtgttg tacgttttcg gttttggcgt tttggtcact gggtggaagt gcttatagat gatcgtcttc cggttcgtca aggacgtaat acactcgttt ttatgcattc taatgatccc acggaattct ggtcagctct gttggaaaaa gcttatgcaa aattgaatgg ttgttatgct catctgagtg gaggatcaca aagtgaagct atggaagatt taactggtgg aatttgttta tctcttgaac tgaaccaaaa agaacgtcca agtgatctta tagatcagct taaaatttat gctcaacgtt gttgccttat gggttgtagt attgattcat cagttatgga acaaaaaatg gataatggac ttataggtag tcacgcatac agtttaacag gtgtatatcc cgtcaactac cgaggtcgca ctcaatggtt aatgcgttta cgtaatccat ggggtgacag tcatgaatgg aaaggtgcat ggtgtgatgg ttcacctcaa tggcgtgaaa taagtgaaca agaaaagaaa aatattaact tgagcttcac tgcagatgga gaattttgga tgtcatatga agattttgtt acttgttttt cacgagttga agtatgtcat ttaggtcttg aaagtttaga gtataatcaa aatttccatg ggaaaagaag attagacgaa gctatatttt ctggtcaatg gcaacgtaac gttaatgctg gtggttgtat taataatcgc actacctatt ggacgaatcc acaatttcgc atcacagttg aagattgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for ScmaA.19677.a.B7.GE42772
HARVESTED ON: 11/5/2018
SEQUENCED ON: 11/7/2018
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN VASKQYETLV KRLKTERTLW EDPDFPANDK AIGNLPDFRE RIEWKRPHEI NPNAKFFAGG ASRFDIEQGA LGDCWLLAVV ASISGYPQLF DQVVPKDQEL KGPEYVGVVR FRFWRFGHWV EVLIDDRLPV RQGRNTLVFM HSNDPTEFWS ALLEKAYAKL NGCYAHLSGG SQSEAMEDLT GGICLSLELN QKERPSDLID QLKIYAQRCC LMGCSIDSSV MEQKMDNGLI GSHAYSLTGV YPVNYRGRTQ WLMRLRNPWG DSHEWKGAWC DGSPQWREIS EQEKKNINLS FTADGEFWMS YEDFVTCFSR VEVCHLGLES LEYNQNFHGK RRLDEAIFSG QWQRNVNAGG CINNRTTYWT NPQFRITVED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg ttgcaagtaa acaatatgaa acgctagtta aacgtttaaa aactgaacga acactctggg aagatcctga ctttccagct aatgataaag caattgggaa tctgccagat ttcagagaga gaattgaatg gaaaagacca catgaaatta atccaaatgc taaatttttc gctggtggag caagtcgttt cgatattgaa caaggagcac ttggtgactg ctggttactt gcagtagtgg cttccatatc aggataccca cagctatttg atcaagtggt gcctaaagat caagaattga aaggaccaga atatgttggt gttgtacgtt ttcggttttg gcgttttggt cactgggtgg aagtgcttat agatgatcgt cttccggttc gtcaaggacg taatacactc gtttttatgc attctaatga tcccacggaa ttctggtcag ctctgttgga aaaagcttat gcaaaattga atggttgtta tgctcatctg agtggaggat cacaaagtga agctatggaa gatttaactg gtggaatttg tttatctctt gaactgaacc aaaaagaacg tccaagtgat cttatagatc agcttaaaat ttatgctcaa cgttgttgcc ttatgggttg tagtattgat tcatcagtta tggaacaaaa aatggataat ggacttatag gtagtcacgc atacagttta acaggtgtat atcccgtcaa ctaccgaggt cgcactcaat ggttaatgcg tttacgtaat ccatggggtg acagtcatga atggaaaggt gcatggtgtg atggttcacc tcaatggcgt gaaataagtg aacaagaaaa gaaaaatatt aacttgagct tcactgcaga tggagaattt tggatgtcat atgaagattt tgttacttgt ttttcacgag ttgaagtatg tcatttaggt cttgaaagtt tagagtataa tcaaaatttc catgggaaaa gaagattaga cgaagctata ttttctggtc aatggcaacg taacgttaat gctggtggtt gtattaataa tcgcactacc tattggacga atccacaatt tcgcatcaca gttgaagatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for ScmaA.19677.a.B4.PW38622
PURIFICATION DATe: 5/16/2019
CONCENTRATION: 13.88mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 1
VIAL VOLUME: 55µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHME FNPYLPKTLT PKGYAKFKLM MNVASKQYET LVKRLKTERT LWEDPDFPAN DKAIGNLPDF RERIEWKRPH EINPNAKFFA GGASRFDIEQ GALGDCWLLA VVASISGYPQ LFDQVVPKDQ ELKGPEYVGV VRFRFWRFGH WVEVLIDDRL PVRQGRNTLV FMHSNDPTEF WSALLEKAYA KLNGCYAHLS GGSQSEAMED LTGGICLSLE LNQKERPSDL IDQLKIYAQR CCLMGCSIDS SVMEQKMDNG LIGSHAYSLT GVYPVNYRGR TQWLMRLRNP WGDSHEWKGA WCDGSPQWRE ISEQEKKNIN LSFTADGEFW MSYEDFVTCF SRVEVCHLGL ESLEYNQNFH GKRRLDEAIF SGQWQRNVNA GGCINNRTTY WTNPQFRITV ED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGAAT TTAATCCATA TCTTCCTAAA ACACTTACTC CAAAAGGATA TGCTAAGTTT AAACTAATGA TGAATGTTGC AAGTAAACAA TATGAAACGC TAGTTAAACG TTTAAAAACT GAACGAACAC TCTGGGAAGA TCCTGACTTT CCAGCTAATG ATAAAGCAAT TGGGAATCTG CCAGATTTCA GAGAGAGAAT TGAATGGAAA AGACCACATG AAATTAATCC AAATGCTAAA TTTTTCGCTG GTGGAGCAAG TCGTTTCGAT ATTGAACAAG GAGCACTTGG TGACTGCTGG TTACTTGCAG TAGTGGCTTC CATATCAGGA TACCCACAGC TATTTGATCA AGTGGTGCCT AAAGATCAAG AATTGAAAGG ACCAGAATAT GTTGGTGTTG TACGTTTTCG GTTTTGGCGT TTTGGTCACT GGGTGGAAGT GCTTATAGAT GATCGTCTTC CGGTTCGTCA AGGACGTAAT ACACTCGTTT TTATGCATTC TAATGATCCC ACGGAATTCT GGTCAGCTCT GTTGGAAAAA GCTTATGCAA AATTGAATGG TTGTTATGCT CATCTGAGTG GAGGATCACA AAGTGAAGCT ATGGAAGATT TAACTGGTGG AATTTGTTTA TCTCTTGAAC TGAACCAAAA AGAACGTCCA AGTGATCTTA TAGATCAGCT TAAAATTTAT GCTCAACGTT GTTGCCTTAT GGGTTGTAGT ATTGATTCAT CAGTTATGGA ACAAAAAATG GATAATGGAC TTATAGGTAG TCACGCATAC AGTTTAACAG GTGTATATCC CGTCAACTAC CGAGGTCGCA CTCAATGGTT AATGCGTTTA CGTAATCCAT GGGGTGACAG TCATGAATGG AAAGGTGCAT GGTGTGATGG TTCACCTCAA TGGCGTGAAA TAAGTGAACA AGAAAAGAAA AATATTAACT TGAGCTTCAC TGCAGATGGA GAATTTTGGA TGTCATATGA AGATTTTGTT ACTTGTTTTT CACGAGTTGA AGTATGTCAT TTAGGTCTTG AAAGTTTAGA GTATAATCAA AATTTCCATG GGAAAAGAAG ATTAGACGAA GCTATATTTT CTGGTCAATG GCAACGTAAC GTTAATGCTG GTGGTTGTAT TAATAATCGC ACTACCTATT GGACGAATCC ACAATTTCGC ATCACAGTTG AAGATTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
Details for ScmaA.19677.a.B7.PW38600
PURIFICATION DATe: 2/7/2019
CONCENTRATION: 22.36mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 14
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMN VASKQYETLV KRLKTERTLW EDPDFPANDK AIGNLPDFRE RIEWKRPHEI NPNAKFFAGG ASRFDIEQGA LGDCWLLAVV ASISGYPQLF DQVVPKDQEL KGPEYVGVVR FRFWRFGHWV EVLIDDRLPV RQGRNTLVFM HSNDPTEFWS ALLEKAYAKL NGCYAHLSGG SQSEAMEDLT GGICLSLELN QKERPSDLID QLKIYAQRCC LMGCSIDSSV MEQKMDNGLI GSHAYSLTGV YPVNYRGRTQ WLMRLRNPWG DSHEWKGAWC DGSPQWREIS EQEKKNINLS FTADGEFWMS YEDFVTCFSR VEVCHLGLES LEYNQNFHGK RRLDEAIFSG QWQRNVNAGG CINNRTTYWT NPQFRITVED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG TTGCAAGTAA ACAATATGAA ACGCTAGTTA AACGTTTAAA AACTGAACGA ACACTCTGGG AAGATCCTGA CTTTCCAGCT AATGATAAAG CAATTGGGAA TCTGCCAGAT TTCAGAGAGA GAATTGAATG GAAAAGACCA CATGAAATTA ATCCAAATGC TAAATTTTTC GCTGGTGGAG CAAGTCGTTT CGATATTGAA CAAGGAGCAC TTGGTGACTG CTGGTTACTT GCAGTAGTGG CTTCCATATC AGGATACCCA CAGCTATTTG ATCAAGTGGT GCCTAAAGAT CAAGAATTGA AAGGACCAGA ATATGTTGGT GTTGTACGTT TTCGGTTTTG GCGTTTTGGT CACTGGGTGG AAGTGCTTAT AGATGATCGT CTTCCGGTTC GTCAAGGACG TAATACACTC GTTTTTATGC ATTCTAATGA TCCCACGGAA TTCTGGTCAG CTCTGTTGGA AAAAGCTTAT GCAAAATTGA ATGGTTGTTA TGCTCATCTG AGTGGAGGAT CACAAAGTGA AGCTATGGAA GATTTAACTG GTGGAATTTG TTTATCTCTT GAACTGAACC AAAAAGAACG TCCAAGTGAT CTTATAGATC AGCTTAAAAT TTATGCTCAA CGTTGTTGCC TTATGGGTTG TAGTATTGAT TCATCAGTTA TGGAACAAAA AATGGATAAT GGACTTATAG GTAGTCACGC ATACAGTTTA ACAGGTGTAT ATCCCGTCAA CTACCGAGGT CGCACTCAAT GGTTAATGCG TTTACGTAAT CCATGGGGTG ACAGTCATGA ATGGAAAGGT GCATGGTGTG ATGGTTCACC TCAATGGCGT GAAATAAGTG AACAAGAAAA GAAAAATATT AACTTGAGCT TCACTGCAGA TGGAGAATTT TGGATGTCAT ATGAAGATTT TGTTACTTGT TTTTCACGAG TTGAAGTATG TCATTTAGGT CTTGAAAGTT TAGAGTATAA TCAAAATTTC CATGGGAAAA GAAGATTAGA CGAAGCTATA TTTTCTGGTC AATGGCAACG TAACGTTAAT GCTGGTGGTT GTATTAATAA TCGCACTACC TATTGGACGA ATCCACAATT TCGCATCACA GTTGAAGATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for ScmaA.19677.a.B7.PW38601
PURIFICATION DATe: 2/7/2019
CONCENTRATION: 21.99mg/ml
OBSERVED MW: 42kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 21
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMN VASKQYETLV KRLKTERTLW EDPDFPANDK AIGNLPDFRE RIEWKRPHEI NPNAKFFAGG ASRFDIEQGA LGDCWLLAVV ASISGYPQLF DQVVPKDQEL KGPEYVGVVR FRFWRFGHWV EVLIDDRLPV RQGRNTLVFM HSNDPTEFWS ALLEKAYAKL NGCYAHLSGG SQSEAMEDLT GGICLSLELN QKERPSDLID QLKIYAQRCC LMGCSIDSSV MEQKMDNGLI GSHAYSLTGV YPVNYRGRTQ WLMRLRNPWG DSHEWKGAWC DGSPQWREIS EQEKKNINLS FTADGEFWMS YEDFVTCFSR VEVCHLGLES LEYNQNFHGK RRLDEAIFSG QWQRNVNAGG CINNRTTYWT NPQFRITVED
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG TTGCAAGTAA ACAATATGAA ACGCTAGTTA AACGTTTAAA AACTGAACGA ACACTCTGGG AAGATCCTGA CTTTCCAGCT AATGATAAAG CAATTGGGAA TCTGCCAGAT TTCAGAGAGA GAATTGAATG GAAAAGACCA CATGAAATTA ATCCAAATGC TAAATTTTTC GCTGGTGGAG CAAGTCGTTT CGATATTGAA CAAGGAGCAC TTGGTGACTG CTGGTTACTT GCAGTAGTGG CTTCCATATC AGGATACCCA CAGCTATTTG ATCAAGTGGT GCCTAAAGAT CAAGAATTGA AAGGACCAGA ATATGTTGGT GTTGTACGTT TTCGGTTTTG GCGTTTTGGT CACTGGGTGG AAGTGCTTAT AGATGATCGT CTTCCGGTTC GTCAAGGACG TAATACACTC GTTTTTATGC ATTCTAATGA TCCCACGGAA TTCTGGTCAG CTCTGTTGGA AAAAGCTTAT GCAAAATTGA ATGGTTGTTA TGCTCATCTG AGTGGAGGAT CACAAAGTGA AGCTATGGAA GATTTAACTG GTGGAATTTG TTTATCTCTT GAACTGAACC AAAAAGAACG TCCAAGTGAT CTTATAGATC AGCTTAAAAT TTATGCTCAA CGTTGTTGCC TTATGGGTTG TAGTATTGAT TCATCAGTTA TGGAACAAAA AATGGATAAT GGACTTATAG GTAGTCACGC ATACAGTTTA ACAGGTGTAT ATCCCGTCAA CTACCGAGGT CGCACTCAAT GGTTAATGCG TTTACGTAAT CCATGGGGTG ACAGTCATGA ATGGAAAGGT GCATGGTGTG ATGGTTCACC TCAATGGCGT GAAATAAGTG AACAAGAAAA GAAAAATATT AACTTGAGCT TCACTGCAGA TGGAGAATTT TGGATGTCAT ATGAAGATTT TGTTACTTGT TTTTCACGAG TTGAAGTATG TCATTTAGGT CTTGAAAGTT TAGAGTATAA TCAAAATTTC CATGGGAAAA GAAGATTAGA CGAAGCTATA TTTTCTGGTC AATGGCAACG TAACGTTAAT GCTGGTGGTT GTATTAATAA TCGCACTACC TATTGGACGA ATCCACAATT TCGCATCACA GTTGAAGATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT