RityA.18412.a

RvhB4-II; VirB4 protein

CENTER ID: RityA.18412.a
ORGANISM: Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE: Murine typhus (endemic typhus)
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
RityA.18412.a.B21.GE41219 hydrolase domain 392 810
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|257363.4.peg.791
RefSeq: WP_011191202.1
UniProt: Q68VW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFSDLRKFDK DIYNYNAPNF IPIACHYNEN TLLTKDGKLL QIVKIHGINS EKISDNIQNL REMVRLAIKK HITDYDFAFW LHTIREKQNL DDTTPYKKLL PANIHTLWQR KNHWNDKFVN TLYISIIHDS AKIQIKNFSS LINSLSYKLI TNFENTYLDS AFKKLENITN NILDDLNEFG AEKLGIIFEN DNVFSNTLFL YNRIANLNDN DCLVPIIDLS NALGRSIYTI SGDKIVVTDH AKNNKFVSLL SIKEYQETPS NTLDKFLQLP IELIITEIFY FVSKKQIISK LHGQDHILKI TNDSILLNHK GINKFDEANV DFQFCNQQIS IAVIEEDENK LAKAVAKAST ELFKLGIIHV KEDINIEQIF WSQLPANFAF IRRISPLSVE HIASFTALHN TTLGNQYNPW GRAITLLRTE KGTPYFMNFH DKTNKGNTCI FGTEKTGKTV LLNFLISEST KYEPTIIYIS NNNDSKIFIE AIEGKWLEPD KQIINPFLVD DTEQSQAFIL EFLKLISGHY ISPLSEIEIS FLEKLKNKIL SIEKEKRIFS NILKLANFKE EGGIQIFDKL KVFTEGQLYY GLFDGPSLNI KEGEVIGFNL YTLSDEPFSK QFYPMERKFL EQFNHNLKKH QSISAAVIYA LSYNLSILGT KPKIFAADNF DQLYRPESYY DNINLIYNNL SENNGIFVSN FNFIYLKSYP KYTIKPWLDL INTKIILPSD VKIEDLDKIL GLSELEIRKL SQLILSARMF LINKDNESIA SELSIAALIG IVRILSSRQE EMDIYKKILQ EHNGPPDNWI NYLYNALNIY
NT Sequence
gtgtttagtg atttacggaa atttgacaaa gatatatata attataatgc gcctaatttt atacctatag catgtcatta taatgaaaat actttactta ccaaagacgg taaattacta caaattgtaa aaatacacgg tattaactca gagaaaataa gcgacaatat acaaaattta cgtgaaatgg ttagacttgc tataaaaaag catataaccg attatgattt tgctttttgg ctacacacaa tacgagaaaa acaaaactta gacgatacca cgccttataa aaaattatta ccagccaata tacatacact atggcaaaga aaaaaccatt ggaatgataa attcgttaat actttatata tatctataat acatgattct gctaaaattc agatcaaaaa ttttagttct ttaataaatt cattatctta taaattaatt actaattttg aaaatacata cttagattca gcttttaaaa aactagaaaa tatcaccaat aatattttgg acgatttaaa tgaatttggg gctgaaaaac ttgggataat atttgaaaat gataatgttt tttctaatac tttatttcta tataatcgca tagctaatct taacgataat gattgtttgg tgcctataat agatttatcc aatgcattag gtagaagcat ttatacaatt agcggcgata aaatagtagt tacagatcat gcaaaaaata acaaatttgt ttctttatta tctatcaaag aatatcaaga aactccttct aatacactgg ataaattttt gcaattaccg attgaattaa taataactga aatattttac tttgttagca aaaaacaaat aatatccaaa ttgcatggtc aagatcatat tcttaaaatt actaacgact caattttact taatcacaaa ggaattaata aatttgatga agctaacgta gattttcaat tttgtaacca gcaaatttcg attgcagtta tagaagaaga tgaaaataaa ctagccaaag ctgtagcaaa agcatcaaca gaactcttta agcttggtat tattcatgta aaagaagata ttaatatcga gcaaatattt tggtcacaat tacctgctaa ctttgctttt atacgcagaa tatcaccact atctgttgaa catattgcgt cttttactgc tctacataat actacactcg gtaatcaata taatccctgg ggcagggcta taactttact gcgaactgaa aaaggtacac catatttcat gaattttcat gataaaacaa ataaaggtaa tacttgcatc tttggtacag aaaaaactgg caaaaccgta ttattgaact ttttaatatc agaatcaacc aaatatgaac caacaataat ttatatttct aataataatg attctaaaat ttttattgag gcaatagaag gcaaatggtt agaaccggac aaacaaatta tcaatccatt tttagtagat gatacagaac aatcacaagc tttcatttta gaatttttaa aattaatcag tggtcattat atttcaccac ttagtgaaat cgagatatct tttctagaaa aattgaaaaa taaaatttta tcaattgaaa aagaaaaacg tattttttct aacattttaa aattagcaaa ttttaaagag gaaggaggaa tacaaatatt tgataaactt aaagttttta ctgaaggaca attatattac ggattatttg atgggccatc tcttaacatt aaagaaggcg aagtgatagg gtttaactta tatacacttt ctgatgagcc gttttctaaa caattctatc caatggagag aaaattttta gaacaattta atcataattt aaaaaaacac caaagtatta gtgcagcagt aatttatgct ttgagttata atttaagcat acttggcaca aaacctaaaa tatttgctgc tgataatttt gatcaacttt acagacctga atcttattat gataatataa atttaatcta taataattta tcagaaaata atggtatttt tgtcagtaat ttcaatttta tttatcttaa atcatatcca aaatatacta taaaaccttg gcttgattta attaatacta aaattatact gccctctgac gttaaaatag aagatttaga taaaatttta ggtttaagtg aattggaaat acgaaaatta tcacagctaa tactttctgc aagaatgttc ttaattaata aagataatga atcaattgct tctgaattaa gtattgcagc tttaataggt attgtacgta ttttatcaag cagacaagag gaaatggata tttataaaaa aatacttcaa gaacataacg gtcctccaga taattggatt aattatctat ataatgcgtt aaatatatac taa
Details for RityA.18412.a.B21.GE41219
HARVESTED ON: 3/1/2017
SEQUENCED ON: 3/2/2017
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHIA SFTALHNTTL GNQYNPWGRA ITLLRTEKGT PYFMNFHDKT NKGNTCIFGT EKTGKTVLLN FLISESTKYE PTIIYISNNN DSKIFIEAIE GKWLEPDKQI INPFLVDDTE QSQAFILEFL KLISGHYISP LSEIEISFLE KLKNKILSIE KEKRIFSNIL KLANFKEEGG IQIFDKLKVF TEGQLYYGLF DGPSLNIKEG EVIGFNLYTL SDEPFSKQFY PMERKFLEQF NHNLKKHQSI SAAVIYALSY NLSILGTKPK IFAADNFDQL YRPESYYDNI NLIYNNLSEN NGIFVSNFNF IYLKSYPKYT IKPWLDLINT KIILPSDVKI EDLDKILGLS ELEIRKLSQL ILSARMFLIN KDNESIASEL SIAALIGIVR ILSSRQEEMD IYKKILQEHN GPPDNWINYL YNALNIY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catattgcgt catttaccgc cctgcacaat accacgctgg gcaaccagta taatccgtgg ggtcgtgcca ttacgctgct gcgcaccgaa aaaggcacgc cgtacttcat gaacttccat gataaaacca acaaaggtaa tacgtgcatt tttggcaccg aaaaaacggg taaaaccgtt ctgctgaatt tcctgatcag cgaatctacc aaatatgaac cgacgattat ctacatctcc aacaacaacg attcaaaaat cttcatcgaa gcgatcgaag gcaaatggct ggaaccggac aaacagatta tcaacccgtt cctggtcgat gacaccgaac agtcccaagc ctttattctg gaattcctga aactgatctc aggtcactat attagtccgc tgtccgaaat tgaaatcagc tttctggaaa aactgaaaaa caaaatcctg tctatcgaaa aagaaaaacg tatctttagt aacattctga aactggccaa tttcaaagaa gaaggcggta tccagatctt cgataaactg aaagtcttca ccgaaggtca actgtattac ggcctgtttg acggtccgag cctgaacatc aaagaaggcg aagtgattgg tttcaatctg tataccctgt cggatgaacc gtttagcaaa cagttctacc cgatggaacg taaatttctg gaacagttca accataatct gaaaaaacac caaagtatct ccgcggccgt gatttatgca ctgtcctaca acctgtcaat cctgggcacc aaaccgaaaa tttttgcagc tgataatttc gaccagctgt atcgcccgga atcttactac gataacatca acctgatcta caacaacctg tcggaaaata acggtatctt tgtgagcaac ttcaacttca tctacctgaa aagttacccg aaatacacca tcaaaccgtg gctggatctg attaacacga aaattatcct gccgtcggac gttaaaatcg aagatctgga caaaattctg ggcctgagcg aactggaaat ccgtaaactg tctcagctga ttctgagtgc gcgcatgttc ctgatcaaca aagataacga atcaatcgcc tcggaactga gcattgcggc cctgattggt atcgttcgta ttctgagttc ccgccaggaa gaaatggata tctataagaa aattctgcaa gaacacaatg gcccgccgga taactggatt aactacctgt ataacgcact gaacatctac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat