SSGCID: Target Page

RityA.19242.a

VirB10 protein of the type IV secretion system

CENTER ID:	RityA.19242.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.19242.a.B2.GE40095	soluble domain		57	483
RityA.19242.a.B3.GE40096	globular domain (aln H.pyl struc)		191	483

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.290
RefSeq:	WP_011190747.1
UniProt:	Q68X80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVAEQNNNNN TDSLSGTDLP EVQRELSKVS VSFNKNIAIV VVICAILIYI FYTLFFDMRK EEIPETKIPS NIVTPVTDVN DIIPEIPKLP DPPKLERPTV LQPPPPPPPV VEVPPVLPPI SIEGNKEQTL QLPPVSLPTT SSTLVESDAE KQRREAKRKS AIVLVGGVEP KKTPEQITEA VTFKDRGDMF LLLGRGKLID AVLETGINSD LGGEIRAIIS RDVFSEKGKV ILIPKGSKIF GKYATSTSSD SYGRVSIIWD RVDLTNGYTI EFDSPAVDNL GRPGLQGRVD NKYKEQFANA VLQSGFNIGL AKILDKLVPP PIDSQAAATN SATATQILNI AQTISSNTAI DVNTRIVTIC TNILAAITDK TSIAYTTMTQ ACATAQNASS ANTSEQRLQT LVQAVNTAAS NLLTTTSIAS TPTQAQQAST QAFTDVTNVV QNMITQQHFK PTTTVNQGTP IRIYVNKDYK FPRTVLLKSK VMK

NT Sequence

atggttgcag agcaaaataa caataataat accgattctt tatcaggtac agacttacct gaggtacaaa gggaattatc taaagtttcg gttagtttta ataagaatat tgcaatagta gtggtaattt gtgctattct tatatatatc ttttatactc ttttttttga catgagaaaa gaagaaatac ctgagactaa gataccgagt aatattgtaa cgcctgttac ggatgttaat gatattattc cagagatccc aaagttacca gatccaccta aacttgagag gccaacagtg cttcaaccac cgcctcctcc tccgccagta gtagaagttc cgcctgtttt acctccgatt tctatagaag ggaataaaga gcagacactt caattgccac cggtgtcgtt acctacgacc tcatcaacgt tagttgagag tgatgcagag aagcaacgcc gtgaagcaaa acggaaatca gctatagtgt tagtgggtgg tgtagagcct aaaaaaacac cagaacaaat tacagaagcg gtgacattta aagatcgtgg tgatatgttt ttgttgcttg gacgcggtaa gttaatcgat gcagtgcttg agacaggaat aaatagtgat ctcggtggtg aaataagggc aattattagt agagacgtat tttctgaaaa aggtaaagtg atattaatac caaaagggtc aaaaatattc ggtaaatatg caacttcaac ttcatcagat agttacggta gggtttctat catatgggat cgagtcgatt taactaatgg ttataccata gaatttgact cacctgcagt tgataatttg ggtagacctg gattacaggg gagggttgat aataaatata aggaacaatt tgcaaatgcc gtattacaat ctggctttaa tataggtctt gctaaaatcc tagataagtt agtcccacct cctatagatt cacaagcagc ggctaccaac agtgctacag caacacaaat attaaatata gctcaaacta tttcgtctaa tactgctatt gatgtaaata ctaggatagt tacaatttgt actaatatac ttgctgctat tactgataaa acttctattg cttatactac tatgactcaa gcatgtgcga cagcacaaaa tgcttcttca gccaatactt ctgaacagag acttcaaaca ttagtacagg cagttaatac agcagcttca aatttgctta ctactacatc tattgcctcg actcctacac aagcacaaca agcttctacg caagctttta ctgatgtgac taatgttgta caaaatatga taactcaaca gcattttaag cctacgacaa cggttaatca aggtacaccg attagaatat atgttaataa ggattataaa tttccaagaa ccgttttatt aaaatcgaag gtaatgaaat ga

Details for RityA.19242.a.B2.GE40095

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	47kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMD MRKEEIPETK IPSNIVTPVT DVNDIIPEIP KLPDPPKLER PTVLQPPPPP PPVVEVPPVL PPISIEGNKE QTLQLPPVSL PTTSSTLVES DAEKQRREAK RKSAIVLVGG VEPKKTPEQI TEAVTFKDRG DMFLLLGRGK LIDAVLETGI NSDLGGEIRA IISRDVFSEK GKVILIPKGS KIFGKYATST SSDSYGRVSI IWDRVDLTNG YTIEFDSPAV DNLGRPGLQG RVDNKYKEQF ANAVLQSGFN IGLAKILDKL VPPPIDSQAA ATNSATATQI LNIAQTISSN TAIDVNTRIV TICTNILAAI TDKTSIAYTT MTQACATAQN ASSANTSEQR LQTLVQAVNT AASNLLTTTS IASTPTQAQQ ASTQAFTDVT NVVQNMITQQ HFKPTTTVNQ GTPIRIYVNK DYKFPR

Validated NT Sequence

ggttcttgga aatttataat ccttattaac atatattcta atcggtgtac cttgattaac cgttgtcgta ggcttaaaat gctgttgagt tatcatattt tgtacaacat tagtcacatc agtaaaagct tgcgtagaag cttgttgtgc ttgtgtagga gtcgaggcaa tagatgtagt agtaagcaaa tttgaagctg ctgtattaac tgcctgtact aatgtttgaa gtctctgttc agaagtattg gctgaagaag cattttgtgc tgtcgcacat gcttgagtca tagtagtata agcaatagaa gttttatcag taatagcagc aagtatatta gtacaaattg taactatcct agtatttaca tcaatagcag tattagacga aatagtttga gctatattta atatttgtgt tgctgtagca ctgttggtag ccgctgcttg tgaatctata ggaggtggga ctaacttatc taggatttta gcaagaccta tattaaagcc agattgtaat acggcatttg caaattgttc cttatattta ttatcaaccc tcccctgtaa tccaggtcta cccaaattat caactgcagg tgagtcaaat tctatggtat aaccattagt taaatcgact cgatcccata tgatagaaac cctaccgtaa ctatctgatg aagttgaagt tgcatattta ccgaatattt ttgacccttt tggtattaat atcactttac ctttttcaga aaatacgtct ctactaataa ttgcccttat ttcaccaccg agatcactat ttattcctgt ctcaagcact gcatcgatta acttaccgcg tccaagcaac aaaaacatat caccacgatc tttaaatgtc accgcttctg taatttgttc tggtgttttt ttaggctcta caccacccac taacactata gctgatttcc gttttgcttc acggcgttgc ttctctgcat cactctcaac taacgttgat gaggtcgtag gtaacgacac cggtggcaat tgaagtgtct gctctttatt cccttctata gaaatcggag gtaaaacagg cggaacttct actactggcg gaggaggagg cggtggttga agcactgttg gcctctcaag tttaggtgga tctggtaact ttgggatctc tggaataata tcattaacat ccgtaacagg cgttacaata ttactcggta tcttagtctc aggtatttct tcttttctca tgtccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHDM RKEEIPETKI PSNIVTPVTD VNDIIPEIPK LPDPPKLERP TVLQPPPPPP PVVEVPPVLP PISIEGNKEQ TLQLPPVSLP TTSSTLVESD AEKQRREAKR KSAIVLVGGV EPKKTPEQIT EAVTFKDRGD MFLLLGRGKL IDAVLETGIN SDLGGEIRAI ISRDVFSEKG KVILIPKGSK IFGKYATSTS SDSYGRVSII WDRVDLTNGY TIEFDSPAVD NLGRPGLQGR VDNKYKEQFA NAVLQSGFNI GLAKILDKLV PPPIDSQAAA TNSATATQIL NIAQTISSNT AIDVNTRIVT ICTNILAAIT DKTSIAYTTM TQACATAQNA SSANTSEQRL QTLVQAVNTA ASNLLTTTSI ASTPTQAQQA STQAFTDVTN VVQNMITQQH FKPTTTVNQG TPIRIYVNKD YKFPRTVLLK SKVMK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacatga gaaaagaaga aatacctgag actaagatac cgagtaatat tgtaacgcct gttacggatg ttaatgatat tattccagag atcccaaagt taccagatcc acctaaactt gagaggccaa cagtgcttca accaccgcct cctcctccgc cagtagtaga agttccgcct gttttacctc cgatttctat agaagggaat aaagagcaga cacttcaatt gccaccggtg tcgttaccta cgacctcatc aacgttagtt gagagtgatg cagagaagca acgccgtgaa gcaaaacgga aatcagctat agtgttagtg ggtggtgtag agcctaaaaa aacaccagaa caaattacag aagcggtgac atttaaagat cgtggtgata tgtttttgtt gcttggacgc ggtaagttaa tcgatgcagt gcttgagaca ggaataaata gtgatctcgg tggtgaaata agggcaatta ttagtagaga cgtattttct gaaaaaggta aagtgatatt aataccaaaa gggtcaaaaa tattcggtaa atatgcaact tcaacttcat cagatagtta cggtagggtt tctatcatat gggatcgagt cgatttaact aatggttata ccatagaatt tgactcacct gcagttgata atttgggtag acctggatta caggggaggg ttgataataa atataaggaa caatttgcaa atgccgtatt acaatctggc tttaatatag gtcttgctaa aatcctagat aagttagtcc cacctcctat agattcacaa gcagcggcta ccaacagtgc tacagcaaca caaatattaa atatagctca aactatttcg tctaatactg ctattgatgt aaatactagg atagttacaa tttgtactaa tatacttgct gctattactg ataaaacttc tattgcttat actactatga ctcaagcatg tgcgacagca caaaatgctt cttcagccaa tacttctgaa cagagacttc aaacattagt acaggcagtt aatacagcag cttcaaattt gcttactact acatctattg cctcgactcc tacacaagca caacaagctt ctacgcaagc ttttactgat gtgactaatg ttgtacaaaa tatgataact caacagcatt ttaagcctac gacaacggtt aatcaaggta caccgattag aatatatgtt aataaggatt ataaatttcc aagaaccgtt ttattaaaat cgaaggtaat gaaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for RityA.19242.a.B3.GE40096

HARVESTED ON:	3/24/2016
SEQUENCED ON:	3/25/2016
EXPECTED MW:	32kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML LLGRGKLIDA VLETGINSDL GGEIRAIISR DVFSEKGKVI LIPKGSKIFG KYATSTSSDS YGRVSIIWDR VDLTNGYTIE FDSPAVDNLG RPGLQGRVDN KYKEQFANAV LQSGFNIGLA KILDKLVPPP IDSQAAATNS ATATQILNIA QTISSNTAID VNTRIVTICT NILAAITDKT SIAYTTMTQA CATAQNASSA NTSEQRLQTL VQAVNTAASN LLTTTSIAST PTQAQQASTQ AFTDVTNVVQ NMITQQHFKP TTTVNQGTPI RIYVNKDYKF PRTXXXKSKV MK

Validated NT Sequence

ttactcactt atttcattac cttcgatttt nnnnnnncgg ttcttggaaa tttataatcc ttattaacat atattctaat cggtgtacct tgattaaccg ttgtcgtagg cttaaaatgc tgttgagtta tcatattttg tacaacatta gtcacatcag taaaagcttg cgtagaagct tgttgtgctt gtgtaggagt cgaggcaata gatgtagtag taagcaaatt tgaagctgct gtattaactg cctgtactaa tgtttgaagt ctctgttcag aagtattggc tgaagaagca ttttgtgctg tcgcacatgc ttgagtcata gtagtataag caatagaagt tttatcagta atagcagcaa gtatattagt acaaattgta actatcctag tatttacatc aatagcagta ttagacgaaa tagtttgagc tatatttaat atttgtgttg ctgtagcact gttggtagcc gctgcttgtg aatctatagg aggtgggact aacttatcta ggattttagc aagacctata ttaaagccag attgtaatac ggcatttgca aattgttcct tatatttatt atcaaccctc ccctgtaatc caggtctacc caaattatca actgcaggtg agtcaaattc tatggtataa ccattagtta aatcgactcg atcccatatg atagaaaccc taccgtaact atctgatgaa gttgaagttg catatttacc gaatattttt gacccttttg gtattaatat cactttacct ttttcagaaa atacgtctct actaataatt gcccttattt caccaccgag atcactattt attcctgtct caagcactgc atcgattaac ttaccgcgtc caagcaacaa catatggtgg tggtggtggt gagccat

Expected Protein Sequence

MAHHHHHHLL LGRGKLIDAV LETGINSDLG GEIRAIISRD VFSEKGKVIL IPKGSKIFGK YATSTSSDSY GRVSIIWDRV DLTNGYTIEF DSPAVDNLGR PGLQGRVDNK YKEQFANAVL QSGFNIGLAK ILDKLVPPPI DSQAAATNSA TATQILNIAQ TISSNTAIDV NTRIVTICTN ILAAITDKTS IAYTTMTQAC ATAQNASSAN TSEQRLQTLV QAVNTAASNL LTTTSIASTP TQAQQASTQA FTDVTNVVQN MITQQHFKPT TTVNQGTPIR IYVNKDYKFP RTVLLKSKVM K

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgttgc ttggacgcgg taagttaatc gatgcagtgc ttgagacagg aataaatagt gatctcggtg gtgaaataag ggcaattatt agtagagacg tattttctga aaaaggtaaa gtgatattaa taccaaaagg gtcaaaaata ttcggtaaat atgcaacttc aacttcatca gatagttacg gtagggtttc tatcatatgg gatcgagtcg atttaactaa tggttatacc atagaatttg actcacctgc agttgataat ttgggtagac ctggattaca ggggagggtt gataataaat ataaggaaca atttgcaaat gccgtattac aatctggctt taatataggt cttgctaaaa tcctagataa gttagtccca cctcctatag attcacaagc agcggctacc aacagtgcta cagcaacaca aatattaaat atagctcaaa ctatttcgtc taatactgct attgatgtaa atactaggat agttacaatt tgtactaata tacttgctgc tattactgat aaaacttcta ttgcttatac tactatgact caagcatgtg cgacagcaca aaatgcttct tcagccaata cttctgaaca gagacttcaa acattagtac aggcagttaa tacagcagct tcaaatttgc ttactactac atctattgcc tcgactccta cacaagcaca acaagcttct acgcaagctt ttactgatgt gactaatgtt gtacaaaata tgataactca acagcatttt aagcctacga caacggttaa tcaaggtaca ccgattagaa tatatgttaa taaggattat aaatttccaa gaaccgtttt attaaaatcg aaggtaatga aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat