SaceA.19172.a
Pantothenate kinase (CAB1) (PanK)
| CENTER ID: | SaceA.19172.a |
| ORGANISM: | Saccharomyces cerevisiae |
| ASSOCIATED DISEASE: | Systemic diseases |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | unclassified |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
unclassified |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| SaceA.19172.a.B11.GE42560 | full length | 1 | 367 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| SaceA.19172.a.B11.PW38534 | full length | 1 | 367 |
Structures
6UJ5
DEPOSITED: 10/2/2019
DETERMINATION: XRay
CLONE: SaceA.19172.a.B11.GE42560
PROTEIN: SaceA.19172.a.B11.PW38534
Publications by SSGCID
High-resolution crystal structure and chemical screening reveal pantothenate kinase as a new target for antifungal development
Fox D, Staker BL, Ojo KK, Gihaz S, Gareiss P, Choi JY, Pal AC, Renard I, Surovsteva Y, Thekkiniath J, Plummer M, Fuller K, Ben Mamoun C
Structure - 2022
volume 30, issue 11, pages 1494-1507
PDB:
6UJ5
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | FungiDB:YDR531W |
| UniProt: | Q04430 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPRITQEISY NCDYGDNTFN LAIDIGGTLA KVVFSPIHSN RLMFYTIETE KIDKFMELLH SIIKEHNNGC YRMTHIIATG GGAFKFYDLL YENFPQIKGI SRFEEMEGLI HGLDFFIHEI PDEVFTYNDQ DGERIIPTSS GTMDSKAIYP YLLVNIGSGV SILKVTEPNN FSRVGGSSLG GGTLWGLLSL ITGAQTYDQM LDWAQEGDNS SVDMLVGDIY GTDYNKIGLK SSAIASSFGK VFQNRMTSNK SLENNENKLY SSHESIEKNN GQMFKNPDIC KSLLFAISNN IGQIAYLQAK INNIQNIYFG GSYTRGHLTT MNTLSYAINF WSQGSKQAFF LKHEGYLGAM GAFLSASRHS STKKTST
NT Sequence
atgccgcgaa ttactcaaga gatatcttac aattgcgatt atggcgacaa tactttcaac cttgctattg atataggagg cactctggct aaagtagtct tctcgcctat acacagtaac aggctgatgt tctacaccat tgaaacagag aaaattgaca agttcatgga acttctgcat tctattatca aagaacataa caatggatgc tatagaatga ctcatataat tgccactggt ggtggcgcct tcaagtttta tgatttgttg tatgaaaatt ttcctcaaat aaaaggtata tcgaggttcg aagaaatgga aggcttaatt cacggtttag actttttcat tcatgagatt cccgatgaag ttttcactta caacgatcaa gatggcgaaa ggataatacc caccagttcc ggcaccatgg actcaaaggc tatctaccca taccttctag tcaatatagg gtcgggtgtc tcaatattaa aagtcaccga accaaacaat tttagtagag taggcggttc ttcactggga ggaggaactc tttggggcct attatcacta attactggcg ctcaaactta tgatcagatg ctcgattggg cacaagaggg tgacaattct agcgttgata tgctagttgg agatatttat ggaacagact ataataaaat tggtctaaag tcgtcagcta ttgcaagttc atttggtaaa gttttccaaa atagaatgac atctaacaaa tctttggaaa acaacgaaaa taaattatat tcctcacatg agtctattga gaaaaacaat ggacaaatgt ttaagaatcc tgatatttgt aaaagtcttc tattcgccat ctccaacaat attgggcaaa tagcttattt gcaagctaaa atcaataata tacagaatat atactttggc ggatcttata ccagaggaca tttgactacc atgaacactt tgagctacgc tattaatttt tggtcacaag gatcaaagca agcgtttttt ctcaaacatg aaggctattt gggtgcaatg ggtgctttcc taagcgcgtc tcgtcattca tctactaaga aaacaagtac gtag
Details for SaceA.19172.a.B11.GE42560
| HARVESTED ON: | 7/3/2018 |
| SEQUENCED ON: | 7/10/2018 |
| EXPECTED MW: | 42kDa |
| OBSERVED MW: | 40kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMP RITQEISYNC DYGDNTFNLA IDIGGTLAKV VFSPIHSNRL MFYTIETEKI DKFMELLHSI IKEHNNGCYR MTHIIATGGG AFKFYDLLYE NFPQIKGISR FEEMEGLIHG LDFFIHEIPD EVFTYNDQDG ERIIPTSSGT MDSKAIYPYL LVNIGSGVSI LKVTEPNNFS RVGGSSLGGG TLWGLLSLIT GAQTYDQMLD WAQEGDNSSV DMLVGDIYGT DYNKIGLKSS AIASSFGKVF QNRMTSNKSL ENNENKLYSS HESIEKNNGQ MFKNPDICKS LLFAISNNIG QIAYLQAKIN NIQNIYFGGS YTRGHLTTMN TLSYAINFWS QGSKQAFFLK HEGXXGAMGA FLSAS
Validated NT Sequence
accatggctc accaccacca ccaccatatg ccgcgcatta cccaagagat cagctataac tgcgactacg gcgataatac ctttaacctg gcgattgaca tcggtggcac cctggcgaag gtggttttca gcccgatcca cagcaaccgt ctgatgtttt acaccattga aaccgaaaag atcgacaaat tcatggagct gctgcacagc atcattaaag aacacaacaa cggttgctat cgtatgaccc acatcattgc gaccggtggc ggtgcgttca agttttacga tctgctgtat gagaacttcc cgcagatcaa aggcattagc cgttttgagg aaatggaagg tctgattcac ggcctggact tctttatcca cgagattccg gatgaagtgt tcacctacaa cgaccaagat ggcgagcgta tcattccgac cagcagcggt acgatggaca gcaaggcgat ctacccgtat ctgctggtga acattggtag cggcgtgagc atcctgaaag ttaccgaacc gaacaacttt agccgtgttg gcggtagcag cctgggcggt ggcaccctgt ggggtctgct gagcctgatt accggcgcgc agacctacga ccaaatgctg gattgggcgc aggaaggtga caacagcagc gtggatatgc tggttggtga catctacggc accgattata acaagattgg tctgaaaagc agcgcgatcg cgagcagctt cggcaaggtt tttcagaacc gtatgaccag caacaagagc ctggagaaca acgaaaacaa actgtacagc agccacgaga gcattgagaa gaacaacggt caaatgttca agaacccgga tatttgcaaa agcctgctgt ttgcgatcag caacaacatc ggccagattg cgtatctgca agcgaagatc aacaacattc aaaacatcta cttcggtggc agctataccc gtggtcacct gaccaccatg aacaccctga gctacgcgat caacttttgg agccagggta gcaagcaagc gttctttctg aaacacgaag gctannnngg cgcgatgggc gcgtttctga gcgcgagcc
Expected Protein Sequence
MAHHHHHHMP RITQEISYNC DYGDNTFNLA IDIGGTLAKV VFSPIHSNRL MFYTIETEKI DKFMELLHSI IKEHNNGCYR MTHIIATGGG AFKFYDLLYE NFPQIKGISR FEEMEGLIHG LDFFIHEIPD EVFTYNDQDG ERIIPTSSGT MDSKAIYPYL LVNIGSGVSI LKVTEPNNFS RVGGSSLGGG TLWGLLSLIT GAQTYDQMLD WAQEGDNSSV DMLVGDIYGT DYNKIGLKSS AIASSFGKVF QNRMTSNKSL ENNENKLYSS HESIEKNNGQ MFKNPDICKS LLFAISNNIG QIAYLQAKIN NIQNIYFGGS YTRGHLTTMN TLSYAINFWS QGSKQAFFLK HEGYLGAMGA FLSASRHSST KKTST
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc gcattaccca agagatcagc tataactgcg actacggcga taataccttt aacctggcga ttgacatcgg tggcaccctg gcgaaggtgg ttttcagccc gatccacagc aaccgtctga tgttttacac cattgaaacc gaaaagatcg acaaattcat ggagctgctg cacagcatca ttaaagaaca caacaacggt tgctatcgta tgacccacat cattgcgacc ggtggcggtg cgttcaagtt ttacgatctg ctgtatgaga acttcccgca gatcaaaggc attagccgtt ttgaggaaat ggaaggtctg attcacggcc tggacttctt tatccacgag attccggatg aagtgttcac ctacaacgac caagatggcg agcgtatcat tccgaccagc agcggtacga tggacagcaa ggcgatctac ccgtatctgc tggtgaacat tggtagcggc gtgagcatcc tgaaagttac cgaaccgaac aactttagcc gtgttggcgg tagcagcctg ggcggtggca ccctgtgggg tctgctgagc ctgattaccg gcgcgcagac ctacgaccaa atgctggatt gggcgcagga aggtgacaac agcagcgtgg atatgctggt tggtgacatc tacggcaccg attataacaa gattggtctg aaaagcagcg cgatcgcgag cagcttcggc aaggtttttc agaaccgtat gaccagcaac aagagcctgg agaacaacga aaacaaactg tacagcagcc acgagagcat tgagaagaac aacggtcaaa tgttcaagaa cccggatatt tgcaaaagcc tgctgtttgc gatcagcaac aacatcggcc agattgcgta tctgcaagcg aagatcaaca acattcaaaa catctacttc ggtggcagct atacccgtgg tcacctgacc accatgaaca ccctgagcta cgcgatcaac ttttggagcc agggtagcaa gcaagcgttc tttctgaaac acgaaggcta tctgggcgcg atgggcgcgt ttctgagcgc gagccgtcat agcagcacca aaaagaccag cacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for SaceA.19172.a.B11.PW38534
| PURIFICATION DATe: | 10/1/2018 |
| CONCENTRATION: | 29.49mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 16 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP RITQEISYNC DYGDNTFNLA IDIGGTLAKV VFSPIHSNRL MFYTIETEKI DKFMELLHSI IKEHNNGCYR MTHIIATGGG AFKFYDLLYE NFPQIKGISR FEEMEGLIHG LDFFIHEIPD EVFTYNDQDG ERIIPTSSGT MDSKAIYPYL LVNIGSGVSI LKVTEPNNFS RVGGSSLGGG TLWGLLSLIT GAQTYDQMLD WAQEGDNSSV DMLVGDIYGT DYNKIGLKSS AIASSFGKVF QNRMTSNKSL ENNENKLYSS HESIEKNNGQ MFKNPDICKS LLFAISNNIG QIAYLQAKIN NIQNIYFGGS YTRGHLTTMN TLSYAINFWS QGSKQAFFLK HEGXXGAMGA FLSAS
Validated NT Sequence
accatggctc accaccacca ccaccatatg ccgcgcatta cccaagagat cagctataac tgcgactacg gcgataatac ctttaacctg gcgattgaca tcggtggcac cctggcgaag gtggttttca gcccgatcca cagcaaccgt ctgatgtttt acaccattga aaccgaaaag atcgacaaat tcatggagct gctgcacagc atcattaaag aacacaacaa cggttgctat cgtatgaccc acatcattgc gaccggtggc ggtgcgttca agttttacga tctgctgtat gagaacttcc cgcagatcaa aggcattagc cgttttgagg aaatggaagg tctgattcac ggcctggact tctttatcca cgagattccg gatgaagtgt tcacctacaa cgaccaagat ggcgagcgta tcattccgac cagcagcggt acgatggaca gcaaggcgat ctacccgtat ctgctggtga acattggtag cggcgtgagc atcctgaaag ttaccgaacc gaacaacttt agccgtgttg gcggtagcag cctgggcggt ggcaccctgt ggggtctgct gagcctgatt accggcgcgc agacctacga ccaaatgctg gattgggcgc aggaaggtga caacagcagc gtggatatgc tggttggtga catctacggc accgattata acaagattgg tctgaaaagc agcgcgatcg cgagcagctt cggcaaggtt tttcagaacc gtatgaccag caacaagagc ctggagaaca acgaaaacaa actgtacagc agccacgaga gcattgagaa gaacaacggt caaatgttca agaacccgga tatttgcaaa agcctgctgt ttgcgatcag caacaacatc ggccagattg cgtatctgca agcgaagatc aacaacattc aaaacatcta cttcggtggc agctataccc gtggtcacct gaccaccatg aacaccctga gctacgcgat caacttttgg agccagggta gcaagcaagc gttctttctg aaacacgaag gctannnngg cgcgatgggc gcgtttctga gcgcgagcc
Expressed Protein Sequence
MAHHHHHHMP RITQEISYNC DYGDNTFNLA IDIGGTLAKV VFSPIHSNRL MFYTIETEKI DKFMELLHSI IKEHNNGCYR MTHIIATGGG AFKFYDLLYE NFPQIKGISR FEEMEGLIHG LDFFIHEIPD EVFTYNDQDG ERIIPTSSGT MDSKAIYPYL LVNIGSGVSI LKVTEPNNFS RVGGSSLGGG TLWGLLSLIT GAQTYDQMLD WAQEGDNSSV DMLVGDIYGT DYNKIGLKSS AIASSFGKVF QNRMTSNKSL ENNENKLYSS HESIEKNNGQ MFKNPDICKS LLFAISNNIG QIAYLQAKIN NIQNIYFGGS YTRGHLTTMN TLSYAINFWS QGSKQAFFLK HEGYLGAMGA FLSASRHSST KKTST
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCGC GCATTACCCA AGAGATCAGC TATAACTGCG ACTACGGCGA TAATACCTTT AACCTGGCGA TTGACATCGG TGGCACCCTG GCGAAGGTGG TTTTCAGCCC GATCCACAGC AACCGTCTGA TGTTTTACAC CATTGAAACC GAAAAGATCG ACAAATTCAT GGAGCTGCTG CACAGCATCA TTAAAGAACA CAACAACGGT TGCTATCGTA TGACCCACAT CATTGCGACC GGTGGCGGTG CGTTCAAGTT TTACGATCTG CTGTATGAGA ACTTCCCGCA GATCAAAGGC ATTAGCCGTT TTGAGGAAAT GGAAGGTCTG ATTCACGGCC TGGACTTCTT TATCCACGAG ATTCCGGATG AAGTGTTCAC CTACAACGAC CAAGATGGCG AGCGTATCAT TCCGACCAGC AGCGGTACGA TGGACAGCAA GGCGATCTAC CCGTATCTGC TGGTGAACAT TGGTAGCGGC GTGAGCATCC TGAAAGTTAC CGAACCGAAC AACTTTAGCC GTGTTGGCGG TAGCAGCCTG GGCGGTGGCA CCCTGTGGGG TCTGCTGAGC CTGATTACCG GCGCGCAGAC CTACGACCAA ATGCTGGATT GGGCGCAGGA AGGTGACAAC AGCAGCGTGG ATATGCTGGT TGGTGACATC TACGGCACCG ATTATAACAA GATTGGTCTG AAAAGCAGCG CGATCGCGAG CAGCTTCGGC AAGGTTTTTC AGAACCGTAT GACCAGCAAC AAGAGCCTGG AGAACAACGA AAACAAACTG TACAGCAGCC ACGAGAGCAT TGAGAAGAAC AACGGTCAAA TGTTCAAGAA CCCGGATATT TGCAAAAGCC TGCTGTTTGC GATCAGCAAC AACATCGGCC AGATTGCGTA TCTGCAAGCG AAGATCAACA ACATTCAAAA CATCTACTTC GGTGGCAGCT ATACCCGTGG TCACCTGACC ACCATGAACA CCCTGAGCTA CGCGATCAAC TTTTGGAGCC AGGGTAGCAA GCAAGCGTTC TTTCTGAAAC ACGAAGGCTA TCTGGGCGCG ATGGGCGCGT TTCTGAGCGC GAGCCGTCAT AGCAGCACCA AAAAGACCAG CACCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT