SSGCID: Target Page

RityA.18408.a

RvhB4-I; VirB4 protein

CENTER ID:	RityA.18408.a
ORGANISM:	Rickettsia typhi str. Wilmington
ASSOCIATED DISEASE:	Murine typhus (endemic typhus)
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB/C

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
RityA.18408.a.B1.GE40975	full length		1	805
RityA.18408.a.B21.GE41218	hydrolase domain		408	805

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|257363.4.peg.31
RefSeq:	WP_011190508.1
UniProt:	Q68XX1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLFRTRTAK ELRSKQERPT SHFIPYRCHW DSNTILTKDN SLLQVIKING FSFETADDED LDIKKNIRNA LLKNMASGNI VMYFHTIRRR KAVIFDDTEF TYDPTVKVPN DFITYLGAEW RKKHAGAKSF FNELYISILY KPDTGGAAIV EYFLKKLRQK SNKTAWENDM KEMKENLQEM STRVINTFRS YGAKLLGVRQ TQSGIYCEIL EFLSSLINCG DSPGPIALPR GTIDEYLPTH RLFFDSRTIE VRSPFGKKYA GIISILEYGP NTSAGIFDGF LQMPFEFVMT QSFVFANRTV AIGKMKLQQN RMIQSGDKAT SQIVEINTAL DMATSGDIGF GDHHLSLLCS AKNIKALEDI LSMAAVELSN SGIQPVREKV NMEPSYWGQL PGNMDYIVRK STINTLNMAS FASQHNYPLG KIRDNHWGEY VTVLDTTSGT PFYFNFHVRD VGHTLIIGPT GAGKTVLMNF LCAEAQKFKP RMFFFDKDRG AEIFIRALNG VYTVIDPGLK CNFNPLHLED TSENRTFILE WLRVLVTSNG ESLTAQDNKI LSQAVSGNFR LEKKDRRLSN VIAFLGIDTP NSLASRIAMW VGKGSHAKIF DNEIDDIDLQ RARVFGFDMT ELLKDPVSLA PVLLYIFHRI NISLDGQKTM IVLDEAWALI DNPVFAPKIK DWLKVLRKLN TFVIFATQSV EDAAKSSISD TLIQQTATQI FLPNLKATDI YRSAFMLSQR EYILIKTTDP TTRYFLIKQG IDAVVAKVNL EGMDNIISVL SGRVETVILL DQIRDKYGND PDKWLPIFYE AVKTL

NT Sequence

atgaagttat ttagaactag aacagctaaa gaattaaggt ctaaacaaga aagaccaact tcgcatttta tcccttatag gtgtcattgg gatagtaata ctattttaac aaaagataat tctttattac aagttattaa aatcaatgga ttttcttttg aaactgccga tgatgaagat ttagacatta aaaagaatat cagaaatgcc ttgcttaaaa atatggcttc tggaaacatt gttatgtatt tccatactat tagaagacgt aaagcagtaa tatttgacga tacagaattt acttatgatc ctaccgtaaa agtacctaat gattttatta cttatttagg tgcagagtgg cgtaagaaac atgcaggtgc taaatcattt tttaatgaat tatatattag tattttatat aagcctgata ctggcggtgc tgctatagtt gagtattttt taaagaagct tagacagaaa tctaataaaa ctgcttggga aaatgacatg aaagagatga aagaaaatct tcaagaaatg tcaacgagag tgattaatac attcagaagt tatggagcaa aattacttgg agtccgtcaa acacagtcag gtatctattg tgaaatttta gaatttcttt catctttaat taattgcggt gattcaccag gacctatagc attaccgcgt ggtactattg atgaatattt accaacacat agattatttt ttgattctcg tactattgaa gtcagaagtc cttttggcaa gaaatatgct ggcatcatca gtatacttga atacggtcct aatacttctg cgggaatttt tgatggattt ctacaaatgc cttttgaatt tgttatgact caaagctttg tatttgctaa tagaactgta gcaattggta aaatgaaatt acagcaaaat agaatgatac aatccggtga taaggcgact tcacaaattg ttgaaatcaa tacagcactt gatatggcta ctagcggtga tattggtttt ggagatcatc atttatcact tttatgttcc gcaaagaata ttaaagcttt agaagatata ttatcaatgg cagcagttga gctttctaat tcaggaattc agccggtgag agaaaaagtg aacatggaac ctagctattg gggtcaatta cctggaaata tggattatat agtgcgtaaa tcgactataa atactcttaa tatggctagc tttgcttcgc aacataatta tccactcggt aaaattagag ataaccattg gggagaatat gtcacagtgc ttgatacaac ttcaggtaca ccgttttatt ttaattttca tgttagagat gtgggacata ctctaattat aggtccaacg ggtgccggta aaacagtgct tatgaacttc ttatgtgctg aagcacaaaa attcaaacct cgtatgttct tttttgataa agatcgcgga gcagaaatat ttatacgtgc tttaaatgga gtttatacag taattgatcc tggtttaaaa tgcaacttta atcctttgca tcttgaagat actagtgaga atagaacgtt tattttagaa tggcttcgcg tacttgtaac ttctaacggt gaaagtttaa ctgcacaaga taataaaatc ttatcacaag ctgtaagtgg caattttaga ttagaaaaaa aagacagaag gcttagtaac gttattgcat tccttggtat tgatacacca aatagtttag caagtaggat tgcaatgtgg gttggtaaag gttcacacgc taagatattt gataatgaaa tagatgatat cgatttacaa agagcaaggg tatttggttt tgatatgacg gaattactta aagatcctgt aagtcttgca ccggtattgt tatatatttt tcatcgtatt aatatatctt tagatggaca aaaaacgatg atagtacttg atgaagcatg ggctttaatt gacaatccag tatttgcgcc taagatcaaa gattggttaa aagtgttgag aaaattaaat acttttgtta tatttgctac gcagagtgta gaagatgctg caaaaagtag tattagtgat acgttgattc aacaaacagc cacacagatt tttttaccta atttaaaagc tacggatatt tatcgtagtg catttatgtt aagtcagcgc gaatatattt taattaaaac taccgatcct actacacgtt attttttaat aaaacaagga atagatgccg tagttgcgaa agttaactta gaaggtatgg ataatataat tagtgtttta tctggcagag ttgaaactgt aatattgctt gatcaaatta gagataaata cggcaatgat ccggataagt ggttacctat attttatgaa gcagttaaaa cattatag

Details for RityA.18408.a.B1.GE40975

HARVESTED ON:	12/20/2016
SEQUENCED ON:	12/27/2016
EXPECTED MW:	92kDa
OBSERVED MW:	92kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK LFRTRTAKEL RSKQERPTSH FIPYRCHWDS NTILTKDNSL LQVIKINGFS FETADDEDLD IKKNIRNALL KNMASGNIVM YFHTIRRRKA VIFDDTEFTY DPTVKVPNDF ITYLGAEWRK KHAGAKSFFN ELYISILYKP DTGGAAIVEY FLKKLRQKSN KTAWENDMKE MKENLQEMST RVINTFRSYG AKLLGVRQTQ SGIYCEILEF LSSLINCGDS PGPIALPRGT IDEYLPTHRL FFDSRTIEVR SPFGKKYAGI ISILEYGPNT SAGIFDGFLQ MPFEFVMTQS FVFANRTVAI GKMKLQQNRM IQSGDKATSQ IVEINTALDM ATSGDIGFGD HHLSLLCSAK NIKALEDILS MAAVELSNSG IQPVREKVNM EPSYWGQLPG NMDYIVRKST INTLNMASFA SQHNYPLGKI RDNHWGEYVT VLDTTSGTPF YFNFHVRDVG HTLIIGPTGA GKTVLMNFLC AEAQKFKPRM FFFDKDRGAE IFIRALNGVY TVIDPGLKCN FNPLHLEDTS ENRTFILEWL RVLVTSNGES LTAQDNKILS QAVSGNFRLE KKDRRLSNVI AFLGIDTPNS LASRIAMWVG KGSHAKIFDN EIDDIDLQRA RVFGFDMTEL LKDPVSLAPV LLYIFHRINI SLDGQKTMIV LDEAWALIDN PVFAPKIKDW LKVLRKLNTF VIFATQSVED AAKSSISDTL IQQTATQIFL PNLKATDIYR SAFMLSQREY ILIKTTDPTT RYFLIKQGID AVVAKVNLEG MDNIISVLSG RVETVILLDQ IRDKYGNDPD KWLPIFYEAV KTL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaagt tatttagaac tagaacagct aaagaattaa ggtctaaaca agaaagacca acttcgcatt ttatccctta taggtgtcat tgggatagta atactatttt aacaaaagat aattctttat tacaagttat taaaatcaat ggattttctt ttgaaactgc cgatgatgaa gatttagaca ttaaaaagaa tatcagaaat gccttgctta aaaatatggc ttctggaaac attgttatgt atttccatac tattagaaga cgtaaagcag taatatttga cgatacagaa tttacttatg atcctaccgt aaaagtacct aatgatttta ttacttattt aggtgcagag tggcgtaaga aacatgcagg tgctaaatca ttttttaatg aattatatat tagtatttta tataagcctg atactggcgg tgctgctata gttgagtatt ttttaaagaa gcttagacag aaatctaata aaactgcttg ggaaaatgac atgaaagaga tgaaagaaaa tcttcaagaa atgtcaacga gagtgattaa tacattcaga agttatggag caaaattact tggagtccgt caaacacagt caggtatcta ttgtgaaatt ttagaatttc tttcatcttt aattaattgc ggtgattcac caggacctat agcattaccg cgtggtacta ttgatgaata tttaccaaca catagattat tttttgattc tcgtactatt gaagtcagaa gtccttttgg caagaaatat gctggcatca tcagtatact tgaatacggt cctaatactt ctgcgggaat ttttgatgga tttctacaaa tgccttttga atttgttatg actcaaagct ttgtatttgc taatagaact gtagcaattg gtaaaatgaa attacagcaa aatagaatga tacaatccgg tgataaggcg acttcacaaa ttgttgaaat caatacagca cttgatatgg ctactagcgg tgatattggt tttggagatc atcatttatc acttttatgt tccgcaaaga atattaaagc tttagaagat atattatcaa tggcagcagt tgagctttct aattcaggaa ttcagccggt gagagaaaaa gtgaacatgg aacctagcta ttggggtcaa ttacctggaa atatggatta tatagtgcgt aaatcgacta taaatactct taatatggct agctttgctt cgcaacataa ttatccactc ggtaaaatta gagataacca ttggggagaa tatgtcacag tgcttgatac aacttcaggt acaccgtttt attttaattt tcatgttaga gatgtgggac atactctaat tataggtcca acgggtgccg gtaaaacagt gcttatgaac ttcttatgtg ctgaagcaca aaaattcaaa cctcgtatgt tcttttttga taaagatcgc ggagcagaaa tatttatacg tgctttaaat ggagtttata cagtaattga tcctggttta aaatgcaact ttaatccttt gcatcttgaa gatactagtg agaatagaac gtttatttta gaatggcttc gcgtacttgt aacttctaac ggtgaaagtt taactgcaca agataataaa atcttatcac aagctgtaag tggcaatttt agattagaaa aaaaagacag aaggcttagt aacgttattg cattccttgg tattgataca ccaaatagtt tagcaagtag gattgcaatg tgggttggta aaggttcaca cgctaagata tttgataatg aaatagatga tatcgattta caaagagcaa gggtatttgg ttttgatatg acggaattac ttaaagatcc tgtaagtctt gcaccggtat tgttatatat ttttcatcgt attaatatat ctttagatgg acaaaaaacg atgatagtac ttgatgaagc atgggcttta attgacaatc cagtatttgc gcctaagatc aaagattggt taaaagtgtt gagaaaatta aatacttttg ttatatttgc tacgcagagt gtagaagatg ctgcaaaaag tagtattagt gatacgttga ttcaacaaac agccacacag atttttttac ctaatttaaa agctacggat atttatcgta gtgcatttat gttaagtcag cgcgaatata ttttaattaa aactaccgat cctactacac gttatttttt aataaaacaa ggaatagatg ccgtagttgc gaaagttaac ttagaaggta tggataatat aattagtgtt ttatctggca gagttgaaac tgtaatattg cttgatcaaa ttagagataa atacggcaat gatccggata agtggttacc tatattttat gaagcagtta aaacattatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for RityA.18408.a.B21.GE41218

HARVESTED ON:	3/1/2017
SEQUENCED ON:	3/2/2017
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMA SFASQHNYPL GKIRDNHWGE YVTVLDTTSG TPFYFNFHVR DVGHTLIIGP TGAGKTVLMN FLCAEAQKFK PRMFFFDKDR GAEIFIRALN GVYTVIDPGL KCNFNPLHLE DTSENRTFIL EWLRVLVTSN GESLTAQDNK ILSQAVSGNF RLEKKDRRLS NVIAFLGIDT PNSLASRIAM WVGKGSHAKI FDNEIDDIDL QRARVFGFDM TELLKDPVSL APVLLYIFHR INISLDGQKT MIVLDEAWAL IDNPVFAPKI KDWLKVLRKL NTFVIFATQS VEDAAKSSIS DTLIQQTATQ IFLPNLKATD IYRSAFMLSQ REYILIKTTD PTTRYFLIKQ GIDAVVAKVN LEGMDNIISV LSGRVETVIL LDQIRDKYGN DPDKWLPIFY EAVKTL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcga gctttgcctc tcagcataac tatccgctgg gcaaaattcg cgataatcac tggggtgaat acgttaccgt cctggacacc acgtctggca cgccgttcta ttttaacttc catgtccgtg atgtgggcca caccctgatt atcggtccga ccggcgcggg taaaacggtg ctgatgaatt ttctgtgcgc ggaagcccag aaattcaaac cgcgcatgtt tttctttgat aaagaccgcg gtgcagaaat ttttatccgt gctctgaacg gcgtttatac cgtcattgat ccgggtctga aatgtaactt taatccgctg cacctggaag acaccagcga aaatcgcacg ttcattctgg aatggctgcg tgtgctggtt accagtaacg gcgaatccct gacggcacag gataataaaa tcctgtcaca agctgtttcg ggtaactttc gcctggaaaa gaaagatcgc cgtctgagta acgtcattgc gttcctgggc atcgacaccc cgaatagtct ggcatcccgt attgctatgt gggttggcaa aggttcccat gcaaaaatct tcgataacga aatcgatgac atcgacctgc agcgcgctcg tgtgtttggc ttcgatatga ccgaactgct gaaagacccg gtttcactgg ccccggtcct gctgtacatt tttcaccgca ttaatatctc gctggatggt cagaaaacca tgatcgtgct ggatgaagca tgggctctga ttgacaaccc ggtcttcgcg ccgaaaatca aagattggct gaaagtgctg cgtaaactga atacctttgt gattttcgcc acgcagagtg ttgaagatgc ggccaaaagt tccatttccg acaccctgat ccagcaaacc gcgacgcaga tttttctgcc gaatctgaaa gcgacggata tctatcgcag cgccttcatg ctgtctcaac gtgaatacat tctgatcaaa accacggatc cgaccacgcg ttattttctg attaaacagg gtatcgatgc agtggttgct aaagttaacc tggaaggcat ggacaatatt atctcagtcc tgtcgggtcg cgtggaaacc gttattctgc tggatcagat ccgtgacaaa tacggtaacg acccggataa atggctgccg atcttctatg aagcggtgaa aaccctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at