SSGCID: Target Page

SascA.19500.j

Group 3 allergen SMIPP-S Yv5004E08

CENTER ID:	SascA.19500.j
ORGANISM:	Sarcoptes scabiei
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	1
SELECT AGENT:	False
NIH PRIORITY pathogens category:	I

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
SascA.19500.j.B1.GE41302	full length		1	256

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	VectorBase:SSCA007249
UniProt:	Q6VPU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFVLRYRIVW FVAVISLTIL CSNISYAIRG GAKTNIEQVP WTVAVFTYPG EESLICGGAI LSEWFVLTAV QCVYDQKPDT IVVQYDTSNL LLERGKSDSY VSKVHLLRYR NETMDNDIAI LEFSRPLQLD QVKSKAAKLP SVEFDPMTGS EVLVSGYGDG QTMDPNDFQL NSANLTVISR EECQKKYTQK YISLQVFCAI KQDVYLESGD AGDPAVQNDT LVGVAVYFKD RAGGVPEVFT KVGSFVSWIE KIIKRK

NT Sequence

atgtttgttc tacgctatcg aattgtttgg tttgttgcag tgattagttt aacgattcta tgcagcaata tatcttacgc aatcagagga ggggcaaaaa ctaacatcga gcaagtgcca tggactgtcg ctgtttttac gtacccaggt gaagaatcat tgatttgtgg tggggccatt ctaagtgaat ggtttgtttt gactgctgtt caatgtgttt atgatcagaa accagacact attgtggtcc aatatgacac tagtaatcta ttgctcgaac gtggaaagtc tgattcatat gttagtaaag tgcatctatt gcgttatcgc aacgagacta tggataacga tattgctatt ctcgaatttt cgagaccatt gcagttggat caagtcaaaa gtaaagctgc taaactgcct agtgttgaat ttgatccaat gactggaagt gaagtgttgg tttctggcta tggagatggt caaacgatgg atccgaatga tttccaattg aattccgcta atctcacagt gataagcaga gaagaatgtc aaaaaaaata tactcaaaaa tatataagtt tgcaagtatt ttgtgcgata aaacaagatg tttatcttga gagtggtgat gcaggtgatc cggctgttca aaatgatacc ttagttggtg ttgctgtata ttttaaagat cgagccggag gagttccaga agttttcact aaagtcggat catttgtctc gtggatagaa aaaataataa agaggaaatg a

Details for SascA.19500.j.B1.GE41302

HARVESTED ON:	3/8/2017
SEQUENCED ON:	3/9/2017
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMF VLRYRIVWFV AVISLTILCS NISYAIRGGA KTNIEQVPWT VAVFTYPGEE SLICGGAILS EWFVLTAVQC VYDQKPDTIV VQYDTSNLLL ERGKSDSYVS KVHLLRYRNE TMDNDIAILE FSRPLQLDQV KSKAAKLPSV EFDPMTGSEV LVSGYGDGQT MDPNDFQLNS ANLTVISREE CQKKYTQKYI SLQVFCAIKQ DVYLESGDAG DPAVQNDTLV GVAVYFKDRA GGVPEVFTKV GSFVSWIEXI IKRK

Validated NT Sequence

atggctcacc accaccacca ccatatgttt gttctacgct atcgaattgt ttggtttgtt gcagtgatta gtttaacgat tctatgcagc aatatatctt acgcaatcag aggaggggca aaaactaaca tcgagcaagt gccatggact gtcgctgttt ttacgtaccc aggtgaagaa tcattgattt gtggtggggc cattctaagt gaatggtttg ttttgactgc tgttcaatgt gtttatgatc agaaaccaga cactattgtg gtccaatatg acactagtaa tctattgctc gaacgtggaa agtctgattc atatgttagt aaagtgcatc tattgcgtta tcgcaacgag actatggata acgatattgc tattctcgaa ttttcgagac cattgcagtt ggatcaagtc aaaagtaaag ctgctaaact gcctagtgtt gaatttgatc caatgactgg aagtgaagtg ttggtttctg gctatggaga tggtcaaacg atggatccga atgatttcca attgaattcc gctaatctca cagtgataag cagagaagaa tgtcaaaaaa aatatactca aaaatatata agtttgcaag tattttgtgc gataaaacaa gatgtttatc ttgagagtgg tgatgcaggt gatccggctg ttcaaaatga taccttagtt ggtgttgctg tatattttaa agatcgagcc ggaggagttc cagaagtttt cactaaagtc ggatcatttg tctcgtggat agaaannata ataaagagga aataagtgag taagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttg ttctacgcta tcgaattgtt tggtttgttg cagtgattag tttaacgatt ctatgcagca atatatctta cgcaatcaga ggaggggcaa aaactaacat cgagcaagtg ccatggactg tcgctgtttt tacgtaccca ggtgaagaat cattgatttg tggtggggcc attctaagtg aatggtttgt tttgactgct gttcaatgtg tttatgatca gaaaccagac actattgtgg tccaatatga cactagtaat ctattgctcg aacgtggaaa gtctgattca tatgttagta aagtgcatct attgcgttat cgcaacgaga ctatggataa cgatattgct attctcgaat tttcgagacc attgcagttg gatcaagtca aaagtaaagc tgctaaactg cctagtgttg aatttgatcc aatgactgga agtgaagtgt tggtttctgg ctatggagat ggtcaaacga tggatccgaa tgatttccaa ttgaattccg ctaatctcac agtgataagc agagaagaat gtcaaaaaaa atatactcaa aaatatataa gtttgcaagt attttgtgcg ataaaacaag atgtttatct tgagagtggt gatgcaggtg atccggctgt tcaaaatgat accttagttg gtgttgctgt atattttaaa gatcgagccg gaggagttcc agaagttttc actaaagtcg gatcatttgt ctcgtggata gaaaaaataa taaagaggaa atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat