SaspA.20306.a

AaceriAGR327Cp (agglutinin-like sequence) (AIs)

CENTER ID: SaspA.20306.a
ORGANISM: Saccharomycetaceae sp. 'Ashbya aceri'
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP:
SELECT AGENT: False
NIH PRIORITY
pathogens category:

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
SaspA.20306.a.B21.GE43753 ALS3-like domain 20 318
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: R9XCR0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKYIALLLIM LSCCIAADTI SDIAFSGLNI EAVAGSQRYP HQGWAAKFDY HIKDASKIKT SDYFIVTMPS VYKIKFDNKQ NQFNVGLDGK DIFKCYALQQ AAYIHEDTVL KCEALGDMSG YKKIDGTISL TLVFSDGGSI FAHEYKNAAR FHVGFNELSF NGELKGNFEA ASVPHTDGFY YSGKTSTYNS LELYYLGFNC PTGYVLGANH RIEYDKSHPI DCSKLQVFKS KKFNDWLLPL DYGTLEEPIE CSGDMVKISH GPLEPGYRVW ANVLQALDAE TVTISNTIHF DYTCTDTNAG TTYTTTTQHT PVVVITEGMF TGTASEPPVA PHTIVTKTIC TQCSTSKTSS PSSSHKPSYV TLTKTECTVC SGETPTVSSR VQTSKSSNIA TKQTGSYPSY ASGMSSSTRS TVKSASTISN TQTSMTAFSS STKSSTKTTY GTAVTKPFSP AVSPSSELGS MKTSSTSNAQ PSSPSGLASS DYSSMKTSIS SAARPSSIAC PSSSSQSDNK STSATAVTNT SVPVTSTGGS EPPTYAISNT QPHPTLTTQR ISTIDPRSTN TQSLSTHSPS TVTVLTTSCD GGCSSQPSTS ARFISASENG TISTKNSSSA TVKTMDSGTN SFSLTVPPFS ISSNQVTSMQ TSVVTSSVLR NSTLTRTVQS CNPDCSPVSS TPTSMSSQEF VSASSTIPST LPTTPAESLV PSQPSGISHA LPSISSCTSA KIISSPTASS AIRYPEEAGF STSSATPIPS TSISQDISPS LESREGSCGS TTTFTTPHEQ HTGDGPTTSE SASSAAIASL PKPTQQPQSS VDRSSSAQPD TSSITPQGST NIVPCPLGSS SQSSIPNISS QATEYMATTP GPRTIAPKHS SGLSSAHSPT SQGTVVFSSL RATEVPGGLS TPNASTSMDW LSPSTSIKAT ASISIFRGEA AGEQFGSLSK LVLLVVPYLL L
NT Sequence
atgaagtata ttgctttact tctcatcatg ctgtcttgct gtatcgcagc agacaccatt tcagacatag ccttctctgg actcaatatc gaagcggtgg caggttccca gagatatccg catcagggat gggctgccaa atttgattat catattaagg acgcaagcaa aataaagacc tcagattatt tcattgttac gatgccaagc gtttacaaaa ttaaattcga caataagcaa aatcaattca atgttgggct tgacggcaag gatatcttca aatgctatgc tttgcagcag gctgcatata tccacgaaga tactgtgtta aaatgtgaag ctcttgggga catgtcaggc tataaaaaga ttgacggtac tatttccctg acacttgttt tcagtgatgg tggttctatc tttgctcacg aatacaagaa tgctgcaaga tttcatgtag gctttaatga attgagcttc aatggtgaat taaaagggaa ctttgaggct gctagtgtcc cgcatacaga tggtttctac tattccggga aaactagtac gtacaactcc ttggaattgt attatctagg tttcaattgc ccaaccggat atgttttggg tgccaaccac agaattgaat acgataagtc gcaccctatt gattgtagta aactgcaggt gttcaaatcc aaaaaattca atgattggct gctaccactt gattatggaa ctctagaaga accaattgaa tgctctgggg atatggttaa aatatctcat ggcccactag aacctggata ccgtgtttgg gcaaacgttt tacaagcctt agatgcagaa actgtcacca tcagcaatac gattcacttc gattatacct gtacagatac caatgcgggc acaacttaca cgacgaccac ccaacatact cctgtagttg tgataactga gggaatgttc actggtactg ctagcgaacc ccccgttgcc cctcatacaa tagtgaccaa aactatctgc acacagtgct ccacatcgaa gacttccagt ccctctagca gtcacaagcc ctcatatgta acattgacca agacagaatg cactgtctgt tccggagaaa cacctaccgt ttctagccgg gtacagacgt caaaatcgtc gaatattgcg acgaaacaga ccggcagtta tccttcatat gcgagtggta tgtctagcag tactagatca accgtcaaga gtgcttcaac tatatctaat actcagactt caatgacggc cttttctagc tctaccaagt cgagcaccaa gaccacgtac ggtactgcgg tcaccaagcc ttttagtccc gcggtttctc ccagcagcga gttgggtagt atgaagactt ccagtacatc gaatgcccag ccttcgagtc cgtccggttt ggccagcagc gactatagca gtatgaagac ttccatttca tcggctgccc ggccttcgag catagcatgc ccttcaagca gctcccagtc tgataataag agcacgtctg ctaccgcagt tacaaacact tcggtaccgg tgacttcaac tggcggcagt gagccgccaa cttacgctat ttcaaatacg caaccacatc ctactttgac cacacaaaga atatcaacaa ttgacccgcg ctccacgaat actcagtcac tgtccacgca ttcgcctagc acagttacgg ttttgactac gagttgtgat ggaggctgct cttcacagcc ttctacatct gccaggttta tcagtgcctc agaaaatggg actatcagca ccaaaaacag ctccagcgcg actgttaaaa ctatggactc cggcactaat tctttcagct tgactgtgcc gccattttct atctcaagca atcaagttac gtcaatgcaa acttctgtcg tcacctccag cgtattgagg aattcaactc taactcgaac ggtacagtca tgcaatcctg actgcagccc agtctcctca actccgacta gcatgtcgtc acaggaattt gttagcgcga gttccacgat cccatcaaca ctccccacca caccggctga gagtttagtt ccttcgcaac caagtggcat tagccacgcc ttaccttcga tttccagttg tactagcgcc aaaattatct cttcgcctac ggcatctagt gctattaggt acccggagga agcggggttc agcacttcta gcgcaacacc tataccatcg acttcgatat cacaggatat tagcccatca ctagaatcca gagaaggtag ttgcggtagt actaccacat ttaccacgcc ccatgagcaa cacactggcg atggcccaac tacttcagaa tccgcgagct ctgcagcgat cgcttcattg cctaaaccca cacaacagcc tcagagctct gttgaccgca gctcatcggc ccaaccagac acttcaagca taacccccca gggtagcacg aatattgtcc catgtcctct aggcagcagc tctcaaagct ctattccgaa tatcagcagt caagcgacgg aatatatggc tactacccca ggacccagaa ctatcgcccc taaacattcg agcggattga gctctgccca ttcaccaact tcccaaggca cagtcgtctt ttcttcgttg cgtgccaccg aggttcccgg tggtttgtcg acacccaacg caagtacatc tatggattgg ctctctccat caacttctat taaagcaaca gcctcaataa gcattttccg gggcgaggca gctggtgaac aatttgggtc cctttccaaa ctagtcctgc tcgttgtccc atatctcttg ctataa
Details for SaspA.20306.a.B21.GE43753
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 89
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI SDIAFSGLNI EAVAGSQRYP HQGWAAKFDY HIKDASKIKT SDYFIVTMPS VYKIKFDNKQ NQFNVGLDGK DIFKCYALQQ AAYIHEDTVL KCEALGDMSG YKKIDGTISL TLVFSDGGSI FAHEYKNAAR FHVGFNELSF NGELKGNFEA ASVPHTDGFY YSGKTSTYNS LELYYLGFNC PTGYVLGANH RIEYDKSHPI DCSKLQXFKS KKFNDWLLPL DYGTLXEPIE XSGDMVKXSH GPLEPGYRVW ANVLQALDAE TVXHFEXDSF LITLARILTR XYVYHYYXTX RXX
Validated NT Sequence
gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnantatacg actcnctata gggagaccac aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata ccatggctca ccaccaccac caccatatga ttagcgatat tgctttttct gggttgaata ttgaggcggt ggcaggaagc cagcgttatc cacatcaggg gtgggctgcg aagttcgact accatattaa agacgcatca aagatcaaaa cctcggatta tttcattgtc actatgcctt ccgtctacaa gatcaagttc gataacaaac agaaccagtt caacgttgga cttgatggca aagacatttt caaatgctat gcgcttcagc aggcagctta tatccacgaa gatactgtgt tgaaatgcga ggcgcttggg gatatgagtg gatacaaaaa gattgatggt acgatctcat tgacattagt attctcagat ggggggtcga tttttgccca tgaatataaa aacgccgccc gcttccatgt tgggttcaac gaactgagtt tcaatggaga attaaagggg aactttgagg ctgcttctgt ccctcatact gatggctttt attactccgg taaaacatcc acatacaata gcttggagct gtactatctt ggcttcaatt gccccaccgg gtatgttctg ggtgcgaatc atcgtattga atatgacaag agccacccta tcgactgctc gaagctgcag gnttttaaga gcaaaaagtt taatgattgg ctgcttccac ttgattacgg cacactggan gagccaatcg aatgntcggg tgatatggtc aaganttcac atggacctct ggagcccggt taccgcgttt gggctaacgt tttacaggcg ctggatgccg agacggtcan ccatttcgaa nacgattcnt ttttgattac acttgcacgg atactaacgc gggnctacgt ataccactac tacncaacnc nncgccnntn gtagtaatnn canaanggtt antgaagnag annngatccn gctgnntnac aaagnccna
Expected Protein Sequence
MAHHHHHHIS DIAFSGLNIE AVAGSQRYPH QGWAAKFDYH IKDASKIKTS DYFIVTMPSV YKIKFDNKQN QFNVGLDGKD IFKCYALQQA AYIHEDTVLK CEALGDMSGY KKIDGTISLT LVFSDGGSIF AHEYKNAARF HVGFNELSFN GELKGNFEAA SVPHTDGFYY SGKTSTYNSL ELYYLGFNCP TGYVLGANHR IEYDKSHPID CSKLQVFKSK KFNDWLLPLD YGTLEEPIEC SGDMVKISHG PLEPGYRVWA NVLQALDAET VTISNTIHFD YTCTDTNAGT TYTTTTQHTP VVVITEG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattagcg atattgcttt ttctgggttg aatattgagg cggtggcagg aagccagcgt tatccacatc aggggtgggc tgcgaagttc gactaccata ttaaagacgc atcaaagatc aaaacctcgg attatttcat tgtcactatg ccttccgtct acaagatcaa gttcgataac aaacagaacc agttcaacgt tggacttgat ggcaaagaca ttttcaaatg ctatgcgctt cagcaggcag cttatatcca cgaagatact gtgttgaaat gcgaggcgct tggggatatg agtggataca aaaagattga tggtacgatc tcattgacat tagtattctc agatgggggg tcgatttttg cccatgaata taaaaacgcc gcccgcttcc atgttgggtt caacgaactg agtttcaatg gagaattaaa ggggaacttt gaggctgctt ctgtccctca tactgatggc ttttattact ccggtaaaac atccacatac aatagcttgg agctgtacta tcttggcttc aattgcccca ccgggtatgt tctgggtgcg aatcatcgta ttgaatatga caagagccac cctatcgact gctcgaagct gcaggttttt aagagcaaaa agtttaatga ttggctgctt ccacttgatt acggcacact ggaggagcca atcgaatgtt cgggtgatat ggtcaagatt tcacatggac ctctggagcc cggttaccgc gtttgggcta acgttttaca ggcgctggat gccgagacgg tcaccatttc gaacacgatt cattttgatt acacttgcac ggatactaac gcggggacta cgtataccac tactacacaa cacacgcctg ttgtagtaat cacagaaggg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat