RityA.18707.a

RARP-2; putative uncharacterized protein

CENTER ID: RityA.18707.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.18707.a.B11.GE38035 full length 1 612
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.621
RefSeq: YP_067547.1
UniProt: Q68WC7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQKILLQGYD INMNNTNPDI KEMRTLNDLP SAKACEYFIY AHGSEFFYQN SHLGLLGRIQ LYFMPDPSYL SVSILSDEGV TSMLDITLLN KIQRNTPVNQ CNIVHIFSCH SGAAQHHLNF IEGNIVLCTY NKASDSNVIQ LAKSNYETRT KNHSSLIEYI HDNFHLLVAS DFSISYKLDN KIYNFSFSAD KIKNMKSIEE LPSFLYKEYG IFVEFYKNIY AQYHKSYPEI FNSSIETKPK GLTYADLIRV FNRALTLEVY NFNKFSLRKI ENMLNQKGLN TDFSIDQAIE QENFELLISL LNYDNTKIQF YTVNKAIEKG NLDILKAVLE HSTTNIIPYN IERVIEKGNL DILKAVLEHS TIKIESNNVD TAIEKGNLDL LKAVLEHNTT KIESYNIDRA IVKGNLDIFK AVLEHSTTKI ESYNFDKIIE KGNLDIFKAA LEHSTIKIES YNFDKIIEKG NLDIVKAVLA HSTTKIESYN INKAIEKSNL DILKAVLEHS TAKIESYNIE RAIEKGNLDI LKAVLEHSTA KMYSYNFDTV IKTGNLEMLD MVLQHLDIAV DSYNIYVAQR TNNLEVIELI KKYHNHTINN IMPEVYSSIE EDVTILGAEI VQ
NT Sequence
atgcagaaaa tattacttca aggttatgat ataaatatga ataatacaaa tcctgatatt aaggaaatga gaaccttaaa tgacttacct tcggctaaag cttgtgaata ttttatatat gcacatggtt cagagttttt ttatcaaaat tctcatttag gtttattagg aagaattcag ttatatttta tgcctgatcc ttcttacttg tctgtaagta tattatctga tgaaggtgta acttctatgt tggatattac actgctaaat aaaatacaac gtaatacccc tgtaaatcaa tgtaatattg ttcatatatt ttcttgtcat tcaggtgcag cgcaacatca tttaaatttt atagaaggga atattgtttt atgcacttat aataaagcaa gtgatagtaa tgttatacaa ttagctaaga gtaattatga gactagaaca aaaaatcact cttctctgat agaatatata catgataatt ttcatttatt agtcgcctca gattttagca taagttataa attagataat aaaatttata atttttcttt cagtgctgat aaaattaaaa acatgaaaag catagaagag ttaccttctt ttttatataa agaatatgga atttttgtag agttttataa aaatatatat gcacaatatc ataaatcata tccagaaata tttaacagca gtatagaaac aaagccgaaa gggttaacat acgctgattt aataagagta tttaatagag ctttaacttt agaagtatat aattttaata aattttctct aagaaagata gagaatatgt taaatcagaa aggattaaat actgatttta gtatagatca agcgatagag caagaaaatt ttgaattatt aatttcatta cttaattatg ataatactaa aatacagttc tatactgtta ataaggctat agagaaaggc aatttagata ttctaaaagc tgtacttgaa catagcacta caaatataat accttataat attgagaggg ttatcgagaa aggcaattta gatattctaa aagctgtact tgaacatagc actataaaga tagaatctaa taatgttgat actgctatag agaaaggcaa tttagatctt ttaaaagctg tacttgaaca taatactaca aagatagaat cttataatat tgatagggct atagtgaaag gtaatttaga tatttttaaa gctgtacttg aacatagtac tacaaagata gaatcttata attttgataa gattattgag aaaggtaatt tagatatttt taaagctgcg cttgaacata gtactataaa gatagaatct tataattttg ataagattat tgagaaaggc aatttagata ttgtaaaagc tgtacttgcg catagcacta caaagatcga atcttacaat attaataagg ctatagagaa aagcaattta gatattttaa aagctgtact tgaacatagt actgcaaaga tagaatctta taatattgag cgggctatag agaaaggtaa tttagatatt ttaaaagctg tacttgaaca tagtactgca aagatgtact cttataattt tgatacagtt ataaaaactg gtaatctaga aatgctagat atggtacttc aacatttgga tatagcggta gatagctata atatttatgt agcacaacgt actaataatt tggaagttat tgaattaata aaaaaatatc ataatcatac tataaataat attatgcctg aagtctattc ttctatagaa gaagatgtta caatattggg agctgaaata gtgcagtaa
Details for RityA.18707.a.B11.GE38035
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/5/2014
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MQKILLQGYD INMNNTNPDI KEMRTLNDLP SAKACEYFIY AHGSEFFYQN SHLGLLGRIQ LYFMPDPSYL SVSILSDEGV TSMLDITLLN KIQRNTPVNQ CNIVHIFSCH SGAAQHHLNF IEGNIVLCTY NKASDSNVIQ LAKSNYETRT KNHSSLIEYI HDNFHLLVAS DFSISYKLDN KIYNFSFSAD KIKNMKSIEE LPSFLYKEYG IFVEFYKNIY AQYHKSYPEI FNSSIETKPK GLTYADLIRV FNRALTLEVY NFNKFSLRKX ENMLNQKGLN TDFSIDQAIE QENFELLISL LNYDNTKIQF YTVNKAIEKG NLDILKAVLE HSTTNIIPYN IERVIEKGNL DILKAVLEHS TIKIXXNNVD TAIEKGNLDL LKAVLEHNTT KIESYNIDRA IVKGNLDIFK AVLEHSTTKI ESYNFDKIIE KGNLDIFKAA LEHSTIKIES YNFDKIIEKG NLDIVKAVLA HSTTKIESYN INKAIEKSNL DILKAVLEHS TAKIESYNIE RAIEKGNLDI LKAVLEHSTA KMYSYNFDTV IKTGNLEMLD MVLQHLDIAV DSYNIYVAQR TNNLEVIELI KKYHNHTINN IMPEVYSSIE EDVTILGAEI VQ
Validated NT Sequence
atcttactca cttactgcac tatttcagct cccaatattg taacatcttc ttctatagaa gaatagactt caggcataat attatttata gtatgattat gatatttttt tattaattca ataacttcca aattattagt acgttgtgct acataaatat tatagctatc taccgctata tccaaatgtt gaagtaccat atctagcatt tctagattac cagtttttat aactgtatca aaattataag agtacatctt tgcagtacta tgttcaagta cagcttttaa aatatctaaa ttacctttct ctatagcccg ctcaatatta taagattcta tctttgcagt actatgttca agtacagctt ttaaaatatc taaattgctt ttctctatag ccttattaat attgtaagat tcgatctttg tagtgctatg cgcaagtaca gcttttacaa tatctaaatt gcctttctca ataatcttat caaaattata agattctatc tttatagtac tatgttcaag cgcagcttta aaaatatcta aattaccttt ctcaataatc ttatcaaaat tataagattc tatctttgta gtactatgtt caagtacagc tttaaaaata tctaaattac ctttcactat agccctatca atattataag attctatctt tgtagtatta tgttcaagta cagcttttaa aagatctaaa ttgcctttct ctatagcagt atcaacatta ttagnntcta tctttatagt gctatgttca agtacagctt ttagaatatc taaattgcct ttctcgataa ccctctcaat attataaggt attatatttg tagtgctatg ttcaagtaca gcttttagaa tatctaaatt gcctttctct atagccttat taacagtata gaactgtatt ttagtattat cataattaag taatgaaatt aataattcaa aattttcttg ctctatcgct tgatctatac taaaatcagt atttaatcct ttctgattta acatattctc tntctttctt agagaaaatt tattaaaatt atatacttct aaagttaaag ctctattaaa tactcttatt aaatcagcgt atgttaaccc tttcggcttt gtttctatac tgctgttaaa tatttctgga tatgatttat gatattgtgc atatatattt ttataaaact ctacaaaaat tccatattct ttatataaaa angaaggtaa ctcttctatg cttttcatgt ttttaatttt atcagcactg aaagaaaaat tataaatttt attatctaat ttataactta tgctaaaatc tgaggcgact aataaatgaa aattatcatg tatatattct atcagagaag agtgattttt tgttctagtc tcataattac tcttagctaa ttgtataaca ttactatcac ttgctttatt ataagtgcat aaaacaatat tcccttctat aaaatttaaa tgatgttgcg ctgcacctga atgacaagaa aatatatgaa caatattaca ttgatttaca ggggtattac gttgtatttt atttagcagt gtaatatcca acatagaagt tacaccttca tcagataata tacttacaga caagtaagaa ggatcaggca taaaatataa ctgaattctt cctaataaac ctaaatgaga attttgataa aaaaactctg aaccatgtgc atatataaaa tattcacaag ctttagccga aggtaagtca tttaaggttc tcatttcctt aatatcagga tttgtattat tcatatttat atcataacct tgaagtaata ttttctgcat atggtggtgg tggtggtgag cca
Expected Protein Sequence
MAHHHHHHMQ KILLQGYDIN MNNTNPDIKE MRTLNDLPSA KACEYFIYAH GSEFFYQNSH LGLLGRIQLY FMPDPSYLSV SILSDEGVTS MLDITLLNKI QRNTPVNQCN IVHIFSCHSG AAQHHLNFIE GNIVLCTYNK ASDSNVIQLA KSNYETRTKN HSSLIEYIHD NFHLLVASDF SISYKLDNKI YNFSFSADKI KNMKSIEELP SFLYKEYGIF VEFYKNIYAQ YHKSYPEIFN SSIETKPKGL TYADLIRVFN RALTLEVYNF NKFSLRKIEN MLNQKGLNTD FSIDQAIEQE NFELLISLLN YDNTKIQFYT VNKAIEKGNL DILKAVLEHS TTNIIPYNIE RVIEKGNLDI LKAVLEHSTI KIESNNVDTA IEKGNLDLLK AVLEHNTTKI ESYNIDRAIV KGNLDIFKAV LEHSTTKIES YNFDKIIEKG NLDIFKAALE HSTIKIESYN FDKIIEKGNL DIVKAVLAHS TTKIESYNIN KAIEKSNLDI LKAVLEHSTA KIESYNIERA IEKGNLDILK AVLEHSTAKM YSYNFDTVIK TGNLEMLDMV LQHLDIAVDS YNIYVAQRTN NLEVIELIKK YHNHTINNIM PEVYSSIEED VTILGAEIVQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga aaatattact tcaaggttat gatataaata tgaataatac aaatcctgat attaaggaaa tgagaacctt aaatgactta ccttcggcta aagcttgtga atattttata tatgcacatg gttcagagtt tttttatcaa aattctcatt taggtttatt aggaagaatt cagttatatt ttatgcctga tccttcttac ttgtctgtaa gtatattatc tgatgaaggt gtaacttcta tgttggatat tacactgcta aataaaatac aacgtaatac ccctgtaaat caatgtaata ttgttcatat attttcttgt cattcaggtg cagcgcaaca tcatttaaat tttatagaag ggaatattgt tttatgcact tataataaag caagtgatag taatgttata caattagcta agagtaatta tgagactaga acaaaaaatc actcttctct gatagaatat atacatgata attttcattt attagtcgcc tcagatttta gcataagtta taaattagat aataaaattt ataatttttc tttcagtgct gataaaatta aaaacatgaa aagcatagaa gagttacctt cttttttata taaagaatat ggaatttttg tagagtttta taaaaatata tatgcacaat atcataaatc atatccagaa atatttaaca gcagtataga aacaaagccg aaagggttaa catacgctga tttaataaga gtatttaata gagctttaac tttagaagta tataatttta ataaattttc tctaagaaag atagagaata tgttaaatca gaaaggatta aatactgatt ttagtataga tcaagcgata gagcaagaaa attttgaatt attaatttca ttacttaatt atgataatac taaaatacag ttctatactg ttaataaggc tatagagaaa ggcaatttag atattctaaa agctgtactt gaacatagca ctacaaatat aataccttat aatattgaga gggttatcga gaaaggcaat ttagatattc taaaagctgt acttgaacat agcactataa agatagaatc taataatgtt gatactgcta tagagaaagg caatttagat cttttaaaag ctgtacttga acataatact acaaagatag aatcttataa tattgatagg gctatagtga aaggtaattt agatattttt aaagctgtac ttgaacatag tactacaaag atagaatctt ataattttga taagattatt gagaaaggta atttagatat ttttaaagct gcgcttgaac atagtactat aaagatagaa tcttataatt ttgataagat tattgagaaa ggcaatttag atattgtaaa agctgtactt gcgcatagca ctacaaagat cgaatcttac aatattaata aggctataga gaaaagcaat ttagatattt taaaagctgt acttgaacat agtactgcaa agatagaatc ttataatatt gagcgggcta tagagaaagg taatttagat attttaaaag ctgtacttga acatagtact gcaaagatgt actcttataa ttttgataca gttataaaaa ctggtaatct agaaatgcta gatatggtac ttcaacattt ggatatagcg gtagatagct ataatattta tgtagcacaa cgtactaata atttggaagt tattgaatta ataaaaaaat atcataatca tactataaat aatattatgc ctgaagtcta ttcttctata gaagaagatg ttacaatatt gggagctgaa atagtgcagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat