SatyA.18724.a

Flagellin FliC

CENTER ID: SatyA.18724.a
ORGANISM: Salmonella typhimurium LT2
ASSOCIATED DISEASE: salmonellosis
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
SatyA.18724.a.B11.GE37594 full length 1 495
SatyA.18724.a.B12.GE37595 full length 1 495
SatyA.18724.a.B13.GE37596 full length 1 495
SatyA.18724.a.B14.GE37597 full length 1 495
SatyA.18724.a.B15.GE37598 full length 1 495
SatyA.18724.a.B16.GE37599 full length 1 495
SatyA.18724.a.B17.GE37600 full length 1 495
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
SatyA.18724.a.B13.PW37521 full length 1 495

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|99287.12.peg.2074
RefSeq: NP_460912.1
UniProt: P06179

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAATVTG YADTTIALDN STFKASATGL GGTDQKIDGD LKFDDTTGKY YAKVTVTGGT GKDGYYEVSV DKTNGEVTLA GGATSPLTGG LPATATEDVK NVQVANADLT EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFNSAITNL GNTVNNLTSA RSRIEDSDYA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLR
NT Sequence
atggcacaag tcattaatac aaacagcctg tcgctgttga cccagaataa cctgaacaaa tcccagtccg ctctgggcac cgctatcgag cgtctgtctt ccggtctgcg tatcaacagc gcgaaagacg atgcggcagg tcaggcgatt gctaaccgtt ttaccgcgaa catcaaaggt ctgactcagg cttcccgtaa cgctaacgac ggtatctcca ttgcgcagac cactgaaggc gcgctgaacg aaatcaacaa caacctgcag cgtgtgcgtg aactggcggt tcagtctgct aacagcacca actcccagtc tgacctcgac tccatccagg ctgaaatcac ccagcgcctg aacgaaatcg accgtgtatc cggccagact cagttcaacg gcgtgaaagt cctggcgcag gacaacaccc tgaccatcca ggttggtgcc aacgacggtg aaactatcga tatcgatctg aagcagatca actctcagac cctgggtctg gatacgctga atgtgcaaca aaaatataag gtcagcgata cggctgcaac tgttacagga tatgccgata ctacgattgc tttagacaat agtactttta aagcctcggc tactggtctt ggtggtactg accagaaaat tgatggcgat ttaaaatttg atgatacgac tggaaaatat tacgccaaag ttaccgttac ggggggaact ggtaaagatg gctattatga agtttccgtt gataagacga acggtgaggt gactcttgct ggcggtgcga cttccccgct tacaggtgga ctacctgcga cagcaactga ggatgtgaaa aatgtacaag ttgcaaatgc tgatttgaca gaggctaaag ccgcattgac agcagcaggt gttaccggca cagcatctgt tgttaagatg tcttatactg ataataacgg taaaactatt gatggtggtt tagcagttaa ggtaggcgat gattactatt ctgcaactca aaataaagat ggttccataa gtattaatac tacgaaatac actgcagatg acggtacatc caaaactgca ctaaacaaac tgggtggcgc agacggcaaa accgaagttg tttctattgg tggtaaaact tacgctgcaa gtaaagccga aggtcacaac tttaaagcac agcctgatct ggcggaagcg gctgctacaa ccaccgaaaa cccgctgcag aaaattgatg ctgctttggc acaggttgac acgttacgtt ctgacctggg tgcggtacag aaccgtttca actccgctat taccaacctg ggcaacaccg taaacaacct gacttctgcc cgtagccgta tcgaagattc cgactacgcg accgaagttt ccaacatgtc tcgcgcgcag attctgcagc aggccggtac ctccgttctg gcgcaggcga accaggttcc gcaaaacgtc ctctctttac tgcgttaa
Details for SatyA.18724.a.B11.GE37594
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAI ANRFTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYK VSDTAANSTE AKAALTAAGV TGTASVVKMS YTDNNGKTID GGLAVKVGDD YYSATQNKDG SISINTTKYT ADDGTSKTAL NKLGGADGKT EVVSIGGKTY AASKAEGHNF KAQPDLAEAA ATTTENPLQK IDAALAQVDT LRSDLGAVQN RFNSAITNLG NTVNNLTSAR SRIEDSDYAT EVSNMSRAQI LQQAGTSVLA QANQVP
Validated NT Sequence
gcgggacttg atttgcctgt gccaggactg aggtacctgc ttgctgcaga atctgagcac gcgacatatt tgaaacttcg gtcgcatagt cgctatcttc gatgcgggaa cgagcactcg tcaggttatt aacggtgttg cccagattcg taatcgcgga gttgaagcga ttctgcacag cacccaggtc actacgcagg gtatcgactt gcgccagtgc ggcatcaatt ttctgcagcg gattttcggt cgtggtcgca gctgcttccg ccagatccgg ctgagcctta aagttatgac cttcggcctt agaggccgcg tacgttttac cgccgatgct cacgacttcg gttttgccat ctgcaccgcc cagcttattc agggcggttt tgctcgtacc gtcatccgcg gtatatttcg tggtgttgat cgaaattgag ccatccttat tctgggttgc actgtaatag tcatcaccaa ctttcacggc cagaccgccg tcaatcgtct tgccgttatt atcggtgtaa gacattttaa ccacgctggc ggtacccgtc acaccagctg cggtcagtgc tgcttttgct tcggtggagt tagctgccgt gtcactaact ttatattttt gctgcacatt cagggtatcc aggcccagcg tctgagagtt gatttgtttc aggtcgatat caatggtttc accgtcattt gcgccaacct gaatggtcag cgtgttatct tgggccagga ctttaacacc attgaattgc gtctggccag acacgcgatc aatttcattc agacgctggg tgatttcggc ttgaatgctg tccagatcgg attgactgtt ggtagaattt gcgctctgga cggccagttc gcgaacacgt tgcaggttat tgttgatttc gttcagtgca ccttccgtgg tctgggcgat cgaaatgcca tcgttcgcat tgcgtgaagc ctgcgtcaga cctttgatgt tcgcggtaaa acgattcgca atagcctgac cggccgcgtc atcctttgcg gagttgatgc gcaggccaga gctcagacgt tcgattgccg tgcccagagc ggattgcgat ttgttcagat tgttttgcgt cagcagggac aggctattcg tattgataac ttgtgccat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF NGVKVLAQDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYKVS DTAANSTEAK AALTAAGVTG TASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA SKAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF NSAITNLGNT VNNLTSARSR IEDSDYATEV SNMSRAQILQ QAGTSVLAQA NQVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggcaca agttatcaat acgaatagcc tgtccctgct gacgcaaaac aatctgaaca aatcgcaatc cgctctgggc acggcaatcg aacgtctgag ctctggcctg cgcatcaact ccgcaaagga tgacgcggcc ggtcaggcta ttgcgaatcg ttttaccgcg aacatcaaag gtctgacgca ggcttcacgc aatgcgaacg atggcatttc gatcgcccag accacggaag gtgcactgaa cgaaatcaac aataacctgc aacgtgttcg cgaactggcc gtccagagcg caaattctac caacagtcaa tccgatctgg acagcattca agccgaaatc acccagcgtc tgaatgaaat tgatcgcgtg tctggccaga cgcaattcaa tggtgttaaa gtcctggccc aagataacac gctgaccatt caggttggcg caaatgacgg tgaaaccatt gatatcgacc tgaaacaaat caactctcag acgctgggcc tggataccct gaatgtgcag caaaaatata aagttagtga cacggcagct aactccaccg aagcaaaagc agcactgacc gcagctggtg tgacgggtac cgccagcgtg gttaaaatgt cttacaccga taataacggc aagacgattg acggcggtct ggccgtgaaa gttggtgatg actattacag tgcaacccag aataaggatg gctcaatttc gatcaacacc acgaaatata ccgcggatga cggtacgagc aaaaccgccc tgaataagct gggcggtgca gatggcaaaa ccgaagtcgt gagcatcggc ggtaaaacgt acgcggcctc taaggccgaa ggtcataact ttaaggctca gccggatctg gcggaagcag ctgcgaccac gaccgaaaat ccgctgcaga aaattgatgc cgcactggcg caagtcgata ccctgcgtag tgacctgggt gctgtgcaga atcgcttcaa ctccgcgatt acgaatctgg gcaacaccgt taataacctg acgagtgctc gttcccgcat cgaagatagc gactatgcga ccgaagtttc aaatatgtcg cgtgctcaga ttctgcagca agcaggtacc tcagtcctgg cacaggcaaa tcaagtcccg cagaacgtgc tgtcgctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B12.GE37595
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP GMAQVINTNS LSLLTQNNLN KSQSALGTAI ERLSSGLRIN SAKDDAAGQA IANRFTANIK GLTQASRNAN DGISIAQTTE GALNEINNNL QRVRELAVQS ANSTNSQSDL DSIQAEITQR LNEIDRVSGQ TQFNGVKVLA QDNTLTIQVG ANDGETIDID LKQINSQTLG LDTLNVQQKY KVSDTAANST EAKAALTAAG VTGTASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAASKAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFDSAIENL GNTVNNLTSA RSRIEDSDTA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccc gggatggcac aagtcatcaa taccaatagc ctgagcctgc tgacgcaaaa caacctgaac aaatcgcaat cggcactggg caccgcaatc gaacgtctga gctctggcct gcgcatcaac tccgcaaagg atgacgcggc cggtcaggct attgcgaatc gttttacggc gaacatcaaa ggtctgaccc aggcttcacg caatgcgaac gatggcattt cgatcgccca gaccacggaa ggtgcactga acgaaatcaa caataacctg caacgtgttc gcgaactggc cgtccagagc gcaaattcta ccaacagtca atccgatctg gacagcattc aagccgaaat cacgcagcgt ctgaatgaaa ttgatcgcgt gtctggccag acccaattca atggtgttaa agtcctggcc caagataaca cgctgaccat tcaggttggc gcaaatgacg gtgaaaccat tgatatcgac ctgaaacaaa tcaactctca gacgctgggc ctggataccc tgaatgtgca gcaaaaatat aaagttagtg acacggcagc taactccacc gaagcaaaag cagcactgac cgcagctggt gtgacgggta ccgccagcgt ggttaaaatg tcttacacgg ataataacgg caagaccatt gacggcggtc tggccgtgaa agttggtgat gactattaca gtgcaaccca gaataaggat ggctcaattt cgatcaacac cacgaaatat accgcggatg acggtacgag caaaaccgcc ctgaataagc tgggcggtgc agatggcaaa acggaagtcg tgagcatcgg cggtaaaacc tacgcggcct ctaaggccga aggtcataac tttaaggctc agccggatct ggcggaagca gctgcgacca cgaccgaaaa tccgctgcag aaaattgatg ccgcactggc gcaagtcgat accctgcgta gtgacctggg tgctgtgcag aaccgcttcg attccgcgat tgaaaatctg ggcaacacgg ttaataacct gaccagtgct cgttcccgca tcgaagatag cgacacggcg accgaagttt caaatatgtc gcgtgctcag attctgcagc aagcaggtac ctcagtcctg gcacaggcaa atcaagtccc gcagaacgtg ctgtcgctgc tgcgctaagt gagtaagat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF NGVKVLAQDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYKVS DTAANSTEAK AALTAAGVTG TASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA SKAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DSAIENLGNT VNNLTSARSR IEDSDTATEV SNMSRAQILQ QAGTSVLAQA NQVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggcaca agtcatcaat accaatagcc tgagcctgct gacgcaaaac aacctgaaca aatcgcaatc ggcactgggc accgcaatcg aacgtctgag ctctggcctg cgcatcaact ccgcaaagga tgacgcggcc ggtcaggcta ttgcgaatcg ttttacggcg aacatcaaag gtctgaccca ggcttcacgc aatgcgaacg atggcatttc gatcgcccag accacggaag gtgcactgaa cgaaatcaac aataacctgc aacgtgttcg cgaactggcc gtccagagcg caaattctac caacagtcaa tccgatctgg acagcattca agccgaaatc acgcagcgtc tgaatgaaat tgatcgcgtg tctggccaga cccaattcaa tggtgttaaa gtcctggccc aagataacac gctgaccatt caggttggcg caaatgacgg tgaaaccatt gatatcgacc tgaaacaaat caactctcag acgctgggcc tggataccct gaatgtgcag caaaaatata aagttagtga cacggcagct aactccaccg aagcaaaagc agcactgacc gcagctggtg tgacgggtac cgccagcgtg gttaaaatgt cttacacgga taataacggc aagaccattg acggcggtct ggccgtgaaa gttggtgatg actattacag tgcaacccag aataaggatg gctcaatttc gatcaacacc acgaaatata ccgcggatga cggtacgagc aaaaccgccc tgaataagct gggcggtgca gatggcaaaa cggaagtcgt gagcatcggc ggtaaaacct acgcggcctc taaggccgaa ggtcataact ttaaggctca gccggatctg gcggaagcag ctgcgaccac gaccgaaaat ccgctgcaga aaattgatgc cgcactggcg caagtcgata ccctgcgtag tgacctgggt gctgtgcaga accgcttcga ttccgcgatt gaaaatctgg gcaacacggt taataacctg accagtgctc gttcccgcat cgaagatagc gacacggcga ccgaagtttc aaatatgtcg cgtgctcaga ttctgcagca agcaggtacc tcagtcctgg cacaggcaaa tcaagtcccg cagaacgtgc tgtcgctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B13.GE37596
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP GMAQVINTNS LSLLTQNNLN KSQSALGTAI ERLSSGLRIN SAKDDAAGQA IANRFTANIK GLTQASRNAN DGISIAQTTE GALNEINNNL QRVRELAVQS ANSTNSQSDL DSIQAEITQR LNEIDRVSGQ TQFNGVKVLA QDNTLTIQVG ANDGETIDID LKQINSQTLG LDTLNVQQKY DVSDTAANST EAKAALTRAG VTGDASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAAKMAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFDSAIENL GNTVNNLTSA RSRIEDSDTA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccc gggatggcac aagtcatcaa caccaactcg ctgagcctgc tgacgcaaaa caacctgaat aagtcgcaat cggcactggg cacggcaatc gaacgtctga gctctggcct gcgcatcaac tccgcaaagg atgacgcagc cggtcaggcc attgcaaatc gttttacggc caacatcaaa ggtctgaccc aggcctcacg caatgcaaac gatggcattt cgatcgctca gaccacggaa ggtgcgctga acgaaatcaa caataacctg caacgtgttc gcgaactggc tgtccagagc gcgaattcta ccaacagtca atccgatctg gactcaattc aagccgaaat cacgcagcgt ctgaatgaaa ttgatcgcgt gtcgggccag acccaattca atggtgttaa agtcctggcc caagataaca cgctgaccat tcaggttggc gcaaatgacg gtgaaaccat tgatatcgac ctgaaacaaa tcaacagtca gacgctgggc ctggataccc tgaatgtgca gcaaaagtat gatgttagtg acacggcagc taactccacc gaagccaaag cagcactgac gcgtgccggt gtgaccggtg atgcaagcgt ggttaaaatg tcttacacgg ataataacgg caagaccatt gacggcggtc tggccgtgaa agttggtgat gactattaca gcgcaaccca gaataaggat ggctcaattt cgatcaacac cacgaaatat accgcggatg acggtacgag caaaaccgcc ctgaataagc tgggcggtgc agatggcaaa acggaagtcg tgtctatcgg cggtaaaacc tacgcagcta agatggcaga aggtcataac tttaaggcac agccggatct ggcagaagca gcagcaacca cgaccgaaaa tccgctgcag aaaattgacg ctgcgctggc gcaagtcgat accctgcgta gcgacctggg tgctgtgcag aaccgcttcg attctgcgat tgaaaatctg ggcaacacgg ttaataacct gaccagtgct cgttcccgca tcgaagattc tgacacggcg accgaagttt caaatatgtc gcgtgctcag attctgcagc aagcgggcac cagtgtcctg gctcaggcga atcaagtccc gcagaacgtg ctgtccctgc tgcgctaagt gagtaagat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF NGVKVLAQDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYDVS DTAANSTEAK AALTRAGVTG DASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA KMAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DSAIENLGNT VNNLTSARSR IEDSDTATEV SNMSRAQILQ QAGTSVLAQA NQVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggcaca agtcatcaac accaactcgc tgagcctgct gacgcaaaac aacctgaata agtcgcaatc ggcactgggc acggcaatcg aacgtctgag ctctggcctg cgcatcaact ccgcaaagga tgacgcagcc ggtcaggcca ttgcaaatcg ttttacggcc aacatcaaag gtctgaccca ggcctcacgc aatgcaaacg atggcatttc gatcgctcag accacggaag gtgcgctgaa cgaaatcaac aataacctgc aacgtgttcg cgaactggct gtccagagcg cgaattctac caacagtcaa tccgatctgg actcaattca agccgaaatc acgcagcgtc tgaatgaaat tgatcgcgtg tcgggccaga cccaattcaa tggtgttaaa gtcctggccc aagataacac gctgaccatt caggttggcg caaatgacgg tgaaaccatt gatatcgacc tgaaacaaat caacagtcag acgctgggcc tggataccct gaatgtgcag caaaagtatg atgttagtga cacggcagct aactccaccg aagccaaagc agcactgacg cgtgccggtg tgaccggtga tgcaagcgtg gttaaaatgt cttacacgga taataacggc aagaccattg acggcggtct ggccgtgaaa gttggtgatg actattacag cgcaacccag aataaggatg gctcaatttc gatcaacacc acgaaatata ccgcggatga cggtacgagc aaaaccgccc tgaataagct gggcggtgca gatggcaaaa cggaagtcgt gtctatcggc ggtaaaacct acgcagctaa gatggcagaa ggtcataact ttaaggcaca gccggatctg gcagaagcag cagcaaccac gaccgaaaat ccgctgcaga aaattgacgc tgcgctggcg caagtcgata ccctgcgtag cgacctgggt gctgtgcaga accgcttcga ttctgcgatt gaaaatctgg gcaacacggt taataacctg accagtgctc gttcccgcat cgaagattct gacacggcga ccgaagtttc aaatatgtcg cgtgctcaga ttctgcagca agcgggcacc agtgtcctgg ctcaggcgaa tcaagtcccg cagaacgtgc tgtccctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B14.GE37597
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 43kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP GMAQVINTNS LSLLTQNNLN KSQSALGTAI ERLSSGLRIN SAKDDAAGQA IANRFTANIK GLTQASRNAN DGISIAQTTE GALNEINNNL QRVRELAVQS ANSTNSQSDL DSIQAEITQR LNEIDRVSGQ TQFKGVKVLA EDNTLTIQVG ANDGETIDID LKQINSQTLG LDTLNVQQKY DVSDTAANST EAKAALTRAG VTGDASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAAKMAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFDSAIENL GNTVNNLTSA RSRIEDSDTA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccc gggatggctc aagtcatcaa caccaactct ctgtcgctgc tgacgcaaaa taacctgaat aagtcgcaat ccgcactggg caccgcaatc gaacgtctga gctctggcct gcgcatcaat tccgcaaagg atgacgcagc cggtcaggca attgcaaatc gttttacggc caacatcaaa ggtctgaccc aggcctcacg caatgcaaac gatggcattt cgatcgctca gaccacggaa ggtgcgctga atgaaatcaa caataacctg caacgtgttc gcgaactggc tgtccagagc gcgaattcta ccaacagtca atccgatctg gactcaattc aagccgaaat cacgcagcgt ctgaacgaaa ttgatcgcgt gtcgggccag acccaattca aaggtgttaa ggtcctggcc gaagataata cgctgaccat tcaggttggc gcaaacgacg gtgaaaccat tgatatcgac ctgaaacaaa tcaatagtca gacgctgggc ctggataccc tgaacgtgca gcaaaagtat gatgttagtg acacggcagc taattccacc gaagccaaag cagcactgac gcgtgccggt gtgaccggtg atgcaagcgt ggttaaaatg tcttacacgg ataataacgg caagaccatt gacggcggtc tggccgtgaa agttggtgat gactattaca gcgcaaccca gaataaggat ggctcaattt cgatcaacac cacgaaatat accgcggatg acggtacgag caaaaccgcc ctgaacaagc tgggcggtgc agatggcaaa acggaagtcg tgtctatcgg cggtaaaacc tacgcagcta agatggcaga aggtcataat tttaaagcac agccggatct ggcagaagca gcagcaacca cgaccgaaaa cccgctgcag aaaattgacg ctgcgctggc gcaagtcgat accctgcgta gcgacctggg tgctgtgcag aatcgcttcg attctgcgat tgaaaatctg ggcaacacgg ttaataacct gaccagtgct cgttcccgca tcgaagattc tgacacggcg accgaagttt caaacatgtc gcgtgctcag attctgcagc aagcgggcac cagtgtcctg gctcaggcga atcaagtccc gcagaacgtg ctgtccctgc tgcgctaagt gagtaagat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF KGVKVLAEDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYDVS DTAANSTEAK AALTRAGVTG DASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA KMAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DSAIENLGNT VNNLTSARSR IEDSDTATEV SNMSRAQILQ QAGTSVLAQA NQVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggctca agtcatcaac accaactctc tgtcgctgct gacgcaaaat aacctgaata agtcgcaatc cgcactgggc accgcaatcg aacgtctgag ctctggcctg cgcatcaatt ccgcaaagga tgacgcagcc ggtcaggcaa ttgcaaatcg ttttacggcc aacatcaaag gtctgaccca ggcctcacgc aatgcaaacg atggcatttc gatcgctcag accacggaag gtgcgctgaa tgaaatcaac aataacctgc aacgtgttcg cgaactggct gtccagagcg cgaattctac caacagtcaa tccgatctgg actcaattca agccgaaatc acgcagcgtc tgaacgaaat tgatcgcgtg tcgggccaga cccaattcaa aggtgttaag gtcctggccg aagataatac gctgaccatt caggttggcg caaacgacgg tgaaaccatt gatatcgacc tgaaacaaat caatagtcag acgctgggcc tggataccct gaacgtgcag caaaagtatg atgttagtga cacggcagct aattccaccg aagccaaagc agcactgacg cgtgccggtg tgaccggtga tgcaagcgtg gttaaaatgt cttacacgga taataacggc aagaccattg acggcggtct ggccgtgaaa gttggtgatg actattacag cgcaacccag aataaggatg gctcaatttc gatcaacacc acgaaatata ccgcggatga cggtacgagc aaaaccgccc tgaacaagct gggcggtgca gatggcaaaa cggaagtcgt gtctatcggc ggtaaaacct acgcagctaa gatggcagaa ggtcataatt ttaaagcaca gccggatctg gcagaagcag cagcaaccac gaccgaaaac ccgctgcaga aaattgacgc tgcgctggcg caagtcgata ccctgcgtag cgacctgggt gctgtgcaga atcgcttcga ttctgcgatt gaaaatctgg gcaacacggt taataacctg accagtgctc gttcccgcat cgaagattct gacacggcga ccgaagtttc aaacatgtcg cgtgctcaga ttctgcagca agcgggcacc agtgtcctgg ctcaggcgaa tcaagtcccg cagaacgtgc tgtccctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B15.GE37598
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMP GMAQVINTNS LSLLTQNNLN KSQSALGTAI ERLSSGLRIN SAKDDAAGQA IANRFTANIK GLTQASRNAN DGISIAQTTE GALNEINNNL QRVRELAVQS ANSTNSQSDL DSIQAEITQR LNEIDRVSGQ TQFKGVKVLA EDNTLTIQVG ANDGETIDID LKQINSQTLG LDTLNVQQKY DVSDTAANST EAKAALTRAG VTGDASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAAKMAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFDQAIDDL GNTVNNLTSA RSRIEDSDTA TEVSEMSRAQ ILQQAGTSVL AKENAVPQNV LSLLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccc gggatggcac aagttatcaa taccaactcg ctgtcgctgc tgacgcaaaa caacctgaac aagtcgcaat ccgctctggg caccgctatc gaacgtctga gctctggcct gcgcatcaat agcgcaaagg atgacgcagc cggtcaggca attgcaaatc gttttacggc caacatcaaa ggtctgaccc aagcttcacg caatgcgaac gatggcattt cgatcgctca gaccacggaa ggtgcgctga atgaaatcaa caataacctg cagcgtgttc gcgaactggc cgtccaaagc gcaaattcta ccaacagtca gtccgatctg gacagtattc aggccgaaat cacgcaacgt ctgaacgaaa ttgatcgcgt gtccggccag acccaattca aaggtgttaa ggtcctggcc gaagataata cgctgaccat tcaggttggc gcaaacgacg gtgaaaccat tgatatcgac ctgaaacaga tcaatagtca aacgctgggc ctggataccc tgaacgtgca gcaaaagtat gatgttagtg acacggcagc taattccacc gaagccaaag cagcactgac gcgtgccggt gtgaccggtg atgcaagcgt ggttaaaatg tcttacacgg ataataacgg caagaccatt gacggcggtc tggccgtgaa agttggtgat gactattaca gcgcaaccca gaataaggat ggctcaattt cgatcaacac cacgaaatat accgcggatg acggcacgtc aaaaaccgcc ctgaacaagc tgggcggtgc agatggcaaa acggaagtcg tgtcgatcgg cggtaaaacc tacgcagcta agatggcaga aggtcataat tttaaagcac agccggatct ggcagaagca gcagcaacca cgaccgaaaa cccgctgcaa aaaattgacg ctgcactggc acaggtcgat accctgcgtt cagacctggg tgctgtgcag aatcgcttcg atcaagcgat tgatgacctg ggcaacacgg ttaataacct gaccagtgct cgttcccgca tcgaagattc tgacacggcg accgaagttt cagaaatgtc gcgtgctcag attctgcagc aagcgggcac cagcgtcctg gctaaagaaa atgcggtccc gcagaacgtg ctgtctctgc tgcgctaagt gagtaagat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF KGVKVLAEDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYDVS DTAANSTEAK AALTRAGVTG DASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA KMAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DQAIDDLGNT VNNLTSARSR IEDSDTATEV SEMSRAQILQ QAGTSVLAKE NAVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggcaca agttatcaat accaactcgc tgtcgctgct gacgcaaaac aacctgaaca agtcgcaatc cgctctgggc accgctatcg aacgtctgag ctctggcctg cgcatcaata gcgcaaagga tgacgcagcc ggtcaggcaa ttgcaaatcg ttttacggcc aacatcaaag gtctgaccca agcttcacgc aatgcgaacg atggcatttc gatcgctcag accacggaag gtgcgctgaa tgaaatcaac aataacctgc agcgtgttcg cgaactggcc gtccaaagcg caaattctac caacagtcag tccgatctgg acagtattca ggccgaaatc acgcaacgtc tgaacgaaat tgatcgcgtg tccggccaga cccaattcaa aggtgttaag gtcctggccg aagataatac gctgaccatt caggttggcg caaacgacgg tgaaaccatt gatatcgacc tgaaacagat caatagtcaa acgctgggcc tggataccct gaacgtgcag caaaagtatg atgttagtga cacggcagct aattccaccg aagccaaagc agcactgacg cgtgccggtg tgaccggtga tgcaagcgtg gttaaaatgt cttacacgga taataacggc aagaccattg acggcggtct ggccgtgaaa gttggtgatg actattacag cgcaacccag aataaggatg gctcaatttc gatcaacacc acgaaatata ccgcggatga cggcacgtca aaaaccgccc tgaacaagct gggcggtgca gatggcaaaa cggaagtcgt gtcgatcggc ggtaaaacct acgcagctaa gatggcagaa ggtcataatt ttaaagcaca gccggatctg gcagaagcag cagcaaccac gaccgaaaac ccgctgcaaa aaattgacgc tgcactggca caggtcgata ccctgcgttc agacctgggt gctgtgcaga atcgcttcga tcaagcgatt gatgacctgg gcaacacggt taataacctg accagtgctc gttcccgcat cgaagattct gacacggcga ccgaagtttc agaaatgtcg cgtgctcaga ttctgcagca agcgggcacc agcgtcctgg ctaaagaaaa tgcggtcccg cagaacgtgc tgtctctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B16.GE37599
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 66
Validated AA Sequence
MAQVINTNSL SLLTQNNLNK SQSALGTAIE RLSSGLRINS AKDDAAGQAE ANADTANIKG LTQASRNAND GISIAQTTEG ALNEINNNLQ RVRELAVQSA NSTNSQSDLD SIQAEITQRL NEIDRVSGQT QFNGVKVLAQ DNTLTIQVGA NDGETIDIDL KQINSQTLGL DTLNVQQKYD VSDQAANSTE AKAALTAAGV TGTASVVKMS YTDNNGKTID GGLAVKVGDD YYSANKTKDG SISINTTKYT ADDGTSKTAL NKLGRC
Validated NT Sequence
cgggacggca ttttccttcg ccaggacttt ggtgccggct tgctgcagaa tctgcgcacg cgacatgttt gaaacttcgg tattgtcatc actatcttcg atgcgggaac gtgcactggt caggttatta accgtattgc ccaggtcgga aatagcttga tcgaagcggt tctgcactgc acccaggtca ctacgcaggg tatcgacctg ggccagcgca gcatcaattt tttgcagcgg attttcggtc gtggttgcgg ccgcttcctt cannttcggc tgggctttga agttatgacc ttccnncttn agacttttta aaggttttac cgccgatgct cacgacttcc gttttgccat cagcaccgcc ccagcttatt cagtgcggtt ttagacgtgc cgtcatcggc ggtatatttc gtggtattga tcgaaattga gccatccttc gttttgttgg cgctgtaata gtcatcacca actttcaccg ccagaccgcc gtcaatggtc ttgccgttat tatccgtgta agacatttta accacgctgg cggtacccgt cacaccagct gcggtcagtg ctgcttttgc ttccgtggag ttagctgcct ggtcactaac atcatacttt tgctgcacat tcagggtatc caggcccagc gtttgagagt tgatctgttt caggtcgata tcaatggttt caccgtcatt agcgccaact tgaatggtca gcgtgttatc ctgtgccagg actttaacac cattaaattg ggtctggccc gacacgcgat caatttcatt cagacgttgc gtgatttccg cctgaattga gtccagatcg gactgactgt tggtagaatt agcgctttgg actgccagtt cgcgaacacg ctgcaggtta ttgttgattt cgttcagagc accttccgtg gtctgtgcga tcgaaatgcc gtcgttggca ttacgtgacg cctgggtcag acctttaatg ttcgccgtat cagcatttgc ttctgcctga ccggctgcgt catcctttgc ggagttgatg cgcaggccag agctcagacg ttcgattgcg gtgcccagtg cggattgcga tttattcagg ttgttttgcg tcagcaggct caggctattg gtgttgatga cttgtgccat c
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAEAN ADTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF NGVKVLAQDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYDVS DQAANSTEAK AALTAAGVTG TASVVKMSYT DNNGKTIDGG LAVKVGDDYY SANKTKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTFKK SKAEGHNFKA QPKLKEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DQAISDLGNT VNNLTSARSR IEDSDDNTEV SNMSRAQILQ QAGTKVLAKE NAVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ggatggcaca agtcatcaac accaatagcc tgagcctgct gacgcaaaac aacctgaata aatcgcaatc cgcactgggc accgcaatcg aacgtctgag ctctggcctg cgcatcaact ccgcaaagga tgacgcagcc ggtcaggcag aagcaaatgc tgatacggcg aacattaaag gtctgaccca ggcgtcacgt aatgccaacg acggcatttc gatcgcacag accacggaag gtgctctgaa cgaaatcaac aataacctgc agcgtgttcg cgaactggca gtccaaagcg ctaattctac caacagtcag tccgatctgg actcaattca ggcggaaatc acgcaacgtc tgaatgaaat tgatcgcgtg tcgggccaga cccaatttaa tggtgttaaa gtcctggcac aggataacac gctgaccatt caagttggcg ctaatgacgg tgaaaccatt gatatcgacc tgaaacagat caactctcaa acgctgggcc tggataccct gaatgtgcag caaaagtatg atgttagtga ccaggcagct aactccacgg aagcaaaagc agcactgacc gcagctggtg tgacgggtac cgccagcgtg gttaaaatgt cttacacgga taataacggc aagaccattg acggcggtct ggcggtgaaa gttggtgatg actattacag cgccaacaaa acgaaggatg gctcaatttc gatcaatacc acgaaatata ccgccgatga cggcacgtct aaaaccgcac tgaataagct gggcggtgct gatggcaaaa cggaagtcgt gagcatcggc ggtaaaacct ttaaaaagtc taaggcggaa ggtcataact tcaaagccca gccgaaactg aaggaagcgg ccgcaaccac gaccgaaaat ccgctgcaaa aaattgatgc tgcgctggcc caggtcgata ccctgcgtag tgacctgggt gcagtgcaga accgcttcga tcaagctatt tccgacctgg gcaatacggt taataacctg accagtgcac gttcccgcat cgaagatagt gatgacaata ccgaagtttc aaacatgtcg cgtgcgcaga ttctgcagca agccggcacc aaagtcctgg cgaaggaaaa tgccgtcccg cagaacgtgc tgagcctgct gcgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SatyA.18724.a.B17.GE37600
HARVESTED ON: 11/25/2013
SEQUENCED ON: 12/4/2013
EXPECTED MW: 9kDa
OBSERVED MW: 9kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL AAKPSDPALE VSNMSRAQIL QQAGTSVLAQ ANQVPQNVLS LLR
Validated NT Sequence
atcttactca cttaacgcag cagtgacagg acattttgcg gaacttgatt cgcttgtgcc agaacggagg tgccagcttg ctgcagaatc tgtgcgcgag acatgttgct cacttccagg gccggatcac tcggtttggc cgccagacgt tcgatggccg tgcccagtgc cgattgcgat ttgttcagat tattttgcgt cagcaggctc aggctattgg tattgatgac ttgcgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL AAKPSDPALE VSNMSRAQIL QQAGTSVLAQ ANQVPQNVLS LLR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc aagtcatcaa taccaatagc ctgagcctgc tgacgcaaaa taatctgaac aaatcgcaat cggcactggg cacggccatc gaacgtctgg cggccaaacc gagtgatccg gccctggaag tgagcaacat gtctcgcgca cagattctgc agcaagctgg cacctccgtt ctggcacaag cgaatcaagt tccgcaaaat gtcctgtcac tgctgcgttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for SatyA.18724.a.B13.PW37521
PURIFICATION DATe: 7/2/2014
CONCENTRATION: 27mg/ml
OBSERVED MW: 43kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP GMAQVINTNS LSLLTQNNLN KSQSALGTAI ERLSSGLRIN SAKDDAAGQA IANRFTANIK GLTQASRNAN DGISIAQTTE GALNEINNNL QRVRELAVQS ANSTNSQSDL DSIQAEITQR LNEIDRVSGQ TQFNGVKVLA QDNTLTIQVG ANDGETIDID LKQINSQTLG LDTLNVQQKY DVSDTAANST EAKAALTRAG VTGDASVVKM SYTDNNGKTI DGGLAVKVGD DYYSATQNKD GSISINTTKY TADDGTSKTA LNKLGGADGK TEVVSIGGKT YAAKMAEGHN FKAQPDLAEA AATTTENPLQ KIDAALAQVD TLRSDLGAVQ NRFDSAIENL GNTVNNLTSA RSRIEDSDTA TEVSNMSRAQ ILQQAGTSVL AQANQVPQNV LSLLR
Validated NT Sequence
atggctcacc accaccacca ccatatgccc gggatggcac aagtcatcaa caccaactcg ctgagcctgc tgacgcaaaa caacctgaat aagtcgcaat cggcactggg cacggcaatc gaacgtctga gctctggcct gcgcatcaac tccgcaaagg atgacgcagc cggtcaggcc attgcaaatc gttttacggc caacatcaaa ggtctgaccc aggcctcacg caatgcaaac gatggcattt cgatcgctca gaccacggaa ggtgcgctga acgaaatcaa caataacctg caacgtgttc gcgaactggc tgtccagagc gcgaattcta ccaacagtca atccgatctg gactcaattc aagccgaaat cacgcagcgt ctgaatgaaa ttgatcgcgt gtcgggccag acccaattca atggtgttaa agtcctggcc caagataaca cgctgaccat tcaggttggc gcaaatgacg gtgaaaccat tgatatcgac ctgaaacaaa tcaacagtca gacgctgggc ctggataccc tgaatgtgca gcaaaagtat gatgttagtg acacggcagc taactccacc gaagccaaag cagcactgac gcgtgccggt gtgaccggtg atgcaagcgt ggttaaaatg tcttacacgg ataataacgg caagaccatt gacggcggtc tggccgtgaa agttggtgat gactattaca gcgcaaccca gaataaggat ggctcaattt cgatcaacac cacgaaatat accgcggatg acggtacgag caaaaccgcc ctgaataagc tgggcggtgc agatggcaaa acggaagtcg tgtctatcgg cggtaaaacc tacgcagcta agatggcaga aggtcataac tttaaggcac agccggatct ggcagaagca gcagcaacca cgaccgaaaa tccgctgcag aaaattgacg ctgcgctggc gcaagtcgat accctgcgta gcgacctggg tgctgtgcag aaccgcttcg attctgcgat tgaaaatctg ggcaacacgg ttaataacct gaccagtgct cgttcccgca tcgaagattc tgacacggcg accgaagttt caaatatgtc gcgtgctcag attctgcagc aagcgggcac cagtgtcctg gctcaggcga atcaagtccc gcagaacgtg ctgtccctgc tgcgctaagt gagtaagat
Expressed Protein Sequence
MAHHHHHHMA QVINTNSLSL LTQNNLNKSQ SALGTAIERL SSGLRINSAK DDAAGQAIAN RFTANIKGLT QASRNANDGI SIAQTTEGAL NEINNNLQRV RELAVQSANS TNSQSDLDSI QAEITQRLNE IDRVSGQTQF NGVKVLAQDN TLTIQVGAND GETIDIDLKQ INSQTLGLDT LNVQQKYDVS DTAANSTEAK AALTRAGVTG DASVVKMSYT DNNGKTIDGG LAVKVGDDYY SATQNKDGSI SINTTKYTAD DGTSKTALNK LGGADGKTEV VSIGGKTYAA KMAEGHNFKA QPDLAEAAAT TTENPLQKID AALAQVDTLR SDLGAVQNRF DSAIENLGNT VNNLTSARSR IEDSDTATEV SNMSRAQILQ QAGTSVLAQA NQVPQNVLSL LR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCG GGATGGCACA AGTCATCAAC ACCAACTCGC TGAGCCTGCT GACGCAAAAC AACCTGAATA AGTCGCAATC GGCACTGGGC ACGGCAATCG AACGTCTGAG CTCTGGCCTG CGCATCAACT CCGCAAAGGA TGACGCAGCC GGTCAGGCCA TTGCAAATCG TTTTACGGCC AACATCAAAG GTCTGACCCA GGCCTCACGC AATGCAAACG ATGGCATTTC GATCGCTCAG ACCACGGAAG GTGCGCTGAA CGAAATCAAC AATAACCTGC AACGTGTTCG CGAACTGGCT GTCCAGAGCG CGAATTCTAC CAACAGTCAA TCCGATCTGG ACTCAATTCA AGCCGAAATC ACGCAGCGTC TGAATGAAAT TGATCGCGTG TCGGGCCAGA CCCAATTCAA TGGTGTTAAA GTCCTGGCCC AAGATAACAC GCTGACCATT CAGGTTGGCG CAAATGACGG TGAAACCATT GATATCGACC TGAAACAAAT CAACAGTCAG ACGCTGGGCC TGGATACCCT GAATGTGCAG CAAAAGTATG ATGTTAGTGA CACGGCAGCT AACTCCACCG AAGCCAAAGC AGCACTGACG CGTGCCGGTG TGACCGGTGA TGCAAGCGTG GTTAAAATGT CTTACACGGA TAATAACGGC AAGACCATTG ACGGCGGTCT GGCCGTGAAA GTTGGTGATG ACTATTACAG CGCAACCCAG AATAAGGATG GCTCAATTTC GATCAACACC ACGAAATATA CCGCGGATGA CGGTACGAGC AAAACCGCCC TGAATAAGCT GGGCGGTGCA GATGGCAAAA CGGAAGTCGT GTCTATCGGC GGTAAAACCT ACGCAGCTAA GATGGCAGAA GGTCATAACT TTAAGGCACA GCCGGATCTG GCAGAAGCAG CAGCAACCAC GACCGAAAAT CCGCTGCAGA AAATTGACGC TGCGCTGGCG CAAGTCGATA CCCTGCGTAG CGACCTGGGT GCTGTGCAGA ACCGCTTCGA TTCTGCGATT GAAAATCTGG GCAACACGGT TAATAACCTG ACCAGTGCTC GTTCCCGCAT CGAAGATTCT GACACGGCGA CCGAAGTTTC AAATATGTCG CGTGCTCAGA TTCTGCAGCA AGCGGGCACC AGTGTCCTGG CTCAGGCGAA TCAAGTCCCG CAGAACGTGC TGTCCCTGCT GCGCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT